(Circular) (Six-base) MAPSORT of: pFN21AE5165.seq Check: 3486 from: 1 to: 5125 pFN21AE5165 5125 bp With 182 enzymes: SgfI * January 30, 2014 17:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2284 2284 Size: 5125 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2935 278 Size: 53 83 186 936 1399 2468 Fragments arranged by size: 2468 1399 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2468 1937 Size: 531 4594 Acc65I G'GTAC_C Cuts at: 2446 2446 Size: 5125 AclI AA'CG_TT Cuts at: 1563 3252 3625 1563 Size: 1689 373 3063 Fragments arranged by size: 3063 1689 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2392 3188 4572 4840 1632 Size: 760 796 1384 268 1917 Fragments arranged by size: 1917 1384 796 760 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4928 AflIII A'CryG_T Cuts at: 1203 2196 4024 5001 1203 Size: 993 1828 977 1327 Fragments arranged by size: 1828 1327 993 977 AgeI A'CCGG_T Cuts at: 3956 3956 Size: 5125 AhdI GACnn_n'nnGTC Cuts at: 3854 3854 Size: 5125 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5125 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2316 1184 Size: 1132 3993 AlwNI CAG_nnn'CTG Cuts at: 2167 2868 4440 2167 Size: 701 1572 2852 Fragments arranged by size: 2852 1572 701 ApaI G_GGCC'C Cuts at: 2185 2185 Size: 5125 ApaLI G'TGCA_C Cuts at: 3182 4338 3182 Size: 1156 3969 ApoI r'AATT_y Cuts at: 1493 2434 2660 2812 4921 1493 Size: 941 226 152 2109 1697 Fragments arranged by size: 2109 1697 941 226 152 AseI AT'TA_AT Cuts at: 160 1032 3679 160 Size: 872 2647 1606 Fragments arranged by size: 2647 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5125 AvaI C'yCGr_G Cuts at: 1870 1943 2184 2450 1870 Size: 73 241 266 4545 Fragments arranged by size: 4545 266 241 73 BamHI G'GATC_C Cuts at: 2050 2373 2455 2822 2050 Size: 323 82 367 4353 Fragments arranged by size: 4353 367 323 82 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2446 3901 618 Size: 325 217 627 659 1455 1842 Fragments arranged by size: 1842 1455 659 627 325 217 BanII G_rGCy'C Cuts at: 1391 2025 2185 2444 1391 Size: 634 160 259 4072 Fragments arranged by size: 4072 634 259 160 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2135 2398 928 Size: 971 236 263 3655 Fragments arranged by size: 3655 971 263 236 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3316 3350 1903 Size: 34 1379 34 3678 Fragments arranged by size: 3678 1379 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3017 4227 3017 Size: 1210 3915 BclI T'GATC_A Cuts at: 1548 1548 Size: 5125 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2150 3736 136 Size: 107 122 71 631 605 478 1586 1525 Fragments arranged by size: 1586 1525 631 605 478 122 107 71 BglII A'GATC_T Cuts at: 2208 5120 2208 Size: 2912 2213 BlpI GC'TnA_GC Cuts at: 2542 2542 Size: 5125 Bme1580I G_kGCm'C Cuts at: 1790 2185 3186 4342 1790 Size: 395 1001 1156 2573 Fragments arranged by size: 2573 1156 1001 395 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5125 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2856 3814 442 Size: 416 53 83 399 24 1439 958 1753 Fragments arranged by size: 1753 1439 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5125 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2357 3785 1239 Size: 1118 1428 2579 Fragments arranged by size: 2579 1428 1118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3250 4115 4413 3250 Size: 865 298 3962 Fragments arranged by size: 3962 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2095 2339 3788 882 Size: 617 596 244 1449 2219 Fragments arranged by size: 2219 1449 617 596 244 BsaAI yAC'GTr Cuts at: 493 1204 2916 493 Size: 711 1712 2702 Fragments arranged by size: 2702 1712 711 BsaBI GATnn'nnATC Cuts at: 5119 5119 Size: 5125 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2932 3314 3949 275 Size: 53 83 186 936 1399 382 635 1451 Fragments arranged by size: 1451 1399 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3558 3956 4230 4377 4788 3558 Size: 398 274 147 411 3895 Fragments arranged by size: 3895 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4900 Fragments arranged by size: 4900 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5125 BseYI C'CCAG_C Cuts at: 2084 2145 4328 4824 2084 Size: 61 2183 496 2385 Fragments arranged by size: 2385 2183 496 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4552 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2575 3336 4364 1540 Size: 275 186 574 761 1028 2301 Fragments arranged by size: 2301 1028 761 574 275 186 BsiHKAI G_wGCw'C Cuts at: 2204 3186 3271 4342 2204 Size: 982 85 1071 2987 Fragments arranged by size: 2987 1071 982 85 BsmI GAATG_Cn' Cuts at: 2636 2729 2636 Size: 93 5032 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2185 2204 2444 3186 3271 4342 Size: 399 235 160 19 240 742 85 1071 Cuts at: 4342 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 240 235 160 85 19 BspEI T'CCGG_A Cuts at: 4788 4788 Size: 5125 BspHI T'CATG_A Cuts at: 3014 4744 3014 Size: 1730 3395 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2284 2480 844 Size: 1440 196 3489 Fragments arranged by size: 3489 1440 196 BsrBI CCG'CTC Cuts at: 1394 1823 3012 1394 Size: 429 1189 3507 Fragments arranged by size: 3507 1189 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3620 3794 65 Size: 1183 2372 174 1396 Fragments arranged by size: 2372 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3769 3956 1700 Size: 110 1959 187 2869 Fragments arranged by size: 2869 1959 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5125 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5125 BssSI C'ACGA_G Cuts at: 3185 4197 3185 Size: 1012 4113 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5125 BstBI TT'CG_AA Cuts at: 3929 3929 Size: 5125 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5125 BstYI r'GATC_y Cuts at: 1972 2050 2208 2373 2455 2822 3207 3224 4676 Size: 78 158 165 82 367 385 17 1452 Cuts at: 4676 4762 4774 5120 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 165 158 86 82 78 17 12 Bsu36I CC'TnA_GG Cuts at: 2069 2069 Size: 5125 BtgI C'CryG_G Cuts at: 513 1065 2004 2026 2316 513 Size: 552 939 22 290 3322 Fragments arranged by size: 3322 939 552 290 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2239 487 Size: 683 56 1013 3373 Fragments arranged by size: 3373 1013 683 56 BtsI GCAGTG_nn' Cuts at: 2641 3435 3455 2641 Size: 794 20 4311 Fragments arranged by size: 4311 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5125 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5090 DraI TTT'AAA Cuts at: 2429 2429 Size: 5125 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2117 4132 809 Size: 507 801 2015 1802 Fragments arranged by size: 2015 1802 801 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 2572 3461 Size: 54 1425 50 275 18 402 340 889 Cuts at: 3461 5090 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 402 340 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2572 1537 Size: 275 760 4090 Fragments arranged by size: 4090 760 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3055 1353 Size: 1702 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3712 4086 4232 387 Size: 1064 138 2123 374 146 1280 Fragments arranged by size: 2123 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2357 2392 3188 3785 4572 4840 1239 Size: 393 725 35 796 597 787 268 1524 Fragments arranged by size: 1524 796 787 725 597 393 268 35 EcoO109I rG'GnC_Cy Cuts at: 2181 2286 2181 Size: 105 5020 EcoRI G'AATT_C Cuts at: 2434 2812 2434 Size: 378 4747 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2152 2152 Size: 5125 FspI TGC'GCA Cuts at: 3631 3631 Size: 5125 HaeII r_GCGC'y Cuts at: 1678 4272 1678 Size: 2594 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3766 3798 3840 3872 1373 Size: 32 135 32 2194 32 42 32 2626 Fragments arranged by size: 2626 2194 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2122 2469 2715 669 Size: 988 281 184 347 246 3079 Fragments arranged by size: 3079 988 347 281 246 184 HindIII A'AGCT_T Cuts at: 748 748 Size: 5125 HpaI GTT'AAC Cuts at: 2715 2715 Size: 5125 KpnI G_GTAC'C Cuts at: 2450 2450 Size: 5125 MfeI C'AATT_G Cuts at: 2724 2724 Size: 5125 MluI A'CGCG_T Cuts at: 2196 5001 2196 Size: 2805 2320 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4239 4423 1305 Size: 2934 184 2007 Fragments arranged by size: 2934 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2234 10 Size: 54 1425 343 402 2901 Fragments arranged by size: 2901 1425 402 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3083 3442 3601 518 Size: 648 1917 359 159 2042 Fragments arranged by size: 2042 1917 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2028 2149 2157 2266 2541 3218 4366 4611 Size: 422 121 8 109 275 677 1148 245 Cuts at: 4611 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 422 275 245 121 109 8 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3634 Fragments arranged by size: 3634 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5125 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5125 NotI GC'GGCC_GC Cuts at: 2572 2572 Size: 5125 NspI r_CATG'y Cuts at: 1132 1229 2483 4028 1132 Size: 97 1254 1545 2229 Fragments arranged by size: 2229 1545 1254 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5125 PciI A'CATG_T Cuts at: 4024 4024 Size: 5125 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5125 PmeI GTTT'AAAC Cuts at: 2429 2429 Size: 5125 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2186 2218 3194 3226 4707 4739 2186 Size: 32 976 32 1481 32 2572 Fragments arranged by size: 2572 1481 976 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2286 2286 Size: 5125 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5125 PsiI TTA'TAA Cuts at: 2695 2695 Size: 5125 PspOMI G'GGCC_C Cuts at: 2181 2181 Size: 5125 PstI C_TGCA'G Cuts at: 830 2265 2271 2477 830 Size: 1435 6 206 3478 Fragments arranged by size: 3478 1435 206 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5125 PvuII CAG'CTG Cuts at: 2149 2266 2149 Size: 117 5008 SacII CC_GC'GG Cuts at: 2029 2029 Size: 5125 SalI G'TCGA_C Cuts at: 1936 2467 1936 Size: 531 4594 SbfI CC_TGCA'GG Cuts at: 2477 2477 Size: 5125 ScaI AGT'ACT Cuts at: 3373 5085 3373 Size: 1712 3413 SfcI C'TryA_G Cuts at: 826 1045 2261 2267 2473 3608 4289 4480 826 Size: 219 1216 6 206 1135 681 191 1471 Fragments arranged by size: 1471 1216 1135 681 219 206 191 6 SfiI GGCCn_nnn'nGGCC Cuts at: 2150 2150 Size: 5125 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5125 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5125 SmaI CCC'GGG Cuts at: 1872 2186 2452 1872 Size: 314 266 4545 Fragments arranged by size: 4545 314 266 SmlI C'TyrA_G Cuts at: 820 1017 1943 3229 4130 4392 820 Size: 197 926 1286 901 262 1553 Fragments arranged by size: 1553 1286 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2916 493 Size: 2423 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5125 SphI G_CATG'C Cuts at: 1132 2483 1132 Size: 1351 3774 SspI AAT'ATT Cuts at: 5 52 3049 5 Size: 47 2997 2081 Fragments arranged by size: 2997 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5125 StyI C'CwwG_G Cuts at: 513 1065 2004 2421 2564 513 Size: 552 939 417 143 3074 Fragments arranged by size: 3074 939 552 417 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2363 2434 3152 3169 3322 3507 3952 Size: 1255 783 71 718 17 153 185 445 Cuts at: 3952 325 Size: 1498 Fragments arranged by size: 1498 1255 783 718 445 185 153 71 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3371 5083 96 Size: 274 80 33 51 1443 1394 1712 138 Fragments arranged by size: 1712 1443 1394 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5085 5117 5085 Size: 32 5093 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5125 XbaI T'CTAG_A Cuts at: 2461 2461 Size: 5125 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5125 XmaI C'CCGG_G Cuts at: 1870 2184 2450 1870 Size: 314 266 4545 Fragments arranged by size: 4545 314 266 XmnI GAAnn'nnTTC Cuts at: 3254 3254 Size: 5125 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2933 276 Size: 53 83 186 936 1399 2468 Fragments arranged by size: 2468 1399 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfoI SrfI SwaI XcmI