(Linear) (Six-base) MAPSORT of: KIEE5165.seq Check: 5537 from: 1 to: 420 KIEE5165.orf With 182 enzymes: SgfI * January 29, 2014 11:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 279 420 Size: 279 141 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 387 420 Size: 387 33 AflIII A'CryG_T Cuts at: 0 191 420 Size: 191 229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 311 420 Size: 311 109 AlwNI CAG_nnn'CTG Cuts at: 0 162 420 Size: 162 258 ApaI G_GGCC'C Cuts at: 0 180 420 Size: 180 240 AvaI C'yCGr_G Cuts at: 0 179 420 Size: 179 241 BamHI G'GATC_C Cuts at: 0 45 368 420 Size: 45 323 52 Fragments arranged by size: 323 52 45 BanII G_rGCy'C Cuts at: 0 20 180 420 Size: 20 160 240 Fragments arranged by size: 240 160 20 BbsI GAAGACnn'nnnn_ Cuts at: 0 130 393 420 Size: 130 263 27 Fragments arranged by size: 263 130 27 BglI GCCn_nnn'nGGC Cuts at: 0 145 420 Size: 145 275 BglII A'GATC_T Cuts at: 0 203 420 Size: 203 217 Bme1580I G_kGCm'C Cuts at: 0 180 420 Size: 180 240 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 352 420 Size: 352 68 BsaI GGTCTCn'nnnn_ Cuts at: 0 90 334 420 Size: 90 244 86 Fragments arranged by size: 244 90 86 BseYI C'CCAG_C Cuts at: 0 79 140 420 Size: 79 61 280 Fragments arranged by size: 280 79 61 BsiHKAI G_wGCw'C Cuts at: 0 199 420 Size: 199 221 Bsp1286I G_dGCh'C Cuts at: 0 20 180 199 420 Size: 20 160 19 221 Fragments arranged by size: 221 160 20 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 279 420 Size: 279 141 BstYI r'GATC_y Cuts at: 0 45 203 368 420 Size: 45 158 165 52 Fragments arranged by size: 165 158 52 45 Bsu36I CC'TnA_GG Cuts at: 0 64 420 Size: 64 356 BtgI C'CryG_G Cuts at: 0 21 311 420 Size: 21 290 109 Fragments arranged by size: 290 109 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 234 420 Size: 234 186 DrdI GACnn_nn'nnGTC Cuts at: 0 112 420 Size: 112 308 EaeI y'GGCC_r Cuts at: 0 227 420 Size: 227 193 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 352 387 420 Size: 352 35 33 Fragments arranged by size: 352 35 33 EcoO109I rG'GnC_Cy Cuts at: 0 176 281 420 Size: 176 105 139 Fragments arranged by size: 176 139 105 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 147 420 Size: 147 273 HincII GTy'rAC Cuts at: 0 117 420 Size: 117 303 MluI A'CGCG_T Cuts at: 0 191 420 Size: 191 229 MscI TGG'CCA Cuts at: 0 229 420 Size: 229 191 MspA1I CmG'CkG Cuts at: 0 23 144 152 261 420 Size: 23 121 8 109 159 Fragments arranged by size: 159 121 109 23 8 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 181 213 420 Size: 181 32 207 Fragments arranged by size: 207 181 32 PpuMI rG'GwC_Cy Cuts at: 0 281 420 Size: 281 139 PspOMI G'GGCC_C Cuts at: 0 176 420 Size: 176 244 PstI C_TGCA'G Cuts at: 0 260 266 420 Size: 260 6 154 Fragments arranged by size: 260 154 6 PvuII CAG'CTG Cuts at: 0 144 261 420 Size: 144 117 159 Fragments arranged by size: 159 144 117 SacII CC_GC'GG Cuts at: 0 24 420 Size: 24 396 SfcI C'TryA_G Cuts at: 0 256 262 420 Size: 256 6 158 Fragments arranged by size: 256 158 6 SfiI GGCCn_nnn'nGGCC Cuts at: 0 145 420 Size: 145 275 SmaI CCC'GGG Cuts at: 0 181 420 Size: 181 239 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 358 420 Size: 358 62 XmaI C'CCGG_G Cuts at: 0 179 420 Size: 179 241 Enzymes that do cut: AarI AcuI AflIII AlfI AlwNI ApaI AvaI BamHI BanII BbsI BglI BglII Bme1580I BpmI BsaI BseYI BsiHKAI Bsp1286I BspMI BstYI Bsu36I BtgI BtgZI DrdI EaeI Eco57MI EcoO109I FalI HincII MluI MscI MspA1I PpiI PpuMI PspOMI PstI PvuII SacII SfcI SfiI SmaI TaqII XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtsI ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI MfeI MmeI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI