Restriction map for pFN21AE2188
 (Circular) (Six-base) MAPSORT of: pFN21AE2188.seq  Check: 720  from: 1  to: 5974



pFN21AE2188  5974 bp

 With 182 enzymes: SgfI * 

                        August 20, 2012 11:37  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3784     278 
   Size:         53      83     186     936    2248    2468

  Fragments arranged by size: 

                2468    2248     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2128    2679    2993    3317    1937 
   Size:        191     551     314     324    4594

  Fragments arranged by size: 

                4594     551     324     314     191
 
Acc65I G'GTAC_C

Cuts at:   3295    3295 
   Size:       5974
 
AclI AA'CG_TT

Cuts at:   1563    4101    4474    1563 
   Size:       2538     373    3063

  Fragments arranged by size: 

                3063    2538     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2242    2567    2638    4037    5421    5689    1632 
   Size:        610     325      71    1399    1384     268    1917

  Fragments arranged by size: 

                1917    1399    1384     610     325     268      71
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5777
 
AflIII A'CryG_T

Cuts at:   1203    4873    5850    1203 
   Size:       3670     977    1327

  Fragments arranged by size: 

                3670    1327     977
 
AgeI A'CCGG_T

Cuts at:   4805    4805 
   Size:       5974
 
AhdI GACnn_n'nnGTC

Cuts at:   4703    4703 
   Size:       5974
 
AleI CACnn'nnGTG

Cuts at:   1166    2783    3172    1166 
   Size:       1617     389    3968

  Fragments arranged by size: 

                3968    1617     389
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5974
 
AlwNI CAG_nnn'CTG

Cuts at:   2213    2276    3717    5289    2213 
   Size:         63    1441    1572    2898

  Fragments arranged by size: 

                2898    1572    1441      63
 
ApaI G_GGCC'C

Cuts at:   2029    2868    2029 
   Size:        839    5135
 
ApaLI G'TGCA_C

Cuts at:   4031    5187    4031 
   Size:       1156    4818
 
ApoI r'AATT_y

Cuts at:   1493    3021    3283    3509    3661    5770    1493 
   Size:       1528     262     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1528     262     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4528     160 
   Size:        872    3496    1606

  Fragments arranged by size: 

                3496    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5974
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3299    1870 
   Size:         73    1356    4545

  Fragments arranged by size: 

                4545    1356      73
 
BamHI G'GATC_C

Cuts at:   3304    3671    3304 
   Size:        367    5607
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3295    4750     618 
   Size:        325     217     627    1508    1455    1842

  Fragments arranged by size: 

                1842    1508    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2029    2051    2316    2868    3293    1391 
   Size:        638      22     265     552     425    4072

  Fragments arranged by size: 

                4072     638     552     425     265      22
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2026     928 
   Size:        971     127    4876

  Fragments arranged by size: 

                4876     971     127
 
BbvCI CC'TCA_GC

Cuts at:   2364    2364 
   Size:       5974
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4165    4199    1903 
   Size:         34    2228      34    3678

  Fragments arranged by size: 

                3678    2228      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3866    5076    3866 
   Size:       1210    4764
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5974
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4585     136 
   Size:        107     122      71     631     605    2913    1525

  Fragments arranged by size: 

                2913    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   3162    5969    3162 
   Size:       2807    3167
 
BlpI GC'TnA_GC

Cuts at:   3391    3391 
   Size:       5974
 
Bme1580I G_kGCm'C

Cuts at:   1790    2029    2868    4035    5191    1790 
   Size:        239     839    1167    1156    2573

  Fragments arranged by size: 

                2573    1167    1156     839     239
 
BmgBI CAC'GTC

Cuts at:   1318    2032    1318 
   Size:        714    5260
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2959    3104    3705 
   Size:        416      53      83     399      24    1542     145     601

Cuts at:   3705    4663     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1542     958     601     416     399     145      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5974
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2372    2957    3104    3229    4634    1239 
   Size:       1133     585     147     125    1405    2579

  Fragments arranged by size: 

                2579    1405    1133     585     147     125
 
Bpu10I CC'TnA_GC

Cuts at:   2364    2946    3192    2364 
   Size:        582     246    5146

  Fragments arranged by size: 

                5146     582     246
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2510    2701    4099    4964    5262    2510 
   Size:        191    1398     865     298    3222

  Fragments arranged by size: 

                3222    1398     865     298     191
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2620    4637     882 
   Size:        617    1121    2017    2219

  Fragments arranged by size: 

                2219    2017    1121     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2781    3765     493 
   Size:        711    1577     984    2702

  Fragments arranged by size: 

                2702    1577     984     711
 
BsaBI GATnn'nnATC

Cuts at:   5968    5968 
   Size:       5974
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2980    3781    4163    4798 
   Size:         53      83     186     936    1447     801     382     635

Cuts at:   4798     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1447     936     801     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4407    4805    5079    5226    5637    4407 
   Size:        398     274     147     411    4744

  Fragments arranged by size: 

                4744     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2822    2852    3202    3232    1178 
   Size:         30     165      30    1419      30     350      30    3920

  Fragments arranged by size: 

                3920    1419     350     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2156    2381    1186 
   Size:        970     225    4779

  Fragments arranged by size: 

                4779     970     225
 
BseYI C'CCAG_C

Cuts at:   2100    2502    2711    2751    2820    3088    5177    5673    2100 
   Size:        402     209      40      69     268    2089     496    2401

  Fragments arranged by size: 

                2401    2089     496     402     268     209      69      40
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5401
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2061    2883    3424    4185    5213    1540 
   Size:        275     186      60     822     541     761    1028    2301

  Fragments arranged by size: 

                2301    1028     822     761     541     275     186      60
            
 
BsiHKAI G_wGCw'C

Cuts at:   2769    4035    4120    5191    2769 
   Size:       1266      85    1071    3552

  Fragments arranged by size: 

                3552    1266    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3485    3578    3485 
   Size:         93    5881
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2987    2987 
   Size:       5974
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2029    2051    2316    2769    2868    3293    4035 
   Size:        399     239      22     265     453      99     425     742

Cuts at:   4035    4120    5191    1391 
   Size:         85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     453     425     399     265     239
                  99      85      22
 
BspEI T'CCGG_A

Cuts at:   5637    5637 
   Size:       5974
 
BspHI T'CATG_A

Cuts at:   3863    5593    3863 
   Size:       1730    4244
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3329     844 
   Size:       2485    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3243    3861    1394 
   Size:        429    1420     618    3507

  Fragments arranged by size: 

                3507    1420     618     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4469    4643      65 
   Size:       1183    3221     174    1396

  Fragments arranged by size: 

                3221    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4618    4805    1700 
   Size:        110    2808     187    2869

  Fragments arranged by size: 

                2869    2808     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5974
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5974
 
BssSI C'ACGA_G

Cuts at:   4034    5046    4034 
   Size:       1012    4962
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5974
 
BstBI TT'CG_AA

Cuts at:   4778    4778 
   Size:       5974
 
BstEII G'GTnAC_C

Cuts at:   1184    2224    1184 
   Size:       1040    4934
 
BstYI r'GATC_y

Cuts at:   1972    3162    3304    3671    4056    4073    5525    5611    5623 
   Size:       1190     142     367     385      17    1452      86      12

Cuts at:   5623    5969    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452    1190     385     367     346     142      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2406     513 
   Size:        552     939     402    4081

  Fragments arranged by size: 

                4081     939     552     402
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2797     487 
   Size:        683      56    1571    3664

  Fragments arranged by size: 

                3664    1571     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2476    2584    3490    4284    4304    2476 
   Size:        108     906     794      20    4146

  Fragments arranged by size: 

                4146     906     794     108      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5974
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5939
 
DraI TTT'AAA

Cuts at:   3278    3278 
   Size:       5974
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4981     809 
   Size:        507    3665    1802

  Fragments arranged by size: 

                3665    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2198    2442    2622 
   Size:         54    1425      50     275      18     368     244     180

Cuts at:   2622    3181    3421    4310    5939       8 
   Size:        559     240     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     559     368     275     244     240
                 180      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3421    1537 
   Size:        275    1609    4090

  Fragments arranged by size: 

                4090    1609     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2441    2774    3904    1353 
   Size:       1088     333    1130    3423

  Fragments arranged by size: 

                3423    1130    1088     333
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3062    4561    4935    5081     387 
   Size:       1064     138    1473    1499     374     146    1280

  Fragments arranged by size: 

                1499    1473    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2242    2372    2567    2638    2957    3104    3229 
   Size:        393     610     130     195      71     319     147     125

Cuts at:   3229    4037    4634    5421    5689    1239 
   Size:        808     597     787     268    1524

  Fragments arranged by size: 

                1524     808     787     610     597     393     319     268
                 195     147     130     125      71
 
EcoO109I rG'GnC_Cy

Cuts at:   2025    2824    2864    3213    2025 
   Size:        799      40     349    4786

  Fragments arranged by size: 

                4786     799     349      40
 
EcoRI G'AATT_C

Cuts at:   3021    3283    3661    3021 
   Size:        262     378    5334

  Fragments arranged by size: 

                5334     378     262
 
FspI TGC'GCA

Cuts at:   4480    4480 
   Size:       5974
 
HaeII r_GCGC'y

Cuts at:   1678    5121    1678 
   Size:       3443    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2110    2142    2234    2266    2357 
   Size:         32     135      32     538      32      92      32      91

Cuts at:   2357    2389    2822    2854    4615    4647    4689    4721    1373 
   Size:         32     433      32    1761      32      42      32    2626

  Fragments arranged by size: 

                2626    1761     538     433     135      92      91      42
                  32      32      32      32      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2994    3318    3564     669 
   Size:        988     281    1056     324     246    3079

  Fragments arranged by size: 

                3079    1056     988     324     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5974
 
HpaI GTT'AAC

Cuts at:   3564    3564 
   Size:       5974
 
KpnI G_GTAC'C

Cuts at:   3299    3299 
   Size:       5974
 
MfeI C'AATT_G

Cuts at:   3573    3573 
   Size:       5974
 
MluI A'CGCG_T

Cuts at:   5850    5850 
   Size:       5974
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5088    5272    1305 
   Size:       3783     184    2007

  Fragments arranged by size: 

                3783    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2200    2444    3183      10 
   Size:         54    1425     343     368     244     739    2801

  Fragments arranged by size: 

                2801    1425     739     368     343     244      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2783    2924    3172    3183    3268    3932    4291 
   Size:        648    1617     141     248      11      85     664     359

Cuts at:   4291    4450     518 
   Size:        159    2042

  Fragments arranged by size: 

                2042    1617     664     648     359     248     159     141
                  85      11
 
MspA1I CmG'CkG

Cuts at:   1606    2104    2506    2658    3390    4067    5215    5460    1606 
   Size:        498     402     152     732     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     732     677     498     402     245     152
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4483

  Fragments arranged by size: 

                4483     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5974
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5974
 
NotI GC'GGCC_GC

Cuts at:   3421    3421 
   Size:       5974
 
NspI r_CATG'y

Cuts at:   1132    1229    2077    3332    4877    1132 
   Size:         97     848    1255    1545    2229

  Fragments arranged by size: 

                2229    1545    1255     848      97
 
PasI CC'CwG_GG

Cuts at:   1348    2161    1348 
   Size:        813    5161
 
PciI A'CATG_T

Cuts at:   4873    4873 
   Size:       5974
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3017    1108 
   Size:       1909    4065
 
PmeI GTTT'AAAC

Cuts at:   3278    3278 
   Size:       5974
 
PmlI CAC'GTG

Cuts at:   2781    2781 
   Size:       5974
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4043    4075    5556    5588    4043 
   Size:         32    1481      32    4429

  Fragments arranged by size: 

                4429    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2824    3213    2824 
   Size:        389    5585
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5974
 
PsiI TTA'TAA

Cuts at:   3544    3544 
   Size:       5974
 
PspOMI G'GGCC_C

Cuts at:   2025    2864    2025 
   Size:        839    5135
 
PstI C_TGCA'G

Cuts at:    830    2591    3326     830 
   Size:       1761     735    3478

  Fragments arranged by size: 

                3478    1761     735
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5974
 
PvuII CAG'CTG

Cuts at:   2104    2506    2658    2104 
   Size:        402     152    5420

  Fragments arranged by size: 

                5420     402     152
 
SalI G'TCGA_C

Cuts at:   1936    2992    3316    1936 
   Size:       1056     324    4594

  Fragments arranged by size: 

                4594    1056     324
 
SapI GCTCTTCn'nnn_

Cuts at:   2774    2774 
   Size:       5974
 
SbfI CC_TGCA'GG

Cuts at:   3326    3326 
   Size:       5974
 
ScaI AGT'ACT

Cuts at:   4222    5934    4222 
   Size:       1712    4262
 
SfcI C'TryA_G

Cuts at:    826    1045    2587    3322    4457    5138    5329     826 
   Size:        219    1542     735    1135     681     191    1471

  Fragments arranged by size: 

                1542    1471    1135     735     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5974
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5974
 
SmaI CCC'GGG

Cuts at:   1872    3301    1872 
   Size:       1429    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2525    2716    4078    4979    5241     820 
   Size:        197     926     582     191    1362     901     262    1553

  Fragments arranged by size: 

                1553    1362     926     901     582     262     197     191
            
 
SnaBI TAC'GTA

Cuts at:    493    3765     493 
   Size:       3272    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5974
 
SphI G_CATG'C

Cuts at:   1132    3332    1132 
   Size:       2200    3774
 
SspI AAT'ATT

Cuts at:      5      52    2911    3898       5 
   Size:         47    2859     987    2081

  Fragments arranged by size: 

                2859    2081     987      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5974
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3413     513 
   Size:        552     939    1409    3074

  Fragments arranged by size: 

                3074    1409     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2094    2114    2516    2773    2869    4001    4018 
   Size:       1255     514      20     402     257      96    1132      17

Cuts at:   4018    4171    4356    4801     325 
   Size:        153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1132     514     445     402     257     185
                 153      96      20      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2291    4220    5932 
   Size:        274      80      33      51    1443     314    1929    1712

Cuts at:   5932      96 
   Size:        138

  Fragments arranged by size: 

                1929    1712    1443     314     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2801    2833    3202    3234    5934    5966    2801 
   Size:         32     369      32    2700      32    2809

  Fragments arranged by size: 

                2809    2700     369      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5974
 
XbaI T'CTAG_A

Cuts at:   3310    3310 
   Size:       5974
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2158    2452    2158 
   Size:        294    5680
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5974
 
XmaI C'CCGG_G

Cuts at:   1870    3299    1870 
   Size:       1429    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4103    4103 
   Size:       5974
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3782     276 
   Size:         53      83     186     936    2248    2468

  Fragments arranged by size: 

                2468    2248     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PstI     PvuI    PvuII     SalI     SapI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BfrBI
     BplI    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI    PspXI     PsrI    RsrII     SacI
    SacII    SanDI    SexAI     SfiI     SfoI     SrfI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com