(Circular) (Six-base) MAPSORT of: pFN21AE5147.seq Check: 6537 from: 1 to: 4915 pFN21AE5147 4915 bp With 182 enzymes: SgfI * January 30, 2014 17:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2725 278 Size: 53 83 186 936 1189 2468 Fragments arranged by size: 2468 1189 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2028 2258 1937 Size: 91 230 4594 Fragments arranged by size: 4594 230 91 Acc65I G'GTAC_C Cuts at: 2236 2236 Size: 4915 AclI AA'CG_TT Cuts at: 1563 3042 3415 1563 Size: 1479 373 3063 Fragments arranged by size: 3063 1479 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2196 2978 4362 4630 1632 Size: 564 782 1384 268 1917 Fragments arranged by size: 1917 1384 782 564 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4718 AflIII A'CryG_T Cuts at: 1203 2113 3814 4791 1203 Size: 910 1701 977 1327 Fragments arranged by size: 1701 1327 977 910 AgeI A'CCGG_T Cuts at: 3746 3746 Size: 4915 AhdI GACnn_n'nnGTC Cuts at: 3644 3644 Size: 4915 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4915 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4915 AlwNI CAG_nnn'CTG Cuts at: 2658 4230 2658 Size: 1572 3343 ApaLI G'TGCA_C Cuts at: 2972 4128 2972 Size: 1156 3759 ApoI r'AATT_y Cuts at: 1493 2224 2450 2602 4711 1493 Size: 731 226 152 2109 1697 Fragments arranged by size: 2109 1697 731 226 152 AseI AT'TA_AT Cuts at: 160 1032 3469 160 Size: 872 2437 1606 Fragments arranged by size: 2437 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4915 AvaI C'yCGr_G Cuts at: 1870 1943 2240 1870 Size: 73 297 4545 Fragments arranged by size: 4545 297 73 BamHI G'GATC_C Cuts at: 2245 2612 2245 Size: 367 4548 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2236 3691 618 Size: 325 217 627 449 1455 1842 Fragments arranged by size: 1842 1455 627 449 325 217 BanII G_rGCy'C Cuts at: 1391 2234 1391 Size: 843 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2055 2085 928 Size: 971 156 30 3758 Fragments arranged by size: 3758 971 156 30 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3106 3140 1903 Size: 34 1169 34 3678 Fragments arranged by size: 3678 1169 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2807 4017 2807 Size: 1210 3705 BclI T'GATC_A Cuts at: 1548 1548 Size: 4915 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3526 136 Size: 107 122 71 631 605 1854 1525 Fragments arranged by size: 1854 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4910 4910 Size: 4915 BlpI GC'TnA_GC Cuts at: 2332 2332 Size: 4915 Bme1580I G_kGCm'C Cuts at: 1790 2976 4132 1790 Size: 1186 1156 2573 Fragments arranged by size: 2573 1186 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4915 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2092 2646 3604 Size: 416 53 83 399 24 675 554 958 Cuts at: 3604 442 Size: 1753 Fragments arranged by size: 1753 958 675 554 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4915 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3575 1239 Size: 2336 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3040 3905 4203 3040 Size: 865 298 3752 Fragments arranged by size: 3752 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3578 882 Size: 617 2079 2219 Fragments arranged by size: 2219 2079 617 BsaAI yAC'GTr Cuts at: 493 1204 2706 493 Size: 711 1502 2702 Fragments arranged by size: 2702 1502 711 BsaBI GATnn'nnATC Cuts at: 4909 4909 Size: 4915 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2722 3104 3739 275 Size: 53 83 186 936 1189 382 635 1451 Fragments arranged by size: 1451 1189 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3348 3746 4020 4167 4578 3348 Size: 398 274 147 411 3685 Fragments arranged by size: 3685 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4690 Fragments arranged by size: 4690 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4915 BseYI C'CCAG_C Cuts at: 4118 4614 4118 Size: 496 4419 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4342 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2365 3126 4154 1540 Size: 275 186 364 761 1028 2301 Fragments arranged by size: 2301 1028 761 364 275 186 BsiHKAI G_wGCw'C Cuts at: 2976 3061 4132 2976 Size: 85 1071 3759 Fragments arranged by size: 3759 1071 85 BsmI GAATG_Cn' Cuts at: 2426 2519 2426 Size: 93 4822 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2234 2976 3061 4132 1391 Size: 399 444 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 444 399 85 BspEI T'CCGG_A Cuts at: 4578 4578 Size: 4915 BspHI T'CATG_A Cuts at: 2804 4534 2804 Size: 1730 3185 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2270 844 Size: 1426 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2802 1394 Size: 429 979 3507 Fragments arranged by size: 3507 979 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3410 3584 65 Size: 1183 2162 174 1396 Fragments arranged by size: 2162 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2023 3559 3746 1700 Size: 110 213 1536 187 2869 Fragments arranged by size: 2869 1536 213 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4915 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4915 BssSI C'ACGA_G Cuts at: 2975 3987 2975 Size: 1012 3903 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4915 BstBI TT'CG_AA Cuts at: 3719 3719 Size: 4915 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4915 BstYI r'GATC_y Cuts at: 1972 2245 2612 2997 3014 4466 4552 4564 4910 Size: 273 367 385 17 1452 86 12 346 Cuts at: 4910 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 273 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4363 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4176 Fragments arranged by size: 4176 683 56 BtsI GCAGTG_nn' Cuts at: 2431 3225 3245 2431 Size: 794 20 4101 Fragments arranged by size: 4101 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4915 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4880 DraI TTT'AAA Cuts at: 2219 2219 Size: 4915 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3922 809 Size: 507 2606 1802 Fragments arranged by size: 2606 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2362 3251 4880 Size: 54 1425 50 275 18 532 889 1629 Cuts at: 4880 8 Size: 43 Fragments arranged by size: 1629 1425 889 532 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2362 1537 Size: 275 550 4090 Fragments arranged by size: 4090 550 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 2845 1353 Size: 705 787 3423 Fragments arranged by size: 3423 787 705 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3502 3876 4022 387 Size: 1064 138 1913 374 146 1280 Fragments arranged by size: 1913 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2196 2978 3575 4362 4630 1239 Size: 393 564 782 597 787 268 1524 Fragments arranged by size: 1524 787 782 597 564 393 268 EcoRI G'AATT_C Cuts at: 2224 2602 2224 Size: 378 4537 FspI TGC'GCA Cuts at: 3421 3421 Size: 4915 HaeII r_GCGC'y Cuts at: 1678 4062 1678 Size: 2384 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2144 2176 3556 3588 3630 Size: 32 135 32 572 32 1380 32 42 Cuts at: 3630 3662 1373 Size: 32 2626 Fragments arranged by size: 2626 1380 572 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2259 2505 669 Size: 988 281 321 246 3079 Fragments arranged by size: 3079 988 321 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4915 HpaI GTT'AAC Cuts at: 2505 2505 Size: 4915 KpnI G_GTAC'C Cuts at: 2240 2240 Size: 4915 MfeI C'AATT_G Cuts at: 2514 2514 Size: 4915 MluI A'CGCG_T Cuts at: 4791 4791 Size: 4915 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4029 4213 1305 Size: 2724 184 2007 Fragments arranged by size: 2724 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3093 Fragments arranged by size: 3093 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2873 3232 3391 518 Size: 648 1707 359 159 2042 Fragments arranged by size: 2042 1707 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2331 3008 4156 4401 1606 Size: 725 677 1148 245 2120 Fragments arranged by size: 2120 1148 725 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4363 NdeI CA'TA_TG Cuts at: 387 387 Size: 4915 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4915 NotI GC'GGCC_GC Cuts at: 2362 2362 Size: 4915 NspI r_CATG'y Cuts at: 1132 1229 2117 2273 3818 1132 Size: 97 888 156 1545 2229 Fragments arranged by size: 2229 1545 888 156 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4915 PciI A'CATG_T Cuts at: 2113 3814 2113 Size: 1701 3214 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4915 PmeI GTTT'AAAC Cuts at: 2219 2219 Size: 4915 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2984 3016 4497 4529 2984 Size: 32 1481 32 3370 Fragments arranged by size: 3370 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2034 1613 Size: 421 4494 PsiI TTA'TAA Cuts at: 2199 2485 2199 Size: 286 4629 PstI C_TGCA'G Cuts at: 830 2267 830 Size: 1437 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4915 SalI G'TCGA_C Cuts at: 1936 2257 1936 Size: 321 4594 SbfI CC_TGCA'GG Cuts at: 2267 2267 Size: 4915 ScaI AGT'ACT Cuts at: 3163 4875 3163 Size: 1712 3203 SfcI C'TryA_G Cuts at: 826 1045 2263 3398 4079 4270 826 Size: 219 1218 1135 681 191 1471 Fragments arranged by size: 1471 1218 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4915 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4915 SmaI CCC'GGG Cuts at: 1872 2242 1872 Size: 370 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3019 3920 4182 820 Size: 197 926 1076 901 262 1553 Fragments arranged by size: 1553 1076 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2706 493 Size: 2213 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4915 SphI G_CATG'C Cuts at: 1132 2273 1132 Size: 1141 3774 SspI AAT'ATT Cuts at: 5 52 2839 5 Size: 47 2787 2081 Fragments arranged by size: 2787 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4915 StyI C'CwwG_G Cuts at: 513 1065 2354 513 Size: 552 1289 3074 Fragments arranged by size: 3074 1289 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2942 2959 3112 3297 3742 325 Size: 1255 1362 17 153 185 445 1498 Fragments arranged by size: 1498 1362 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3161 4873 96 Size: 274 80 33 51 1443 1184 1712 138 Fragments arranged by size: 1712 1443 1184 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4875 4907 4875 Size: 32 4883 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4915 XbaI T'CTAG_A Cuts at: 2251 2251 Size: 4915 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4915 XmaI C'CCGG_G Cuts at: 1870 2240 1870 Size: 370 4545 XmnI GAAnn'nnTTC Cuts at: 3044 3044 Size: 4915 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2723 276 Size: 53 83 186 936 1189 2468 Fragments arranged by size: 2468 1189 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI