(Linear) (Six-base) MAPSORT of: KIEE2284.seq Check: 8223 from: 1 to: 2331 KIEE2284.orf With 182 enzymes: SgfI * December 27, 2011 16:31 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 553 1031 1327 2066 2331 Size: 553 478 296 739 265 Fragments arranged by size: 739 553 478 296 265 AfeI AGC'GCT Cuts at: 0 612 2331 Size: 612 1719 AflII C'TTAA_G Cuts at: 0 2050 2331 Size: 2050 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1038 2331 Size: 1038 1293 AlwNI CAG_nnn'CTG Cuts at: 0 334 801 1151 2331 Size: 334 467 350 1180 Fragments arranged by size: 1180 467 350 334 ApaI G_GGCC'C Cuts at: 0 100 987 2331 Size: 100 887 1344 Fragments arranged by size: 1344 887 100 AvaI C'yCGr_G Cuts at: 0 43 638 1254 1268 1832 2331 Size: 43 595 616 14 564 499 Fragments arranged by size: 616 595 564 499 43 14 AvrII C'CTAG_G Cuts at: 0 1822 2331 Size: 1822 509 BamHI G'GATC_C Cuts at: 0 1004 2331 Size: 1004 1327 BanI G'GyrC_C Cuts at: 0 190 1297 1674 2331 Size: 190 1107 377 657 Fragments arranged by size: 1107 657 377 190 BanII G_rGCy'C Cuts at: 0 40 100 987 1484 1615 2281 2331 Size: 40 60 887 497 131 666 50 Fragments arranged by size: 887 666 497 131 60 50 40 BbeI G_GCGC'C Cuts at: 0 1678 2331 Size: 1678 653 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 679 713 2331 Size: 679 34 1618 Fragments arranged by size: 1618 679 34 BciVI GTATCCnnnnn_n' Cuts at: 0 309 1351 1527 2331 Size: 309 1042 176 804 Fragments arranged by size: 1042 804 309 176 BglI GCCn_nnn'nGGC Cuts at: 0 18 104 361 806 2331 Size: 18 86 257 445 1525 Fragments arranged by size: 1525 445 257 86 18 BlpI GC'TnA_GC Cuts at: 0 1848 2331 Size: 1848 483 Bme1580I G_kGCm'C Cuts at: 0 100 987 2325 2331 Size: 100 887 1338 6 Fragments arranged by size: 1338 887 100 6 BmrI ACTGGGnnnn_n' Cuts at: 0 1649 1670 2331 Size: 1649 21 661 Fragments arranged by size: 1649 661 21 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1257 2331 Size: 1257 1074 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 953 1015 1252 1402 2331 Size: 310 643 62 237 150 929 Fragments arranged by size: 929 643 310 237 150 62 Bpu10I CC'TnA_GC Cuts at: 0 668 2331 Size: 668 1663 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 574 2331 Size: 574 1757 BsaI GGTCTCn'nnnn_ Cuts at: 0 818 2331 Size: 818 1513 BsaHI Gr'CG_yC Cuts at: 0 1675 2225 2331 Size: 1675 550 106 Fragments arranged by size: 1675 550 106 BsaWI w'CCGG_w Cuts at: 0 632 2331 Size: 632 1699 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 950 980 1466 1496 1674 1704 2331 Size: 950 30 486 30 178 30 627 Fragments arranged by size: 950 627 486 178 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 151 154 193 196 1033 1183 1217 1682 Size: 151 3 39 3 837 150 34 465 Cuts at: 1682 1806 2246 2285 2331 Size: 124 440 39 46 Fragments arranged by size: 837 465 440 151 150 124 46 39 39 34 3 3 BseYI C'CCAG_C Cuts at: 0 1712 1792 2133 2331 Size: 1712 80 341 198 Fragments arranged by size: 1712 341 198 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1225 1759 1901 2331 Size: 1225 534 142 430 Fragments arranged by size: 1225 534 430 142 Bsp1286I G_dGCh'C Cuts at: 0 40 100 987 1484 1615 2281 2325 2331 Size: 40 60 887 497 131 666 44 6 Fragments arranged by size: 887 666 497 131 60 44 40 6 BsrBI CCG'CTC Cuts at: 0 1847 2331 Size: 1847 484 BsrFI r'CCGG_y Cuts at: 0 548 827 1025 1649 2331 Size: 548 279 198 624 682 Fragments arranged by size: 682 624 548 279 198 BssSI C'ACGA_G Cuts at: 0 33 1201 2331 Size: 33 1168 1130 Fragments arranged by size: 1168 1130 33 BstEII G'GTnAC_C Cuts at: 0 951 1837 2331 Size: 951 886 494 Fragments arranged by size: 951 886 494 BstYI r'GATC_y Cuts at: 0 1004 1921 2331 Size: 1004 917 410 Fragments arranged by size: 1004 917 410 Bsu36I CC'TnA_GG Cuts at: 0 512 2247 2331 Size: 512 1735 84 Fragments arranged by size: 1735 512 84 BtgI C'CryG_G Cuts at: 0 7 707 1058 1561 2159 2331 Size: 7 700 351 503 598 172 Fragments arranged by size: 700 598 503 351 172 7 BtsI GCAGTG_nn' Cuts at: 0 905 1600 1992 2081 2331 Size: 905 695 392 89 250 Fragments arranged by size: 905 695 392 250 89 EaeI y'GGCC_r Cuts at: 0 740 829 961 1486 1573 1726 2331 Size: 740 89 132 525 87 153 605 Fragments arranged by size: 740 605 525 153 132 89 87 EarI CTCTTCn'nnn_ Cuts at: 0 80 128 146 1050 1880 2331 Size: 80 48 18 904 830 451 Fragments arranged by size: 904 830 451 80 48 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 553 953 1015 1031 1252 1327 1402 Size: 310 243 400 62 16 221 75 75 Cuts at: 1402 2066 2331 Size: 664 265 Fragments arranged by size: 664 400 310 265 243 221 75 75 62 16 EcoNI CCTnn'n_nnAGG Cuts at: 0 1821 1832 2286 2331 Size: 1821 11 454 45 Fragments arranged by size: 1821 454 45 11 EcoO109I rG'GnC_Cy Cuts at: 0 78 96 156 294 654 940 983 1251 Size: 78 18 60 138 360 286 43 268 Cuts at: 1251 1311 1623 1934 2204 2331 Size: 60 312 311 270 127 Fragments arranged by size: 360 312 311 286 270 268 138 127 78 60 60 43 18 EcoRV GAT'ATC Cuts at: 0 1461 2331 Size: 1461 870 HaeII r_GCGC'y Cuts at: 0 614 1678 2331 Size: 614 1064 653 Fragments arranged by size: 1064 653 614 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 508 540 777 809 854 886 1318 1350 Size: 508 32 237 32 45 32 432 32 Cuts at: 1350 1485 1517 2331 Size: 135 32 814 Fragments arranged by size: 814 508 432 237 135 45 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 491 2331 Size: 491 1840 KasI G'GCGC_C Cuts at: 0 1674 2331 Size: 1674 657 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1328 1470 2331 Size: 1328 142 861 Fragments arranged by size: 1328 861 142 MscI TGG'CCA Cuts at: 0 1728 2331 Size: 1728 603 MslI CAynn'nnrTG Cuts at: 0 2005 2331 Size: 2005 326 MspA1I CmG'CkG Cuts at: 0 9 750 1786 1869 2103 2196 2331 Size: 9 741 1036 83 234 93 135 Fragments arranged by size: 1036 741 234 135 93 83 9 NaeI GCC'GGC Cuts at: 0 1027 2331 Size: 1027 1304 NarI GG'CG_CC Cuts at: 0 1675 2331 Size: 1675 656 NcoI C'CATG_G Cuts at: 0 2159 2331 Size: 2159 172 NgoMIV G'CCGG_C Cuts at: 0 1025 2331 Size: 1025 1306 PflMI CCAn_nnn'nTGG Cuts at: 0 1294 2331 Size: 1294 1037 PfoI T'CCnGG_A Cuts at: 0 992 2331 Size: 992 1339 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 761 793 2331 Size: 761 32 1538 Fragments arranged by size: 1538 761 32 PpuMI rG'GwC_Cy Cuts at: 0 78 940 1311 1623 2204 2331 Size: 78 862 371 312 581 127 Fragments arranged by size: 862 581 371 312 127 78 PspOMI G'GGCC_C Cuts at: 0 96 983 2331 Size: 96 887 1348 Fragments arranged by size: 1348 887 96 PstI C_TGCA'G Cuts at: 0 1076 1223 2076 2331 Size: 1076 147 853 255 Fragments arranged by size: 1076 853 255 147 PvuII CAG'CTG Cuts at: 0 750 1786 2103 2331 Size: 750 1036 317 228 Fragments arranged by size: 1036 750 317 228 SacII CC_GC'GG Cuts at: 0 10 2331 Size: 10 2321 SanDI GG'GwC_CC Cuts at: 0 78 1623 2331 Size: 78 1545 708 Fragments arranged by size: 1545 708 78 ScaI AGT'ACT Cuts at: 0 1659 2331 Size: 1659 672 SfcI C'TryA_G Cuts at: 0 1072 1219 1474 2072 2331 Size: 1072 147 255 598 259 Fragments arranged by size: 1072 598 259 255 147 SfiI GGCCn_nnn'nGGCC Cuts at: 0 104 2331 Size: 104 2227 SfoI GGC'GCC Cuts at: 0 1676 2331 Size: 1676 655 SmaI CCC'GGG Cuts at: 0 45 640 1270 2331 Size: 45 595 630 1061 Fragments arranged by size: 1061 630 595 45 SmlI C'TyrA_G Cuts at: 0 553 1832 2050 2331 Size: 553 1279 218 281 Fragments arranged by size: 1279 553 281 218 StuI AGG'CCT Cuts at: 0 809 1827 1891 2331 Size: 809 1018 64 440 Fragments arranged by size: 1018 809 440 64 StyI C'CwwG_G Cuts at: 0 236 533 1293 1337 1754 1822 2159 2331 Size: 236 297 760 44 417 68 337 172 Fragments arranged by size: 760 417 337 297 236 172 68 44 TatI w'GTAC_w Cuts at: 0 483 1657 2331 Size: 483 1174 674 Fragments arranged by size: 1174 674 483 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2165 2197 2331 Size: 2165 32 134 Fragments arranged by size: 2165 134 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 714 1344 2331 Size: 714 630 987 Fragments arranged by size: 987 714 630 XhoI C'TCGA_G Cuts at: 0 1832 2331 Size: 1832 499 XmaI C'CCGG_G Cuts at: 0 43 638 1268 2331 Size: 43 595 630 1063 Fragments arranged by size: 1063 630 595 43 XmnI GAAnn'nnTTC Cuts at: 0 773 2331 Size: 773 1558 Enzymes that do cut: AcuI AfeI AflII AlfI AlwNI ApaI AvaI AvrII BamHI BanI BanII BbeI BcgI BciVI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI Bsp1286I BsrBI BsrFI BssSI BstEII BstYI Bsu36I BtgI BtsI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacII SanDI ScaI SfcI SfiI SfoI SmaI SmlI StuI StyI TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflIII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI BaeI BbsI BbvCI BclI BfrBI BglII BmgBI BmtI BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SapI SbfI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI ZraI