(Linear) (Six-base) MAPSORT of: KIBB3407.seq Check: 3989 from: 1 to: 3840 KIBB3407.orf With 182 enzymes: SgfI * October 14, 2014 11:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3685 3840 Size: 3685 155 AatII G_ACGT'C Cuts at: 0 1790 3840 Size: 1790 2050 AccI GT'mk_AC Cuts at: 0 1768 2073 3840 Size: 1768 305 1767 Fragments arranged by size: 1768 1767 305 Acc65I G'GTAC_C Cuts at: 0 2775 3840 Size: 2775 1065 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 782 1179 1264 2299 2714 3142 3840 Size: 782 397 85 1035 415 428 698 Fragments arranged by size: 1035 782 698 428 415 397 85 AflIII A'CryG_T Cuts at: 0 625 3840 Size: 625 3215 AleI CACnn'nnGTG Cuts at: 0 1747 3840 Size: 1747 2093 AlwNI CAG_nnn'CTG Cuts at: 0 688 760 1289 3165 3195 3218 3246 3777 Size: 688 72 529 1876 30 23 28 531 Cuts at: 3777 3780 3840 Size: 3 60 Fragments arranged by size: 1876 688 531 529 72 60 30 28 23 3 ApaI G_GGCC'C Cuts at: 0 1587 3840 Size: 1587 2253 ApaLI G'TGCA_C Cuts at: 0 493 3840 Size: 493 3347 ApoI r'AATT_y Cuts at: 0 249 837 1170 1177 1555 2109 3840 Size: 249 588 333 7 378 554 1731 Fragments arranged by size: 1731 588 554 378 333 249 7 AseI AT'TA_AT Cuts at: 0 545 1015 3840 Size: 545 470 2825 Fragments arranged by size: 2825 545 470 BanI G'GyrC_C Cuts at: 0 407 2775 2950 3840 Size: 407 2368 175 890 Fragments arranged by size: 2368 890 407 175 BanII G_rGCy'C Cuts at: 0 1587 3359 3840 Size: 1587 1772 481 Fragments arranged by size: 1772 1587 481 BbsI GAAGACnn'nnnn_ Cuts at: 0 332 429 1952 3840 Size: 332 97 1523 1888 Fragments arranged by size: 1888 1523 332 97 BciVI GTATCCnnnnn_n' Cuts at: 0 3612 3840 Size: 3612 228 BclI T'GATC_A Cuts at: 0 3029 3840 Size: 3029 811 BfrBI ATG'CAT Cuts at: 0 922 3840 Size: 922 2918 BglII A'GATC_T Cuts at: 0 258 1223 3840 Size: 258 965 2617 Fragments arranged by size: 2617 965 258 Bme1580I G_kGCm'C Cuts at: 0 497 1148 1587 1632 3567 3840 Size: 497 651 439 45 1935 273 Fragments arranged by size: 1935 651 497 439 273 45 BmgBI CAC'GTC Cuts at: 0 1673 3608 3840 Size: 1673 1935 232 Fragments arranged by size: 1935 1673 232 BmrI ACTGGGnnnn_n' Cuts at: 0 614 1581 3145 3840 Size: 614 967 1564 695 Fragments arranged by size: 1564 967 695 614 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2365 3840 Size: 2365 1475 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 770 2063 2360 2453 3193 3268 3840 Size: 340 430 1293 297 93 740 75 572 Fragments arranged by size: 1293 740 572 430 340 297 93 75 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2453 3840 Size: 2453 1387 BsaAI yAC'GTr Cuts at: 0 628 1627 3840 Size: 628 999 2213 Fragments arranged by size: 2213 999 628 BsaHI Gr'CG_yC Cuts at: 0 1787 3840 Size: 1787 2053 BsaWI w'CCGG_w Cuts at: 0 2870 3141 3840 Size: 2870 271 699 Fragments arranged by size: 2870 699 271 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1802 1832 2001 2031 2728 2758 3840 Size: 1802 30 169 30 697 30 1082 Fragments arranged by size: 1802 1082 697 169 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 41 1091 3840 Size: 41 1050 2749 Fragments arranged by size: 2749 1050 41 BseYI C'CCAG_C Cuts at: 0 367 2677 3840 Size: 367 2310 1163 Fragments arranged by size: 2310 1163 367 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1270 3840 Size: 1270 2570 BsiEI CG_ry'CG Cuts at: 0 3142 3840 Size: 3142 698 BsiHKAI G_wGCw'C Cuts at: 0 497 1303 3232 3333 3840 Size: 497 806 1929 101 507 Fragments arranged by size: 1929 806 507 497 101 BsmI GAATG_Cn' Cuts at: 0 3720 3840 Size: 3720 120 Bsp1286I G_dGCh'C Cuts at: 0 497 1148 1303 1587 1632 3232 3333 3359 Size: 497 651 155 284 45 1600 101 26 Cuts at: 3359 3567 3840 Size: 208 273 Fragments arranged by size: 1600 651 497 284 273 208 155 101 45 26 BspHI T'CATG_A Cuts at: 0 200 311 1538 1833 1844 2903 3026 3840 Size: 200 111 1227 295 11 1059 123 814 Fragments arranged by size: 1227 1059 814 295 200 123 111 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2763 3685 3840 Size: 2763 922 155 Fragments arranged by size: 2763 922 155 BsrBI CCG'CTC Cuts at: 0 3252 3840 Size: 3252 588 BsrDI GCAATG_nn' Cuts at: 0 27 1619 2600 2846 3377 3389 3548 3840 Size: 27 1592 981 246 531 12 159 292 Fragments arranged by size: 1592 981 531 292 246 159 27 12 BssSI C'ACGA_G Cuts at: 0 3560 3840 Size: 3560 280 BstAPI GCAn_nnn'nTGC Cuts at: 0 928 3840 Size: 928 2912 BstBI TT'CG_AA Cuts at: 0 1135 3840 Size: 1135 2705 BstXI CCAn_nnnn'nTGG Cuts at: 0 187 1594 2961 3840 Size: 187 1407 1367 879 Fragments arranged by size: 1407 1367 879 187 BstYI r'GATC_y Cuts at: 0 3 258 1223 1649 1975 2345 3840 Size: 3 255 965 426 326 370 1495 Fragments arranged by size: 1495 965 426 370 326 255 3 BstZ17I GTA'TAC Cuts at: 0 2074 3840 Size: 2074 1766 BtgI C'CryG_G Cuts at: 0 180 1487 2016 2954 2960 3840 Size: 180 1307 529 938 6 880 Fragments arranged by size: 1307 938 880 529 180 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1776 3840 Size: 1776 2064 BtsI GCAGTG_nn' Cuts at: 0 1706 3219 3478 3840 Size: 1706 1513 259 362 Fragments arranged by size: 1706 1513 362 259 DraI TTT'AAA Cuts at: 0 51 3840 Size: 51 3789 EaeI y'GGCC_r Cuts at: 0 656 1470 2957 3534 3840 Size: 656 814 1487 577 306 Fragments arranged by size: 1487 814 656 577 306 EarI CTCTTCn'nnn_ Cuts at: 0 758 1132 1291 3220 3422 3840 Size: 758 374 159 1929 202 418 Fragments arranged by size: 1929 758 418 374 202 159 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 770 782 1179 1264 2063 2299 2360 Size: 340 430 12 397 85 799 236 61 Cuts at: 2360 2453 2714 3142 3193 3268 3840 Size: 93 261 428 51 75 572 Fragments arranged by size: 799 572 430 428 397 340 261 236 93 85 75 61 51 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 2091 3840 Size: 2091 1749 EcoO109I rG'GnC_Cy Cuts at: 0 1233 1583 3840 Size: 1233 350 2257 Fragments arranged by size: 2257 1233 350 EcoRI G'AATT_C Cuts at: 0 1177 3840 Size: 1177 2663 EcoRV GAT'ATC Cuts at: 0 231 291 3840 Size: 231 60 3549 Fragments arranged by size: 3549 231 60 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 60 3301 3840 Size: 60 3241 539 Fragments arranged by size: 3241 539 60 HaeII r_GCGC'y Cuts at: 0 2678 3837 3840 Size: 2678 1159 3 Fragments arranged by size: 2678 1159 3 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 182 214 250 282 520 552 977 1009 Size: 182 32 36 32 238 32 425 32 Cuts at: 1009 1657 1689 1743 1775 1960 1992 2372 2404 Size: 648 32 54 32 185 32 380 32 Cuts at: 2404 3131 3163 3183 3215 3840 Size: 727 32 20 32 625 Fragments arranged by size: 727 648 625 425 380 238 185 182 54 36 32 32 32 32 32 32 32 32 32 32 20 HindIII A'AGCT_T Cuts at: 0 2041 3840 Size: 2041 1799 KpnI G_GTAC'C Cuts at: 0 2779 3840 Size: 2779 1061 MfeI C'AATT_G Cuts at: 0 2588 3840 Size: 2588 1252 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 613 1014 1257 1551 1717 3840 Size: 613 401 243 294 166 2123 Fragments arranged by size: 2123 613 401 294 243 166 MscI TGG'CCA Cuts at: 0 658 3536 3840 Size: 658 2878 304 Fragments arranged by size: 2878 658 304 MslI CAynn'nnrTG Cuts at: 0 405 1411 1747 2756 2959 3025 3151 3840 Size: 405 1006 336 1009 203 66 126 689 Fragments arranged by size: 1009 1006 689 405 336 203 126 66 MspA1I CmG'CkG Cuts at: 0 418 685 1314 1921 2938 3777 3840 Size: 418 267 629 607 1017 839 63 Fragments arranged by size: 1017 839 629 607 418 267 63 NcoI C'CATG_G Cuts at: 0 180 1487 2954 3840 Size: 180 1307 1467 886 Fragments arranged by size: 1467 1307 886 180 NdeI CA'TA_TG Cuts at: 0 2753 3840 Size: 2753 1087 NsiI A_TGCA'T Cuts at: 0 924 3840 Size: 924 2916 NspI r_CATG'y Cuts at: 0 3466 3840 Size: 3466 374 PflMI CCAn_nnn'nTGG Cuts at: 0 3195 3246 3840 Size: 3195 51 594 Fragments arranged by size: 3195 594 51 PfoI T'CCnGG_A Cuts at: 0 3245 3840 Size: 3245 595 PmlI CAC'GTG Cuts at: 0 628 1627 3840 Size: 628 999 2213 Fragments arranged by size: 2213 999 628 PsiI TTA'TAA Cuts at: 0 2158 3840 Size: 2158 1682 PspOMI G'GGCC_C Cuts at: 0 1583 3840 Size: 1583 2257 PstI C_TGCA'G Cuts at: 0 3376 3840 Size: 3376 464 PvuII CAG'CTG Cuts at: 0 418 685 1314 1921 2938 3777 3840 Size: 418 267 629 607 1017 839 63 Fragments arranged by size: 1017 839 629 607 418 267 63 SapI GCTCTTCn'nnn_ Cuts at: 0 1291 3220 3840 Size: 1291 1929 620 Fragments arranged by size: 1929 1291 620 ScaI AGT'ACT Cuts at: 0 992 3840 Size: 992 2848 SexAI A'CCwGG_T Cuts at: 0 334 3840 Size: 334 3506 SfcI C'TryA_G Cuts at: 0 893 1769 2483 3060 3372 3840 Size: 893 876 714 577 312 468 Fragments arranged by size: 893 876 714 577 468 312 SmlI C'TyrA_G Cuts at: 0 2468 3840 Size: 2468 1372 SspI AAT'ATT Cuts at: 0 888 976 1161 2192 3840 Size: 888 88 185 1031 1648 Fragments arranged by size: 1648 1031 888 185 88 StuI AGG'CCT Cuts at: 0 2168 3074 3840 Size: 2168 906 766 Fragments arranged by size: 2168 906 766 StyI C'CwwG_G Cuts at: 0 180 956 1487 1679 1949 2283 2954 3266 Size: 180 776 531 192 270 334 671 312 Cuts at: 3266 3840 Size: 574 Fragments arranged by size: 776 671 574 531 334 312 270 192 180 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2848 3840 Size: 2848 992 TatI w'GTAC_w Cuts at: 0 990 3840 Size: 990 2850 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1338 1370 1802 1834 2001 2033 3685 3717 Size: 1338 32 432 32 167 32 1652 32 Cuts at: 3717 3840 Size: 123 Fragments arranged by size: 1652 1338 432 167 123 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1344 1594 1677 2058 2087 3612 3840 Size: 1344 250 83 381 29 1525 228 Fragments arranged by size: 1525 1344 381 250 228 83 29 XmnI GAAnn'nnTTC Cuts at: 0 1460 2292 3840 Size: 1460 832 1548 Fragments arranged by size: 1548 1460 832 ZraI GAC'GTC Cuts at: 0 1788 3840 Size: 1788 2052 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AflIII AleI AlwNI ApaI ApaLI ApoI AseI BanI BanII BbsI BciVI BclI BfrBI BglII Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HindIII KpnI MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PflMI PfoI PmlI PsiI PspOMI PstI PvuII SapI ScaI SexAI SfcI SmlI SspI StuI StyI TaqII TatI TstI XcmI XmnI ZraI Enzymes that do not cut: AclI AfeI AflII AgeI AhdI AlfI AloI AscI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BglI BlpI BmtI Bpu10I BsaI BsaBI BsiWI BsmBI BspEI BsrFI BsrGI BssHII BstEII Bsu36I ClaI CspCI DraIII DrdI EagI EciI EcoICRI FseI FspI FspAI HincII HpaI KasI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PpiI PpuMI PshAI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI