################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BamHI BbeI BbrPI BcgI BciVI BclI BcnI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FseI HaeII HapII HincII HindII HpaI HpaII KasI KpnI KspI MamI MflI MluI MroI MslI MspI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SpeI Sse8387I StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcyI 1105 GGCGTC G[GA]^CG[CT]C AflII 1273 CTTAAG C^TTAAG AflIII 1693 ACATGT A^C[GA][CT]GT AlwI 260 GGATC GGATC(4/5) AlwNI 1818 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 89 GGACC G^G[AT]CC AviII 1620 TGCGCA TGC^GCA BalI 1562 TGGCCA TGG^CCA BanI 2022 GGTGCC G^G[CT][GA]CC BbsI 680 GTCTTC GAAGAC(2/6) BbvCI 649 GCTGAGG CCTCAGC(-5/-2) BfrI 1273 CTTAAG C^TTAAG BpuAI 680 GTCTTC GAAGAC(2/6) BsaAI 1316 TACGTA [CT]AC^GT[GA] BsaHI 1105 GGCGTC G[GA]^CG[CT]C Bsp1407I 1920 TGTACA T^GTACA BspLU11I 1693 ACATGT A^CATGT BsrGI 1920 TGTACA T^GTACA BssSI 272 CTCGTG CACGAG(-5/-1) CfoI 1621 GCGC GCG^C Cfr13I 89 GGACC G^G[ACGT]CC DraIII 610 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1764 CCGTGG C^C[GA][CT]GG EcoT22I 1430 ATGCAT ATGCA^T FspI 1620 TGCGCA TGC^GCA HgaI 1106 GCGTC GACGC(5/10) HhaI 1621 GCGC GCG^C Hin1I 1105 GGCGTC G[GA]^CG[CT]C HinP1I 1621 GCGC G^CGC HindIII 1301 AAGCTT A^AGCTT HphI 2005 GGTGA GGTGA(8/7) MluNI 1562 TGGCCA TGG^CCA MscI 1562 TGGCCA TGG^CCA NdeI 1550 CATATG CA^TATG NsiI 1430 ATGCAT ATGCA^T PvuII 347 CAGCTG CAG^CTG Sau96I 89 GGACC G^G[ACGT]CC ScaI 1203 AGTACT AGT^ACT SnaBI 1316 TACGTA TAC^GTA SspBI 1920 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### BfaI 2155 CTAG C^TAG 2287 CTAG BglI 723 GCCTGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1459 GCCTATGCGGC BpmI 1199 CTCCAG CTGGAG(16/14) 364 CTGGAG BsaI 47 GAGACC GGTCTC(1/5) 2083 GGTCTC BsaJI 326 CCCTGG C^C[ACGT][ACGT]GG 1764 CCGTGG BsiYI 1607 CCAAATCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1870 CCCGTGCAAGG BslI 1607 CCAAATCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1870 CCCGTGCAAGG BsrDI 1983 CATTGC GCAATG(2/0) 1237 GCAATG BstBI 284 TTCGAA TT^CGAA 422 TTCGAA DpnII 261 GATC ^GATC 2037 GATC DraI 27 TTTAAA TTT^AAA 708 TTTAAA EaeI 1562 TGGCCA [CT]^GGCC[GA] 2139 CGGCCA MaeI 2155 CTAG C^TAG 2287 CTAG MaeII 319 ACGT A^CGT 1317 ACGT MboI 261 GATC ^GATC 2037 GATC MfeI 266 CAATTG C^AATTG 1544 CAATTG MspA1I 347 CAGCTG C[AC]G^C[GT]G 1818 CAGCGG MunI 266 CAATTG C^AATTG 1544 CAATTG NdeII 261 GATC ^GATC 2037 GATC NspV 284 TTCGAA TT^CGAA 422 TTCGAA PleI 1380 GACTC GAGTC(4/5) 562 GAGTC PstI 1852 CTGCAG CTGCA^G 1977 CTGCAG SacI 230 GAGCTC GAGCT^C 2380 GAGCTC Sau3AI 261 GATC ^GATC 2037 GATC SfuI 284 TTCGAA TT^CGAA 422 TTCGAA SmlI 1273 CTTAAG C^T[CT][GA]AG 1955 CTCAAG SphI 1278 GCATGC GCATG^C 2250 GCATGC SspI 1053 AATATT AAT^ATT 1595 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI BanII BbvI BmyI BsgI BsiHKAI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI EarI EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeIII MboII MnlI MseI MvaI MwoI NlaIII NlaIV NspI RsaI SapI ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I