(Linear) (Six-base) MAPSORT of: KIEE5337.seq Check: 3391 from: 1 to: 777 KIEE5337.orf With 182 enzymes: SgfI * January 29, 2014 11:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 377 777 Size: 377 400 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 512 557 602 647 692 777 Size: 512 45 45 45 45 85 Fragments arranged by size: 512 85 45 45 45 45 AlwNI CAG_nnn'CTG Cuts at: 0 490 535 580 625 670 777 Size: 490 45 45 45 45 107 Fragments arranged by size: 490 107 45 45 45 45 ApaLI G'TGCA_C Cuts at: 0 204 777 Size: 204 573 ApoI r'AATT_y Cuts at: 0 454 777 Size: 454 323 AvaI C'yCGr_G Cuts at: 0 513 603 648 693 777 Size: 513 90 45 45 84 Fragments arranged by size: 513 90 84 45 45 BamHI G'GATC_C Cuts at: 0 8 777 Size: 8 769 BanI G'GyrC_C Cuts at: 0 340 389 460 777 Size: 340 49 71 317 Fragments arranged by size: 340 317 71 49 Bme1580I G_kGCm'C Cuts at: 0 208 394 777 Size: 208 186 383 Fragments arranged by size: 383 208 186 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 777 Size: 209 568 BsaWI w'CCGG_w Cuts at: 0 347 777 Size: 347 430 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 166 777 Size: 166 611 BseYI C'CCAG_C Cuts at: 0 104 483 528 573 618 663 777 Size: 104 379 45 45 45 45 114 Fragments arranged by size: 379 114 104 45 45 45 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 285 777 Size: 285 492 BsiHKAI G_wGCw'C Cuts at: 0 208 777 Size: 208 569 Bsp1286I G_dGCh'C Cuts at: 0 208 394 777 Size: 208 186 383 Fragments arranged by size: 383 208 186 BspEI T'CCGG_A Cuts at: 0 347 777 Size: 347 430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 377 777 Size: 377 400 BsrGI T'GTAC_A Cuts at: 0 262 777 Size: 262 515 BstYI r'GATC_y Cuts at: 0 8 166 777 Size: 8 158 611 Fragments arranged by size: 611 158 8 BtsI GCAGTG_nn' Cuts at: 0 80 777 Size: 80 697 DraIII CAC_nnn'GTG Cuts at: 0 323 777 Size: 323 454 EarI CTCTTCn'nnn_ Cuts at: 0 488 533 578 623 668 714 777 Size: 488 45 45 45 45 46 63 Fragments arranged by size: 488 63 46 45 45 45 45 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 512 557 602 647 692 777 Size: 209 303 45 45 45 45 85 Fragments arranged by size: 303 209 85 45 45 45 45 EcoRV GAT'ATC Cuts at: 0 438 777 Size: 438 339 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 260 777 Size: 260 517 MspA1I CmG'CkG Cuts at: 0 487 532 577 622 667 777 Size: 487 45 45 45 45 110 Fragments arranged by size: 487 110 45 45 45 45 PasI CC'CwG_GG Cuts at: 0 559 777 Size: 559 218 PvuII CAG'CTG Cuts at: 0 487 532 577 622 667 777 Size: 487 45 45 45 45 110 Fragments arranged by size: 487 110 45 45 45 45 SmaI CCC'GGG Cuts at: 0 515 605 650 695 777 Size: 515 90 45 45 82 Fragments arranged by size: 515 90 82 45 45 TatI w'GTAC_w Cuts at: 0 262 777 Size: 262 515 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 471 777 Size: 471 306 XmaI C'CCGG_G Cuts at: 0 513 603 648 693 777 Size: 513 90 45 45 84 Fragments arranged by size: 513 90 84 45 45 Enzymes that do cut: AarI AcuI AlwNI ApaLI ApoI AvaI BamHI BanI Bme1580I BpmI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrGI BstYI BtsI DraIII EarI Eco57MI EcoRV MmeI MspA1I PasI PvuII SmaI TatI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TstI Tth111I XbaI XhoI XmnI ZraI