Restriction map for
pFN21AE1706
(Linear) (Six-base) MAPSORT of: KIEE1706.seq Check: 7232 from: 1 to: 633
KIEE1706.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 48 185 236 633
Size: 48 137 51 397
Fragments arranged by size:
397 137 51 48
AlwNI CAG_nnn'CTG
Cuts at: 0 237 633
Size: 237 396
ApoI r'AATT_y
Cuts at: 0 295 556 633
Size: 295 261 77
Fragments arranged by size:
295 261 77
BamHI G'GATC_C
Cuts at: 0 585 633
Size: 585 48
BanI G'GyrC_C
Cuts at: 0 259 532 633
Size: 259 273 101
Fragments arranged by size:
273 259 101
BbeI G_GCGC'C
Cuts at: 0 536 633
Size: 536 97
BfrBI ATG'CAT
Cuts at: 0 410 633
Size: 410 223
BglI GCCn_nnn'nGGC
Cuts at: 0 44 218 633
Size: 44 174 415
Fragments arranged by size:
415 174 44
Bme1580I G_kGCm'C
Cuts at: 0 262 633
Size: 262 371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 633
Size: 61 572
Bpu10I CC'TnA_GC
Cuts at: 0 9 633
Size: 9 624
BsaI GGTCTCn'nnnn_
Cuts at: 0 352 633
Size: 352 281
BsaHI Gr'CG_yC
Cuts at: 0 533 633
Size: 533 100
BseYI C'CCAG_C
Cuts at: 0 108 633
Size: 108 525
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 274 633
Size: 163 111 359
Fragments arranged by size:
359 163 111
BsiEI CG_ry'CG
Cuts at: 0 100 633
Size: 100 533
BsmI GAATG_Cn'
Cuts at: 0 412 633
Size: 412 221
Bsp1286I G_dGCh'C
Cuts at: 0 262 633
Size: 262 371
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 48 185 236 479 633
Size: 48 137 51 243 154
Fragments arranged by size:
243 154 137 51 48
BsrBI CCG'CTC
Cuts at: 0 96 135 633
Size: 96 39 498
Fragments arranged by size:
498 96 39
BsrFI r'CCGG_y
Cuts at: 0 197 217 633
Size: 197 20 416
Fragments arranged by size:
416 197 20
BsrGI T'GTAC_A
Cuts at: 0 608 626 633
Size: 608 18 7
Fragments arranged by size:
608 18 7
BstAPI GCAn_nnn'nTGC
Cuts at: 0 237 633
Size: 237 396
BstYI r'GATC_y
Cuts at: 0 233 585 633
Size: 233 352 48
Fragments arranged by size:
352 233 48
BtgI C'CryG_G
Cuts at: 0 144 633
Size: 144 489
BtsI GCAGTG_nn'
Cuts at: 0 317 633
Size: 317 316
EaeI y'GGCC_r
Cuts at: 0 97 483 633
Size: 97 386 150
Fragments arranged by size:
386 150 97
EagI C'GGCC_G
Cuts at: 0 97 633
Size: 97 536
EarI CTCTTCn'nnn_
Cuts at: 0 286 425 633
Size: 286 139 208
Fragments arranged by size:
286 208 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 145 633
Size: 145 488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 633
Size: 61 572
EcoO109I rG'GnC_Cy
Cuts at: 0 615 633
Size: 615 18
EcoRI G'AATT_C
Cuts at: 0 295 633
Size: 295 338
FspI TGC'GCA
Cuts at: 0 164 633
Size: 164 469
HaeII r_GCGC'y
Cuts at: 0 143 536 633
Size: 143 393 97
Fragments arranged by size:
393 143 97
KasI G'GCGC_C
Cuts at: 0 532 633
Size: 532 101
MslI CAynn'nnrTG
Cuts at: 0 304 633
Size: 304 329
MspA1I CmG'CkG
Cuts at: 0 105 146 168 633
Size: 105 41 22 465
Fragments arranged by size:
465 105 41 22
NaeI GCC'GGC
Cuts at: 0 199 633
Size: 199 434
NarI GG'CG_CC
Cuts at: 0 533 633
Size: 533 100
NgoMIV G'CCGG_C
Cuts at: 0 197 633
Size: 197 436
NotI GC'GGCC_GC
Cuts at: 0 97 633
Size: 97 536
NsiI A_TGCA'T
Cuts at: 0 412 633
Size: 412 221
PflMI CCAn_nnn'nTGG
Cuts at: 0 60 529 633
Size: 60 469 104
Fragments arranged by size:
469 104 60
PpuMI rG'GwC_Cy
Cuts at: 0 615 633
Size: 615 18
PshAI GACnn'nnGTC
Cuts at: 0 344 633
Size: 344 289
PstI C_TGCA'G
Cuts at: 0 233 633
Size: 233 400
PvuII CAG'CTG
Cuts at: 0 168 633
Size: 168 465
SacII CC_GC'GG
Cuts at: 0 147 633
Size: 147 486
SfcI C'TryA_G
Cuts at: 0 229 633
Size: 229 404
SfoI GGC'GCC
Cuts at: 0 534 633
Size: 534 99
TatI w'GTAC_w
Cuts at: 0 608 626 633
Size: 608 18 7
Fragments arranged by size:
608 18 7
XmnI GAAnn'nnTTC
Cuts at: 0 596 633
Size: 596 37
Enzymes that do cut:
AarI AlwNI ApoI BamHI BanI BbeI BfrBI BglI
Bme1580I BpmI Bpu10I BsaI BsaHI BseYI BsgI BsiEI
BsmI Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI BstYI
BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII KasI MslI MspA1I NaeI NarI
NgoMIV NotI NsiI PflMI PpuMI PshAI PstI PvuII
SacII SfcI SfoI TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanII BbsI BbvCI
BcgI BciVI BclI BglII BlpI BmgBI BmrI BmtI
BplI BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrDI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoNI EcoRV FalI FseI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NcoI NdeI NheI NruI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI