################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AlwNI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI Eco81I EcoRI EcoRV EcoT22I FseI HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcyI 208 GGCGTC G[GA]^CG[CT]C AhdI 997 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 526 GTGCAC G^TGCAC Aor51HI 160 AGCGCT AGC^GCT ApaLI 526 GTGCAC G^TGCAC AspEI 997 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 665 TGCGCA TGC^GCA BamHI 863 GGATCC G^GATCC BbsI 1069 GTCTTC GAAGAC(2/6) BclI 830 TGATCA T^GATCA BglI 388 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1100 GCTCAGC GC^T[ACGT]AGC BpmI 28 CTGGAG CTGGAG(16/14) Bpu1102I 1100 GCTCAGC GC^T[ACGT]AGC BpuAI 1069 GTCTTC GAAGAC(2/6) BsaHI 208 GGCGTC G[GA]^CG[CT]C BsiEI 1223 CGGCCG CG[GA][CT]^CG BsmAI 901 GAGAC GTCTC(1/5) BspHI 641 TCATGA T^CATGA BsrDI 536 GCAATG GCAATG(2/0) BstEII 585 GGTGACC G^GT[ACGT]ACC BstPI 585 GGTGACC G^GT[ACGT]ACC BstYI 863 GGATCC [GA]^GATC[CT] CelII 1100 GCTCAGC GC^T[ACGT]AGC DraIII 287 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1157 CCATGG C^C[GA][CT]GG EagI 1223 CGGCCG C^GGCCG Eam1105I 997 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1223 CGGCCG C^GGCCG Eco47III 160 AGCGCT AGC^GCT Eco52I 1223 CGGCCG C^GGCCG EcoNI 24 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 585 GGTGACC G^GT[ACGT]ACC FauI 155 CCCGC CCCGC(4/6) FbaI 830 TGATCA T^GATCA FspI 665 TGCGCA TGC^GCA Hin1I 208 GGCGTC G[GA]^CG[CT]C MflI 863 GGATCC [GA]^GATC[CT] NcoI 1157 CCATGG C^CATGG NspI 968 ACATGC [GA]CATG^[CT] PleI 1151 GAGTC GAGTC(4/5) PpuMI 102 GGGTCCC [GA]G^G[AT]CC[CT] RcaI 641 TCATGA T^CATGA ScaI 197 AGTACT AGT^ACT SexAI 785 ACCTGGT A^CC[AT]GGT TfiI 450 GATTC G^A[AT]TC Tsp509I 835 AATT ^AATT XcmI 35 CCAACTGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 863 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BanI 548 GGTGCC G^G[CT][GA]CC 1121 GGCACC BanII 170 GAGCCC G[GA]GC[CT]^C 295 GAGCCC BsgI 331 CTGCAC GTGCAG(16/14) 625 CTGCAC BspMI 329 ACCTGC ACCTGC(4/8) 623 ACCTGC BsrI 455 ACTGG ACTGG(1/-1) 909 CCAGT DdeI 656 CTGAG C^T[ACGT]AG 1101 CTCAG DraII 102 GGGTCCC [GA]G^G[ACGT]CC[CT] 807 AGGCCCC EcoO109I 102 GGGTCCC [GA]G^G[ACGT]CC[CT] 807 AGGCCCC HincII 355 GTCAAC GT[CT]^[GA]AC 514 GTTGAC HindII 355 GTCAAC GT[CT]^[GA]AC 514 GTTGAC HinfI 450 GATTC G^A[ACGT]TC 1151 GAGTC MaeII 145 ACGT A^CGT 262 ACGT SapI 58 GAAGAGC GCTCTTC(1/4) 972 GCTCTTC SmaI 988 CCCGGG CCC^GGG 1163 CCCGGG TaqI 240 TCGA T^CGA 1149 TCGA Tsp45I 586 GTGAC ^GT[GC]AC 1005 GTCAC TthHB8I 240 TCGA T^CGA 1149 TCGA XmaCI 988 CCCGGG C^CCGGG 1163 CCCGGG XmaI 988 CCCGGG C^CCGGG 1163 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaI AvaII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI BsmI BsoBI Bsp1286I BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI TspRI