################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI HphI KasI KpnI Ksp632I MaeI MaeII MamI MfeI MroI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflIII 191 ACGCGT A^C[GA][CT]GT AlwNI 157 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 63 CCTTAGG CC^T[ACGT]AGG ApaI 176 GGGCCC GGGCC^C AspHI 195 GTGCTC G[AT]GC[AT]^C AvaI 179 CCCGGG C^[CT]CG[GA]G AvaII 281 GGTCC G^G[AT]CC BalI 227 TGGCCA TGG^CCA BglI 139 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 203 AGATCT A^GATCT BpmI 331 CTGGAG CTGGAG(16/14) BsiHKAI 195 GTGCTC G[AT]GC[AT]^C BsiYI 344 CCGACACACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 344 CCGACACACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 179 CCCGGG C^[CT]CG[GA]G BspMI 288 GCAGGT ACCTGC(4/8) BstNI 225 CCTGG CC^[AT]GG Bsu36I 63 CCTTAGG CC^T[ACGT]AGG DrdI 106 GACATTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 227 TGGCCA [CT]^GGCC[GA] Eco81I 63 CCTTAGG CC^T[ACGT]AGG EcoRII 225 CCTGG ^CC[AT]GG HincII 115 GTCAAC GT[CT]^[GA]AC HindII 115 GTCAAC GT[CT]^[GA]AC KspI 21 CCGCGG CCGC^GG MaeIII 409 GTCAC ^GT[ACGT]AC MluI 191 ACGCGT A^CGCGT MluNI 227 TGGCCA TGG^CCA MscI 227 TGGCCA TGG^CCA MseI 134 TTAA T^TAA MvaI 225 CCTGG CC^[AT]GG NlaIII 120 CATG CATG^ PpuMI 280 GGGTCCT [GA]G^G[AT]CC[CT] SacII 21 CCGCGG CCGC^GG SfiI 138 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 179 CCCGGG CCC^GGG Tru9I 134 TTAA T^TAA Tsp45I 409 GTCAC ^GT[GC]AC XmaCI 179 CCCGGG C^CCGGG XmaI 179 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### BamHI 45 GGATCC G^GATCC 368 GGATCC BanII 16 GAGCCC G[GA]GC[CT]^C 176 GGGCCC BbsI 123 GAAGAC GAAGAC(2/6) 400 GTCTTC BpuAI 123 GAAGAC GAAGAC(2/6) 400 GTCTTC BsaI 96 GAGACC GGTCTC(1/5) 340 GAGACC BsmAI 96 GAGAC GTCTC(1/5) 340 GAGAC BsrI 42 ACTGG ACTGG(1/-1) 49 CCAGT BstF5I 387 CATCC GGATG(2/0) 393 CATCC CfoI 239 GCGC GCG^C 253 GCGC DraII 175 GGGGCCC [GA]G^G[ACGT]CC[CT] 280 GGGTCCT DsaI 21 CCGCGG C^C[GA][CT]GG 311 CCGTGG EcoO109I 175 GGGGCCC [GA]G^G[ACGT]CC[CT] 280 GGGTCCT FokI 387 CATCC GGATG(9/13) 393 CATCC HapII 93 CCGG C^CGG 180 CCGG HhaI 239 GCGC GCG^C 253 GCGC HinP1I 239 GCGC G^CGC 253 GCGC HinfI 363 GACTC G^A[ACGT]TC 398 GAGTC HpaII 93 CCGG C^CGG 180 CCGG MboII 123 GAAGA GAAGA(8/7) 401 TCTTC MspI 93 CCGG C^CGG 180 CCGG PleI 363 GACTC GAGTC(4/5) 398 GAGTC PstI 256 CTGCAG CTGCA^G 262 CTGCAG PvuII 142 CAGCTG CAG^CTG 259 CAGCTG SfcI 256 CTGCAG C^T[GA][CT]AG 262 CTGCAG TspRI 50 CAGTG CA[GC]TG[ACGT][ACGT]^ 359 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AccII AciI AluI AlwI BbvI BcnI BmyI BsaJI Bsp1286I BssKI BstUI BstYI Cac8I Cfr13I DdeI DpnII FauI Fnu4HI HaeIII ItaI MboI MflI MnlI MspA1I MvnI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI TseI XhoII