################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmBI BsmI BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MamI MfeI MluI MluNI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccII 45 CGCG CG^CG AccIII 347 TCCGGA T^CCGGA AciI 46 GCGG CCGC(-3/-1) AcsI 454 GAATTT [GA]^AATT[CT] Alw44I 204 GTGCAC G^TGCAC ApaLI 204 GTGCAC G^TGCAC ApoI 454 GAATTT [GA]^AATT[CT] AspHI 204 GTGCAC G[AT]GC[AT]^C AvaII 48 GGTCC G^G[AT]CC BamHI 8 GGATCC G^GATCC BpmI 188 CTGGAG CTGGAG(16/14) BsaWI 347 TCCGGA [AT]^CCGG[AT] BseAI 347 TCCGGA T^CCGGA BseRI 151 GAGGAG GAGGAG(10/8) BsgI 300 CTGCAC GTGCAG(16/14) BsiHKAI 204 GTGCAC G[AT]GC[AT]^C Bsp1407I 262 TGTACA T^GTACA BspEI 347 TCCGGA T^CCGGA BspMI 386 GCAGGT ACCTGC(4/8) BsrGI 262 TGTACA T^GTACA BstF5I 432 GGATG GGATG(2/0) BstUI 45 CGCG CG^CG DraIII 318 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 436 GATATC GAT^ATC FokI 432 GGATG GGATG(9/13) MaeII 424 ACGT A^CGT MaeIII 197 GTAAC ^GT[ACGT]AC MroI 347 TCCGGA T^CCGGA MvnI 45 CGCG CG^CG SspBI 262 TGTACA T^GTACA XcmI 464 CCAATGCCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AfaI 263 GTAC GT^AC 422 GTAC BfaI 64 CTAG C^TAG 395 CTAG BmyI 204 GTGCAC G[AGT]GC[ACT]^C 390 GTGCCC Bsp1286I 204 GTGCAC G[AGT]GC[ACT]^C 390 GTGCCC BstNI 428 CCTGG CC^[AT]GG 559 CCAGG BstYI 8 GGATCC [GA]^GATC[CT] 166 GGATCT EcoRII 428 CCTGG ^CC[AT]GG 559 CCAGG HaeIII 3 GGCC GG^CC 117 GGCC MaeI 64 CTAG C^TAG 395 CTAG MflI 8 GGATCC [GA]^GATC[CT] 166 GGATCT MvaI 428 CCTGG CC^[AT]GG 559 CCAGG RsaI 263 GTAC GT^AC 422 GTAC XhoII 8 GGATCC [GA]^GATC[CT] 166 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AluI AlwI AlwNI AvaI BanI BbvI BcnI BsaJI BsiYI BslI BsmAI BsmFI BsoBI BsrI BssKI Cfr13I DpnII EarI Fnu4HI HapII HinfI HpaII ItaI Ksp632I MboI MboII MnlI MspA1I MspI MwoI NciI NdeII NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI SmaI TseI Tsp509I TspRI XmaCI XmaI