(Circular) (Six-base) MAPSORT of: pFN21AE5468.seq Check: 5006 from: 1 to: 5653 pFN21AE5468 5653 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2996 1937 Size: 1059 4594 Acc65I G'GTAC_C Cuts at: 2568 2974 2568 Size: 406 5247 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2500 2897 2942 3716 5100 5368 1632 Size: 868 397 45 774 1384 268 1917 Fragments arranged by size: 1917 1384 868 774 397 268 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 4552 5529 1203 Size: 3349 977 1327 Fragments arranged by size: 3349 1327 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5653 AlwNI CAG_nnn'CTG Cuts at: 2647 3396 4968 2647 Size: 749 1572 3332 Fragments arranged by size: 3332 1572 749 ApaLI G'TGCA_C Cuts at: 2412 3710 4866 2412 Size: 1298 1156 3199 Fragments arranged by size: 3199 1298 1156 ApoI r'AATT_y Cuts at: 1493 2064 2213 2962 3188 3340 5449 1493 Size: 571 149 749 226 152 2109 1697 Fragments arranged by size: 2109 1697 749 571 226 152 149 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2978 1870 Size: 73 1035 4545 Fragments arranged by size: 4545 1035 73 BamHI G'GATC_C Cuts at: 2930 2983 3350 2930 Size: 53 367 5233 Fragments arranged by size: 5233 367 53 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2568 2974 4429 618 Size: 325 217 627 781 406 1455 1842 Fragments arranged by size: 1842 1455 781 627 406 325 217 BanII G_rGCy'C Cuts at: 1391 2972 1391 Size: 1581 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4682 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3844 3878 1903 Size: 34 1907 34 3678 Fragments arranged by size: 3678 1907 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 2448 1548 Size: 900 4753 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4264 136 Size: 107 122 71 631 605 2592 1525 Fragments arranged by size: 2592 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 3070 3070 Size: 5653 Bme1580I G_kGCm'C Cuts at: 1790 2416 3714 4870 1790 Size: 626 1298 1156 2573 Fragments arranged by size: 2573 1298 1156 626 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5653 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2069 3384 4342 Size: 416 53 83 399 24 652 1315 958 Cuts at: 4342 442 Size: 1753 Fragments arranged by size: 1753 1315 958 652 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2650 4313 1239 Size: 1411 1663 2579 Fragments arranged by size: 2579 1663 1411 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2185 2354 3778 4643 4941 2185 Size: 169 1424 865 298 2897 Fragments arranged by size: 2897 1424 865 298 169 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4316 882 Size: 617 2817 2219 Fragments arranged by size: 2817 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3444 493 Size: 711 2240 2702 Fragments arranged by size: 2702 2240 711 BsaBI GATnn'nnATC Cuts at: 5647 5647 Size: 5653 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3460 3842 4477 275 Size: 53 83 186 936 1927 382 635 1451 Fragments arranged by size: 1927 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2901 4086 4484 4758 4905 5316 2901 Size: 1185 398 274 147 411 3238 Fragments arranged by size: 3238 1185 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5428 Fragments arranged by size: 5428 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2542 1186 Size: 1356 4297 BseYI C'CCAG_C Cuts at: 4856 5352 4856 Size: 496 5157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5080 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 3864 4892 1540 Size: 275 186 1102 761 1028 2301 Fragments arranged by size: 2301 1102 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2416 3714 3799 4870 2416 Size: 1298 85 1071 3199 Fragments arranged by size: 3199 1298 1071 85 BsmI GAATG_Cn' Cuts at: 3164 3257 3164 Size: 93 5560 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2416 2972 3714 3799 4870 1391 Size: 399 626 556 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 626 556 399 85 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2580 3008 844 Size: 1736 428 3489 Fragments arranged by size: 3489 1736 428 BsrBI CCG'CTC Cuts at: 1394 1823 3540 1394 Size: 429 1717 3507 Fragments arranged by size: 3507 1717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4148 4322 65 Size: 1183 2900 174 1396 Fragments arranged by size: 2900 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4297 4484 1700 Size: 110 2487 187 2869 Fragments arranged by size: 2869 2487 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5653 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 3713 4725 3713 Size: 1012 4641 BstAPI GCAn_nnn'nTGC Cuts at: 794 2647 794 Size: 1853 3800 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5653 BstXI CCAn_nnnn'nTGG Cuts at: 2555 2555 Size: 5653 BstYI r'GATC_y Cuts at: 1972 2671 2930 2983 3350 3735 3752 5204 5290 Size: 699 259 53 367 385 17 1452 86 Cuts at: 5290 5302 5648 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 699 385 367 346 259 86 53 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5101 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4914 Fragments arranged by size: 4914 683 56 BtsI GCAGTG_nn' Cuts at: 2414 3169 3963 3983 2414 Size: 755 794 20 4084 Fragments arranged by size: 4084 794 755 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2293 2328 529 Size: 35 1729 35 3854 Fragments arranged by size: 3854 1729 35 35 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2839 3100 3989 Size: 54 1425 50 275 18 1009 261 889 Cuts at: 3989 5618 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1009 889 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2519 2735 3583 1353 Size: 1166 216 848 3423 Fragments arranged by size: 3423 1166 848 216 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4240 4614 4760 387 Size: 1064 138 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2500 2650 2897 2942 3716 4313 5100 Size: 393 868 150 247 45 774 597 787 Cuts at: 5100 5368 1239 Size: 268 1524 Fragments arranged by size: 1524 868 787 774 597 393 268 247 150 45 EcoO109I rG'GnC_Cy Cuts at: 2939 2939 Size: 5653 EcoRI G'AATT_C Cuts at: 2064 2213 2962 3340 2064 Size: 149 749 378 4377 Fragments arranged by size: 4377 749 378 149 FspI TGC'GCA Cuts at: 4159 4159 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 4800 1678 Size: 3122 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4294 4326 4368 4400 1373 Size: 32 135 32 2722 32 42 32 2626 Fragments arranged by size: 2722 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2997 3243 669 Size: 988 281 1059 246 3079 Fragments arranged by size: 3079 1059 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KpnI G_GTAC'C Cuts at: 2572 2978 2572 Size: 406 5247 MfeI C'AATT_G Cuts at: 2074 3252 2074 Size: 1178 4475 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2699 4767 4951 1305 Size: 1394 2068 184 2007 Fragments arranged by size: 2068 2007 1394 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2841 10 Size: 54 1425 343 1009 2822 Fragments arranged by size: 2822 1425 1009 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3611 3970 4129 518 Size: 648 2445 359 159 2042 Fragments arranged by size: 2445 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2106 3069 3746 4894 5139 1606 Size: 500 963 677 1148 245 2120 Fragments arranged by size: 2120 1148 963 677 500 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5101 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 3011 4556 1132 Size: 97 1782 1545 2229 Fragments arranged by size: 2229 1782 1545 97 PasI CC'CwG_GG Cuts at: 1348 2623 1348 Size: 1275 4378 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5653 PfoI T'CCnGG_A Cuts at: 2267 2267 Size: 5653 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2012 2044 3722 3754 5235 5267 2012 Size: 32 1678 32 1481 32 2398 Fragments arranged by size: 2398 1678 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2939 2939 Size: 5653 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PstI C_TGCA'G Cuts at: 830 3005 830 Size: 2175 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 PvuII CAG'CTG Cuts at: 2106 2106 Size: 5653 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SanDI GG'GwC_CC Cuts at: 2939 2939 Size: 5653 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 3901 5613 3901 Size: 1712 3941 SfcI C'TryA_G Cuts at: 826 1045 3001 4136 4817 5008 826 Size: 219 1956 1135 681 191 1471 Fragments arranged by size: 1956 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2164 2369 3757 4658 4920 820 Size: 197 926 221 205 1388 901 262 1553 Fragments arranged by size: 1553 1388 926 901 262 221 205 197 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 2934 3092 513 Size: 552 1869 158 3074 Fragments arranged by size: 3074 1869 552 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2399 3680 3697 3850 4035 4480 325 Size: 1255 819 1281 17 153 185 445 1498 Fragments arranged by size: 1498 1281 1255 819 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3899 5611 96 Size: 274 80 33 51 1443 1922 1712 138 Fragments arranged by size: 1922 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5613 5645 5613 Size: 32 5621 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5653 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI