Restriction map for
pFN21AE5262
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BalI BamHI BbrPI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FspI HincII
HindII HindIII HinfI HpaI HphI
KpnI Ksp632I KspI MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MunI NciI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
AccII 520 CGCG CG^CG
AcsI 463 AAATTC [GA]^AATT[CT]
AfaI 474 GTAC GT^AC
AflIII 413 ACATGT A^C[GA][CT]GT
AlwNI 429 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 463 AAATTC [GA]^AATT[CT]
Asp700I 459 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 304 GAGCTC G[AT]GC[AT]^C
AspI 529 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 73 GGCGCC G^G[CT][GA]CC
BanII 304 GAGCTC G[GA]GC[CT]^C
BbeI 73 GGCGCC GGCGC^C
BbsI 322 GTCTTC GAAGAC(2/6)
BfaI 215 CTAG C^TAG
BmyI 304 GAGCTC G[AGT]GC[ACT]^C
BpmI 78 CTCCAG CTGGAG(16/14)
BpuAI 322 GTCTTC GAAGAC(2/6)
BsaI 196 GAGACC GGTCTC(1/5)
BseRI 232 GAGGAG GAGGAG(10/8)
BsiEI 156 CGGCCG CG[GA][CT]^CG
BsiHKAI 304 GAGCTC G[AT]GC[AT]^C
BsmAI 196 GAGAC GTCTC(1/5)
Bsp1286I 304 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 413 ACATGT A^CATGT
BsrBI 237 GAGCGG CCGCTC(-3/-3)
BsrI 434 ACTGG ACTGG(1/-1)
BstUI 520 CGCG CG^CG
BstYI 398 AGATCC [GA]^GATC[CT]
DraII 346 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 262 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 313 GACGTGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 156 CGGCCG [CT]^GGCC[GA]
EagI 156 CGGCCG C^GGCCG
EclXI 156 CGGCCG C^GGCCG
Eco52I 156 CGGCCG C^GGCCG
EcoNI 258 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 346 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 68 CCATGG C^C[AT][AT]GG
FseI 153 GGCCGGCC GGCCGG^CC
HaeII 73 GGCGCC [GA]GCGC^[CT]
HgaI 253 GACGC GACGC(5/10)
KasI 73 GGCGCC G^GCGCC
MaeI 215 CTAG C^TAG
MaeII 314 ACGT A^CGT
MflI 398 AGATCC [GA]^GATC[CT]
MslI 309 CATGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 388 CAGCTG C[AC]G^C[GT]G
MvnI 520 CGCG CG^CG
NaeI 154 GCCGGC GCC^GGC
NarI 73 GGCGCC GG^CGCC
NcoI 68 CCATGG C^CATGG
NgoMIV 154 GCCGGC G^CCGGC
PflFI 529 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 346 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 388 CAGCTG CAG^CTG
RsaI 474 GTAC GT^AC
SacI 304 GAGCTC GAGCT^C
SfaNI 338 GCATC GCATC(5/9)
SfoI 73 GGCGCC GGC^GCC
StyI 68 CCATGG C^C[AT][AT]GG
TaqI 203 TCGA T^CGA
TspRI 248 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 529 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 203 TCGA T^CGA
XcmI 34 CCAGAAGGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 398 AGATCC [GA]^GATC[CT]
XmnI 459 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 519 CCGC CCGC(-3/-1)
239 GCGG
AcyI 73 GGCGCC G[GA]^CG[CT]C
253 GACGCC
AlwI 399 GATCC GGATC(4/5)
393 GGATC
BbvCI 16 CCTCAGC CCTCAGC(-5/-2)
112 CCTCAGC
BbvI 108 GCAGC GCAGC(8/12)
225 GCTGC
BsaHI 73 GGCGCC G[GA]^CG[CT]C
253 GACGCC
BsgI 259 CTGCAC GTGCAG(16/14)
327 CTGCAC
BsmFI 64 GGGAC GGGAC(10/14)
346 GGGAC
BsrFI 154 GCCGGC [GA]^CCGG[CT]
158 GCCGGT
Cac8I 154 GCCGGC GC[ACGT]^[ACGT]GC
296 GCGAGC
CfoI 74 GCGC GCG^C
372 GCGC
Cfr10I 154 GCCGGC [GA]^CCGG[CT]
158 GCCGGT
Fnu4HI 108 GCAGC GC^[ACGT]GC
225 GCTGC
HapII 155 CCGG C^CGG
159 CCGG
HhaI 74 GCGC GCG^C
372 GCGC
Hin1I 73 GGCGCC G[GA]^CG[CT]C
253 GACGCC
HinP1I 74 GCGC G^CGC
372 GCGC
HpaII 155 CCGG C^CGG
159 CCGG
ItaI 108 GCAGC GC^[ACGT]GC
225 GCTGC
MspI 155 CCGG C^CGG
159 CCGG
NspI 318 GCATGT [GA]CATG^[CT]
413 ACATGT
PstI 106 CTGCAG CTGCA^G
226 CTGCAG
SfcI 106 CTGCAG C^T[GA][CT]AG
226 CTGCAG
TseI 108 GCAGC G^C[AT]GC
225 GCTGC
Tsp509I 9 AATT ^AATT
464 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI AvaII BsaJI BsiYI BslI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DsaI EcoRII FokI HaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI