################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstUI BstXI BstYI Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT14I EcoT22I FauI FseI FspI HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 397 GTATAC GT^[AC][GT]AC AcsI 700 AAATTT [GA]^AATT[CT] AlwI 939 GATCC GGATC(4/5) AlwNI 544 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 700 AAATTT [GA]^AATT[CT] AseI 712 ATTAAT AT^TAAT AsnI 712 ATTAAT AT^TAAT AvaII 861 GGTCC G^G[AT]CC BanI 955 GGTGCC G^G[CT][GA]CC BbsI 37 GAAGAC GAAGAC(2/6) BbvCI 856 GCTGAGG CCTCAGC(-5/-2) BclI 407 TGATCA T^GATCA BfaI 745 CTAG C^TAG BpuAI 37 GAAGAC GAAGAC(2/6) BsaI 829 GAGACC GGTCTC(1/5) BsaJI 761 CCCAGG C^C[ACGT][ACGT]GG BspMI 952 GCAGGT ACCTGC(4/8) BsrDI 582 CATTGC GCAATG(2/0) Bst1107I 397 GTATAC GTA^TAC BstEII 756 GGTGACC G^GT[ACGT]ACC BstPI 756 GGTGACC G^GT[ACGT]ACC BstZ17I 397 GTATAC GTA^TAC CfoI 187 GCGC GCG^C DraII 764 AGGCCCT [GA]G^G[ACGT]CC[CT] EarI 385 CTCTTC CTCTTC(1/4) EcoO109I 764 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 756 GGTGACC G^GT[ACGT]ACC EcoRV 748 GATATC GAT^ATC FbaI 407 TGATCA T^GATCA HaeII 186 AGCGCC [GA]GCGC^[CT] HhaI 187 GCGC GCG^C HinP1I 187 GCGC G^CGC HindIII 112 AAGCTT A^AGCTT Ksp632I 385 CTCTTC CTCTTC(1/4) MaeI 745 CTAG C^TAG MaeII 571 ACGT A^CGT NlaIV 955 GGTGCC GG[ACGT]^[ACGT]CC NspI 59 ACATGC [GA]CATG^[CT] PflMI 864 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 712 ATTAAT AT^TAAT SmlI 490 CTCAAG C^T[CT][GA]AG StuI 149 AGGCCT AGG^CCT Van91I 864 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AfaI 283 GTAC GT^AC 573 GTAC BbvI 184 GCAGC GCAGC(8/12) 721 GCTGC BsiYI 445 CCTATCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 864 CCATCGGCTGG BslI 445 CCTATCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 864 CCATCGGCTGG BsmAI 829 GAGAC GTCTC(1/5) 970 GAGAC BsmI 224 GAATGC GAATGC(1/-1) 456 GAATGC BspHI 68 TCATGA T^CATGA 536 TCATGA BsrI 825 ACTGG ACTGG(1/-1) 104 CCAGT BssKI 549 CCTGG ^CC[ACGT]GG 762 CCAGG BstNI 549 CCTGG CC^[AT]GG 762 CCAGG Cfr13I 765 GGCCC G^G[ACGT]CC 861 GGTCC DpnII 408 GATC ^GATC 939 GATC EcoRII 549 CCTGG ^CC[AT]GG 762 CCAGG Fnu4HI 184 GCAGC GC^[ACGT]GC 721 GCTGC HaeIII 150 GGCC GG^CC 765 GGCC HphI 756 GGTGA GGTGA(8/7) 274 TCACC ItaI 184 GCAGC GC^[ACGT]GC 721 GCTGC MaeIII 75 GTCAC ^GT[ACGT]AC 757 GTGAC MboI 408 GATC ^GATC 939 GATC MboII 37 GAAGA GAAGA(8/7) 386 TCTTC MslI 77 CACACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 627 CATATTTGTG MvaI 549 CCTGG CC^[AT]GG 762 CCAGG NdeII 408 GATC ^GATC 939 GATC PleI 40 GACTC GAGTC(4/5) 670 GAGTC RcaI 68 TCATGA T^CATGA 536 TCATGA RsaI 283 GTAC GT^AC 573 GTAC Sau3AI 408 GATC ^GATC 939 GATC Sau96I 765 GGCCC G^G[ACGT]CC 861 GGTCC ScrFI 549 CCTGG CC^[ACGT]GG 762 CCAGG SfaNI 376 GATGC GCATC(5/9) 391 GATGC TaqI 20 TCGA T^CGA 389 TCGA TfiI 65 GATTC G^A[AT]TC 485 GAATC TseI 184 GCAGC G^C[AT]GC 721 GCTGC Tsp45I 75 GTCAC ^GT[GC]AC 757 GTGAC TspRI 191 CACTG CA[GC]TG[ACGT][ACGT]^ 450 CAGTG TthHB8I 20 TCGA T^CGA 389 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AluI BpmI BstF5I Cac8I DdeI FokI HinfI MnlI MseI MwoI NlaIII Tru9I Tsp509I