################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BmyI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FseI HaeII HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AcsI 102 AAATTC [GA]^AATT[CT] ApoI 102 AAATTC [GA]^AATT[CT] AviII 365 TGCGCA TGC^GCA BbsI 12 GAAGAC GAAGAC(2/6) BcnI 125 CCCGG CC^[GC]GG BglI 284 GCCACTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 42 GCTGAGC GC^T[ACGT]AGC Bpu1102I 42 GCTGAGC GC^T[ACGT]AGC BpuAI 12 GAAGAC GAAGAC(2/6) BsaWI 158 ACCGGA [AT]^CCGG[AT] BsgI 429 CTGCAC GTGCAG(16/14) BsmFI 337 GTCCC GGGAC(10/14) BsrDI 233 GCAATG GCAATG(2/0) BsrI 414 ACTGG ACTGG(1/-1) BstYI 353 AGATCC [GA]^GATC[CT] CelII 42 GCTGAGC GC^T[ACGT]AGC CfoI 366 GCGC GCG^C DraIII 454 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 422 CCGTGG C^C[GA][CT]GG EcoRV 251 GATATC GAT^ATC EcoT14I 217 CCTTGG C^C[AT][AT]GG FspI 365 TGCGCA TGC^GCA HhaI 366 GCGC GCG^C HinP1I 366 GCGC G^CGC HincII 139 GTCAAC GT[CT]^[GA]AC HindII 139 GTCAAC GT[CT]^[GA]AC MaeII 314 ACGT A^CGT MflI 353 AGATCC [GA]^GATC[CT] MslI 229 CACCGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 125 CCCGG CC^[GC]GG PflMI 453 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 3 GACTC GAGTC(4/5) StyI 217 CCTTGG C^C[AT][AT]GG Tsp509I 103 AATT ^AATT Van91I 453 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 353 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 231 CCGC CCGC(-3/-1) 282 CCGC AlwI 206 GATCC GGATC(4/5) 354 GATCC BpmI 49 CTGGAG CTGGAG(16/14) 403 CTGGAG DraII 184 AGGTCCT [GA]G^G[ACGT]CC[CT] 239 AGGACCC EcoO109I 184 AGGTCCT [GA]G^G[ACGT]CC[CT] 239 AGGACCC HaeIII 54 GGCC GG^CC 462 GGCC HphI 214 TCACC GGTGA(8/7) 440 TCACC MaeIII 27 GTCAC ^GT[ACGT]AC 439 GTCAC MboII 12 GAAGA GAAGA(8/7) 408 GAAGA NlaIII 327 CATG CATG^ 457 CATG NlaIV 240 GGACCC GG[ACGT]^[ACGT]CC 372 GGAGCC PpuMI 184 AGGTCCT [GA]G^G[AT]CC[CT] 239 AGGACCC SfaNI 95 GATGC GCATC(5/9) 296 GCATC TfiI 71 GAATC G^A[AT]TC 222 GAATC Tsp45I 27 GTCAC ^GT[GC]AC 439 GTCAC TspRI 29 CACTG CA[GC]TG[ACGT][ACGT]^ 382 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AvaII BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HapII HinfI HpaII ItaI MboI MnlI MspI MvaI MwoI NdeII Sau3AI Sau96I ScrFI TseI