(Linear) (Six-base) MAPSORT of: KIEE5262.seq Check: 1489 from: 1 to: 537 KIEE5262.orf With 182 enzymes: SgfI * January 29, 2014 11:53 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 436 537 Size: 436 101 AflIII A'CryG_T Cuts at: 0 413 537 Size: 413 124 AlwNI CAG_nnn'CTG Cuts at: 0 434 537 Size: 434 103 ApoI r'AATT_y Cuts at: 0 463 537 Size: 463 74 BanI G'GyrC_C Cuts at: 0 73 537 Size: 73 464 BanII G_rGCy'C Cuts at: 0 308 537 Size: 308 229 BbeI G_GCGC'C Cuts at: 0 77 537 Size: 77 460 BbsI GAAGACnn'nnnn_ Cuts at: 0 315 537 Size: 315 222 BbvCI CC'TCA_GC Cuts at: 0 17 113 537 Size: 17 96 424 Fragments arranged by size: 424 96 17 BmgBI CAC'GTC Cuts at: 0 315 537 Size: 315 222 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 63 537 Size: 63 474 Bpu10I CC'TnA_GC Cuts at: 0 17 113 302 537 Size: 17 96 189 235 Fragments arranged by size: 235 189 96 17 BsaI GGTCTCn'nnnn_ Cuts at: 0 190 537 Size: 190 347 BsaHI Gr'CG_yC Cuts at: 0 74 254 537 Size: 74 180 283 Fragments arranged by size: 283 180 74 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 247 537 Size: 247 290 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 312 537 Size: 244 68 225 Fragments arranged by size: 244 225 68 BsiEI CG_ry'CG Cuts at: 0 159 537 Size: 159 378 BsiHKAI G_wGCw'C Cuts at: 0 308 537 Size: 308 229 Bsp1286I G_dGCh'C Cuts at: 0 308 537 Size: 308 229 BsrBI CCG'CTC Cuts at: 0 239 537 Size: 239 298 BsrFI r'CCGG_y Cuts at: 0 154 158 537 Size: 154 4 379 Fragments arranged by size: 379 154 4 BstYI r'GATC_y Cuts at: 0 398 537 Size: 398 139 BtgI C'CryG_G Cuts at: 0 68 353 468 537 Size: 68 285 115 69 Fragments arranged by size: 285 115 69 68 DraIII CAC_nnn'GTG Cuts at: 0 267 537 Size: 267 270 DrdI GACnn_nn'nnGTC Cuts at: 0 319 537 Size: 319 218 EaeI y'GGCC_r Cuts at: 0 156 537 Size: 156 381 EagI C'GGCC_G Cuts at: 0 156 537 Size: 156 381 EcoICRI GAG'CTC Cuts at: 0 306 537 Size: 306 231 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 63 436 537 Size: 63 373 101 Fragments arranged by size: 373 101 63 EcoNI CCTnn'n_nnAGG Cuts at: 0 262 537 Size: 262 275 EcoO109I rG'GnC_Cy Cuts at: 0 347 537 Size: 347 190 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 162 537 Size: 162 375 FseI GG_CCGG'CC Cuts at: 0 158 537 Size: 158 379 HaeII r_GCGC'y Cuts at: 0 77 537 Size: 77 460 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 185 217 537 Size: 185 32 320 Fragments arranged by size: 320 185 32 KasI G'GCGC_C Cuts at: 0 73 537 Size: 73 464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 25 537 Size: 25 512 MslI CAynn'nnrTG Cuts at: 0 313 537 Size: 313 224 MspA1I CmG'CkG Cuts at: 0 390 537 Size: 390 147 NaeI GCC'GGC Cuts at: 0 156 537 Size: 156 381 NarI GG'CG_CC Cuts at: 0 74 537 Size: 74 463 NcoI C'CATG_G Cuts at: 0 68 537 Size: 68 469 NgoMIV G'CCGG_C Cuts at: 0 154 537 Size: 154 383 NspI r_CATG'y Cuts at: 0 322 417 537 Size: 322 95 120 Fragments arranged by size: 322 120 95 PasI CC'CwG_GG Cuts at: 0 61 537 Size: 61 476 PciI A'CATG_T Cuts at: 0 413 537 Size: 413 124 PfoI T'CCnGG_A Cuts at: 0 503 537 Size: 503 34 PpuMI rG'GwC_Cy Cuts at: 0 347 537 Size: 347 190 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 466 498 537 Size: 466 32 39 Fragments arranged by size: 466 39 32 PstI C_TGCA'G Cuts at: 0 110 230 537 Size: 110 120 307 Fragments arranged by size: 307 120 110 PvuII CAG'CTG Cuts at: 0 390 537 Size: 390 147 SacI G_AGCT'C Cuts at: 0 308 537 Size: 308 229 SanDI GG'GwC_CC Cuts at: 0 347 537 Size: 347 190 SfcI C'TryA_G Cuts at: 0 106 226 537 Size: 106 120 311 Fragments arranged by size: 311 120 106 SfoI GGC'GCC Cuts at: 0 75 537 Size: 75 462 StyI C'CwwG_G Cuts at: 0 68 537 Size: 68 469 Tth111I GACn'n_nGTC Cuts at: 0 532 537 Size: 532 5 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 41 537 Size: 41 496 XmnI GAAnn'nnTTC Cuts at: 0 463 537 Size: 463 74 Enzymes that do cut: AcuI AflIII AlwNI ApoI BanI BanII BbeI BbsI BbvCI BmgBI BpmI Bpu10I BsaI BsaHI BseRI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BstYI BtgI DraIII DrdI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PpuMI PsrI PstI PvuII SacI SanDI SfcI SfoI StyI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsaXI BseYI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI EarI EciI EcoRI EcoRV FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NdeI NheI NotI NruI NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI XbaI XhoI XmaI ZraI