(Circular) (Six-base) MAPSORT of: pFN21AE1706.seq Check: 9219 from: 1 to: 5338 pFN21AE1706 5338 bp With 182 enzymes: SgfI * June 19, 2019 18:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2053 2190 2241 2053 Size: 137 51 5150 Fragments arranged by size: 5150 137 51 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3148 278 Size: 53 83 186 936 1612 2468 Fragments arranged by size: 2468 1612 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2681 1937 Size: 744 4594 Acc65I G'GTAC_C Cuts at: 2659 2659 Size: 5338 AclI AA'CG_TT Cuts at: 1563 3465 3838 1563 Size: 1902 373 3063 Fragments arranged by size: 3063 1902 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3401 4785 5053 1632 Size: 1769 1384 268 1917 Fragments arranged by size: 1917 1769 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5141 AflIII A'CryG_T Cuts at: 1203 4237 5214 1203 Size: 3034 977 1327 Fragments arranged by size: 3034 1327 977 AgeI A'CCGG_T Cuts at: 4169 4169 Size: 5338 AhdI GACnn_n'nnGTC Cuts at: 4067 4067 Size: 5338 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5338 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5338 AlwNI CAG_nnn'CTG Cuts at: 2242 3081 4653 2242 Size: 839 1572 2927 Fragments arranged by size: 2927 1572 839 ApaLI G'TGCA_C Cuts at: 3395 4551 3395 Size: 1156 4182 ApoI r'AATT_y Cuts at: 1493 2300 2561 2647 2873 3025 5134 1493 Size: 807 261 86 226 152 2109 1697 Fragments arranged by size: 2109 1697 807 261 226 152 86 AseI AT'TA_AT Cuts at: 160 1032 3892 160 Size: 872 2860 1606 Fragments arranged by size: 2860 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 AvaI C'yCGr_G Cuts at: 1870 1943 2663 1870 Size: 73 720 4545 Fragments arranged by size: 4545 720 73 BamHI G'GATC_C Cuts at: 2590 2668 3035 2590 Size: 78 367 4893 Fragments arranged by size: 4893 367 78 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2264 2537 2659 4114 618 Size: 325 217 627 477 273 122 1455 1842 Fragments arranged by size: 1842 1455 627 477 325 273 217 122 BanII G_rGCy'C Cuts at: 1391 2657 1391 Size: 1266 4072 BbeI G_GCGC'C Cuts at: 2541 2541 Size: 5338 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4367 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3529 3563 1903 Size: 34 1592 34 3678 Fragments arranged by size: 3678 1592 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3230 4440 3230 Size: 1210 4128 BclI T'GATC_A Cuts at: 1548 1548 Size: 5338 BfrBI ATG'CAT Cuts at: 2415 2415 Size: 5338 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2049 2223 3949 Size: 107 122 71 631 605 377 174 1726 Cuts at: 3949 136 Size: 1525 Fragments arranged by size: 1726 1525 631 605 377 174 122 107 71 BglII A'GATC_T Cuts at: 5333 5333 Size: 5338 BlpI GC'TnA_GC Cuts at: 2755 2755 Size: 5338 Bme1580I G_kGCm'C Cuts at: 1790 2267 3399 4555 1790 Size: 477 1132 1156 2573 Fragments arranged by size: 2573 1156 1132 477 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5338 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3069 4027 442 Size: 416 53 83 399 24 1652 958 1753 Fragments arranged by size: 1753 1652 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 3998 1239 Size: 827 1932 2579 Fragments arranged by size: 2579 1932 827 Bpu10I CC'TnA_GC Cuts at: 2014 2014 Size: 5338 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3463 4328 4626 3463 Size: 865 298 4175 Fragments arranged by size: 4175 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2357 4001 882 Size: 617 858 1644 2219 Fragments arranged by size: 2219 1644 858 617 BsaAI yAC'GTr Cuts at: 493 1204 3129 493 Size: 711 1925 2702 Fragments arranged by size: 2702 1925 711 BsaBI GATnn'nnATC Cuts at: 5332 5332 Size: 5338 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2538 3145 3527 4162 Size: 53 83 186 936 1005 607 382 635 Cuts at: 4162 275 Size: 1451 Fragments arranged by size: 1451 1005 936 635 607 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3771 4169 4443 4590 5001 3771 Size: 398 274 147 411 4108 Fragments arranged by size: 4108 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5113 Fragments arranged by size: 5113 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5338 BseYI C'CCAG_C Cuts at: 2113 4541 5037 2113 Size: 2428 496 2414 Fragments arranged by size: 2428 2414 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2168 2279 1163 Size: 573 432 111 4222 Fragments arranged by size: 4222 573 432 111 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2105 2788 3549 4577 1540 Size: 275 186 104 683 761 1028 2301 Fragments arranged by size: 2301 1028 761 683 275 186 104 BsiHKAI G_wGCw'C Cuts at: 3399 3484 4555 3399 Size: 85 1071 4182 Fragments arranged by size: 4182 1071 85 BsmI GAATG_Cn' Cuts at: 2417 2849 2942 2417 Size: 432 93 4813 Fragments arranged by size: 4813 432 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2267 2657 3399 3484 4555 1391 Size: 399 477 390 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 477 399 390 85 BspEI T'CCGG_A Cuts at: 5001 5001 Size: 5338 BspHI T'CATG_A Cuts at: 3227 4957 3227 Size: 1730 3608 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2053 2190 2241 2484 2693 844 Size: 1209 137 51 243 209 3489 Fragments arranged by size: 3489 1209 243 209 137 51 BsrBI CCG'CTC Cuts at: 1394 1823 2101 2140 3225 1394 Size: 429 278 39 1085 3507 Fragments arranged by size: 3507 1085 429 278 39 BsrDI GCAATG_nn' Cuts at: 65 1248 3833 4007 65 Size: 1183 2585 174 1396 Fragments arranged by size: 2585 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2202 2222 3982 4169 1700 Size: 110 392 20 1760 187 2869 Fragments arranged by size: 2869 1760 392 187 110 20 BsrGI T'GTAC_A Cuts at: 96 2613 2631 96 Size: 2517 18 2803 Fragments arranged by size: 2803 2517 18 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5338 BssSI C'ACGA_G Cuts at: 3398 4410 3398 Size: 1012 4326 BstAPI GCAn_nnn'nTGC Cuts at: 794 2242 794 Size: 1448 3890 BstBI TT'CG_AA Cuts at: 4142 4142 Size: 5338 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5338 BstYI r'GATC_y Cuts at: 1972 2238 2590 2668 3035 3420 3437 4889 4975 Size: 266 352 78 367 385 17 1452 86 Cuts at: 4975 4987 5333 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 352 346 266 86 78 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2149 513 Size: 552 939 145 3702 Fragments arranged by size: 3702 939 552 145 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4599 Fragments arranged by size: 4599 683 56 BtsI GCAGTG_nn' Cuts at: 2322 2854 3648 3668 2322 Size: 532 794 20 3992 Fragments arranged by size: 3992 794 532 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5338 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5303 DraI TTT'AAA Cuts at: 2642 2642 Size: 5338 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4345 809 Size: 507 3029 1802 Fragments arranged by size: 3029 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2102 2488 2785 Size: 54 1425 50 275 18 272 386 297 Cuts at: 2785 3674 5303 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 386 297 275 272 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2102 2785 1537 Size: 275 290 683 4090 Fragments arranged by size: 4090 683 290 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2291 2430 3268 1353 Size: 938 139 838 3423 Fragments arranged by size: 3423 938 838 139 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2150 3925 4299 4445 387 Size: 1064 138 561 1775 374 146 1280 Fragments arranged by size: 1775 1280 1064 561 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 3401 3998 4785 5053 1239 Size: 393 434 1335 597 787 268 1524 Fragments arranged by size: 1524 1335 787 597 434 393 268 EcoO109I rG'GnC_Cy Cuts at: 2620 2620 Size: 5338 EcoRI G'AATT_C Cuts at: 2300 2647 3025 2300 Size: 347 378 4613 Fragments arranged by size: 4613 378 347 FspI TGC'GCA Cuts at: 2169 3844 2169 Size: 1675 3663 HaeII r_GCGC'y Cuts at: 1678 2148 2541 4485 1678 Size: 470 393 1944 2531 Fragments arranged by size: 2531 1944 470 393 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3979 4011 4053 4085 1373 Size: 32 135 32 2407 32 42 32 2626 Fragments arranged by size: 2626 2407 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2682 2928 669 Size: 988 281 744 246 3079 Fragments arranged by size: 3079 988 744 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5338 HpaI GTT'AAC Cuts at: 2928 2928 Size: 5338 KasI G'GCGC_C Cuts at: 2537 2537 Size: 5338 KpnI G_GTAC'C Cuts at: 2663 2663 Size: 5338 MfeI C'AATT_G Cuts at: 2937 2937 Size: 5338 MluI A'CGCG_T Cuts at: 5214 5214 Size: 5338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4452 4636 1305 Size: 3147 184 2007 Fragments arranged by size: 3147 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3516 Fragments arranged by size: 3516 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2309 3296 3655 3814 518 Size: 648 1143 987 359 159 2042 Fragments arranged by size: 2042 1143 987 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2110 2151 2173 2754 3431 4579 4824 1606 Size: 504 41 22 581 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 581 504 245 41 22 NaeI GCC'GGC Cuts at: 2204 2204 Size: 5338 NarI GG'CG_CC Cuts at: 2538 2538 Size: 5338 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3847 Fragments arranged by size: 3847 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5338 NgoMIV G'CCGG_C Cuts at: 2202 2202 Size: 5338 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5338 NotI GC'GGCC_GC Cuts at: 2102 2785 2102 Size: 683 4655 NsiI A_TGCA'T Cuts at: 2417 2417 Size: 5338 NspI r_CATG'y Cuts at: 1132 1229 2696 4241 1132 Size: 97 1467 1545 2229 Fragments arranged by size: 2229 1545 1467 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5338 PciI A'CATG_T Cuts at: 4237 4237 Size: 5338 PflMI CCAn_nnn'nTGG Cuts at: 1108 2065 2534 1108 Size: 957 469 3912 Fragments arranged by size: 3912 957 469 PmeI GTTT'AAAC Cuts at: 2642 2642 Size: 5338 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3407 3439 4920 4952 3407 Size: 32 1481 32 3793 Fragments arranged by size: 3793 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2620 2620 Size: 5338 PshAI GACnn'nnGTC Cuts at: 1613 2349 1613 Size: 736 4602 PsiI TTA'TAA Cuts at: 2908 2908 Size: 5338 PstI C_TGCA'G Cuts at: 830 2238 2690 830 Size: 1408 452 3478 Fragments arranged by size: 3478 1408 452 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5338 PvuII CAG'CTG Cuts at: 2173 2173 Size: 5338 SacII CC_GC'GG Cuts at: 2152 2152 Size: 5338 SalI G'TCGA_C Cuts at: 1936 2680 1936 Size: 744 4594 SbfI CC_TGCA'GG Cuts at: 2690 2690 Size: 5338 ScaI AGT'ACT Cuts at: 3586 5298 3586 Size: 1712 3626 SfcI C'TryA_G Cuts at: 826 1045 2234 2686 3821 4502 4693 826 Size: 219 1189 452 1135 681 191 1471 Fragments arranged by size: 1471 1189 1135 681 452 219 191 SfoI GGC'GCC Cuts at: 2539 2539 Size: 5338 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5338 SmaI CCC'GGG Cuts at: 1872 2665 1872 Size: 793 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3442 4343 4605 820 Size: 197 926 1499 901 262 1553 Fragments arranged by size: 1553 1499 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3129 493 Size: 2636 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5338 SphI G_CATG'C Cuts at: 1132 2696 1132 Size: 1564 3774 SspI AAT'ATT Cuts at: 5 52 3262 5 Size: 47 3210 2081 Fragments arranged by size: 3210 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5338 StyI C'CwwG_G Cuts at: 513 1065 2004 2777 513 Size: 552 939 773 3074 Fragments arranged by size: 3074 939 773 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3365 3382 3535 3720 4165 325 Size: 1255 1785 17 153 185 445 1498 Fragments arranged by size: 1785 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2613 2631 3584 Size: 274 80 33 51 1443 636 18 953 Cuts at: 3584 5296 96 Size: 1712 138 Fragments arranged by size: 1712 1443 953 636 274 138 80 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5298 5330 5298 Size: 32 5306 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5338 XbaI T'CTAG_A Cuts at: 2674 2674 Size: 5338 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5338 XmaI C'CCGG_G Cuts at: 1870 2663 1870 Size: 793 4545 XmnI GAAnn'nnTTC Cuts at: 2601 3467 2601 Size: 866 4472 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3146 276 Size: 53 83 186 936 1612 2468 Fragments arranged by size: 2468 1612 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI