(Circular) (Six-base) MAPSORT of: pFN21AE2284.seq Check: 951 from: 1 to: 7036 pFN21AE2284 7036 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4846 278 Size: 53 83 186 936 3310 2468 Fragments arranged by size: 3310 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4379 1937 Size: 2442 4594 Acc65I G'GTAC_C Cuts at: 4357 4357 Size: 7036 AclI AA'CG_TT Cuts at: 1563 5163 5536 1563 Size: 3600 373 3063 Fragments arranged by size: 3600 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2558 3036 3332 4071 5099 6483 6751 1632 Size: 926 478 296 739 1028 1384 268 1917 Fragments arranged by size: 1917 1384 1028 926 739 478 296 268 AfeI AGC'GCT Cuts at: 2617 2617 Size: 7036 AflII C'TTAA_G Cuts at: 820 1017 4055 820 Size: 197 3038 3801 Fragments arranged by size: 3801 3038 197 AflIII A'CryG_T Cuts at: 1203 5935 6912 1203 Size: 4732 977 1327 Fragments arranged by size: 4732 1327 977 AgeI A'CCGG_T Cuts at: 5867 5867 Size: 7036 AhdI GACnn_n'nnGTC Cuts at: 5765 5765 Size: 7036 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7036 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3043 1184 Size: 1859 5177 AlwNI CAG_nnn'CTG Cuts at: 2339 2806 3156 4779 6351 2339 Size: 467 350 1623 1572 3024 Fragments arranged by size: 3024 1623 1572 467 350 ApaI G_GGCC'C Cuts at: 2105 2992 2105 Size: 887 6149 ApaLI G'TGCA_C Cuts at: 5093 6249 5093 Size: 1156 5880 ApoI r'AATT_y Cuts at: 1493 4345 4571 4723 6832 1493 Size: 2852 226 152 2109 1697 Fragments arranged by size: 2852 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5590 160 Size: 872 4558 1606 Fragments arranged by size: 4558 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7036 AvaI C'yCGr_G Cuts at: 1870 1943 2048 2643 3259 3273 3837 4361 1870 Size: 73 105 595 616 14 564 524 4545 Fragments arranged by size: 4545 616 595 564 524 105 73 14 AvrII C'CTAG_G Cuts at: 3827 3827 Size: 7036 BamHI G'GATC_C Cuts at: 3009 4366 4733 3009 Size: 1357 367 5312 Fragments arranged by size: 5312 1357 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2195 3302 3679 4357 5812 Size: 325 217 627 408 1107 377 678 1455 Cuts at: 5812 618 Size: 1842 Fragments arranged by size: 1842 1455 1107 678 627 408 377 325 217 BanII G_rGCy'C Cuts at: 1391 2045 2105 2992 3489 3620 4286 4355 1391 Size: 654 60 887 497 131 666 69 4072 Fragments arranged by size: 4072 887 666 654 497 131 69 60 BbeI G_GCGC'C Cuts at: 3683 3683 Size: 7036 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6065 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2684 2718 5227 5261 1903 Size: 34 50 34 663 34 2509 34 3678 Fragments arranged by size: 3678 2509 663 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2314 3356 3532 4928 6138 2314 Size: 1042 176 1396 1210 3212 Fragments arranged by size: 3212 1396 1210 1042 176 BclI T'GATC_A Cuts at: 1548 1548 Size: 7036 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2023 2109 2366 Size: 107 122 71 631 605 351 86 257 Cuts at: 2366 2811 5647 136 Size: 445 2836 1525 Fragments arranged by size: 2836 1525 631 605 445 351 257 122 107 86 71 BglII A'GATC_T Cuts at: 7031 7031 Size: 7036 BlpI GC'TnA_GC Cuts at: 3853 4453 3853 Size: 600 6436 Bme1580I G_kGCm'C Cuts at: 1790 2105 2992 4330 5097 6253 1790 Size: 315 887 1338 767 1156 2573 Fragments arranged by size: 2573 1338 1156 887 767 315 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7036 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3654 3675 4767 Size: 416 53 83 399 24 2237 21 1092 Cuts at: 4767 5725 442 Size: 958 1753 Fragments arranged by size: 2237 1753 1092 958 416 399 83 53 24 21 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7036 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3262 3262 Size: 7036 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 2958 3020 3257 3407 5696 1239 Size: 1076 643 62 237 150 2289 2579 Fragments arranged by size: 2579 2289 1076 643 237 150 62 Bpu10I CC'TnA_GC Cuts at: 2673 2673 Size: 7036 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2579 5161 6026 6324 2579 Size: 2582 865 298 3291 Fragments arranged by size: 3291 2582 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2823 5699 882 Size: 617 1324 2876 2219 Fragments arranged by size: 2876 2219 1324 617 BsaAI yAC'GTr Cuts at: 493 1204 4827 493 Size: 711 3623 2702 Fragments arranged by size: 3623 2702 711 BsaBI GATnn'nnATC Cuts at: 7030 7030 Size: 7036 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3680 4230 4843 5225 Size: 53 83 186 936 2147 550 613 382 Cuts at: 5225 5860 275 Size: 635 1451 Fragments arranged by size: 2147 1451 936 635 613 550 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2637 5469 5867 6141 6288 6699 2637 Size: 2832 398 274 147 411 2974 Fragments arranged by size: 2974 2832 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2955 2985 3471 3501 3679 Size: 30 165 30 1552 30 486 30 178 Cuts at: 3679 3709 1178 Size: 30 4505 Fragments arranged by size: 4505 1552 486 178 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 2159 2198 2201 3038 3188 3222 3687 Size: 970 3 39 3 837 150 34 465 Cuts at: 3687 3811 4251 4290 1186 Size: 124 440 39 3932 Fragments arranged by size: 3932 970 837 465 440 150 124 39 39 34 3 3 BseYI C'CCAG_C Cuts at: 3717 3797 4138 6239 6735 3717 Size: 80 341 2101 496 4018 Fragments arranged by size: 4018 2101 496 341 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3230 3764 3906 1163 Size: 573 1494 534 142 4293 Fragments arranged by size: 4293 1494 573 534 142 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4486 5247 6275 1540 Size: 275 186 2485 761 1028 2301 Fragments arranged by size: 2485 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5097 5182 6253 5097 Size: 85 1071 5880 Fragments arranged by size: 5880 1071 85 BsmI GAATG_Cn' Cuts at: 4547 4640 4547 Size: 93 6943 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2105 2992 3489 3620 4286 4330 Size: 399 255 60 887 497 131 666 44 Cuts at: 4330 4355 5097 5182 6253 1391 Size: 25 742 85 1071 2174 Fragments arranged by size: 2174 1071 887 742 666 497 399 255 131 85 60 44 25 BspEI T'CCGG_A Cuts at: 6699 6699 Size: 7036 BspHI T'CATG_A Cuts at: 4925 6655 4925 Size: 1730 5306 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4391 844 Size: 3547 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3852 4923 1394 Size: 429 2029 1071 3507 Fragments arranged by size: 3507 2029 1071 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5531 5705 65 Size: 1183 4283 174 1396 Fragments arranged by size: 4283 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2553 2832 3030 3654 5680 5867 1700 Size: 110 743 279 198 624 2026 187 2869 Fragments arranged by size: 2869 2026 743 624 279 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7036 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7036 BssSI C'ACGA_G Cuts at: 2038 3206 5096 6108 2038 Size: 1168 1890 1012 2966 Fragments arranged by size: 2966 1890 1168 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7036 BstBI TT'CG_AA Cuts at: 5840 5840 Size: 7036 BstEII G'GTnAC_C Cuts at: 1184 2956 3842 1184 Size: 1772 886 4378 Fragments arranged by size: 4378 1772 886 BstYI r'GATC_y Cuts at: 1972 3009 3926 4366 4733 5118 5135 6587 6673 Size: 1037 917 440 367 385 17 1452 86 Cuts at: 6673 6685 7031 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1037 917 440 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2517 4252 2517 Size: 1735 5301 BtgI C'CryG_G Cuts at: 513 1065 2012 2712 3063 3566 4164 513 Size: 552 947 700 351 503 598 3385 Fragments arranged by size: 3385 947 700 598 552 503 351 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6297 Fragments arranged by size: 6297 683 56 BtsI GCAGTG_nn' Cuts at: 2910 3605 3997 4086 4552 5346 5366 2910 Size: 695 392 89 466 794 20 4580 Fragments arranged by size: 4580 794 695 466 392 89 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7036 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7001 DraI TTT'AAA Cuts at: 4340 4340 Size: 7036 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6043 809 Size: 507 4727 1802 Fragments arranged by size: 4727 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2745 2834 2966 Size: 54 1425 50 275 18 915 89 132 Cuts at: 2966 3491 3578 3731 4483 5372 7001 8 Size: 525 87 153 752 889 1629 43 Fragments arranged by size: 1629 1425 915 889 752 525 275 153 132 89 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4483 1537 Size: 275 2671 4090 Fragments arranged by size: 4090 2671 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2085 2133 2151 3055 3885 4966 1353 Size: 732 48 18 904 830 1081 3423 Fragments arranged by size: 3423 1081 904 830 732 48 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5623 5997 6143 387 Size: 1064 138 4034 374 146 1280 Fragments arranged by size: 4034 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 2558 2958 3020 3036 3257 3332 Size: 393 683 243 400 62 16 221 75 Cuts at: 3332 3407 4071 5099 5696 6483 6751 1239 Size: 75 664 1028 597 787 268 1524 Fragments arranged by size: 1524 1028 787 683 664 597 400 393 268 243 221 75 75 62 16 EcoNI CCTnn'n_nnAGG Cuts at: 3826 3837 4291 3826 Size: 11 454 6571 Fragments arranged by size: 6571 454 11 EcoO109I rG'GnC_Cy Cuts at: 2083 2101 2161 2299 2659 2945 2988 3256 3316 Size: 18 60 138 360 286 43 268 60 Cuts at: 3316 3628 3939 4209 2083 Size: 312 311 270 4910 Fragments arranged by size: 4910 360 312 311 286 270 268 138 60 60 43 18 EcoRI G'AATT_C Cuts at: 4345 4723 4345 Size: 378 6658 EcoRV GAT'ATC Cuts at: 3466 3466 Size: 7036 FspI TGC'GCA Cuts at: 5542 5542 Size: 7036 HaeII r_GCGC'y Cuts at: 1678 2619 3683 6183 1678 Size: 941 1064 2500 2531 Fragments arranged by size: 2531 2500 1064 941 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2513 2545 2782 2814 2859 Size: 32 135 32 941 32 237 32 45 Cuts at: 2859 2891 3323 3355 3490 3522 5677 5709 5751 Size: 32 432 32 135 32 2155 32 42 Cuts at: 5751 5783 1373 Size: 32 2626 Fragments arranged by size: 2626 2155 941 432 237 135 135 45 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2496 4380 4626 669 Size: 988 281 558 1884 246 3079 Fragments arranged by size: 3079 1884 988 558 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7036 HpaI GTT'AAC Cuts at: 4626 4626 Size: 7036 KasI G'GCGC_C Cuts at: 3679 3679 Size: 7036 KpnI G_GTAC'C Cuts at: 4361 4361 Size: 7036 MfeI C'AATT_G Cuts at: 4635 4635 Size: 7036 MluI A'CGCG_T Cuts at: 6912 6912 Size: 7036 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3333 3475 6150 6334 1305 Size: 2028 142 2675 184 2007 Fragments arranged by size: 2675 2028 2007 184 142 MscI TGG'CCA Cuts at: 10 64 1489 1832 3733 10 Size: 54 1425 343 1901 3313 Fragments arranged by size: 3313 1901 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4010 4994 5353 5512 518 Size: 648 2844 984 359 159 2042 Fragments arranged by size: 2844 2042 984 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2755 3791 3874 4108 4201 4452 5129 Size: 408 741 1036 83 234 93 251 677 Cuts at: 5129 6277 6522 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1036 741 677 408 251 245 234 93 83 NaeI GCC'GGC Cuts at: 3032 3032 Size: 7036 NarI GG'CG_CC Cuts at: 3680 3680 Size: 7036 NcoI C'CATG_G Cuts at: 513 1065 4164 513 Size: 552 3099 3385 Fragments arranged by size: 3385 3099 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7036 NgoMIV G'CCGG_C Cuts at: 3030 3030 Size: 7036 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7036 NotI GC'GGCC_GC Cuts at: 4483 4483 Size: 7036 NspI r_CATG'y Cuts at: 1132 1229 4394 5939 1132 Size: 97 3165 1545 2229 Fragments arranged by size: 3165 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7036 PciI A'CATG_T Cuts at: 5935 5935 Size: 7036 PflMI CCAn_nnn'nTGG Cuts at: 1108 3299 1108 Size: 2191 4845 PfoI T'CCnGG_A Cuts at: 2997 2997 Size: 7036 PmeI GTTT'AAAC Cuts at: 4340 4340 Size: 7036 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2766 2798 5105 5137 6618 6650 2766 Size: 32 2307 32 1481 32 3152 Fragments arranged by size: 3152 2307 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 2945 3316 3628 4209 2083 Size: 862 371 312 581 4910 Fragments arranged by size: 4910 862 581 371 312 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7036 PsiI TTA'TAA Cuts at: 4606 4606 Size: 7036 PspOMI G'GGCC_C Cuts at: 2101 2988 2101 Size: 887 6149 PstI C_TGCA'G Cuts at: 830 3081 3228 4081 4388 830 Size: 2251 147 853 307 3478 Fragments arranged by size: 3478 2251 853 307 147 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7036 PvuII CAG'CTG Cuts at: 2755 3791 4108 2755 Size: 1036 317 5683 Fragments arranged by size: 5683 1036 317 SacII CC_GC'GG Cuts at: 2015 2015 Size: 7036 SalI G'TCGA_C Cuts at: 1936 4378 1936 Size: 2442 4594 SanDI GG'GwC_CC Cuts at: 2083 3628 2083 Size: 1545 5491 SbfI CC_TGCA'GG Cuts at: 4388 4388 Size: 7036 ScaI AGT'ACT Cuts at: 3664 5284 6996 3664 Size: 1620 1712 3704 Fragments arranged by size: 3704 1712 1620 SfcI C'TryA_G Cuts at: 826 1045 3077 3224 3479 4077 4384 5519 6200 Size: 219 2032 147 255 598 307 1135 681 Cuts at: 6200 6391 826 Size: 191 1471 Fragments arranged by size: 2032 1471 1135 681 598 307 255 219 191 147 SfiI GGCCn_nnn'nGGCC Cuts at: 2109 2109 Size: 7036 SfoI GGC'GCC Cuts at: 3681 3681 Size: 7036 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7036 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7036 SmaI CCC'GGG Cuts at: 1872 2050 2645 3275 4363 1872 Size: 178 595 630 1088 4545 Fragments arranged by size: 4545 1088 630 595 178 SmlI C'TyrA_G Cuts at: 820 1017 1943 2558 3837 4055 5140 6041 6303 Size: 197 926 615 1279 218 1085 901 262 Cuts at: 6303 820 Size: 1553 Fragments arranged by size: 1553 1279 1085 926 901 615 262 218 197 SnaBI TAC'GTA Cuts at: 493 4827 493 Size: 4334 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7036 SphI G_CATG'C Cuts at: 1132 4394 1132 Size: 3262 3774 SspI AAT'ATT Cuts at: 5 52 4960 5 Size: 47 4908 2081 Fragments arranged by size: 4908 2081 47 StuI AGG'CCT Cuts at: 1517 2814 3832 3896 1517 Size: 1297 1018 64 4657 Fragments arranged by size: 4657 1297 1018 64 StyI C'CwwG_G Cuts at: 513 1065 2241 2538 3298 3342 3759 3827 4164 Size: 552 1176 297 760 44 417 68 337 Cuts at: 4164 4475 513 Size: 311 3074 Fragments arranged by size: 3074 1176 760 552 417 337 311 297 68 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5063 5080 5233 5418 5863 325 Size: 1255 3483 17 153 185 445 1498 Fragments arranged by size: 3483 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2488 3662 5282 Size: 274 80 33 51 1443 511 1174 1620 Cuts at: 5282 6994 96 Size: 1712 138 Fragments arranged by size: 1712 1620 1443 1174 511 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4170 4202 6996 7028 4170 Size: 32 2794 32 4178 Fragments arranged by size: 4178 2794 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7036 XbaI T'CTAG_A Cuts at: 4372 4372 Size: 7036 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2719 3349 2719 Size: 630 6406 XhoI C'TCGA_G Cuts at: 1943 3837 1943 Size: 1894 5142 XmaI C'CCGG_G Cuts at: 1870 2048 2643 3273 4361 1870 Size: 178 595 630 1088 4545 Fragments arranged by size: 4545 1088 630 595 178 XmnI GAAnn'nnTTC Cuts at: 2778 5165 2778 Size: 2387 4649 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4844 276 Size: 53 83 186 936 3310 2468 Fragments arranged by size: 3310 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SapI SexAI SrfI SwaI