Restriction map for pFN21AE2335
 (Linear) (Six-base) MAPSORT of: KIEE2335.seq  Check: 5621  from: 1  to: 4440



KIEE2335.orf

 With 182 enzymes: SgfI * 

                        May 11, 2017 11:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2896    4440 
   Size:       2896    1544
 
AccI GT'mk_AC

Cuts at:      0    1685    2802    4440 
   Size:       1685    1117    1638

  Fragments arranged by size: 

                1685    1638    1117
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1272    1415    2266    2778    3155    3809    4440 
   Size:       1272     143     851     512     377     654     631

  Fragments arranged by size: 

                1272     851     654     631     512     377     143
 
AflII C'TTAA_G

Cuts at:      0     861    4440 
   Size:        861    3579
 
AflIII A'CryG_T

Cuts at:      0    2894    3588    4440 
   Size:       2894     694     852

  Fragments arranged by size: 

                2894     852     694
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1051    4440 
   Size:       1051    3389
 
AlwNI CAG_nnn'CTG

Cuts at:      0      93    2224    4287    4440 
   Size:         93    2131    2063     153

  Fragments arranged by size: 

                2131    2063     153      93
 
ApoI r'AATT_y

Cuts at:      0     568    1460    1664    1974    2094    2128    2837    3246 
   Size:        568     892     204     310     120      34     709     409

Cuts at:   3246    3582    4440 
   Size:        336     858

  Fragments arranged by size: 

                 892     858     709     568     409     336     310     204
                 120      34
 
AseI AT'TA_AT

Cuts at:      0     209    1331    1849    2995    4440 
   Size:        209    1122     518    1146    1445

  Fragments arranged by size: 

                1445    1146    1122     518     209
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1301    1334    4440 
   Size:       1301      33    3106

  Fragments arranged by size: 

                3106    1301      33
 
BamHI G'GATC_C

Cuts at:      0    1372    3912    4128    4440 
   Size:       1372    2540     216     312

  Fragments arranged by size: 

                2540    1372     312     216
 
BanI G'GyrC_C

Cuts at:      0    1498    4440 
   Size:       1498    2942
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     493    2486    4440 
   Size:        493    1993    1954

  Fragments arranged by size: 

                1993    1954     493
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4080    4114    4440 
   Size:       4080      34     326

  Fragments arranged by size: 

                4080     326      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      73    1720    4440 
   Size:         73    1647    2720

  Fragments arranged by size: 

                2720    1647      73
 
BclI T'GATC_A

Cuts at:      0    2261    3933    4440 
   Size:       2261    1672     507

  Fragments arranged by size: 

                2261    1672     507
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2395    4440 
   Size:       2395    2045
 
BglII A'GATC_T

Cuts at:      0    3639    3973    4072    4094    4440 
   Size:       3639     334      99      22     346

  Fragments arranged by size: 

                3639     346     334      99      22
 
BlpI GC'TnA_GC

Cuts at:      0     921    4440 
   Size:        921    3519
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     313    4109    4440 
   Size:        313    3796     331

  Fragments arranged by size: 

                3796     331     313
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      29     366    1605    4440 
   Size:         29     337    1239    2835

  Fragments arranged by size: 

                2835    1239     337      29
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     255    1400    1444    2090    3429    4440 
   Size:        255    1145      44     646    1339    1011

  Fragments arranged by size: 

                1339    1145    1011     646     255      44
 
Bpu10I CC'TnA_GC

Cuts at:      0    4036    4440 
   Size:       4036     404
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2423    4347    4440 
   Size:       2423    1924      93

  Fragments arranged by size: 

                2423    1924      93
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1984    3973    4440 
   Size:       1984    1989     467

  Fragments arranged by size: 

                1989    1984     467
 
BsaAI yAC'GTr

Cuts at:      0    2757    3591    4440 
   Size:       2757     834     849

  Fragments arranged by size: 

                2757     849     834
 
BsaHI Gr'CG_yC

Cuts at:      0     661    4440 
   Size:        661    3779
 
BsaWI w'CCGG_w

Cuts at:      0     998    4355    4440 
   Size:        998    3357      85

  Fragments arranged by size: 

                3357     998      85
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     619    1219    2651    4314    4420    4440 
   Size:        619     600    1432    1663     106      20

  Fragments arranged by size: 

                1663    1432     619     600     106      20
 
BseYI C'CCAG_C

Cuts at:      0     417     712     754     827    4440 
   Size:        417     295      42      73    3613

  Fragments arranged by size: 

                3613     417     295      73      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1982    4440 
   Size:       1982    2458
 
BsiHKAI G_wGCw'C

Cuts at:      0    4122    4440 
   Size:       4122     318
 
BsmI GAATG_Cn'

Cuts at:      0     452    4231    4440 
   Size:        452    3779     209

  Fragments arranged by size: 

                3779     452     209
 
Bsp1286I G_dGCh'C

Cuts at:      0    4122    4440 
   Size:       4122     318
 
BspEI T'CCGG_A

Cuts at:      0     998    4440 
   Size:        998    3442
 
BspHI T'CATG_A

Cuts at:      0    3416    4440 
   Size:       3416    1024
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2896    4440 
   Size:       2896    1544
 
BsrDI GCAATG_nn'

Cuts at:      0     845    3332    3424    4223    4440 
   Size:        845    2487      92     799     217

  Fragments arranged by size: 

                2487     845     799     217      92
 
BsrGI T'GTAC_A

Cuts at:      0    1071    4440 
   Size:       1071    3369
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3808    4440 
   Size:       3808     632
 
BstYI r'GATC_y

Cuts at:      0    1372    3639    3912    3973    4072    4094    4128    4440 
   Size:       1372    2267     273      61      99      22      34     312

  Fragments arranged by size: 

                2267    1372     312     273      99      61      34      22
            
 
BstZ17I GTA'TAC

Cuts at:      0    1686    4440 
   Size:       1686    2754
 
Bsu36I CC'TnA_GG

Cuts at:      0    2393    3615    4440 
   Size:       2393    1222     825

  Fragments arranged by size: 

                2393    1222     825
 
BtgI C'CryG_G

Cuts at:      0    4041    4440 
   Size:       4041     399
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     237    4440 
   Size:        237    4203
 
BtsI GCAGTG_nn'

Cuts at:      0     403    2531    2717    3283    3436    4440 
   Size:        403    2128     186     566     153    1004

  Fragments arranged by size: 

                2128    1004     566     403     186     153
 
ClaI AT'CG_AT

Cuts at:      0    3806    4440 
   Size:       3806     634
 
DraI TTT'AAA

Cuts at:      0     494    1829    2061    4440 
   Size:        494    1335     232    2379

  Fragments arranged by size: 

                2379    1335     494     232
 
DraIII CAC_nnn'GTG

Cuts at:      0    1645    3196    4440 
   Size:       1645    1551    1244

  Fragments arranged by size: 

                1645    1551    1244
 
EaeI y'GGCC_r

Cuts at:      0    3552    3708    4044    4440 
   Size:       3552     156     336     396

  Fragments arranged by size: 

                3552     396     336     156
 
EarI CTCTTCn'nnn_

Cuts at:      0    1288    1574    2168    2669    3375    3742    4244    4312 
   Size:       1288     286     594     501     706     367     502      68

Cuts at:   4312    4412    4440 
   Size:        100      28

  Fragments arranged by size: 

                1288     706     594     502     501     367     286     100
                  68      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     255    1272    1400    1415    1444    2090    2266    2778 
   Size:        255    1017     128      15      29     646     176     512

Cuts at:   2778    3155    3429    3809    4440 
   Size:        377     274     380     631

  Fragments arranged by size: 

                1017     646     631     512     380     377     274     255
                 176     128      29      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     706    4440 
   Size:        706    3734
 
EcoRI G'AATT_C

Cuts at:      0    2094    3582    4440 
   Size:       2094    1488     858

  Fragments arranged by size: 

                2094    1488     858
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1685    2169    2511    4023    4440 
   Size:       1685     484     342    1512     417

  Fragments arranged by size: 

                1685    1512     484     417     342
 
FspI TGC'GCA

Cuts at:      0    1039    4440 
   Size:       1039    3401
 
HaeII r_GCGC'y

Cuts at:      0      93    4440 
   Size:         93    4347
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162    1635    1667    1906    1938    2232    2264 
   Size:        130      32    1473      32     239      32     294      32

Cuts at:   2264    3799    3831    4064    4096    4380    4412    4440 
   Size:       1535      32     233      32     284      32      28

  Fragments arranged by size: 

                1535    1473     294     284     239     233     130      32
                  32      32      32      32      32      32      28
 
HincII GTy'rAC

Cuts at:      0    1654    2331    2885    3281    3533    4440 
   Size:       1654     677     554     396     252     907

  Fragments arranged by size: 

                1654     907     677     554     396     252
 
HindIII A'AGCT_T

Cuts at:      0    3171    4000    4440 
   Size:       3171     829     440

  Fragments arranged by size: 

                3171     829     440
 
HpaI GTT'AAC

Cuts at:      0    2331    4440 
   Size:       2331    2109
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     578    4355    4440 
   Size:        578    3777      85

  Fragments arranged by size: 

                3777     578      85
 
MscI TGG'CCA

Cuts at:      0    3554    3710    4046    4440 
   Size:       3554     156     336     394

  Fragments arranged by size: 

                3554     394     336     156
 
MslI CAynn'nnrTG

Cuts at:      0     445    2257    2755    3429    3454    3502    4440 
   Size:        445    1812     498     674      25      48     938

  Fragments arranged by size: 

                1812     938     674     498     445      48      25
 
MspA1I CmG'CkG

Cuts at:      0      53    4440 
   Size:         53    4387
 
NcoI C'CATG_G

Cuts at:      0    4041    4440 
   Size:       4041     399
 
NruI TCG'CGA

Cuts at:      0     358    4440 
   Size:        358    4082
 
NspI r_CATG'y

Cuts at:      0    1571    3507    4440 
   Size:       1571    1936     933

  Fragments arranged by size: 

                1936    1571     933
 
PasI CC'CwG_GG

Cuts at:      0    1064    4440 
   Size:       1064    3376
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3717    4440 
   Size:       3717     723
 
PfoI T'CCnGG_A

Cuts at:      0    1483    2032    4440 
   Size:       1483     549    2408

  Fragments arranged by size: 

                2408    1483     549
 
PmlI CAC'GTG

Cuts at:      0    3591    4440 
   Size:       3591     849
 
PsiI TTA'TAA

Cuts at:      0     482    4440 
   Size:        482    3958
 
PvuII CAG'CTG

Cuts at:      0      53    4440 
   Size:         53    4387
 
ScaI AGT'ACT

Cuts at:      0    2685    3753    4440 
   Size:       2685    1068     687

  Fragments arranged by size: 

                2685    1068     687
 
SfcI C'TryA_G

Cuts at:      0    1166    1906    3011    4440 
   Size:       1166     740    1105    1429

  Fragments arranged by size: 

                1429    1166    1105     740
 
SmlI C'TyrA_G

Cuts at:      0     861    2438    4362    4440 
   Size:        861    1577    1924      78

  Fragments arranged by size: 

                1924    1577     861      78
 
SphI G_CATG'C

Cuts at:      0    1571    4440 
   Size:       1571    2869
 
SspI AAT'ATT

Cuts at:      0    1007    1757    1847    4440 
   Size:       1007     750      90    2593

  Fragments arranged by size: 

                2593    1007     750      90
 
StyI C'CwwG_G

Cuts at:      0    2334    2649    4041    4440 
   Size:       2334     315    1392     399

  Fragments arranged by size: 

                2334    1392     399     315
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     431    4440 
   Size:        431    4009
 
TatI w'GTAC_w

Cuts at:      0     288     737    1071    2683    3421    3751    4440 
   Size:        288     449     334    1612     738     330     689

  Fragments arranged by size: 

                1612     738     689     449     334     330     288
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     750     782    3180    3212    4440 
   Size:        750      32    2398      32    1228

  Fragments arranged by size: 

                2398    1228     750      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    4009    4440 
   Size:       4009     431
 
XbaI T'CTAG_A

Cuts at:      0     513    4440 
   Size:        513    3927
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3453    4122    4440 
   Size:       3453     669     318

  Fragments arranged by size: 

                3453     669     318
 
XmnI GAAnn'nnTTC

Cuts at:      0    1006    1260    1461    1974    2130    3989    4440 
   Size:       1006     254     201     513     156    1859     451

  Fragments arranged by size: 

                1859    1006     513     451     254     201     156
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AlfI    AlwNI     ApoI
     AseI     BaeI    BamHI     BanI     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrGI    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI
   DraIII     EaeI     EarI  Eco57MI    EcoNI    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     MmeI     MscI     MslI
   MspA1I     NcoI     NruI     NspI     PasI    PflMI     PfoI     PmlI
     PsiI    PvuII     ScaI     SfcI     SmlI     SphI     SspI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI     AloI
     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII    BanII     BbeI
    BbvCI    BfrBI Bme1580I    BmgBI     BmtI    BsaBI    BsaXI    BsiEI
    BsiWI    BsmBI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    CspCI     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRV
     FseI    FspAI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NsiI     PacI     PciI     PmeI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     StuI     SwaI     XhoI     XmaI     ZraI
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