################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MaeII MamI MluI MroI MslI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 563 GGTACC G^GTACC AciI 434 CCGC CCGC(-3/-1) Alw44I 407 GTGCAC G^TGCAC AlwNI 637 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 407 GTGCAC G^TGCAC Asp718I 563 GGTACC G^GTACC AspHI 407 GTGCAC G[AT]GC[AT]^C AvaII 934 GGACC G^G[AT]CC BalI 834 TGGCCA TGG^CCA BamHI 925 GGATCC G^GATCC BanI 563 GGTACC G^G[CT][GA]CC BclI 443 TGATCA T^GATCA BfaI 380 CTAG C^TAG BmyI 407 GTGCAC G[AGT]GC[ACT]^C BpmI 660 CTCCAG CTGGAG(16/14) BsaWI 896 TCCGGT [AT]^CCGG[AT] BseRI 522 GAGGAG GAGGAG(10/8) BsiHKAI 407 GTGCAC G[AT]GC[AT]^C Bsp1286I 407 GTGCAC G[AGT]GC[ACT]^C BspMI 566 ACCTGC ACCTGC(4/8) BstAPI 636 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 543 CCATGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 933 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 834 TGGCCA [CT]^GGCC[GA] EcoO109I 933 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 929 CCAAGG C^C[AT][AT]GG FbaI 443 TGATCA T^GATCA HapII 897 CCGG C^CGG HpaII 897 CCGG C^CGG KpnI 563 GGTACC GGTAC^C MaeI 380 CTAG C^TAG MaeIII 499 GTGAC ^GT[ACGT]AC MfeI 69 CAATTG C^AATTG MluNI 834 TGGCCA TGG^CCA MscI 834 TGGCCA TGG^CCA MspA1I 99 CAGCTG C[AC]G^C[GT]G MspI 897 CCGG C^CGG MunI 69 CAATTG C^AATTG PpuMI 933 GGGACCC [GA]G^G[AT]CC[CT] PvuII 99 CAGCTG CAG^CTG StyI 929 CCAAGG C^C[AT][AT]GG Tsp45I 499 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 59 GAATTC [GA]^AATT[CT] 208 GAATTC AfaI 431 GTAC GT^AC 564 GTAC ApoI 59 GAATTC [GA]^AATT[CT] 208 GAATTC BbvI 98 GCAGC GCAGC(8/12) 642 GCTGC BsrI 54 ACTGG ACTGG(1/-1) 335 CCAGT BstF5I 705 CATCC GGATG(2/0) 395 GGATG BstYI 666 AGATCC [GA]^GATC[CT] 925 GGATCC Cac8I 294 GCTTGC GC[ACGT]^[ACGT]GC 832 GCTGGC EarI 724 CTCTTC CTCTTC(1/4) 519 GAAGAG EcoRI 59 GAATTC G^AATTC 208 GAATTC Fnu4HI 98 GCAGC GC^[ACGT]GC 642 GCTGC FokI 705 CATCC GGATG(9/13) 395 GGATG ItaI 98 GCAGC GC^[ACGT]GC 642 GCTGC Ksp632I 724 CTCTTC CTCTTC(1/4) 519 GAAGAG MflI 666 AGATCC [GA]^GATC[CT] 925 GGATCC MwoI 627 GCACACACGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 636 GCAGATGCTGC NlaIII 226 CATG CATG^ 544 CATG RsaI 431 GTAC GT^AC 564 GTAC SmlI 159 CTTGAG C^T[CT][GA]AG 364 CTCAAG TseI 98 GCAGC G^C[AT]GC 642 GCTGC XhoII 666 AGATCC [GA]^GATC[CT] 925 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AluI AlwI BsaJI BsiYI BslI BsmFI BssKI BstNI Cfr13I DdeI DpnII EcoRII HaeIII HinfI HphI MboI MboII MnlI MseI MvaI NdeII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I Tsp509I TspRI