(Circular) (Six-base) MAPSORT of: pFN21AE5337.seq Check: 9500 from: 1 to: 5482 pFN21AE5337 5482 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2382 2382 Size: 5482 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3292 278 Size: 53 83 186 936 1756 2468 Fragments arranged by size: 2468 1756 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2825 1937 Size: 888 4594 Acc65I G'GTAC_C Cuts at: 2803 2803 Size: 5482 AclI AA'CG_TT Cuts at: 1563 3609 3982 1563 Size: 2046 373 3063 Fragments arranged by size: 3063 2046 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2517 2562 2607 2652 2697 3545 4929 5197 Size: 885 45 45 45 45 848 1384 268 Cuts at: 5197 1632 Size: 1917 Fragments arranged by size: 1917 1384 885 848 268 45 45 45 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5285 AflIII A'CryG_T Cuts at: 1203 4381 5358 1203 Size: 3178 977 1327 Fragments arranged by size: 3178 1327 977 AgeI A'CCGG_T Cuts at: 4313 4313 Size: 5482 AhdI GACnn_n'nnGTC Cuts at: 4211 4211 Size: 5482 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5482 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5482 AlwNI CAG_nnn'CTG Cuts at: 2495 2540 2585 2630 2675 3225 4797 2495 Size: 45 45 45 45 550 1572 3180 Fragments arranged by size: 3180 1572 550 45 45 45 45 ApaLI G'TGCA_C Cuts at: 2209 3539 4695 2209 Size: 1330 1156 2996 Fragments arranged by size: 2996 1330 1156 ApoI r'AATT_y Cuts at: 1493 2459 2791 3017 3169 5278 1493 Size: 966 332 226 152 2109 1697 Fragments arranged by size: 2109 1697 966 332 226 152 AseI AT'TA_AT Cuts at: 160 1032 4036 160 Size: 872 3004 1606 Fragments arranged by size: 3004 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 AvaI C'yCGr_G Cuts at: 1870 1943 2518 2608 2653 2698 2807 1870 Size: 73 575 90 45 45 109 4545 Fragments arranged by size: 4545 575 109 90 73 45 45 BamHI G'GATC_C Cuts at: 2013 2812 3179 2013 Size: 799 367 4316 Fragments arranged by size: 4316 799 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2345 2394 2465 2803 4258 Size: 325 217 627 558 49 71 338 1455 Cuts at: 4258 618 Size: 1842 Fragments arranged by size: 1842 1455 627 558 338 325 217 71 49 BanII G_rGCy'C Cuts at: 1391 2801 1391 Size: 1410 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4511 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3673 3707 1903 Size: 34 1736 34 3678 Fragments arranged by size: 3678 1736 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3374 4584 3374 Size: 1210 4272 BclI T'GATC_A Cuts at: 1548 1548 Size: 5482 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4093 136 Size: 107 122 71 631 605 2421 1525 Fragments arranged by size: 2421 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5477 5477 Size: 5482 BlpI GC'TnA_GC Cuts at: 2899 2899 Size: 5482 Bme1580I G_kGCm'C Cuts at: 1790 2213 2399 3543 4699 1790 Size: 423 186 1144 1156 2573 Fragments arranged by size: 2573 1156 1144 423 186 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5482 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3213 4171 442 Size: 416 53 83 399 24 1796 958 1753 Fragments arranged by size: 1796 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5482 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2214 4142 1239 Size: 975 1928 2579 Fragments arranged by size: 2579 1928 975 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3607 4472 4770 3607 Size: 865 298 4319 Fragments arranged by size: 4319 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4145 882 Size: 617 2646 2219 Fragments arranged by size: 2646 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3273 493 Size: 711 2069 2702 Fragments arranged by size: 2702 2069 711 BsaBI GATnn'nnATC Cuts at: 5476 5476 Size: 5482 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3289 3671 4306 275 Size: 53 83 186 936 1756 382 635 1451 Fragments arranged by size: 1756 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2352 3915 4313 4587 4734 5145 2352 Size: 1563 398 274 147 411 2689 Fragments arranged by size: 2689 1563 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5257 Fragments arranged by size: 5257 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2171 1186 Size: 985 4497 BseYI C'CCAG_C Cuts at: 2109 2488 2533 2578 2623 2668 4685 5181 2109 Size: 379 45 45 45 45 2017 496 2410 Fragments arranged by size: 2410 2017 496 379 45 45 45 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2290 1163 Size: 573 554 4355 Fragments arranged by size: 4355 573 554 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2932 3693 4721 1540 Size: 275 186 931 761 1028 2301 Fragments arranged by size: 2301 1028 931 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2213 3543 3628 4699 2213 Size: 1330 85 1071 2996 Fragments arranged by size: 2996 1330 1071 85 BsmI GAATG_Cn' Cuts at: 2993 3086 2993 Size: 93 5389 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2213 2399 2801 3543 3628 4699 1391 Size: 399 423 186 402 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 423 402 399 186 85 BspEI T'CCGG_A Cuts at: 2352 5145 2352 Size: 2793 2689 BspHI T'CATG_A Cuts at: 3371 5101 3371 Size: 1730 3752 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2382 2837 844 Size: 1538 455 3489 Fragments arranged by size: 3489 1538 455 BsrBI CCG'CTC Cuts at: 1394 1823 3369 1394 Size: 429 1546 3507 Fragments arranged by size: 3507 1546 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3977 4151 65 Size: 1183 2729 174 1396 Fragments arranged by size: 2729 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4126 4313 1700 Size: 110 2316 187 2869 Fragments arranged by size: 2869 2316 187 110 BsrGI T'GTAC_A Cuts at: 96 2267 96 Size: 2171 3311 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5482 BssSI C'ACGA_G Cuts at: 3542 4554 3542 Size: 1012 4470 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5482 BstBI TT'CG_AA Cuts at: 4286 4286 Size: 5482 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5482 BstYI r'GATC_y Cuts at: 1972 2013 2171 2812 3179 3564 3581 5033 5119 Size: 41 158 641 367 385 17 1452 86 Cuts at: 5119 5131 5477 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 641 385 367 346 158 86 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3991 Fragments arranged by size: 3991 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4743 Fragments arranged by size: 4743 683 56 BtsI GCAGTG_nn' Cuts at: 2085 2998 3792 3812 2085 Size: 913 794 20 3755 Fragments arranged by size: 3755 913 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5482 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5447 DraI TTT'AAA Cuts at: 2786 2786 Size: 5482 DraIII CAC_nnn'GTG Cuts at: 2328 2328 Size: 5482 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4489 809 Size: 507 3173 1802 Fragments arranged by size: 3173 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2929 3818 5447 Size: 54 1425 50 275 18 1099 889 1629 Cuts at: 5447 8 Size: 43 Fragments arranged by size: 1629 1425 1099 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2929 1537 Size: 275 1117 4090 Fragments arranged by size: 4090 1117 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2493 2538 2583 2628 2673 2719 3412 1353 Size: 1140 45 45 45 45 46 693 3423 Fragments arranged by size: 3423 1140 693 46 45 45 45 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4069 4443 4589 387 Size: 1064 138 2480 374 146 1280 Fragments arranged by size: 2480 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2214 2517 2562 2607 2652 2697 3545 Size: 393 582 303 45 45 45 45 848 Cuts at: 3545 4142 4929 5197 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 848 787 597 582 393 303 268 45 45 45 45 EcoRI G'AATT_C Cuts at: 2791 3169 2791 Size: 378 5104 EcoRV GAT'ATC Cuts at: 2443 2443 Size: 5482 FspI TGC'GCA Cuts at: 3988 3988 Size: 5482 HaeII r_GCGC'y Cuts at: 1678 4629 1678 Size: 2951 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4123 4155 4197 4229 1373 Size: 32 135 32 2551 32 42 32 2626 Fragments arranged by size: 2626 2551 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2826 3072 669 Size: 988 281 888 246 3079 Fragments arranged by size: 3079 988 888 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5482 HpaI GTT'AAC Cuts at: 3072 3072 Size: 5482 KpnI G_GTAC'C Cuts at: 2807 2807 Size: 5482 MfeI C'AATT_G Cuts at: 3081 3081 Size: 5482 MluI A'CGCG_T Cuts at: 5358 5358 Size: 5482 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2265 4596 4780 1305 Size: 960 2331 184 2007 Fragments arranged by size: 2331 2007 960 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3660 Fragments arranged by size: 3660 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3440 3799 3958 518 Size: 648 2274 359 159 2042 Fragments arranged by size: 2274 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2492 2537 2582 2627 2672 2898 3575 4723 Size: 886 45 45 45 45 226 677 1148 Cuts at: 4723 4968 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 886 677 245 226 45 45 45 45 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3991 Fragments arranged by size: 3991 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5482 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5482 NotI GC'GGCC_GC Cuts at: 2929 2929 Size: 5482 NspI r_CATG'y Cuts at: 1132 1229 2840 4385 1132 Size: 97 1611 1545 2229 Fragments arranged by size: 2229 1611 1545 97 PasI CC'CwG_GG Cuts at: 1348 2564 1348 Size: 1216 4266 PciI A'CATG_T Cuts at: 4381 4381 Size: 5482 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5482 PmeI GTTT'AAAC Cuts at: 2786 2786 Size: 5482 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3551 3583 5064 5096 3551 Size: 32 1481 32 3937 Fragments arranged by size: 3937 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5482 PsiI TTA'TAA Cuts at: 3052 3052 Size: 5482 PstI C_TGCA'G Cuts at: 830 2834 830 Size: 2004 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5482 PvuII CAG'CTG Cuts at: 2492 2537 2582 2627 2672 2492 Size: 45 45 45 45 5302 Fragments arranged by size: 5302 45 45 45 45 SalI G'TCGA_C Cuts at: 1936 2824 1936 Size: 888 4594 SbfI CC_TGCA'GG Cuts at: 2834 2834 Size: 5482 ScaI AGT'ACT Cuts at: 3730 5442 3730 Size: 1712 3770 SfcI C'TryA_G Cuts at: 826 1045 2830 3965 4646 4837 826 Size: 219 1785 1135 681 191 1471 Fragments arranged by size: 1785 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5482 SmaI CCC'GGG Cuts at: 1872 2520 2610 2655 2700 2809 1872 Size: 648 90 45 45 109 4545 Fragments arranged by size: 4545 648 109 90 45 45 SmlI C'TyrA_G Cuts at: 820 1017 1943 3586 4487 4749 820 Size: 197 926 1643 901 262 1553 Fragments arranged by size: 1643 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3273 493 Size: 2780 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5482 SphI G_CATG'C Cuts at: 1132 2840 1132 Size: 1708 3774 SspI AAT'ATT Cuts at: 5 52 3406 5 Size: 47 3354 2081 Fragments arranged by size: 3354 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5482 StyI C'CwwG_G Cuts at: 513 1065 2004 2921 513 Size: 552 939 917 3074 Fragments arranged by size: 3074 939 917 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3509 3526 3679 3864 4309 325 Size: 1255 1929 17 153 185 445 1498 Fragments arranged by size: 1929 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2267 3728 5440 Size: 274 80 33 51 1443 290 1461 1712 Cuts at: 5440 96 Size: 138 Fragments arranged by size: 1712 1461 1443 290 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5442 5474 5442 Size: 32 5450 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5482 XbaI T'CTAG_A Cuts at: 2818 2818 Size: 5482 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2476 2476 Size: 5482 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5482 XmaI C'CCGG_G Cuts at: 1870 2518 2608 2653 2698 2807 1870 Size: 648 90 45 45 109 4545 Fragments arranged by size: 4545 648 109 90 45 45 XmnI GAAnn'nnTTC Cuts at: 3611 3611 Size: 5482 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3290 276 Size: 53 83 186 936 1756 2468 Fragments arranged by size: 2468 1756 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI