################################## # NO CUT ENZYMES ( 223 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AccI 22 GTAGAC GT^[AC][GT]AC AflIII 108 ACATGT A^C[GA][CT]GT AluI 171 AGCT AG^CT BbvI 15 GCAGC GCAGC(8/12) BfaI 178 CTAG C^TAG BsiYI 71 CCGAAGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 71 CCGAAGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 202 GAGAC GTCTC(1/5) BspLU11I 108 ACATGT A^CATGT BsrFI 18 GCCGGT [GA]^CCGG[CT] BsrI 77 ACTGG ACTGG(1/-1) Cac8I 82 GCTGGC GC[ACGT]^[ACGT]GC Cfr10I 18 GCCGGT [GA]^CCGG[CT] EarI 58 GAAGAG CTCTTC(1/4) Fnu4HI 15 GCAGC GC^[ACGT]GC HapII 19 CCGG C^CGG HpaII 19 CCGG C^CGG ItaI 15 GCAGC GC^[ACGT]GC Ksp632I 58 GAAGAG CTCTTC(1/4) MaeI 178 CTAG C^TAG MaeIII 165 GTTAC ^GT[ACGT]AC MspI 19 CCGG C^CGG NspI 108 ACATGT [GA]CATG^[CT] PshAI 25 GACTTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfaNI 103 GATGC GCATC(5/9) TseI 15 GCAGC G^C[AT]GC Tsp509I 124 AATT ^AATT ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### BbsI 43 GAAGAC GAAGAC(2/6) 73 GAAGAC BpuAI 43 GAAGAC GAAGAC(2/6) 73 GAAGAC BstF5I 129 GGATG GGATG(2/0) 147 GGATG DdeI 27 CTTAG C^T[ACGT]AG 87 CTTAG FokI 129 GGATG GGATG(9/13) 147 GGATG NlaIII 109 CATG CATG^ 187 CATG ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MboII MnlI