(Circular) (Six-base) MAPSORT of: pFN21AE6756.seq Check: 1753 from: 1 to: 7141 pFN21AE6756 7141 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4951 278 Size: 53 83 186 936 3415 2468 Fragments arranged by size: 3415 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4484 1937 Size: 2547 4594 Acc65I G'GTAC_C Cuts at: 4462 4462 Size: 7141 AclI AA'CG_TT Cuts at: 1563 5268 5641 1563 Size: 3705 373 3063 Fragments arranged by size: 3705 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2712 3324 3365 4203 5204 6588 6856 1632 Size: 1080 612 41 838 1001 1384 268 1917 Fragments arranged by size: 1917 1384 1080 1001 838 612 268 41 AflII C'TTAA_G Cuts at: 820 1017 3278 820 Size: 197 2261 4683 Fragments arranged by size: 4683 2261 197 AflIII A'CryG_T Cuts at: 1203 3698 6040 7017 1203 Size: 2495 2342 977 1327 Fragments arranged by size: 2495 2342 1327 977 AgeI A'CCGG_T Cuts at: 5972 5972 Size: 7141 AhdI GACnn_n'nnGTC Cuts at: 5870 5870 Size: 7141 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7141 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3651 1184 Size: 2467 4674 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2770 2802 2770 Size: 32 7109 AlwNI CAG_nnn'CTG Cuts at: 3828 4884 6456 3828 Size: 1056 1572 4513 Fragments arranged by size: 4513 1572 1056 ApaLI G'TGCA_C Cuts at: 5198 6354 5198 Size: 1156 5985 ApoI r'AATT_y Cuts at: 1493 2207 2709 2868 2955 3081 3709 4194 4450 Size: 714 502 159 87 126 628 485 256 Cuts at: 4450 4676 4828 6937 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 714 628 502 485 256 226 159 152 126 87 AseI AT'TA_AT Cuts at: 160 1032 5695 160 Size: 872 4663 1606 Fragments arranged by size: 4663 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7141 AvaI C'yCGr_G Cuts at: 1870 1943 4466 1870 Size: 73 2523 4545 Fragments arranged by size: 4545 2523 73 BamHI G'GATC_C Cuts at: 4471 4838 4471 Size: 367 6774 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4027 4462 5917 618 Size: 325 217 627 2240 435 1455 1842 Fragments arranged by size: 2240 1842 1455 627 435 325 217 BanII G_rGCy'C Cuts at: 1391 2239 4177 4389 4460 1391 Size: 848 1938 212 71 4072 Fragments arranged by size: 4072 1938 848 212 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2678 928 Size: 971 779 5391 Fragments arranged by size: 5391 971 779 BbvCI CC'TCA_GC Cuts at: 2655 2655 Size: 7141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5332 5366 1903 Size: 34 3395 34 3678 Fragments arranged by size: 3678 3395 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5033 6243 5033 Size: 1210 5931 BclI T'GATC_A Cuts at: 1548 1548 Size: 7141 BfrBI ATG'CAT Cuts at: 3437 3437 Size: 7141 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2734 3470 5752 Size: 107 122 71 631 605 1062 736 2282 Cuts at: 5752 136 Size: 1525 Fragments arranged by size: 2282 1525 1062 736 631 605 122 107 71 BglII A'GATC_T Cuts at: 7136 7136 Size: 7141 BlpI GC'TnA_GC Cuts at: 4558 4558 Size: 7141 Bme1580I G_kGCm'C Cuts at: 1790 5202 6358 1790 Size: 3412 1156 2573 Fragments arranged by size: 3412 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7141 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4872 5830 442 Size: 416 53 83 399 24 3455 958 1753 Fragments arranged by size: 3455 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7141 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3219 3599 3219 Size: 380 6761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2390 3189 5801 1239 Size: 1151 799 2612 2579 Fragments arranged by size: 2612 2579 1151 799 Bpu10I CC'TnA_GC Cuts at: 2655 2655 Size: 7141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3945 5266 6131 6429 3945 Size: 1321 865 298 4657 Fragments arranged by size: 4657 1321 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2046 4094 5804 882 Size: 617 547 2048 1710 2219 Fragments arranged by size: 2219 2048 1710 617 547 BsaAI yAC'GTr Cuts at: 493 1204 3323 4932 493 Size: 711 2119 1609 2702 Fragments arranged by size: 2702 2119 1609 711 BsaBI GATnn'nnATC Cuts at: 7135 7135 Size: 7141 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3111 4948 5330 5965 Size: 53 83 186 936 1578 1837 382 635 Cuts at: 5965 275 Size: 1451 Fragments arranged by size: 1837 1578 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5574 5972 6246 6393 6804 5574 Size: 398 274 147 411 5911 Fragments arranged by size: 5911 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3918 3948 1178 Size: 30 165 30 2515 30 4371 Fragments arranged by size: 4371 2515 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7141 BseYI C'CCAG_C Cuts at: 6344 6840 6344 Size: 496 6645 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2349 2454 2597 4331 4457 1163 Size: 573 613 105 143 1734 126 3847 Fragments arranged by size: 3847 1734 613 573 143 126 105 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4591 5352 6380 1540 Size: 275 186 2590 761 1028 2301 Fragments arranged by size: 2590 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2239 2485 3975 4132 4389 5202 5287 6358 2239 Size: 246 1490 157 257 813 85 1071 3022 Fragments arranged by size: 3022 1490 1071 813 257 246 157 85 BsmI GAATG_Cn' Cuts at: 2469 3437 4429 4652 4745 2469 Size: 968 992 223 93 4865 Fragments arranged by size: 4865 992 968 223 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2239 2485 3975 4132 4177 4389 4460 Size: 399 449 246 1490 157 45 212 71 Cuts at: 4460 5202 5287 6358 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1490 1071 742 449 399 246 212 157 85 71 45 BspEI T'CCGG_A Cuts at: 6804 6804 Size: 7141 BspHI T'CATG_A Cuts at: 5030 6760 5030 Size: 1730 5411 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4429 4496 844 Size: 3585 67 3489 Fragments arranged by size: 3585 3489 67 BsrBI CCG'CTC Cuts at: 1394 1823 5028 1394 Size: 429 3205 3507 Fragments arranged by size: 3507 3205 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3249 3987 5636 5810 65 Size: 1183 2001 738 1649 174 1396 Fragments arranged by size: 2001 1649 1396 1183 738 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5785 5972 1700 Size: 110 3975 187 2869 Fragments arranged by size: 3975 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3925 96 Size: 3829 3312 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7141 BssSI C'ACGA_G Cuts at: 2277 5201 6213 2277 Size: 2924 1012 3205 Fragments arranged by size: 3205 2924 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7141 BstBI TT'CG_AA Cuts at: 2290 2428 5945 2290 Size: 138 3517 3486 Fragments arranged by size: 3517 3486 138 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7141 BstYI r'GATC_y Cuts at: 1972 4471 4838 5223 5240 6692 6778 6790 7136 Size: 2499 367 385 17 1452 86 12 346 Cuts at: 7136 1972 Size: 1977 Fragments arranged by size: 2499 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3769 513 Size: 552 939 1765 3885 Fragments arranged by size: 3885 1765 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3985 487 Size: 683 56 2759 3643 Fragments arranged by size: 3643 2759 683 56 BtsI GCAGTG_nn' Cuts at: 4657 5451 5471 4657 Size: 794 20 6327 Fragments arranged by size: 6327 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7141 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7106 DraI TTT'AAA Cuts at: 2034 2715 4445 2034 Size: 681 1730 4730 Fragments arranged by size: 4730 1730 681 DraIII CAC_nnn'GTG Cuts at: 2620 2620 Size: 7141 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6148 809 Size: 507 4832 1802 Fragments arranged by size: 4832 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3567 4144 4588 Size: 54 1425 50 275 18 1737 577 444 Cuts at: 4588 5477 7106 8 Size: 889 1629 43 Fragments arranged by size: 1737 1629 1425 889 577 444 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4588 1537 Size: 275 2776 4090 Fragments arranged by size: 4090 2776 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2139 2618 3366 3980 4182 5071 1353 Size: 786 479 748 614 202 889 3423 Fragments arranged by size: 3423 889 786 748 614 479 202 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2486 5728 6102 6248 387 Size: 1064 138 897 3242 374 146 1280 Fragments arranged by size: 3242 1280 1064 897 374 146 138 EcoICRI GAG'CTC Cuts at: 2237 4387 2237 Size: 2150 4991 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2390 2712 3189 3324 3365 4203 5204 Size: 393 758 322 477 135 41 838 1001 Cuts at: 5204 5801 6588 6856 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1001 838 787 758 597 477 393 322 268 135 41 EcoRI G'AATT_C Cuts at: 4450 4828 4450 Size: 378 6763 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2656 3395 2656 Size: 739 6402 FspI TGC'GCA Cuts at: 3627 5647 3627 Size: 2020 5121 HaeII r_GCGC'y Cuts at: 1678 6288 1678 Size: 4610 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2309 2341 2371 2403 5782 Size: 32 135 32 737 32 30 32 3379 Cuts at: 5782 5814 5856 5888 1373 Size: 32 42 32 2626 Fragments arranged by size: 3379 2626 737 135 42 32 32 32 32 32 32 30 HincII GTy'rAC Cuts at: 669 1657 1938 4485 4731 669 Size: 988 281 2547 246 3079 Fragments arranged by size: 3079 2547 988 281 246 HindIII A'AGCT_T Cuts at: 748 3306 748 Size: 2558 4583 HpaI GTT'AAC Cuts at: 4731 4731 Size: 7141 KpnI G_GTAC'C Cuts at: 4466 4466 Size: 7141 MfeI C'AATT_G Cuts at: 2271 3549 4740 2271 Size: 1278 1191 4672 Fragments arranged by size: 4672 1278 1191 MluI A'CGCG_T Cuts at: 7017 7017 Size: 7141 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2369 3624 4096 4185 6255 6439 1305 Size: 1064 1255 472 89 2070 184 2007 Fragments arranged by size: 2070 2007 1255 1064 472 184 89 MscI TGG'CCA Cuts at: 10 64 1489 1832 3569 10 Size: 54 1425 343 1737 3582 Fragments arranged by size: 3582 1737 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5099 5458 5617 518 Size: 648 3933 359 159 2042 Fragments arranged by size: 3933 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2354 3825 4557 5234 6382 6627 1606 Size: 748 1471 732 677 1148 245 2120 Fragments arranged by size: 2120 1471 1148 748 732 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5650 Fragments arranged by size: 5650 939 552 NdeI CA'TA_TG Cuts at: 387 3556 387 Size: 3169 3972 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7141 NotI GC'GGCC_GC Cuts at: 4588 4588 Size: 7141 NsiI A_TGCA'T Cuts at: 3439 3439 Size: 7141 NspI r_CATG'y Cuts at: 1132 1229 2489 3287 3532 3702 4259 4290 4499 Size: 97 1260 798 245 170 557 31 209 Cuts at: 4499 6044 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1260 798 557 245 209 170 97 31 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7141 PciI A'CATG_T Cuts at: 3698 6040 3698 Size: 2342 4799 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7141 PfoI T'CCnGG_A Cuts at: 3101 3101 Size: 7141 PmeI GTTT'AAAC Cuts at: 4445 4445 Size: 7141 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3515 3547 5210 5242 6723 6755 3515 Size: 32 1663 32 1481 32 3901 Fragments arranged by size: 3901 1663 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7141 PsiI TTA'TAA Cuts at: 2819 4711 2819 Size: 1892 5249 PstI C_TGCA'G Cuts at: 830 3861 3986 4493 830 Size: 3031 125 507 3478 Fragments arranged by size: 3478 3031 507 125 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7141 PvuII CAG'CTG Cuts at: 2354 2354 Size: 7141 SacI G_AGCT'C Cuts at: 2239 4389 2239 Size: 2150 4991 SalI G'TCGA_C Cuts at: 1936 4483 1936 Size: 2547 4594 SapI GCTCTTCn'nnn_ Cuts at: 2618 3980 4182 2618 Size: 1362 202 5577 Fragments arranged by size: 5577 1362 202 SbfI CC_TGCA'GG Cuts at: 4493 4493 Size: 7141 ScaI AGT'ACT Cuts at: 3210 5389 7101 3210 Size: 2179 1712 3250 Fragments arranged by size: 3250 2179 1712 SfcI C'TryA_G Cuts at: 826 1045 3327 3857 3982 4489 5624 6305 6496 Size: 219 2282 530 125 507 1135 681 191 Cuts at: 6496 826 Size: 1471 Fragments arranged by size: 2282 1471 1135 681 530 507 219 191 125 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7141 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7141 SmaI CCC'GGG Cuts at: 1872 4468 1872 Size: 2596 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3278 3960 5245 6146 6408 820 Size: 197 926 1335 682 1285 901 262 1553 Fragments arranged by size: 1553 1335 1285 926 901 682 262 197 SnaBI TAC'GTA Cuts at: 493 3323 4932 493 Size: 2830 1609 2702 Fragments arranged by size: 2830 2702 1609 SpeI A'CTAG_T Cuts at: 152 152 Size: 7141 SphI G_CATG'C Cuts at: 1132 3287 4259 4499 1132 Size: 2155 972 240 3774 Fragments arranged by size: 3774 2155 972 240 SspI AAT'ATT Cuts at: 5 52 3060 3602 5065 5 Size: 47 3008 542 1463 2081 Fragments arranged by size: 3008 2081 1463 542 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7141 StyI C'CwwG_G Cuts at: 513 1065 2004 4580 513 Size: 552 939 2576 3074 Fragments arranged by size: 3074 2576 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2681 2885 5168 5185 5338 5523 5968 Size: 1255 1101 204 2283 17 153 185 445 Cuts at: 5968 325 Size: 1498 Fragments arranged by size: 2283 1498 1255 1101 445 204 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3208 3925 5387 Size: 274 80 33 51 1443 1231 717 1462 Cuts at: 5387 7099 96 Size: 1712 138 Fragments arranged by size: 1712 1462 1443 1231 717 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7101 7133 7101 Size: 32 7109 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7141 XbaI T'CTAG_A Cuts at: 4477 4477 Size: 7141 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7141 XmaI C'CCGG_G Cuts at: 1870 4466 1870 Size: 2596 4545 XmnI GAAnn'nnTTC Cuts at: 5270 5270 Size: 7141 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4949 276 Size: 53 83 186 936 3415 2468 Fragments arranged by size: 3415 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI