Restriction map for pFN21AE4367
 (Linear) (Six-base) MAPSORT of: KIEE4367.seq  Check: 6384  from: 1  to: 1158



KIEE4367.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     989    1158 
   Size:        989     169
 
AfeI AGC'GCT

Cuts at:      0      39    1158 
   Size:         39    1119
 
AflII C'TTAA_G

Cuts at:      0     112    1158 
   Size:        112    1046
 
AflIII A'CryG_T

Cuts at:      0     469     756    1158 
   Size:        469     287     402

  Fragments arranged by size: 

                 469     402     287
 
AhdI GACnn_n'nnGTC

Cuts at:      0      19    1158 
   Size:         19    1139
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     468     500    1158 
   Size:        468      32     658

  Fragments arranged by size: 

                 658     468      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     897     948     967    1141    1158 
   Size:        897      51      19     174      17

  Fragments arranged by size: 

                 897     174      51      19      17
 
ApaLI G'TGCA_C

Cuts at:      0     294     760    1158 
   Size:        294     466     398

  Fragments arranged by size: 

                 466     398     294
 
ApoI r'AATT_y

Cuts at:      0     703    1158 
   Size:        703     455
 
AvaI C'yCGr_G

Cuts at:      0      33      41    1158 
   Size:         33       8    1117

  Fragments arranged by size: 

                1117      33       8
 
BanII G_rGCy'C

Cuts at:      0    1105    1158 
   Size:       1105      53
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     117    1158 
   Size:        117    1041
 
Bme1580I G_kGCm'C

Cuts at:      0     298     764    1158 
   Size:        298     466     394

  Fragments arranged by size: 

                 466     394     298
 
BmgBI CAC'GTC

Cuts at:      0     470    1158 
   Size:        470     688
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     575    1158 
   Size:        575     583
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1155    1158 
   Size:       1155       3
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299    1158 
   Size:        299     859
 
Bpu10I CC'TnA_GC

Cuts at:      0     732    1158 
   Size:        732     426
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217    1158 
   Size:        217     941
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     826     873    1158 
   Size:        826      47     285

  Fragments arranged by size: 

                 826     285      47
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      77     107     229     259    1000    1030    1158 
   Size:         77      30     122      30     741      30     128

  Fragments arranged by size: 

                 741     128     122      77      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     253     256     601     758     949     996     999    1158 
   Size:        253       3     345     157     191      47       3     159

  Fragments arranged by size: 

                 345     253     191     159     157      47       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     194     576    1158 
   Size:        194     382     582

  Fragments arranged by size: 

                 582     382     194
 
BsiEI CG_ry'CG

Cuts at:      0      29    1158 
   Size:         29    1129
 
BsiHKAI G_wGCw'C

Cuts at:      0     298     764    1158 
   Size:        298     466     394

  Fragments arranged by size: 

                 466     394     298
 
Bsp1286I G_dGCh'C

Cuts at:      0     298     764    1105    1158 
   Size:        298     466     341      53

  Fragments arranged by size: 

                 466     341     298      53
 
BsrDI GCAATG_nn'

Cuts at:      0     317    1158 
   Size:        317     841
 
BsrFI r'CCGG_y

Cuts at:      0     147    1158 
   Size:        147    1011
 
BsrGI T'GTAC_A

Cuts at:      0     352    1158 
   Size:        352     806
 
BssSI C'ACGA_G

Cuts at:      0     797    1158 
   Size:        797     361
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1141    1158 
   Size:       1141      17
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     495     606    1158 
   Size:        495     111     552

  Fragments arranged by size: 

                 552     495     111
 
BstYI r'GATC_y

Cuts at:      0     107     256    1158 
   Size:        107     149     902

  Fragments arranged by size: 

                 902     149     107
 
BtgI C'CryG_G

Cuts at:      0      88    1158 
   Size:         88    1070
 
DraIII CAC_nnn'GTG

Cuts at:      0     413    1158 
   Size:        413     745
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     924    1158 
   Size:        924     234
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     826    1158 
   Size:        826     332
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299     989    1158 
   Size:        299     690     169

  Fragments arranged by size: 

                 690     299     169
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     235    1158 
   Size:        235     923
 
EcoO109I rG'GnC_Cy

Cuts at:      0     748    1158 
   Size:        748     410
 
HaeII r_GCGC'y

Cuts at:      0      41      54    1158 
   Size:         41      13    1104

  Fragments arranged by size: 

                1104      41      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       5      37      77     109     433     465     482     514 
   Size:          5      32      40      32     324      32      17      32

Cuts at:    514    1000    1032    1158 
   Size:        486      32     126

  Fragments arranged by size: 

                 486     324     126      40      32      32      32      32
                  32      17       5
 
HincII GTy'rAC

Cuts at:      0     159    1158 
   Size:        159     999
 
HindIII A'AGCT_T

Cuts at:      0     432    1158 
   Size:        432     726
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     676    1158 
   Size:        350     326     482

  Fragments arranged by size: 

                 482     350     326
 
MslI CAynn'nnrTG

Cuts at:      0     646    1158 
   Size:        646     512
 
MspA1I CmG'CkG

Cuts at:      0     553     583    1021    1158 
   Size:        553      30     438     137

  Fragments arranged by size: 

                 553     438     137      30
 
NcoI C'CATG_G

Cuts at:      0      88    1158 
   Size:         88    1070
 
NspI r_CATG'y

Cuts at:      0    1072    1158 
   Size:       1072      86
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      89    1098    1158 
   Size:         89    1009      60

  Fragments arranged by size: 

                1009      89      60
 
PfoI T'CCnGG_A

Cuts at:      0      57      95     437    1158 
   Size:         57      38     342     721

  Fragments arranged by size: 

                 721     342      57      38
 
PpuMI rG'GwC_Cy

Cuts at:      0     748    1158 
   Size:        748     410
 
PspXI vC'TCGA_Gb

Cuts at:      0      33      41    1158 
   Size:         33       8    1117

  Fragments arranged by size: 

                1117      33       8
 
PvuII CAG'CTG

Cuts at:      0     553    1158 
   Size:        553     605
 
RsrII CG'GwC_CG

Cuts at:      0     150    1158 
   Size:        150    1008
 
SfcI C'TryA_G

Cuts at:      0     920    1158 
   Size:        920     238
 
SmlI C'TyrA_G

Cuts at:      0      33      41     112     232    1158 
   Size:         33       8      71     120     926

  Fragments arranged by size: 

                 926     120      71      33       8
 
StyI C'CwwG_G

Cuts at:      0      88     885    1158 
   Size:         88     797     273

  Fragments arranged by size: 

                 797     273      88
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     877    1158 
   Size:        877     281
 
TatI w'GTAC_w

Cuts at:      0     352    1158 
   Size:        352     806
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     229     261    1158 
   Size:        229      32     897

  Fragments arranged by size: 

                 897     229      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      72    1158 
   Size:         72    1086
 
XhoI C'TCGA_G

Cuts at:      0      33      41    1158 
   Size:         33       8    1117

  Fragments arranged by size: 

                1117      33       8
 
XmnI GAAnn'nnTTC

Cuts at:      0     703    1082    1158 
   Size:        703     379      76

  Fragments arranged by size: 

                 703     379      76
 

 Enzymes that do cut:

     AcuI     AfeI    AflII   AflIII     AhdI     AloI    AlwNI    ApaLI
     ApoI     AvaI    BanII     BbsI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstXI
    BstYI     BtgI   DraIII     DrdI     EciI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I   HincII  HindIII     MmeI     MslI   MspA1I     NcoI
     NspI    PflMI     PfoI    PpuMI    PspXI    PvuII    RsrII     SfcI
     SmlI     StyI    TaqII     TatI     TstI  Tth111I     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AgeI     AleI     AlfI
     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI     BmtI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI   BssHII    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
     EaeI     EagI     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI     PsrI     PstI     PvuI     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     XbaI     XcmI     XmaI     ZraI
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