(Circular) (Six-base) MAPSORT of: pFN21AB6147.seq Check: 8260 from: 1 to: 6493 pFN21AB6147 6493 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3009 3009 Size: 6493 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3495 4303 278 Size: 53 83 186 936 1959 808 2468 Fragments arranged by size: 2468 1959 936 808 186 83 53 AccI GT'mk_AC Cuts at: 1937 3836 1937 Size: 1899 4594 Acc65I G'GTAC_C Cuts at: 3814 3814 Size: 6493 AclI AA'CG_TT Cuts at: 1563 4620 4993 1563 Size: 3057 373 3063 Fragments arranged by size: 3063 3057 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2510 2705 3002 3206 3218 3281 4556 5940 Size: 878 195 297 204 12 63 1275 1384 Cuts at: 5940 6208 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1275 878 297 268 204 195 63 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6296 AflIII A'CryG_T Cuts at: 1203 5392 6369 1203 Size: 4189 977 1327 Fragments arranged by size: 4189 1327 977 AgeI A'CCGG_T Cuts at: 2109 5324 2109 Size: 3215 3278 AhdI GACnn_n'nnGTC Cuts at: 3565 5222 3565 Size: 1657 4836 AleI CACnn'nnGTG Cuts at: 1166 2600 1166 Size: 1434 5059 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6493 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2525 2557 2525 Size: 32 6461 AlwNI CAG_nnn'CTG Cuts at: 2488 2800 2812 2842 2995 3010 3076 3196 3310 Size: 312 12 30 153 15 66 120 114 Cuts at: 3310 3427 3436 3682 3703 4236 5808 2488 Size: 117 9 246 21 533 1572 3173 Fragments arranged by size: 3173 1572 533 312 246 153 120 117 114 66 30 21 15 12 9 ApaI G_GGCC'C Cuts at: 2750 2930 3445 2750 Size: 180 515 5798 Fragments arranged by size: 5798 515 180 ApaLI G'TGCA_C Cuts at: 4550 5706 4550 Size: 1156 5337 ApoI r'AATT_y Cuts at: 1493 3802 4028 4180 6289 1493 Size: 2309 226 152 2109 1697 Fragments arranged by size: 2309 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5047 160 Size: 872 4015 1606 Fragments arranged by size: 4015 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 AvaI C'yCGr_G Cuts at: 1870 1943 2226 3050 3485 3571 3818 1870 Size: 73 283 824 435 86 247 4545 Fragments arranged by size: 4545 824 435 283 247 86 73 BamHI G'GATC_C Cuts at: 3823 4190 3823 Size: 367 6126 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2828 3317 3814 5269 618 Size: 325 217 627 1041 489 497 1455 1842 Fragments arranged by size: 1842 1455 1041 627 497 489 325 217 BanII G_rGCy'C Cuts at: 1391 2234 2354 2630 2750 2930 2937 3051 3445 Size: 843 120 276 120 180 7 114 394 Cuts at: 3445 3732 3812 1391 Size: 287 80 4072 Fragments arranged by size: 4072 843 394 287 276 180 120 120 114 80 7 BbeI G_GCGC'C Cuts at: 2832 3321 2832 Size: 489 6004 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2199 3119 928 Size: 971 300 920 4302 Fragments arranged by size: 4302 971 920 300 BbvCI CC'TCA_GC Cuts at: 2129 3296 2129 Size: 1167 5326 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2345 2379 4684 4718 1903 Size: 34 408 34 2305 34 3678 Fragments arranged by size: 3678 2305 408 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4385 5595 4385 Size: 1210 5283 BclI T'GATC_A Cuts at: 1548 1548 Size: 6493 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3455 5104 136 Size: 107 122 71 631 605 1783 1649 1525 Fragments arranged by size: 1783 1649 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3174 6488 3174 Size: 3314 3179 BlpI GC'TnA_GC Cuts at: 3910 3910 Size: 6493 Bme1580I G_kGCm'C Cuts at: 1790 2750 2930 3445 4554 5710 1790 Size: 960 180 515 1109 1156 2573 Fragments arranged by size: 2573 1156 1109 960 515 180 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6493 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3139 4224 5182 Size: 416 53 83 399 24 1722 1085 958 Cuts at: 5182 442 Size: 1753 Fragments arranged by size: 1753 1722 1085 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3234 1056 Size: 2178 4315 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2890 2890 Size: 6493 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2448 2645 2819 2822 2860 2942 3101 3155 Size: 1209 197 174 3 38 82 159 54 Cuts at: 3155 3302 3311 3332 3359 3365 3437 3536 3704 Size: 147 9 21 27 6 72 99 168 Cuts at: 3704 5153 1239 Size: 1449 2579 Fragments arranged by size: 2579 1449 1209 197 174 168 159 147 99 82 72 54 38 27 21 9 6 3 Bpu10I CC'TnA_GC Cuts at: 2129 3092 3296 2129 Size: 963 204 5326 Fragments arranged by size: 5326 963 204 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2081 2939 3233 4618 5483 5781 2081 Size: 858 294 1385 865 298 2793 Fragments arranged by size: 2793 1385 865 858 298 294 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3077 3134 3251 5156 882 Size: 617 1578 57 117 1905 2219 Fragments arranged by size: 2219 1905 1578 617 117 57 BsaAI yAC'GTr Cuts at: 493 1204 3655 3710 4284 493 Size: 711 2451 55 574 2702 Fragments arranged by size: 2702 2451 711 574 55 BsaBI GATnn'nnATC Cuts at: 6487 6487 Size: 6493 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2829 3318 3492 4300 Size: 53 83 186 936 1296 489 174 808 Cuts at: 4300 4682 5317 275 Size: 382 635 1451 Fragments arranged by size: 1451 1296 936 808 635 489 382 186 174 83 53 BsaWI w'CCGG_w Cuts at: 2109 2153 4926 5324 5598 5745 6156 2109 Size: 44 2773 398 274 147 411 2446 Fragments arranged by size: 2773 2446 411 398 274 147 44 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3131 3161 1178 Size: 30 165 30 1728 30 4510 Fragments arranged by size: 4510 1728 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2413 2873 2906 3152 3557 3620 1186 Size: 1227 460 33 246 405 63 4059 Fragments arranged by size: 4059 1227 460 405 246 63 33 BseYI C'CCAG_C Cuts at: 2285 2719 5696 6192 2285 Size: 434 2977 496 2586 Fragments arranged by size: 2977 2586 496 434 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2385 2402 2435 2806 2981 1163 Size: 573 649 17 33 371 175 4675 Fragments arranged by size: 4675 649 573 371 175 33 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2530 2900 3943 4704 5732 1540 Size: 275 186 529 370 1043 761 1028 2301 Fragments arranged by size: 2301 1043 1028 761 529 370 275 186 BsiHKAI G_wGCw'C Cuts at: 2507 3051 4554 4639 5710 2507 Size: 544 1503 85 1071 3290 Fragments arranged by size: 3290 1503 1071 544 85 BsmI GAATG_Cn' Cuts at: 4004 4097 4004 Size: 93 6400 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2234 2354 2507 2630 2750 2930 2937 Size: 399 444 120 153 123 120 180 7 Cuts at: 2937 3051 3445 3732 3812 4554 4639 5710 1391 Size: 114 394 287 80 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 444 399 394 287 180 153 123 120 120 114 85 80 7 BspEI T'CCGG_A Cuts at: 6156 6156 Size: 6493 BspHI T'CATG_A Cuts at: 3060 4382 6112 3060 Size: 1322 1730 3441 Fragments arranged by size: 3441 1730 1322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2980 2995 3009 3084 3420 3848 844 Size: 2136 15 14 75 336 428 3489 Fragments arranged by size: 3489 2136 428 336 75 15 14 BsrBI CCG'CTC Cuts at: 1394 1823 2763 2896 3520 3547 3589 4380 1394 Size: 429 940 133 624 27 42 791 3507 Fragments arranged by size: 3507 940 791 624 429 133 42 27 BsrDI GCAATG_nn' Cuts at: 65 1248 4988 5162 65 Size: 1183 3740 174 1396 Fragments arranged by size: 3740 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2109 5137 5324 1700 Size: 110 299 3028 187 2869 Fragments arranged by size: 3028 2869 299 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6493 BssHII G'CGCG_C Cuts at: 1925 2980 2982 1925 Size: 1055 2 5436 Fragments arranged by size: 5436 1055 2 BssSI C'ACGA_G Cuts at: 2752 3593 4553 5565 2752 Size: 841 960 1012 3680 Fragments arranged by size: 3680 1012 960 841 BstAPI GCAn_nnn'nTGC Cuts at: 794 2055 2995 3010 3076 3427 3436 794 Size: 1261 940 15 66 351 9 3851 Fragments arranged by size: 3851 1261 940 351 66 15 9 BstBI TT'CG_AA Cuts at: 5297 5297 Size: 6493 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6493 BstXI CCAn_nnnn'nTGG Cuts at: 3337 3337 Size: 6493 BstYI r'GATC_y Cuts at: 1972 3174 3237 3823 4190 4575 4592 6044 6130 Size: 1202 63 586 367 385 17 1452 86 Cuts at: 6130 6142 6488 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1202 586 385 367 346 86 63 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2399 2601 2775 2900 513 Size: 552 939 395 202 174 125 4106 Fragments arranged by size: 4106 939 552 395 202 174 125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5754 Fragments arranged by size: 5754 683 56 BtsI GCAGTG_nn' Cuts at: 2053 2373 4009 4803 4823 2053 Size: 320 1636 794 20 3723 Fragments arranged by size: 3723 1636 794 320 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6493 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6458 DraI TTT'AAA Cuts at: 3797 3797 Size: 6493 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2591 5500 809 Size: 507 1275 2909 1802 Fragments arranged by size: 2909 1802 1275 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2032 2527 2897 Size: 54 1425 50 275 18 202 495 370 Cuts at: 2897 3657 3940 4829 6458 8 Size: 760 283 889 1629 43 Fragments arranged by size: 1629 1425 889 760 495 370 283 275 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2527 2897 3940 1537 Size: 275 715 370 1043 4090 Fragments arranged by size: 4090 1043 715 370 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2966 4423 1353 Size: 1613 1457 3423 Fragments arranged by size: 3423 1613 1457 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2620 3401 5080 5454 5600 387 Size: 1064 138 1031 781 1679 374 146 1280 Fragments arranged by size: 1679 1280 1064 1031 781 374 146 138 EcoICRI GAG'CTC Cuts at: 3049 3049 Size: 6493 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2448 2510 2645 2705 2819 2822 2860 Size: 393 816 62 135 60 114 3 38 Cuts at: 2860 2942 3002 3101 3155 3206 3218 3281 3302 Size: 82 60 99 54 51 12 63 21 Cuts at: 3302 3311 3332 3359 3365 3437 3536 3704 4556 Size: 9 21 27 6 72 99 168 852 Cuts at: 4556 5153 5940 6208 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 852 816 787 597 393 268 168 135 114 99 99 82 72 63 62 60 60 54 51 38 27 21 21 12 9 6 3 EcoNI CCTnn'n_nnAGG Cuts at: 3062 3103 3686 3062 Size: 41 583 5869 Fragments arranged by size: 5869 583 41 EcoO109I rG'GnC_Cy Cuts at: 2606 2746 2747 2926 2992 3073 3096 3286 3300 Size: 140 1 179 66 81 23 190 14 Cuts at: 3300 3307 3334 3691 2606 Size: 7 27 357 5408 Fragments arranged by size: 5408 357 190 179 140 81 66 27 23 14 7 1 EcoRI G'AATT_C Cuts at: 3802 4180 3802 Size: 378 6115 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3136 3136 Size: 6493 FspI TGC'GCA Cuts at: 2081 3326 4999 2081 Size: 1245 1673 3575 Fragments arranged by size: 3575 1673 1245 HaeII r_GCGC'y Cuts at: 1678 2516 2832 2908 3321 3682 3723 5640 1678 Size: 838 316 76 413 361 41 1917 2531 Fragments arranged by size: 2531 1917 838 413 361 316 76 41 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3229 3261 3551 3583 5134 Size: 32 135 32 1657 32 290 32 1551 Cuts at: 5134 5166 5208 5240 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1657 1551 290 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3742 3837 4083 669 Size: 988 281 1804 95 246 3079 Fragments arranged by size: 3079 1804 988 281 246 95 HindIII A'AGCT_T Cuts at: 748 748 Size: 6493 HpaI GTT'AAC Cuts at: 4083 4083 Size: 6493 KasI G'GCGC_C Cuts at: 2828 3317 2828 Size: 489 6004 KpnI G_GTAC'C Cuts at: 3818 3818 Size: 6493 MfeI C'AATT_G Cuts at: 4092 4092 Size: 6493 MluI A'CGCG_T Cuts at: 6369 6369 Size: 6493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2142 2727 2796 5607 5791 1305 Size: 837 585 69 2811 184 2007 Fragments arranged by size: 2811 2007 837 585 184 69 MscI TGG'CCA Cuts at: 10 64 1489 1832 3659 10 Size: 54 1425 343 1827 2844 Fragments arranged by size: 2844 1827 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2600 4451 4810 4969 518 Size: 648 1434 1851 359 159 2042 Fragments arranged by size: 2042 1851 1434 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2265 2449 2581 2590 2902 3196 3259 3508 Size: 659 184 132 9 312 294 63 249 Cuts at: 3508 3640 3670 3703 3909 4586 5734 5979 1606 Size: 132 30 33 206 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 659 312 294 249 245 206 184 132 132 63 33 30 9 NarI GG'CG_CC Cuts at: 2829 3318 2829 Size: 489 6004 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5002 Fragments arranged by size: 5002 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6493 NheI G'CTAG_C Cuts at: 1052 3230 1052 Size: 2178 4315 NotI GC'GGCC_GC Cuts at: 2897 3940 2897 Size: 1043 5450 NspI r_CATG'y Cuts at: 1132 1229 3851 5396 1132 Size: 97 2622 1545 2229 Fragments arranged by size: 2622 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2647 1348 Size: 1299 5194 PciI A'CATG_T Cuts at: 5392 5392 Size: 6493 PflMI CCAn_nnn'nTGG Cuts at: 1108 2800 3310 3682 1108 Size: 1692 510 372 3919 Fragments arranged by size: 3919 1692 510 372 PfoI T'CCnGG_A Cuts at: 2661 2793 2661 Size: 132 6361 PmeI GTTT'AAAC Cuts at: 3797 3797 Size: 6493 PmlI CAC'GTG Cuts at: 3710 3710 Size: 6493 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4562 4594 6075 6107 4562 Size: 32 1481 32 4948 Fragments arranged by size: 4948 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2606 2992 3073 3096 3300 3307 2606 Size: 386 81 23 204 7 5792 Fragments arranged by size: 5792 386 204 81 23 7 PshAI GACnn'nnGTC Cuts at: 1613 2154 1613 Size: 541 5952 PsiI TTA'TAA Cuts at: 4063 4063 Size: 6493 PspOMI G'GGCC_C Cuts at: 2746 2926 3441 2746 Size: 180 515 5798 Fragments arranged by size: 5798 515 180 PstI C_TGCA'G Cuts at: 830 2847 2912 3006 3015 3369 3432 3507 3645 Size: 2017 65 94 9 354 63 75 138 Cuts at: 3645 3699 3845 830 Size: 54 146 3478 Fragments arranged by size: 3478 2017 354 146 138 94 75 65 63 54 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6493 PvuII CAG'CTG Cuts at: 2449 2581 3196 3259 3640 3703 2449 Size: 132 615 63 381 63 5239 Fragments arranged by size: 5239 615 381 132 63 63 SacI G_AGCT'C Cuts at: 3051 3051 Size: 6493 SacII CC_GC'GG Cuts at: 2903 2903 Size: 6493 SalI G'TCGA_C Cuts at: 1936 3835 1936 Size: 1899 4594 SanDI GG'GwC_CC Cuts at: 2606 2606 Size: 6493 SapI GCTCTTCn'nnn_ Cuts at: 2966 2966 Size: 6493 SbfI CC_TGCA'GG Cuts at: 3006 3845 3006 Size: 839 5654 ScaI AGT'ACT Cuts at: 4741 6453 4741 Size: 1712 4781 SfcI C'TryA_G Cuts at: 826 1045 2843 2908 3002 3011 3365 3428 3503 Size: 219 1798 65 94 9 354 63 75 Cuts at: 3503 3641 3695 3841 4976 5657 5848 826 Size: 138 54 146 1135 681 191 1471 Fragments arranged by size: 1798 1471 1135 681 354 219 191 146 138 94 75 65 63 54 9 SfiI GGCCn_nnn'nGGCC Cuts at: 3455 3455 Size: 6493 SfoI GGC'GCC Cuts at: 2830 3319 2830 Size: 489 6004 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6493 SmaI CCC'GGG Cuts at: 1872 2228 3820 1872 Size: 356 1592 4545 Fragments arranged by size: 4545 1592 356 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 2954 3050 3248 4597 5498 Size: 197 926 153 858 96 198 1349 901 Cuts at: 5498 5760 820 Size: 262 1553 Fragments arranged by size: 1553 1349 926 901 858 262 198 197 153 96 SnaBI TAC'GTA Cuts at: 493 4284 493 Size: 3791 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6493 SphI G_CATG'C Cuts at: 1132 3851 1132 Size: 2719 3774 SspI AAT'ATT Cuts at: 5 52 4417 5 Size: 47 4365 2081 Fragments arranged by size: 4365 2081 47 StuI AGG'CCT Cuts at: 1517 2963 1517 Size: 1446 5047 StyI C'CwwG_G Cuts at: 513 1065 2004 2475 3225 3330 3732 3932 513 Size: 552 939 471 750 105 402 200 3074 Fragments arranged by size: 3074 939 750 552 471 402 200 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2256 2660 4520 4537 4690 4875 5320 Size: 1255 676 404 1860 17 153 185 445 Cuts at: 5320 325 Size: 1498 Fragments arranged by size: 1860 1498 1255 676 445 404 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2070 4739 6451 Size: 274 80 33 51 1443 93 2669 1712 Cuts at: 6451 96 Size: 138 Fragments arranged by size: 2669 1712 1443 274 138 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2150 2182 3131 3163 6453 6485 2150 Size: 32 949 32 3290 32 2158 Fragments arranged by size: 3290 2158 949 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6493 XbaI T'CTAG_A Cuts at: 3829 3829 Size: 6493 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2176 2176 Size: 6493 XhoI C'TCGA_G Cuts at: 1943 3050 1943 Size: 1107 5386 XmaI C'CCGG_G Cuts at: 1870 2226 3818 1870 Size: 356 1592 4545 Fragments arranged by size: 4545 1592 356 XmnI GAAnn'nnTTC Cuts at: 4622 4622 Size: 6493 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3493 4301 276 Size: 53 83 186 936 1959 808 2468 Fragments arranged by size: 2468 1959 936 808 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SexAI SrfI SwaI