(Circular) (Six-base) MAPSORT of: pFN21AE2188.seq Check: 720 from: 1 to: 5974 pFN21AE2188 5974 bp With 182 enzymes: SgfI * August 20, 2012 11:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2128 2679 2993 3317 1937 Size: 191 551 314 324 4594 Fragments arranged by size: 4594 551 324 314 191 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2242 2567 2638 4037 5421 5689 1632 Size: 610 325 71 1399 1384 268 1917 Fragments arranged by size: 1917 1399 1384 610 325 268 71 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 4873 5850 1203 Size: 3670 977 1327 Fragments arranged by size: 3670 1327 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 2783 3172 1166 Size: 1617 389 3968 Fragments arranged by size: 3968 1617 389 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5974 AlwNI CAG_nnn'CTG Cuts at: 2213 2276 3717 5289 2213 Size: 63 1441 1572 2898 Fragments arranged by size: 2898 1572 1441 63 ApaI G_GGCC'C Cuts at: 2029 2868 2029 Size: 839 5135 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 3021 3283 3509 3661 5770 1493 Size: 1528 262 226 152 2109 1697 Fragments arranged by size: 2109 1697 1528 262 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 3299 1870 Size: 73 1356 4545 Fragments arranged by size: 4545 1356 73 BamHI G'GATC_C Cuts at: 3304 3671 3304 Size: 367 5607 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3295 4750 618 Size: 325 217 627 1508 1455 1842 Fragments arranged by size: 1842 1508 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2029 2051 2316 2868 3293 1391 Size: 638 22 265 552 425 4072 Fragments arranged by size: 4072 638 552 425 265 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2026 928 Size: 971 127 4876 Fragments arranged by size: 4876 971 127 BbvCI CC'TCA_GC Cuts at: 2364 2364 Size: 5974 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4165 4199 1903 Size: 34 2228 34 3678 Fragments arranged by size: 3678 2228 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 1548 Size: 5974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4585 136 Size: 107 122 71 631 605 2913 1525 Fragments arranged by size: 2913 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3162 5969 3162 Size: 2807 3167 BlpI GC'TnA_GC Cuts at: 3391 3391 Size: 5974 Bme1580I G_kGCm'C Cuts at: 1790 2029 2868 4035 5191 1790 Size: 239 839 1167 1156 2573 Fragments arranged by size: 2573 1167 1156 839 239 BmgBI CAC'GTC Cuts at: 1318 2032 1318 Size: 714 5260 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2959 3104 3705 Size: 416 53 83 399 24 1542 145 601 Cuts at: 3705 4663 442 Size: 958 1753 Fragments arranged by size: 1753 1542 958 601 416 399 145 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2372 2957 3104 3229 4634 1239 Size: 1133 585 147 125 1405 2579 Fragments arranged by size: 2579 1405 1133 585 147 125 Bpu10I CC'TnA_GC Cuts at: 2364 2946 3192 2364 Size: 582 246 5146 Fragments arranged by size: 5146 582 246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2510 2701 4099 4964 5262 2510 Size: 191 1398 865 298 3222 Fragments arranged by size: 3222 1398 865 298 191 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2620 4637 882 Size: 617 1121 2017 2219 Fragments arranged by size: 2219 2017 1121 617 BsaAI yAC'GTr Cuts at: 493 1204 2781 3765 493 Size: 711 1577 984 2702 Fragments arranged by size: 2702 1577 984 711 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2980 3781 4163 4798 Size: 53 83 186 936 1447 801 382 635 Cuts at: 4798 275 Size: 1451 Fragments arranged by size: 1451 1447 936 801 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2822 2852 3202 3232 1178 Size: 30 165 30 1419 30 350 30 3920 Fragments arranged by size: 3920 1419 350 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 2381 1186 Size: 970 225 4779 Fragments arranged by size: 4779 970 225 BseYI C'CCAG_C Cuts at: 2100 2502 2711 2751 2820 3088 5177 5673 2100 Size: 402 209 40 69 268 2089 496 2401 Fragments arranged by size: 2401 2089 496 402 268 209 69 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5401 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 2883 3424 4185 5213 1540 Size: 275 186 60 822 541 761 1028 2301 Fragments arranged by size: 2301 1028 822 761 541 275 186 60 BsiHKAI G_wGCw'C Cuts at: 2769 4035 4120 5191 2769 Size: 1266 85 1071 3552 Fragments arranged by size: 3552 1266 1071 85 BsmI GAATG_Cn' Cuts at: 3485 3578 3485 Size: 93 5881 BsmBI CGTCTCn'nnnn_ Cuts at: 2987 2987 Size: 5974 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2051 2316 2769 2868 3293 4035 Size: 399 239 22 265 453 99 425 742 Cuts at: 4035 4120 5191 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 453 425 399 265 239 99 85 22 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3863 5593 3863 Size: 1730 4244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3329 844 Size: 2485 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3243 3861 1394 Size: 429 1420 618 3507 Fragments arranged by size: 3507 1420 618 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4469 4643 65 Size: 1183 3221 174 1396 Fragments arranged by size: 3221 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4618 4805 1700 Size: 110 2808 187 2869 Fragments arranged by size: 2869 2808 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5974 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 2224 1184 Size: 1040 4934 BstYI r'GATC_y Cuts at: 1972 3162 3304 3671 4056 4073 5525 5611 5623 Size: 1190 142 367 385 17 1452 86 12 Cuts at: 5623 5969 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1190 385 367 346 142 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2406 513 Size: 552 939 402 4081 Fragments arranged by size: 4081 939 552 402 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2797 487 Size: 683 56 1571 3664 Fragments arranged by size: 3664 1571 683 56 BtsI GCAGTG_nn' Cuts at: 2476 2584 3490 4284 4304 2476 Size: 108 906 794 20 4146 Fragments arranged by size: 4146 906 794 108 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2198 2442 2622 Size: 54 1425 50 275 18 368 244 180 Cuts at: 2622 3181 3421 4310 5939 8 Size: 559 240 889 1629 43 Fragments arranged by size: 1629 1425 889 559 368 275 244 240 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2441 2774 3904 1353 Size: 1088 333 1130 3423 Fragments arranged by size: 3423 1130 1088 333 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3062 4561 4935 5081 387 Size: 1064 138 1473 1499 374 146 1280 Fragments arranged by size: 1499 1473 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2242 2372 2567 2638 2957 3104 3229 Size: 393 610 130 195 71 319 147 125 Cuts at: 3229 4037 4634 5421 5689 1239 Size: 808 597 787 268 1524 Fragments arranged by size: 1524 808 787 610 597 393 319 268 195 147 130 125 71 EcoO109I rG'GnC_Cy Cuts at: 2025 2824 2864 3213 2025 Size: 799 40 349 4786 Fragments arranged by size: 4786 799 349 40 EcoRI G'AATT_C Cuts at: 3021 3283 3661 3021 Size: 262 378 5334 Fragments arranged by size: 5334 378 262 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 5121 1678 Size: 3443 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2110 2142 2234 2266 2357 Size: 32 135 32 538 32 92 32 91 Cuts at: 2357 2389 2822 2854 4615 4647 4689 4721 1373 Size: 32 433 32 1761 32 42 32 2626 Fragments arranged by size: 2626 1761 538 433 135 92 91 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2994 3318 3564 669 Size: 988 281 1056 324 246 3079 Fragments arranged by size: 3079 1056 988 324 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5974 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5088 5272 1305 Size: 3783 184 2007 Fragments arranged by size: 3783 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2200 2444 3183 10 Size: 54 1425 343 368 244 739 2801 Fragments arranged by size: 2801 1425 739 368 343 244 54 MslI CAynn'nnrTG Cuts at: 518 1166 2783 2924 3172 3183 3268 3932 4291 Size: 648 1617 141 248 11 85 664 359 Cuts at: 4291 4450 518 Size: 159 2042 Fragments arranged by size: 2042 1617 664 648 359 248 159 141 85 11 MspA1I CmG'CkG Cuts at: 1606 2104 2506 2658 3390 4067 5215 5460 1606 Size: 498 402 152 732 677 1148 245 2120 Fragments arranged by size: 2120 1148 732 677 498 402 245 152 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4483 Fragments arranged by size: 4483 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 2077 3332 4877 1132 Size: 97 848 1255 1545 2229 Fragments arranged by size: 2229 1545 1255 848 97 PasI CC'CwG_GG Cuts at: 1348 2161 1348 Size: 813 5161 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 3017 1108 Size: 1909 4065 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PmlI CAC'GTG Cuts at: 2781 2781 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 5556 5588 4043 Size: 32 1481 32 4429 Fragments arranged by size: 4429 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2824 3213 2824 Size: 389 5585 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PspOMI G'GGCC_C Cuts at: 2025 2864 2025 Size: 839 5135 PstI C_TGCA'G Cuts at: 830 2591 3326 830 Size: 1761 735 3478 Fragments arranged by size: 3478 1761 735 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2104 2506 2658 2104 Size: 402 152 5420 Fragments arranged by size: 5420 402 152 SalI G'TCGA_C Cuts at: 1936 2992 3316 1936 Size: 1056 324 4594 Fragments arranged by size: 4594 1056 324 SapI GCTCTTCn'nnn_ Cuts at: 2774 2774 Size: 5974 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 4222 5934 4222 Size: 1712 4262 SfcI C'TryA_G Cuts at: 826 1045 2587 3322 4457 5138 5329 826 Size: 219 1542 735 1135 681 191 1471 Fragments arranged by size: 1542 1471 1135 735 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2525 2716 4078 4979 5241 820 Size: 197 926 582 191 1362 901 262 1553 Fragments arranged by size: 1553 1362 926 901 582 262 197 191 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 2911 3898 5 Size: 47 2859 987 2081 Fragments arranged by size: 2859 2081 987 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5974 StyI C'CwwG_G Cuts at: 513 1065 2004 3413 513 Size: 552 939 1409 3074 Fragments arranged by size: 3074 1409 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2094 2114 2516 2773 2869 4001 4018 Size: 1255 514 20 402 257 96 1132 17 Cuts at: 4018 4171 4356 4801 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1132 514 445 402 257 185 153 96 20 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2291 4220 5932 Size: 274 80 33 51 1443 314 1929 1712 Cuts at: 5932 96 Size: 138 Fragments arranged by size: 1929 1712 1443 314 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2801 2833 3202 3234 5934 5966 2801 Size: 32 369 32 2700 32 2809 Fragments arranged by size: 2809 2700 369 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5974 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2158 2452 2158 Size: 294 5680 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 4103 4103 Size: 5974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI