Restriction map for pFN21AE2284
 (Linear) (Six-base) MAPSORT of: KIEE2284.seq  Check: 8223  from: 1  to: 2331



KIEE2284.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:31  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     553    1031    1327    2066    2331 
   Size:        553     478     296     739     265

  Fragments arranged by size: 

                 739     553     478     296     265
 
AfeI AGC'GCT

Cuts at:      0     612    2331 
   Size:        612    1719
 
AflII C'TTAA_G

Cuts at:      0    2050    2331 
   Size:       2050     281
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1038    2331 
   Size:       1038    1293
 
AlwNI CAG_nnn'CTG

Cuts at:      0     334     801    1151    2331 
   Size:        334     467     350    1180

  Fragments arranged by size: 

                1180     467     350     334
 
ApaI G_GGCC'C

Cuts at:      0     100     987    2331 
   Size:        100     887    1344

  Fragments arranged by size: 

                1344     887     100
 
AvaI C'yCGr_G

Cuts at:      0      43     638    1254    1268    1832    2331 
   Size:         43     595     616      14     564     499

  Fragments arranged by size: 

                 616     595     564     499      43      14
 
AvrII C'CTAG_G

Cuts at:      0    1822    2331 
   Size:       1822     509
 
BamHI G'GATC_C

Cuts at:      0    1004    2331 
   Size:       1004    1327
 
BanI G'GyrC_C

Cuts at:      0     190    1297    1674    2331 
   Size:        190    1107     377     657

  Fragments arranged by size: 

                1107     657     377     190
 
BanII G_rGCy'C

Cuts at:      0      40     100     987    1484    1615    2281    2331 
   Size:         40      60     887     497     131     666      50

  Fragments arranged by size: 

                 887     666     497     131      60      50      40
 
BbeI G_GCGC'C

Cuts at:      0    1678    2331 
   Size:       1678     653
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     679     713    2331 
   Size:        679      34    1618

  Fragments arranged by size: 

                1618     679      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     309    1351    1527    2331 
   Size:        309    1042     176     804

  Fragments arranged by size: 

                1042     804     309     176
 
BglI GCCn_nnn'nGGC

Cuts at:      0      18     104     361     806    2331 
   Size:         18      86     257     445    1525

  Fragments arranged by size: 

                1525     445     257      86      18
 
BlpI GC'TnA_GC

Cuts at:      0    1848    2331 
   Size:       1848     483
 
Bme1580I G_kGCm'C

Cuts at:      0     100     987    2325    2331 
   Size:        100     887    1338       6

  Fragments arranged by size: 

                1338     887     100       6
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1649    1670    2331 
   Size:       1649      21     661

  Fragments arranged by size: 

                1649     661      21
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1257    2331 
   Size:       1257    1074
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     953    1015    1252    1402    2331 
   Size:        310     643      62     237     150     929

  Fragments arranged by size: 

                 929     643     310     237     150      62
 
Bpu10I CC'TnA_GC

Cuts at:      0     668    2331 
   Size:        668    1663
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574    2331 
   Size:        574    1757
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     818    2331 
   Size:        818    1513
 
BsaHI Gr'CG_yC

Cuts at:      0    1675    2225    2331 
   Size:       1675     550     106

  Fragments arranged by size: 

                1675     550     106
 
BsaWI w'CCGG_w

Cuts at:      0     632    2331 
   Size:        632    1699
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     950     980    1466    1496    1674    1704    2331 
   Size:        950      30     486      30     178      30     627

  Fragments arranged by size: 

                 950     627     486     178      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     151     154     193     196    1033    1183    1217    1682 
   Size:        151       3      39       3     837     150      34     465

Cuts at:   1682    1806    2246    2285    2331 
   Size:        124     440      39      46

  Fragments arranged by size: 

                 837     465     440     151     150     124      46      39
                  39      34       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1712    1792    2133    2331 
   Size:       1712      80     341     198

  Fragments arranged by size: 

                1712     341     198      80
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1225    1759    1901    2331 
   Size:       1225     534     142     430

  Fragments arranged by size: 

                1225     534     430     142
 
Bsp1286I G_dGCh'C

Cuts at:      0      40     100     987    1484    1615    2281    2325    2331 
   Size:         40      60     887     497     131     666      44       6

  Fragments arranged by size: 

                 887     666     497     131      60      44      40       6
            
 
BsrBI CCG'CTC

Cuts at:      0    1847    2331 
   Size:       1847     484
 
BsrFI r'CCGG_y

Cuts at:      0     548     827    1025    1649    2331 
   Size:        548     279     198     624     682

  Fragments arranged by size: 

                 682     624     548     279     198
 
BssSI C'ACGA_G

Cuts at:      0      33    1201    2331 
   Size:         33    1168    1130

  Fragments arranged by size: 

                1168    1130      33
 
BstEII G'GTnAC_C

Cuts at:      0     951    1837    2331 
   Size:        951     886     494

  Fragments arranged by size: 

                 951     886     494
 
BstYI r'GATC_y

Cuts at:      0    1004    1921    2331 
   Size:       1004     917     410

  Fragments arranged by size: 

                1004     917     410
 
Bsu36I CC'TnA_GG

Cuts at:      0     512    2247    2331 
   Size:        512    1735      84

  Fragments arranged by size: 

                1735     512      84
 
BtgI C'CryG_G

Cuts at:      0       7     707    1058    1561    2159    2331 
   Size:          7     700     351     503     598     172

  Fragments arranged by size: 

                 700     598     503     351     172       7
 
BtsI GCAGTG_nn'

Cuts at:      0     905    1600    1992    2081    2331 
   Size:        905     695     392      89     250

  Fragments arranged by size: 

                 905     695     392     250      89
 
EaeI y'GGCC_r

Cuts at:      0     740     829     961    1486    1573    1726    2331 
   Size:        740      89     132     525      87     153     605

  Fragments arranged by size: 

                 740     605     525     153     132      89      87
 
EarI CTCTTCn'nnn_

Cuts at:      0      80     128     146    1050    1880    2331 
   Size:         80      48      18     904     830     451

  Fragments arranged by size: 

                 904     830     451      80      48      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     553     953    1015    1031    1252    1327    1402 
   Size:        310     243     400      62      16     221      75      75

Cuts at:   1402    2066    2331 
   Size:        664     265

  Fragments arranged by size: 

                 664     400     310     265     243     221      75      75
                  62      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1821    1832    2286    2331 
   Size:       1821      11     454      45

  Fragments arranged by size: 

                1821     454      45      11
 
EcoO109I rG'GnC_Cy

Cuts at:      0      78      96     156     294     654     940     983    1251 
   Size:         78      18      60     138     360     286      43     268

Cuts at:   1251    1311    1623    1934    2204    2331 
   Size:         60     312     311     270     127

  Fragments arranged by size: 

                 360     312     311     286     270     268     138     127
                  78      60      60      43      18
 
EcoRV GAT'ATC

Cuts at:      0    1461    2331 
   Size:       1461     870
 
HaeII r_GCGC'y

Cuts at:      0     614    1678    2331 
   Size:        614    1064     653

  Fragments arranged by size: 

                1064     653     614
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     508     540     777     809     854     886    1318    1350 
   Size:        508      32     237      32      45      32     432      32

Cuts at:   1350    1485    1517    2331 
   Size:        135      32     814

  Fragments arranged by size: 

                 814     508     432     237     135      45      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     491    2331 
   Size:        491    1840
 
KasI G'GCGC_C

Cuts at:      0    1674    2331 
   Size:       1674     657
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1328    1470    2331 
   Size:       1328     142     861

  Fragments arranged by size: 

                1328     861     142
 
MscI TGG'CCA

Cuts at:      0    1728    2331 
   Size:       1728     603
 
MslI CAynn'nnrTG

Cuts at:      0    2005    2331 
   Size:       2005     326
 
MspA1I CmG'CkG

Cuts at:      0       9     750    1786    1869    2103    2196    2331 
   Size:          9     741    1036      83     234      93     135

  Fragments arranged by size: 

                1036     741     234     135      93      83       9
 
NaeI GCC'GGC

Cuts at:      0    1027    2331 
   Size:       1027    1304
 
NarI GG'CG_CC

Cuts at:      0    1675    2331 
   Size:       1675     656
 
NcoI C'CATG_G

Cuts at:      0    2159    2331 
   Size:       2159     172
 
NgoMIV G'CCGG_C

Cuts at:      0    1025    2331 
   Size:       1025    1306
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1294    2331 
   Size:       1294    1037
 
PfoI T'CCnGG_A

Cuts at:      0     992    2331 
   Size:        992    1339
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     761     793    2331 
   Size:        761      32    1538

  Fragments arranged by size: 

                1538     761      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      78     940    1311    1623    2204    2331 
   Size:         78     862     371     312     581     127

  Fragments arranged by size: 

                 862     581     371     312     127      78
 
PspOMI G'GGCC_C

Cuts at:      0      96     983    2331 
   Size:         96     887    1348

  Fragments arranged by size: 

                1348     887      96
 
PstI C_TGCA'G

Cuts at:      0    1076    1223    2076    2331 
   Size:       1076     147     853     255

  Fragments arranged by size: 

                1076     853     255     147
 
PvuII CAG'CTG

Cuts at:      0     750    1786    2103    2331 
   Size:        750    1036     317     228

  Fragments arranged by size: 

                1036     750     317     228
 
SacII CC_GC'GG

Cuts at:      0      10    2331 
   Size:         10    2321
 
SanDI GG'GwC_CC

Cuts at:      0      78    1623    2331 
   Size:         78    1545     708

  Fragments arranged by size: 

                1545     708      78
 
ScaI AGT'ACT

Cuts at:      0    1659    2331 
   Size:       1659     672
 
SfcI C'TryA_G

Cuts at:      0    1072    1219    1474    2072    2331 
   Size:       1072     147     255     598     259

  Fragments arranged by size: 

                1072     598     259     255     147
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     104    2331 
   Size:        104    2227
 
SfoI GGC'GCC

Cuts at:      0    1676    2331 
   Size:       1676     655
 
SmaI CCC'GGG

Cuts at:      0      45     640    1270    2331 
   Size:         45     595     630    1061

  Fragments arranged by size: 

                1061     630     595      45
 
SmlI C'TyrA_G

Cuts at:      0     553    1832    2050    2331 
   Size:        553    1279     218     281

  Fragments arranged by size: 

                1279     553     281     218
 
StuI AGG'CCT

Cuts at:      0     809    1827    1891    2331 
   Size:        809    1018      64     440

  Fragments arranged by size: 

                1018     809     440      64
 
StyI C'CwwG_G

Cuts at:      0     236     533    1293    1337    1754    1822    2159    2331 
   Size:        236     297     760      44     417      68     337     172

  Fragments arranged by size: 

                 760     417     337     297     236     172      68      44
            
 
TatI w'GTAC_w

Cuts at:      0     483    1657    2331 
   Size:        483    1174     674

  Fragments arranged by size: 

                1174     674     483
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2165    2197    2331 
   Size:       2165      32     134

  Fragments arranged by size: 

                2165     134      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     714    1344    2331 
   Size:        714     630     987

  Fragments arranged by size: 

                 987     714     630
 
XhoI C'TCGA_G

Cuts at:      0    1832    2331 
   Size:       1832     499
 
XmaI C'CCGG_G

Cuts at:      0      43     638    1268    2331 
   Size:         43     595     630    1063

  Fragments arranged by size: 

                1063     630     595      43
 
XmnI GAAnn'nnTTC

Cuts at:      0     773    2331 
   Size:        773    1558
 

 Enzymes that do cut:

     AcuI     AfeI    AflII     AlfI    AlwNI     ApaI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BcgI    BciVI     BglI     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI Bsp1286I    BsrBI    BsrFI
    BssSI   BstEII    BstYI   Bsu36I     BtgI     BtsI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I    EcoRV    HaeII    Hin4I   HincII     KasI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
    PflMI     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII    SacII
    SanDI     ScaI     SfcI     SfiI     SfoI     SmaI     SmlI     StuI
     StyI     TatI     TstI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI   AflIII     AgeI     AhdI
     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI
     BbsI    BbvCI     BclI    BfrBI    BglII    BmgBI     BmtI    BsaAI
    BsaBI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrGI   BssHII   BstAPI    BstBI    BstXI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoRI     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI     SalI     SapI     SbfI
    SexAI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII  Tth111I     XbaI     ZraI
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