Restriction map for pFN21AE6756
 (Circular) (Six-base) MAPSORT of: pFN21AE6756.seq  Check: 1753  from: 1  to: 7141



pFN21AE6756  7141 bp

 With 182 enzymes: SgfI * 

                        March 18, 2015 14:54  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4951     278 
   Size:         53      83     186     936    3415    2468

  Fragments arranged by size: 

                3415    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    4484    1937 
   Size:       2547    4594
 
Acc65I G'GTAC_C

Cuts at:   4462    4462 
   Size:       7141
 
AclI AA'CG_TT

Cuts at:   1563    5268    5641    1563 
   Size:       3705     373    3063

  Fragments arranged by size: 

                3705    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2712    3324    3365    4203    5204    6588    6856    1632 
   Size:       1080     612      41     838    1001    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1080    1001     838     612     268      41
            
 
AflII C'TTAA_G

Cuts at:    820    1017    3278     820 
   Size:        197    2261    4683

  Fragments arranged by size: 

                4683    2261     197
 
AflIII A'CryG_T

Cuts at:   1203    3698    6040    7017    1203 
   Size:       2495    2342     977    1327

  Fragments arranged by size: 

                2495    2342    1327     977
 
AgeI A'CCGG_T

Cuts at:   5972    5972 
   Size:       7141
 
AhdI GACnn_n'nnGTC

Cuts at:   5870    5870 
   Size:       7141
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       7141
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3651    1184 
   Size:       2467    4674
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2770    2802    2770 
   Size:         32    7109
 
AlwNI CAG_nnn'CTG

Cuts at:   3828    4884    6456    3828 
   Size:       1056    1572    4513

  Fragments arranged by size: 

                4513    1572    1056
 
ApaLI G'TGCA_C

Cuts at:   5198    6354    5198 
   Size:       1156    5985
 
ApoI r'AATT_y

Cuts at:   1493    2207    2709    2868    2955    3081    3709    4194    4450 
   Size:        714     502     159      87     126     628     485     256

Cuts at:   4450    4676    4828    6937    1493 
   Size:        226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     714     628     502     485     256     226
                 159     152     126      87
 
AseI AT'TA_AT

Cuts at:    160    1032    5695     160 
   Size:        872    4663    1606

  Fragments arranged by size: 

                4663    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7141
 
AvaI C'yCGr_G

Cuts at:   1870    1943    4466    1870 
   Size:         73    2523    4545

  Fragments arranged by size: 

                4545    2523      73
 
BamHI G'GATC_C

Cuts at:   4471    4838    4471 
   Size:        367    6774
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    4027    4462    5917     618 
   Size:        325     217     627    2240     435    1455    1842

  Fragments arranged by size: 

                2240    1842    1455     627     435     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2239    4177    4389    4460    1391 
   Size:        848    1938     212      71    4072

  Fragments arranged by size: 

                4072    1938     848     212      71
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2678     928 
   Size:        971     779    5391

  Fragments arranged by size: 

                5391     971     779
 
BbvCI CC'TCA_GC

Cuts at:   2655    2655 
   Size:       7141
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    5332    5366    1903 
   Size:         34    3395      34    3678

  Fragments arranged by size: 

                3678    3395      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   5033    6243    5033 
   Size:       1210    5931
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       7141
 
BfrBI ATG'CAT

Cuts at:   3437    3437 
   Size:       7141
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2734    3470    5752 
   Size:        107     122      71     631     605    1062     736    2282

Cuts at:   5752     136 
   Size:       1525

  Fragments arranged by size: 

                2282    1525    1062     736     631     605     122     107
                  71
 
BglII A'GATC_T

Cuts at:   7136    7136 
   Size:       7141
 
BlpI GC'TnA_GC

Cuts at:   4558    4558 
   Size:       7141
 
Bme1580I G_kGCm'C

Cuts at:   1790    5202    6358    1790 
   Size:       3412    1156    2573

  Fragments arranged by size: 

                3412    2573    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       7141
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4872    5830     442 
   Size:        416      53      83     399      24    3455     958    1753

  Fragments arranged by size: 

                3455    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       7141
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3219    3599    3219 
   Size:        380    6761
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2390    3189    5801    1239 
   Size:       1151     799    2612    2579

  Fragments arranged by size: 

                2612    2579    1151     799
 
Bpu10I CC'TnA_GC

Cuts at:   2655    2655 
   Size:       7141
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3945    5266    6131    6429    3945 
   Size:       1321     865     298    4657

  Fragments arranged by size: 

                4657    1321     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2046    4094    5804     882 
   Size:        617     547    2048    1710    2219

  Fragments arranged by size: 

                2219    2048    1710     617     547
 
BsaAI yAC'GTr

Cuts at:    493    1204    3323    4932     493 
   Size:        711    2119    1609    2702

  Fragments arranged by size: 

                2702    2119    1609     711
 
BsaBI GATnn'nnATC

Cuts at:   7135    7135 
   Size:       7141
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3111    4948    5330    5965 
   Size:         53      83     186     936    1578    1837     382     635

Cuts at:   5965     275 
   Size:       1451

  Fragments arranged by size: 

                1837    1578    1451     936     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   5574    5972    6246    6393    6804    5574 
   Size:        398     274     147     411    5911

  Fragments arranged by size: 

                5911     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3918    3948    1178 
   Size:         30     165      30    2515      30    4371

  Fragments arranged by size: 

                4371    2515     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       7141
 
BseYI C'CCAG_C

Cuts at:   6344    6840    6344 
   Size:        496    6645
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2349    2454    2597    4331    4457    1163 
   Size:        573     613     105     143    1734     126    3847

  Fragments arranged by size: 

                3847    1734     613     573     143     126     105
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4591    5352    6380    1540 
   Size:        275     186    2590     761    1028    2301

  Fragments arranged by size: 

                2590    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2239    2485    3975    4132    4389    5202    5287    6358    2239 
   Size:        246    1490     157     257     813      85    1071    3022

  Fragments arranged by size: 

                3022    1490    1071     813     257     246     157      85
            
 
BsmI GAATG_Cn'

Cuts at:   2469    3437    4429    4652    4745    2469 
   Size:        968     992     223      93    4865

  Fragments arranged by size: 

                4865     992     968     223      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2239    2485    3975    4132    4177    4389    4460 
   Size:        399     449     246    1490     157      45     212      71

Cuts at:   4460    5202    5287    6358    1391 
   Size:        742      85    1071    2174

  Fragments arranged by size: 

                2174    1490    1071     742     449     399     246     212
                 157      85      71      45
 
BspEI T'CCGG_A

Cuts at:   6804    6804 
   Size:       7141
 
BspHI T'CATG_A

Cuts at:   5030    6760    5030 
   Size:       1730    5411
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    4429    4496     844 
   Size:       3585      67    3489

  Fragments arranged by size: 

                3585    3489      67
 
BsrBI CCG'CTC

Cuts at:   1394    1823    5028    1394 
   Size:        429    3205    3507

  Fragments arranged by size: 

                3507    3205     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3249    3987    5636    5810      65 
   Size:       1183    2001     738    1649     174    1396

  Fragments arranged by size: 

                2001    1649    1396    1183     738     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5785    5972    1700 
   Size:        110    3975     187    2869

  Fragments arranged by size: 

                3975    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    3925      96 
   Size:       3829    3312
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7141
 
BssSI C'ACGA_G

Cuts at:   2277    5201    6213    2277 
   Size:       2924    1012    3205

  Fragments arranged by size: 

                3205    2924    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       7141
 
BstBI TT'CG_AA

Cuts at:   2290    2428    5945    2290 
   Size:        138    3517    3486

  Fragments arranged by size: 

                3517    3486     138
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       7141
 
BstYI r'GATC_y

Cuts at:   1972    4471    4838    5223    5240    6692    6778    6790    7136 
   Size:       2499     367     385      17    1452      86      12     346

Cuts at:   7136    1972 
   Size:       1977

  Fragments arranged by size: 

                2499    1977    1452     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    3769     513 
   Size:        552     939    1765    3885

  Fragments arranged by size: 

                3885    1765     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    3985     487 
   Size:        683      56    2759    3643

  Fragments arranged by size: 

                3643    2759     683      56
 
BtsI GCAGTG_nn'

Cuts at:   4657    5451    5471    4657 
   Size:        794      20    6327

  Fragments arranged by size: 

                6327     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       7141
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    7106
 
DraI TTT'AAA

Cuts at:   2034    2715    4445    2034 
   Size:        681    1730    4730

  Fragments arranged by size: 

                4730    1730     681
 
DraIII CAC_nnn'GTG

Cuts at:   2620    2620 
   Size:       7141
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    6148     809 
   Size:        507    4832    1802

  Fragments arranged by size: 

                4832    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3567    4144    4588 
   Size:         54    1425      50     275      18    1737     577     444

Cuts at:   4588    5477    7106       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1737    1629    1425     889     577     444     275      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4588    1537 
   Size:        275    2776    4090

  Fragments arranged by size: 

                4090    2776     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2139    2618    3366    3980    4182    5071    1353 
   Size:        786     479     748     614     202     889    3423

  Fragments arranged by size: 

                3423     889     786     748     614     479     202
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2486    5728    6102    6248     387 
   Size:       1064     138     897    3242     374     146    1280

  Fragments arranged by size: 

                3242    1280    1064     897     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2237    4387    2237 
   Size:       2150    4991
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2390    2712    3189    3324    3365    4203    5204 
   Size:        393     758     322     477     135      41     838    1001

Cuts at:   5204    5801    6588    6856    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524    1001     838     787     758     597     477     393
                 322     268     135      41
 
EcoRI G'AATT_C

Cuts at:   4450    4828    4450 
   Size:        378    6763
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2656    3395    2656 
   Size:        739    6402
 
FspI TGC'GCA

Cuts at:   3627    5647    3627 
   Size:       2020    5121
 
HaeII r_GCGC'y

Cuts at:   1678    6288    1678 
   Size:       4610    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2309    2341    2371    2403    5782 
   Size:         32     135      32     737      32      30      32    3379

Cuts at:   5782    5814    5856    5888    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                3379    2626     737     135      42      32      32      32
                  32      32      32      30
 
HincII GTy'rAC

Cuts at:    669    1657    1938    4485    4731     669 
   Size:        988     281    2547     246    3079

  Fragments arranged by size: 

                3079    2547     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3306     748 
   Size:       2558    4583
 
HpaI GTT'AAC

Cuts at:   4731    4731 
   Size:       7141
 
KpnI G_GTAC'C

Cuts at:   4466    4466 
   Size:       7141
 
MfeI C'AATT_G

Cuts at:   2271    3549    4740    2271 
   Size:       1278    1191    4672

  Fragments arranged by size: 

                4672    1278    1191
 
MluI A'CGCG_T

Cuts at:   7017    7017 
   Size:       7141
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2369    3624    4096    4185    6255    6439    1305 
   Size:       1064    1255     472      89    2070     184    2007

  Fragments arranged by size: 

                2070    2007    1255    1064     472     184      89
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    3569      10 
   Size:         54    1425     343    1737    3582

  Fragments arranged by size: 

                3582    1737    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    5099    5458    5617     518 
   Size:        648    3933     359     159    2042

  Fragments arranged by size: 

                3933    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2354    3825    4557    5234    6382    6627    1606 
   Size:        748    1471     732     677    1148     245    2120

  Fragments arranged by size: 

                2120    1471    1148     748     732     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    5650

  Fragments arranged by size: 

                5650     939     552
 
NdeI CA'TA_TG

Cuts at:    387    3556     387 
   Size:       3169    3972
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       7141
 
NotI GC'GGCC_GC

Cuts at:   4588    4588 
   Size:       7141
 
NsiI A_TGCA'T

Cuts at:   3439    3439 
   Size:       7141
 
NspI r_CATG'y

Cuts at:   1132    1229    2489    3287    3532    3702    4259    4290    4499 
   Size:         97    1260     798     245     170     557      31     209

Cuts at:   4499    6044    1132 
   Size:       1545    2229

  Fragments arranged by size: 

                2229    1545    1260     798     557     245     209     170
                  97      31
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       7141
 
PciI A'CATG_T

Cuts at:   3698    6040    3698 
   Size:       2342    4799
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       7141
 
PfoI T'CCnGG_A

Cuts at:   3101    3101 
   Size:       7141
 
PmeI GTTT'AAAC

Cuts at:   4445    4445 
   Size:       7141
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3515    3547    5210    5242    6723    6755    3515 
   Size:         32    1663      32    1481      32    3901

  Fragments arranged by size: 

                3901    1663    1481      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       7141
 
PsiI TTA'TAA

Cuts at:   2819    4711    2819 
   Size:       1892    5249
 
PstI C_TGCA'G

Cuts at:    830    3861    3986    4493     830 
   Size:       3031     125     507    3478

  Fragments arranged by size: 

                3478    3031     507     125
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       7141
 
PvuII CAG'CTG

Cuts at:   2354    2354 
   Size:       7141
 
SacI G_AGCT'C

Cuts at:   2239    4389    2239 
   Size:       2150    4991
 
SalI G'TCGA_C

Cuts at:   1936    4483    1936 
   Size:       2547    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2618    3980    4182    2618 
   Size:       1362     202    5577

  Fragments arranged by size: 

                5577    1362     202
 
SbfI CC_TGCA'GG

Cuts at:   4493    4493 
   Size:       7141
 
ScaI AGT'ACT

Cuts at:   3210    5389    7101    3210 
   Size:       2179    1712    3250

  Fragments arranged by size: 

                3250    2179    1712
 
SfcI C'TryA_G

Cuts at:    826    1045    3327    3857    3982    4489    5624    6305    6496 
   Size:        219    2282     530     125     507    1135     681     191

Cuts at:   6496     826 
   Size:       1471

  Fragments arranged by size: 

                2282    1471    1135     681     530     507     219     191
                 125
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7141
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7141
 
SmaI CCC'GGG

Cuts at:   1872    4468    1872 
   Size:       2596    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3278    3960    5245    6146    6408     820 
   Size:        197     926    1335     682    1285     901     262    1553

  Fragments arranged by size: 

                1553    1335    1285     926     901     682     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    3323    4932     493 
   Size:       2830    1609    2702

  Fragments arranged by size: 

                2830    2702    1609
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       7141
 
SphI G_CATG'C

Cuts at:   1132    3287    4259    4499    1132 
   Size:       2155     972     240    3774

  Fragments arranged by size: 

                3774    2155     972     240
 
SspI AAT'ATT

Cuts at:      5      52    3060    3602    5065       5 
   Size:         47    3008     542    1463    2081

  Fragments arranged by size: 

                3008    2081    1463     542      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       7141
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    4580     513 
   Size:        552     939    2576    3074

  Fragments arranged by size: 

                3074    2576     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2681    2885    5168    5185    5338    5523    5968 
   Size:       1255    1101     204    2283      17     153     185     445

Cuts at:   5968     325 
   Size:       1498

  Fragments arranged by size: 

                2283    1498    1255    1101     445     204     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3208    3925    5387 
   Size:        274      80      33      51    1443    1231     717    1462

Cuts at:   5387    7099      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1462    1443    1231     717     274     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   7101    7133    7101 
   Size:         32    7109
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       7141
 
XbaI T'CTAG_A

Cuts at:   4477    4477 
   Size:       7141
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7141
 
XmaI C'CCGG_G

Cuts at:   1870    4466    1870 
   Size:       2596    4545
 
XmnI GAAnn'nnTTC

Cuts at:   5270    5270 
   Size:       7141
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4949     276 
   Size:         53      83     186     936    3415    2468

  Fragments arranged by size: 

                3415    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PshAI     PsiI     PstI     PvuI    PvuII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BsiWI
    BsmBI    BstXI  BstZ17I   Bsu36I    EcoNI EcoO109I    EcoRV     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     PacI     PmlI
    PpuMI   PspOMI    PspXI     PsrI    RsrII    SacII    SanDI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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