################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MamI MluI MluNI MroI MscI MspA1I MvaI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 44 GAATTC [GA]^AATT[CT] AfaI 304 GTAC GT^AC AlwI 682 GATCC GGATC(4/5) ApoI 44 GAATTC [GA]^AATT[CT] BbsI 187 GAAGAC GAAGAC(2/6) BbvI 619 GCTGC GCAGC(8/12) BcnI 780 CCCGG CC^[GC]GG BfaI 643 CTAG C^TAG BglII 648 AGATCT A^GATCT BpuAI 187 GAAGAC GAAGAC(2/6) BsaJI 289 CCTTGG C^C[ACGT][ACGT]GG BseRI 652 CTCCTC GAGGAG(10/8) BsgI 337 CTGCAC GTGCAG(16/14) BsmAI 180 GAGAC GTCTC(1/5) BsrI 489 CCAGT ACTGG(1/-1) BssKI 780 CCCGG ^CC[ACGT]GG BstXI 226 CCACTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 648 AGATCT [GA]^GATC[CT] Cfr13I 497 GGGCC G^G[ACGT]CC DraI 402 TTTAAA TTT^AAA EarI 284 CTCTTC CTCTTC(1/4) EcoRI 44 GAATTC G^AATTC EcoT14I 289 CCTTGG C^C[AT][AT]GG Fnu4HI 619 GCTGC GC^[ACGT]GC HaeIII 498 GGCC GG^CC HapII 781 CCGG C^CGG HpaII 781 CCGG C^CGG ItaI 619 GCTGC GC^[ACGT]GC Ksp632I 284 CTCTTC CTCTTC(1/4) MaeI 643 CTAG C^TAG MaeII 136 ACGT A^CGT MaeIII 142 GTAAC ^GT[ACGT]AC MflI 648 AGATCT [GA]^GATC[CT] MslI 227 CACTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 781 CCGG C^CGG MwoI 358 GCAATTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 780 CCCGG CC^[GC]GG RsaI 304 GTAC GT^AC SapI 283 GCTCTTC GCTCTTC(1/4) Sau96I 497 GGGCC G^G[ACGT]CC ScrFI 780 CCCGG CC^[ACGT]GG StyI 289 CCTTGG C^C[AT][AT]GG TaqI 24 TCGA T^CGA TseI 619 GCTGC G^C[AT]GC TthHB8I 24 TCGA T^CGA XhoII 648 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AlwNI 598 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 754 CAGTGGCTG HinfI 705 GAATC G^A[ACGT]TC 717 GATTC MfeI 245 CAATTG C^AATTG 359 CAATTG MnlI 654 CCTC CCTC(7/6) 748 CCTC MseI 311 TTAA T^TAA 403 TTAA MunI 245 CAATTG C^AATTG 359 CAATTG TfiI 705 GAATC G^A[AT]TC 717 GATTC Tru9I 311 TTAA T^TAA 403 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AluI BsiYI BslI BstF5I DdeI DpnII FokI MboI MboII NdeII NlaIII PstI Sau3AI SfaNI SfcI Tsp509I TspRI