(Linear) (Six-base) MAPSORT of: KIBB6147.seq Check: 4623 from: 1 to: 1788 KIBB6147.orf With 182 enzymes: SgfI * December 27, 2011 16:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1004 1788 Size: 1004 784 AatII G_ACGT'C Cuts at: 0 1490 1788 Size: 1490 298 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 505 700 997 1201 1213 1276 1788 Size: 505 195 297 204 12 63 512 Fragments arranged by size: 512 505 297 204 195 63 12 AgeI A'CCGG_T Cuts at: 0 104 1788 Size: 104 1684 AhdI GACnn_n'nnGTC Cuts at: 0 1560 1788 Size: 1560 228 AleI CACnn'nnGTG Cuts at: 0 595 1788 Size: 595 1193 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 520 552 1788 Size: 520 32 1236 Fragments arranged by size: 1236 520 32 AlwNI CAG_nnn'CTG Cuts at: 0 483 795 807 837 990 1005 1071 1191 Size: 483 312 12 30 153 15 66 120 Cuts at: 1191 1305 1422 1431 1677 1698 1788 Size: 114 117 9 246 21 90 Fragments arranged by size: 483 312 246 153 120 117 114 90 66 30 21 15 12 9 ApaI G_GGCC'C Cuts at: 0 745 925 1440 1788 Size: 745 180 515 348 Fragments arranged by size: 745 515 348 180 AvaI C'yCGr_G Cuts at: 0 221 1045 1480 1566 1788 Size: 221 824 435 86 222 Fragments arranged by size: 824 435 222 221 86 BanI G'GyrC_C Cuts at: 0 823 1312 1788 Size: 823 489 476 Fragments arranged by size: 823 489 476 BanII G_rGCy'C Cuts at: 0 229 349 625 745 925 932 1046 1440 Size: 229 120 276 120 180 7 114 394 Cuts at: 1440 1727 1788 Size: 287 61 Fragments arranged by size: 394 287 276 229 180 120 120 114 61 7 BbeI G_GCGC'C Cuts at: 0 827 1316 1788 Size: 827 489 472 Fragments arranged by size: 827 489 472 BbsI GAAGACnn'nnnn_ Cuts at: 0 194 1114 1788 Size: 194 920 674 Fragments arranged by size: 920 674 194 BbvCI CC'TCA_GC Cuts at: 0 124 1291 1788 Size: 124 1167 497 Fragments arranged by size: 1167 497 124 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 340 374 1788 Size: 340 34 1414 Fragments arranged by size: 1414 340 34 BglI GCCn_nnn'nGGC Cuts at: 0 1450 1788 Size: 1450 338 BglII A'GATC_T Cuts at: 0 1169 1788 Size: 1169 619 Bme1580I G_kGCm'C Cuts at: 0 745 925 1440 1788 Size: 745 180 515 348 Fragments arranged by size: 745 515 348 180 BmrI ACTGGGnnnn_n' Cuts at: 0 1134 1788 Size: 1134 654 BmtI G_CTAG'C Cuts at: 0 1229 1788 Size: 1229 559 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 885 1788 Size: 885 903 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 443 640 814 817 855 937 1096 1150 Size: 443 197 174 3 38 82 159 54 Cuts at: 1150 1297 1306 1327 1354 1360 1432 1531 1699 Size: 147 9 21 27 6 72 99 168 Cuts at: 1699 1788 Size: 89 Fragments arranged by size: 443 197 174 168 159 147 99 89 82 72 54 38 27 21 9 6 3 Bpu10I CC'TnA_GC Cuts at: 0 124 1087 1291 1788 Size: 124 963 204 497 Fragments arranged by size: 963 497 204 124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 934 1228 1788 Size: 76 858 294 560 Fragments arranged by size: 858 560 294 76 BsaI GGTCTCn'nnnn_ Cuts at: 0 1072 1129 1246 1788 Size: 1072 57 117 542 Fragments arranged by size: 1072 542 117 57 BsaAI yAC'GTr Cuts at: 0 1650 1705 1788 Size: 1650 55 83 Fragments arranged by size: 1650 83 55 BsaHI Gr'CG_yC Cuts at: 0 824 1313 1487 1788 Size: 824 489 174 301 Fragments arranged by size: 824 489 301 174 BsaWI w'CCGG_w Cuts at: 0 104 148 1788 Size: 104 44 1640 Fragments arranged by size: 1640 104 44 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1126 1156 1788 Size: 1126 30 632 Fragments arranged by size: 1126 632 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 408 868 901 1147 1552 1615 1788 Size: 408 460 33 246 405 63 173 Fragments arranged by size: 460 408 405 246 173 63 33 BseYI C'CCAG_C Cuts at: 0 280 714 1788 Size: 280 434 1074 Fragments arranged by size: 1074 434 280 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 380 397 430 801 976 1788 Size: 380 17 33 371 175 812 Fragments arranged by size: 812 380 371 175 33 17 BsiEI CG_ry'CG Cuts at: 0 525 895 1788 Size: 525 370 893 Fragments arranged by size: 893 525 370 BsiHKAI G_wGCw'C Cuts at: 0 502 1046 1788 Size: 502 544 742 Fragments arranged by size: 742 544 502 Bsp1286I G_dGCh'C Cuts at: 0 229 349 502 625 745 925 932 1046 Size: 229 120 153 123 120 180 7 114 Cuts at: 1046 1440 1727 1788 Size: 394 287 61 Fragments arranged by size: 394 287 229 180 153 123 120 120 114 61 7 BspHI T'CATG_A Cuts at: 0 1055 1788 Size: 1055 733 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 975 990 1004 1079 1415 1788 Size: 975 15 14 75 336 373 Fragments arranged by size: 975 373 336 75 15 14 BsrBI CCG'CTC Cuts at: 0 758 891 1515 1542 1584 1788 Size: 758 133 624 27 42 204 Fragments arranged by size: 758 624 204 133 42 27 BsrFI r'CCGG_y Cuts at: 0 104 1788 Size: 104 1684 BssHII G'CGCG_C Cuts at: 0 975 977 1788 Size: 975 2 811 Fragments arranged by size: 975 811 2 BssSI C'ACGA_G Cuts at: 0 747 1588 1788 Size: 747 841 200 Fragments arranged by size: 841 747 200 BstAPI GCAn_nnn'nTGC Cuts at: 0 50 990 1005 1071 1422 1431 1788 Size: 50 940 15 66 351 9 357 Fragments arranged by size: 940 357 351 66 50 15 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 1332 1788 Size: 1332 456 BstYI r'GATC_y Cuts at: 0 1169 1232 1788 Size: 1169 63 556 Fragments arranged by size: 1169 556 63 BtgI C'CryG_G Cuts at: 0 394 596 770 895 1788 Size: 394 202 174 125 893 Fragments arranged by size: 893 394 202 174 125 BtsI GCAGTG_nn' Cuts at: 0 48 368 1788 Size: 48 320 1420 Fragments arranged by size: 1420 320 48 DrdI GACnn_nn'nnGTC Cuts at: 0 586 1788 Size: 586 1202 EaeI y'GGCC_r Cuts at: 0 27 522 892 1652 1788 Size: 27 495 370 760 136 Fragments arranged by size: 760 495 370 136 27 EagI C'GGCC_G Cuts at: 0 522 892 1788 Size: 522 370 896 Fragments arranged by size: 896 522 370 EarI CTCTTCn'nnn_ Cuts at: 0 961 1788 Size: 961 827 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 615 1396 1788 Size: 615 781 392 Fragments arranged by size: 781 615 392 EcoICRI GAG'CTC Cuts at: 0 1044 1788 Size: 1044 744 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 443 505 640 700 814 817 855 937 Size: 443 62 135 60 114 3 38 82 Cuts at: 937 997 1096 1150 1201 1213 1276 1297 1306 Size: 60 99 54 51 12 63 21 9 Cuts at: 1306 1327 1354 1360 1432 1531 1699 1788 Size: 21 27 6 72 99 168 89 Fragments arranged by size: 443 168 135 114 99 99 89 82 72 63 62 60 60 54 51 38 27 21 21 12 9 6 3 EcoNI CCTnn'n_nnAGG Cuts at: 0 1057 1098 1681 1788 Size: 1057 41 583 107 Fragments arranged by size: 1057 583 107 41 EcoO109I rG'GnC_Cy Cuts at: 0 601 741 742 921 987 1068 1091 1281 Size: 601 140 1 179 66 81 23 190 Cuts at: 1281 1295 1302 1329 1686 1788 Size: 14 7 27 357 102 Fragments arranged by size: 601 357 190 179 140 102 81 66 27 23 14 7 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1131 1788 Size: 1131 657 FspI TGC'GCA Cuts at: 0 76 1321 1788 Size: 76 1245 467 Fragments arranged by size: 1245 467 76 HaeII r_GCGC'y Cuts at: 0 511 827 903 1316 1677 1718 1788 Size: 511 316 76 413 361 41 70 Fragments arranged by size: 511 413 361 316 76 70 41 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1224 1256 1546 1578 1788 Size: 1224 32 290 32 210 Fragments arranged by size: 1224 290 210 32 32 HincII GTy'rAC Cuts at: 0 1737 1788 Size: 1737 51 KasI G'GCGC_C Cuts at: 0 823 1312 1788 Size: 823 489 476 Fragments arranged by size: 823 489 476 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 137 722 791 1788 Size: 137 585 69 997 Fragments arranged by size: 997 585 137 69 MscI TGG'CCA Cuts at: 0 1654 1788 Size: 1654 134 MslI CAynn'nnrTG Cuts at: 0 595 1788 Size: 595 1193 MspA1I CmG'CkG Cuts at: 0 260 444 576 585 897 1191 1254 1503 Size: 260 184 132 9 312 294 63 249 Cuts at: 1503 1635 1665 1698 1788 Size: 132 30 33 90 Fragments arranged by size: 312 294 260 249 184 132 132 90 63 33 30 9 NarI GG'CG_CC Cuts at: 0 824 1313 1788 Size: 824 489 475 Fragments arranged by size: 824 489 475 NheI G'CTAG_C Cuts at: 0 1225 1788 Size: 1225 563 NotI GC'GGCC_GC Cuts at: 0 892 1788 Size: 892 896 PasI CC'CwG_GG Cuts at: 0 642 1788 Size: 642 1146 PflMI CCAn_nnn'nTGG Cuts at: 0 795 1305 1677 1788 Size: 795 510 372 111 Fragments arranged by size: 795 510 372 111 PfoI T'CCnGG_A Cuts at: 0 656 788 1788 Size: 656 132 1000 Fragments arranged by size: 1000 656 132 PmlI CAC'GTG Cuts at: 0 1705 1788 Size: 1705 83 PpuMI rG'GwC_Cy Cuts at: 0 601 987 1068 1091 1295 1302 1788 Size: 601 386 81 23 204 7 486 Fragments arranged by size: 601 486 386 204 81 23 7 PshAI GACnn'nnGTC Cuts at: 0 149 1788 Size: 149 1639 PspOMI G'GGCC_C Cuts at: 0 741 921 1436 1788 Size: 741 180 515 352 Fragments arranged by size: 741 515 352 180 PstI C_TGCA'G Cuts at: 0 842 907 1001 1010 1364 1427 1502 1640 Size: 842 65 94 9 354 63 75 138 Cuts at: 1640 1694 1788 Size: 54 94 Fragments arranged by size: 842 354 138 94 94 75 65 63 54 9 PvuII CAG'CTG Cuts at: 0 444 576 1191 1254 1635 1698 1788 Size: 444 132 615 63 381 63 90 Fragments arranged by size: 615 444 381 132 90 63 63 SacI G_AGCT'C Cuts at: 0 1046 1788 Size: 1046 742 SacII CC_GC'GG Cuts at: 0 898 1788 Size: 898 890 SanDI GG'GwC_CC Cuts at: 0 601 1788 Size: 601 1187 SapI GCTCTTCn'nnn_ Cuts at: 0 961 1788 Size: 961 827 SbfI CC_TGCA'GG Cuts at: 0 1001 1788 Size: 1001 787 SfcI C'TryA_G Cuts at: 0 838 903 997 1006 1360 1423 1498 1636 Size: 838 65 94 9 354 63 75 138 Cuts at: 1636 1690 1788 Size: 54 98 Fragments arranged by size: 838 354 138 98 94 75 65 63 54 9 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1450 1788 Size: 1450 338 SfoI GGC'GCC Cuts at: 0 825 1314 1788 Size: 825 489 474 Fragments arranged by size: 825 489 474 SmaI CCC'GGG Cuts at: 0 223 1788 Size: 223 1565 SmlI C'TyrA_G Cuts at: 0 91 949 1045 1243 1788 Size: 91 858 96 198 545 Fragments arranged by size: 858 545 198 96 91 StuI AGG'CCT Cuts at: 0 958 1788 Size: 958 830 StyI C'CwwG_G Cuts at: 0 470 1220 1325 1727 1788 Size: 470 750 105 402 61 Fragments arranged by size: 750 470 402 105 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 251 655 1788 Size: 251 404 1133 Fragments arranged by size: 1133 404 251 TatI w'GTAC_w Cuts at: 0 65 1788 Size: 65 1723 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 145 177 1126 1158 1788 Size: 145 32 949 32 630 Fragments arranged by size: 949 630 145 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 171 1788 Size: 171 1617 XhoI C'TCGA_G Cuts at: 0 1045 1788 Size: 1045 743 XmaI C'CCGG_G Cuts at: 0 221 1788 Size: 221 1567 ZraI GAC'GTC Cuts at: 0 1488 1788 Size: 1488 300 Enzymes that do cut: AarI AatII AcuI AgeI AhdI AleI AloI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NheI NotI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI SapI SbfI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XhoI XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BlpI BmgBI BsaBI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NruI NsiI NspI PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SalI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmnI