################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfcI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflIII 195 ACGTGT A^C[GA][CT]GT AluI 317 AGCT AG^CT ApaI 353 GGGCCC GGGCC^C AvaII 291 GGTCC G^G[AT]CC BanII 353 GGGCCC G[GA]GC[CT]^C BbsI 325 GAAGAC GAAGAC(2/6) BbvI 366 GCTGC GCAGC(8/12) BclI 200 TGATCA T^GATCA BglI 419 GCCGCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 353 GGGCCC G[AGT]GC[ACT]^C BpmI 331 CTGGAG CTGGAG(16/14) BpuAI 325 GAAGAC GAAGAC(2/6) BsmAI 3 GTCTC GTCTC(1/5) Bsp1286I 353 GGGCCC G[AGT]GC[ACT]^C BspDI 58 ATCGAT AT^CGAT BstAPI 30 GCATATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 299 CCAATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 233 AGATCC [GA]^GATC[CT] Cac8I 398 GCCAGC GC[ACGT]^[ACGT]GC ClaI 58 ATCGAT AT^CGAT DdeI 179 CTGAG C^T[ACGT]AG FauI 166 GCGGG CCCGC(4/6) FbaI 200 TGATCA T^GATCA HgaI 369 GCGTC GACGC(5/10) MaeII 195 ACGT A^CGT MaeIII 371 GTCAC ^GT[ACGT]AC MflI 233 AGATCC [GA]^GATC[CT] MseI 98 TTAA T^TAA MslI 204 CACCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIII 124 CATG CATG^ PpuMI 290 AGGTCCT [GA]G^G[AT]CC[CT] ScaI 42 AGTACT AGT^ACT SfiI 418 GGCCGCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TaqI 59 TCGA T^CGA Tru9I 98 TTAA T^TAA TseI 366 GCTGC G^C[AT]GC Tsp45I 371 GTCAC ^GT[GC]AC TthHB8I 59 TCGA T^CGA XcmI 95 CCATTAAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 233 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 43 GTAC GT^AC 228 GTAC AlwI 12 GATCC GGATC(4/5) 234 GATCC BfaI 7 CTAG C^TAG 295 CTAG BsiYI 356 CCCATAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 429 CCGCACCGTGG BslI 356 CCCATAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 429 CCGCACCGTGG BssKI 288 CCAGG ^CC[ACGT]GG 330 CCTGG BstNI 288 CCAGG CC^[AT]GG 330 CCTGG DraII 290 AGGTCCT [GA]G^G[ACGT]CC[CT] 380 GGGGCCT DsaI 423 CCGTGG C^C[GA][CT]GG 434 CCGTGG EaeI 417 TGGCCG [CT]^GGCC[GA] 426 TGGCCG EcoO109I 290 AGGTCCT [GA]G^G[ACGT]CC[CT] 380 GGGGCCT EcoRII 288 CCAGG ^CC[AT]GG 330 CCTGG HinfI 216 GAATC G^A[ACGT]TC 348 GATTC HphI 155 TCACC GGTGA(8/7) 203 TCACC MaeI 7 CTAG C^TAG 295 CTAG MvaI 288 CCAGG CC^[AT]GG 330 CCTGG NlaIV 353 GGGCCC GG[ACGT]^[ACGT]CC 380 GGGGCC RsaI 43 GTAC GT^AC 228 GTAC ScrFI 288 CCAGG CC^[ACGT]GG 330 CCTGG TfiI 216 GAATC G^A[AT]TC 348 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AciI BsaJI BsrI BstF5I Cfr13I DpnII Fnu4HI FokI HaeIII ItaI MboI MboII MnlI MwoI NdeII Sau3AI Sau96I TspRI