################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaII AviII AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AlwI 681 GATCC GGATC(4/5) AocI 873 CCTAAGG CC^T[ACGT]AGG AvaI 68 CTCGGG C^[CT]CG[GA]G BbrPI 717 CACGTG CAC^GTG BbvCI 235 CCTCAGC CCTCAGC(-5/-2) BpmI 789 CTCCAG CTGGAG(16/14) BsaAI 717 CACGTG [CT]AC^GT[GA] BsaI 72 GGTCTC GGTCTC(1/5) BsoBI 68 CTCGGG C^[CT]CG[GA]G BstYI 680 AGATCC [GA]^GATC[CT] Bsu36I 873 CCTAAGG CC^T[ACGT]AGG Cfr13I 896 GGGCC G^G[ACGT]CC DraII 895 GGGGCCT [GA]G^G[ACGT]CC[CT] DrdI 64 GACACTCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 873 CCTAAGG CC^T[ACGT]AGG EcoO109I 895 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 317 CCTTGG C^C[AT][AT]GG EcoT22I 406 ATGCAT ATGCA^T HincII 930 GTTAAC GT[CT]^[GA]AC HindII 930 GTTAAC GT[CT]^[GA]AC HindIII 707 AAGCTT A^AGCTT HinfI 161 GACTC G^A[ACGT]TC HpaI 930 GTTAAC GTT^AAC MaeII 718 ACGT A^CGT MflI 680 AGATCC [GA]^GATC[CT] NlaIV 895 GGGGCC GG[ACGT]^[ACGT]CC NsiI 406 ATGCAT ATGCA^T PflMI 53 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 161 GACTC GAGTC(4/5) PmaCI 717 CACGTG CAC^GTG PmlI 717 CACGTG CAC^GTG Sau96I 896 GGGCC G^G[ACGT]CC SfaNI 382 GATGC GCATC(5/9) StyI 317 CCTTGG C^C[AT][AT]GG TaqI 206 TCGA T^CGA TthHB8I 206 TCGA T^CGA Van91I 53 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 680 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 346 GCGG AcsI 31 GAATTT [GA]^AATT[CT] 919 AAATTC AflIII 716 ACACGT A^C[GA][CT]GT 718 ACGTGT AlwNI 54 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG 238 CAGCTGCTG ApoI 31 GAATTT [GA]^AATT[CT] 919 AAATTC BalI 431 TGGCCA TGG^CCA 761 TGGCCA BclI 143 TGATCA T^GATCA 522 TGATCA BseRI 40 CTCCTC GAGGAG(10/8) 576 CTCCTC BstEII 108 GGTGACC G^GT[ACGT]ACC 588 GGTCACC BstPI 108 GGTGACC G^GT[ACGT]ACC 588 GGTCACC DdeI 236 CTCAG C^T[ACGT]AG 874 CTAAG EcoO65I 108 GGTGACC G^GT[ACGT]ACC 588 GGTCACC FbaI 143 TGATCA T^GATCA 522 TGATCA MboII 698 GAAGA GAAGA(8/7) 615 TCTTC MluNI 431 TGGCCA TGG^CCA 761 TGGCCA MscI 431 TGGCCA TGG^CCA 761 TGGCCA MseI 841 TTAA T^TAA 931 TTAA MspA1I 238 CAGCTG C[AC]G^C[GT]G 493 CAGCTG PvuII 238 CAGCTG CAG^CTG 493 CAGCTG Tru9I 841 TTAA T^TAA 931 TTAA Tsp509I 32 AATT ^AATT 920 AATT TspRI 54 CAGTG CA[GC]TG[ACGT][ACGT]^ 450 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AluI Asp700I BbvI BfaI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I DpnII EaeI EcoRII Fnu4HI FokI HaeIII HphI ItaI MaeI MaeIII MboI MnlI MslI MvaI MwoI NdeII NlaIII Sau3AI ScrFI TseI Tsp45I XmnI