(Circular) (Six-base) MAPSORT of: pFN21AE2335.seq Check: 9303 from: 1 to: 9145 pFN21AE2335 9145 bp With 182 enzymes: SgfI * May 11, 2017 14:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4901 4901 Size: 9145 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6955 278 Size: 53 83 186 936 5419 2468 Fragments arranged by size: 5419 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3690 4807 6488 1937 Size: 1753 1117 1681 4594 Fragments arranged by size: 4594 1753 1681 1117 Acc65I G'GTAC_C Cuts at: 6466 6466 Size: 9145 AclI AA'CG_TT Cuts at: 1563 7272 7645 1563 Size: 5709 373 3063 Fragments arranged by size: 5709 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3277 3420 4271 4783 5160 5814 7208 8592 Size: 1645 143 851 512 377 654 1394 1384 Cuts at: 8592 8860 1632 Size: 268 1917 Fragments arranged by size: 1917 1645 1394 1384 851 654 512 377 268 143 AflII C'TTAA_G Cuts at: 820 1017 2866 820 Size: 197 1849 7099 Fragments arranged by size: 7099 1849 197 AflIII A'CryG_T Cuts at: 1203 4899 5593 8044 9021 1203 Size: 3696 694 2451 977 1327 Fragments arranged by size: 3696 2451 1327 977 694 AgeI A'CCGG_T Cuts at: 7976 7976 Size: 9145 AhdI GACnn_n'nnGTC Cuts at: 7874 7874 Size: 9145 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9145 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3056 1184 Size: 1872 7273 AlwNI CAG_nnn'CTG Cuts at: 2098 4229 6292 6888 8460 2098 Size: 2131 2063 596 1572 2783 Fragments arranged by size: 2783 2131 2063 1572 596 ApaLI G'TGCA_C Cuts at: 7202 8358 7202 Size: 1156 7989 ApoI r'AATT_y Cuts at: 1493 2573 3465 3669 3979 4099 4133 4842 5251 Size: 1080 892 204 310 120 34 709 409 Cuts at: 5251 5587 6454 6680 6832 8941 1493 Size: 336 867 226 152 2109 1697 Fragments arranged by size: 2109 1697 1080 892 867 709 409 336 310 226 204 152 120 34 AseI AT'TA_AT Cuts at: 160 1032 2214 3336 3854 5000 7699 160 Size: 872 1182 1122 518 1146 2699 1606 Fragments arranged by size: 2699 1606 1182 1146 1122 872 518 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9145 AvaI C'yCGr_G Cuts at: 1870 1943 6470 1870 Size: 73 4527 4545 Fragments arranged by size: 4545 4527 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3306 3339 3306 Size: 33 9112 BamHI G'GATC_C Cuts at: 3377 5917 6133 6475 6842 3377 Size: 2540 216 342 367 5680 Fragments arranged by size: 5680 2540 367 342 216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3503 6466 7921 618 Size: 325 217 627 1716 2963 1455 1842 Fragments arranged by size: 2963 1842 1716 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 6464 1391 Size: 5073 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2498 4491 928 Size: 971 599 1993 5582 Fragments arranged by size: 5582 1993 971 599 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6085 6119 7336 7370 1903 Size: 34 50 34 4064 34 1217 34 3678 Fragments arranged by size: 4064 3678 1217 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2078 3725 7037 8247 2078 Size: 1647 3312 1210 2976 Fragments arranged by size: 3312 2976 1647 1210 BclI T'GATC_A Cuts at: 1548 4266 5938 1548 Size: 2718 1672 4755 Fragments arranged by size: 4755 2718 1672 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4400 7756 136 Size: 107 122 71 631 605 2728 3356 1525 Fragments arranged by size: 3356 2728 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5644 5978 6077 6099 9140 5644 Size: 334 99 22 3041 5649 Fragments arranged by size: 5649 3041 334 99 22 BlpI GC'TnA_GC Cuts at: 2926 6562 2926 Size: 3636 5509 Bme1580I G_kGCm'C Cuts at: 1790 7206 8362 1790 Size: 5416 1156 2573 Fragments arranged by size: 5416 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9145 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2318 6114 6876 Size: 416 53 83 399 24 901 3796 762 Cuts at: 6876 7834 442 Size: 958 1753 Fragments arranged by size: 3796 1753 958 901 762 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9145 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2034 2371 3610 2034 Size: 337 1239 7569 Fragments arranged by size: 7569 1239 337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2260 3405 3449 4095 5434 7805 1239 Size: 1021 1145 44 646 1339 2371 2579 Fragments arranged by size: 2579 2371 1339 1145 1021 646 44 Bpu10I CC'TnA_GC Cuts at: 6041 6041 Size: 9145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4428 6352 7270 8135 8433 4428 Size: 1924 918 865 298 5140 Fragments arranged by size: 5140 1924 918 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3989 5978 7808 882 Size: 617 2490 1989 1830 2219 Fragments arranged by size: 2490 2219 1989 1830 617 BsaAI yAC'GTr Cuts at: 493 1204 4762 5596 6936 493 Size: 711 3558 834 1340 2702 Fragments arranged by size: 3558 2702 1340 834 711 BsaBI GATnn'nnATC Cuts at: 9139 9139 Size: 9145 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2666 6952 7334 7969 Size: 53 83 186 936 1133 4286 382 635 Cuts at: 7969 275 Size: 1451 Fragments arranged by size: 4286 1451 1133 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3003 6360 7578 7976 8250 8397 8808 3003 Size: 3357 1218 398 274 147 411 3340 Fragments arranged by size: 3357 3340 1218 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8920 Fragments arranged by size: 8920 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2624 3224 4656 6319 6425 1186 Size: 1438 600 1432 1663 106 3906 Fragments arranged by size: 3906 1663 1438 1432 600 106 BseYI C'CCAG_C Cuts at: 2422 2717 2759 2832 8348 8844 2422 Size: 295 42 73 5516 496 2723 Fragments arranged by size: 5516 2723 496 295 73 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3987 1163 Size: 573 2251 6321 Fragments arranged by size: 6321 2251 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6595 7356 8384 1540 Size: 275 186 4594 761 1028 2301 Fragments arranged by size: 4594 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 6127 7206 7291 8362 6127 Size: 1079 85 1071 6910 Fragments arranged by size: 6910 1079 1071 85 BsmI GAATG_Cn' Cuts at: 2457 6236 6656 6749 2457 Size: 3779 420 93 4853 Fragments arranged by size: 4853 3779 420 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 6127 6464 7206 7291 8362 1391 Size: 399 4337 337 742 85 1071 2174 Fragments arranged by size: 4337 2174 1071 742 399 337 85 BspEI T'CCGG_A Cuts at: 3003 8808 3003 Size: 5805 3340 BspHI T'CATG_A Cuts at: 5421 7034 8764 5421 Size: 1613 1730 5802 Fragments arranged by size: 5802 1730 1613 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4901 6500 844 Size: 4057 1599 3489 Fragments arranged by size: 4057 3489 1599 BsrBI CCG'CTC Cuts at: 1394 1823 7032 1394 Size: 429 5209 3507 Fragments arranged by size: 5209 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2850 5337 5429 6228 7640 7814 65 Size: 1183 1602 2487 92 799 1412 174 1396 Fragments arranged by size: 2487 1602 1412 1396 1183 799 174 92 BsrFI r'CCGG_y Cuts at: 1700 1810 7789 7976 1700 Size: 110 5979 187 2869 Fragments arranged by size: 5979 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3076 96 Size: 2980 6165 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9145 BssSI C'ACGA_G Cuts at: 7205 8217 7205 Size: 1012 8133 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9145 BstBI TT'CG_AA Cuts at: 7949 7949 Size: 9145 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9145 BstXI CCAn_nnnn'nTGG Cuts at: 5813 5813 Size: 9145 BstYI r'GATC_y Cuts at: 1972 3377 5644 5917 5978 6077 6099 6133 6475 Size: 1405 2267 273 61 99 22 34 342 Cuts at: 6475 6842 7227 7244 8696 8782 8794 9140 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2267 1977 1452 1405 385 367 346 342 273 99 86 61 34 22 17 12 BstZ17I GTA'TAC Cuts at: 3691 3691 Size: 9145 Bsu36I CC'TnA_GG Cuts at: 4398 5620 4398 Size: 1222 7923 BtgI C'CryG_G Cuts at: 513 1065 6046 513 Size: 552 4981 3612 Fragments arranged by size: 4981 3612 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2242 487 Size: 683 56 1016 7390 Fragments arranged by size: 7390 1016 683 56 BtsI GCAGTG_nn' Cuts at: 2408 4536 4722 5288 5441 6661 7455 7475 2408 Size: 2128 186 566 153 1220 794 20 4078 Fragments arranged by size: 4078 2128 1220 794 566 186 153 20 ClaI AT'CG_AT Cuts at: 1554 5811 1554 Size: 4257 4888 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9110 DraI TTT'AAA Cuts at: 2499 3834 4066 6449 2499 Size: 1335 232 2383 5195 Fragments arranged by size: 5195 2383 1335 232 DraIII CAC_nnn'GTG Cuts at: 3650 5201 3650 Size: 1551 7594 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8152 809 Size: 507 6836 1802 Fragments arranged by size: 6836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5557 5713 6049 Size: 54 1425 50 275 18 3727 156 336 Cuts at: 6049 6592 7481 9110 8 Size: 543 889 1629 43 Fragments arranged by size: 3727 1629 1425 889 543 336 275 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6592 1537 Size: 275 4780 4090 Fragments arranged by size: 4780 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3293 3579 4173 4674 5380 5747 6249 6317 Size: 1940 286 594 501 706 367 502 68 Cuts at: 6317 6417 7075 1353 Size: 100 658 3423 Fragments arranged by size: 3423 1940 706 658 594 502 501 367 286 100 68 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7732 8106 8252 387 Size: 1064 138 6143 374 146 1280 Fragments arranged by size: 6143 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2260 3277 3405 3420 3449 4095 4271 Size: 393 628 1017 128 15 29 646 176 Cuts at: 4271 4783 5160 5434 5814 7208 7805 8592 8860 Size: 512 377 274 380 1394 597 787 268 Cuts at: 8860 1239 Size: 1524 Fragments arranged by size: 1524 1394 1017 787 646 628 597 512 393 380 377 274 268 176 128 29 15 EcoNI CCTnn'n_nnAGG Cuts at: 2711 2711 Size: 9145 EcoRI G'AATT_C Cuts at: 4099 5587 6454 6832 4099 Size: 1488 867 378 6412 Fragments arranged by size: 6412 1488 867 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3690 4174 4516 6028 3690 Size: 484 342 1512 6807 Fragments arranged by size: 6807 1512 484 342 FspI TGC'GCA Cuts at: 3044 7651 3044 Size: 4607 4538 HaeII r_GCGC'y Cuts at: 1678 2098 8292 1678 Size: 420 6194 2531 Fragments arranged by size: 6194 2531 420 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 3640 3672 3911 Size: 32 135 32 563 32 1473 32 239 Cuts at: 3911 3943 4237 4269 5804 5836 6069 6101 6385 Size: 32 294 32 1535 32 233 32 284 Cuts at: 6385 6417 7786 7818 7860 7892 1373 Size: 32 1369 32 42 32 2626 Fragments arranged by size: 2626 1535 1473 1369 563 294 284 239 233 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3659 4336 4890 5286 5538 6489 Size: 988 281 1721 677 554 396 252 951 Cuts at: 6489 6735 669 Size: 246 3079 Fragments arranged by size: 3079 1721 988 951 677 554 396 281 252 246 HindIII A'AGCT_T Cuts at: 748 5176 6005 748 Size: 4428 829 3888 Fragments arranged by size: 4428 3888 829 HpaI GTT'AAC Cuts at: 4336 6735 4336 Size: 2399 6746 KpnI G_GTAC'C Cuts at: 6470 6470 Size: 9145 MfeI C'AATT_G Cuts at: 6744 6744 Size: 9145 MluI A'CGCG_T Cuts at: 9021 9021 Size: 9145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2583 6360 8259 8443 1305 Size: 1278 3777 1899 184 2007 Fragments arranged by size: 3777 2007 1899 1278 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5559 5715 6051 10 Size: 54 1425 343 3727 156 336 3104 Fragments arranged by size: 3727 3104 1425 343 336 156 54 MslI CAynn'nnrTG Cuts at: 518 1166 2450 4262 4760 5434 5459 5507 7103 Size: 648 1284 1812 498 674 25 48 1596 Cuts at: 7103 7462 7621 518 Size: 359 159 2042 Fragments arranged by size: 2042 1812 1596 1284 674 648 498 359 159 48 25 MspA1I CmG'CkG Cuts at: 1606 2058 6561 7238 8386 8631 1606 Size: 452 4503 677 1148 245 2120 Fragments arranged by size: 4503 2120 1148 677 452 245 NcoI C'CATG_G Cuts at: 513 1065 6046 513 Size: 552 4981 3612 Fragments arranged by size: 4981 3612 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9145 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9145 NotI GC'GGCC_GC Cuts at: 6592 6592 Size: 9145 NruI TCG'CGA Cuts at: 2363 2363 Size: 9145 NspI r_CATG'y Cuts at: 1132 1229 3576 5512 6503 8048 1132 Size: 97 2347 1936 991 1545 2229 Fragments arranged by size: 2347 2229 1936 1545 991 97 PasI CC'CwG_GG Cuts at: 1348 3069 1348 Size: 1721 7424 PciI A'CATG_T Cuts at: 8044 8044 Size: 9145 PflMI CCAn_nnn'nTGG Cuts at: 1108 5722 1108 Size: 4614 4531 PfoI T'CCnGG_A Cuts at: 3488 4037 3488 Size: 549 8596 PmeI GTTT'AAAC Cuts at: 6449 6449 Size: 9145 PmlI CAC'GTG Cuts at: 5596 5596 Size: 9145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7214 7246 8727 8759 7214 Size: 32 1481 32 7600 Fragments arranged by size: 7600 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9145 PsiI TTA'TAA Cuts at: 2487 6715 2487 Size: 4228 4917 PstI C_TGCA'G Cuts at: 830 6497 830 Size: 5667 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9145 PvuII CAG'CTG Cuts at: 2058 2058 Size: 9145 SalI G'TCGA_C Cuts at: 1936 6487 1936 Size: 4551 4594 SbfI CC_TGCA'GG Cuts at: 6497 6497 Size: 9145 ScaI AGT'ACT Cuts at: 4690 5758 7393 9105 4690 Size: 1068 1635 1712 4730 Fragments arranged by size: 4730 1712 1635 1068 SfcI C'TryA_G Cuts at: 826 1045 3171 3911 5016 6493 7628 8309 8500 Size: 219 2126 740 1105 1477 1135 681 191 Cuts at: 8500 826 Size: 1471 Fragments arranged by size: 2126 1477 1471 1135 1105 740 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9145 SmaI CCC'GGG Cuts at: 1872 6472 1872 Size: 4600 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2866 4443 6367 7249 8150 8412 Size: 197 926 923 1577 1924 882 901 262 Cuts at: 8412 820 Size: 1553 Fragments arranged by size: 1924 1577 1553 926 923 901 882 262 197 SnaBI TAC'GTA Cuts at: 493 6936 493 Size: 6443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9145 SphI G_CATG'C Cuts at: 1132 3576 6503 1132 Size: 2444 2927 3774 Fragments arranged by size: 3774 2927 2444 SspI AAT'ATT Cuts at: 5 52 3012 3762 3852 7069 5 Size: 47 2960 750 90 3217 2081 Fragments arranged by size: 3217 2960 2081 750 90 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9145 StyI C'CwwG_G Cuts at: 513 1065 4339 4654 6046 6584 513 Size: 552 3274 315 1392 538 3074 Fragments arranged by size: 3274 3074 1392 552 538 315 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2436 7172 7189 7342 7527 7972 325 Size: 1255 856 4736 17 153 185 445 1498 Fragments arranged by size: 4736 1498 1255 856 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2293 2742 3076 Size: 274 80 33 51 1443 316 449 334 Cuts at: 3076 4688 5426 5756 7391 9103 96 Size: 1612 738 330 1635 1712 138 Fragments arranged by size: 1712 1635 1612 1443 738 449 334 330 316 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2755 2787 5185 5217 9105 9137 2755 Size: 32 2398 32 3888 32 2763 Fragments arranged by size: 3888 2763 2398 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6014 1316 Size: 4698 4447 XbaI T'CTAG_A Cuts at: 2518 6481 2518 Size: 3963 5182 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5458 6127 5458 Size: 669 8476 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9145 XmaI C'CCGG_G Cuts at: 1870 6470 1870 Size: 4600 4545 XmnI GAAnn'nnTTC Cuts at: 3011 3265 3466 3979 4135 5994 7274 3011 Size: 254 201 513 156 1859 1280 4882 Fragments arranged by size: 4882 1859 1280 513 254 201 156 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6953 276 Size: 53 83 186 936 5419 2468 Fragments arranged by size: 5419 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI