Restriction map for pFN21AE1709
 (Circular) (Six-base) MAPSORT of: pFN21AE1709.seq  Check: 8127  from: 1  to: 5350



pFN21AE1709  5350 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:50  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3160     278 
   Size:         53      83     186     936    1624    2468

  Fragments arranged by size: 

                2468    1624     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2693    1937 
   Size:        756    4594
 
Acc65I G'GTAC_C

Cuts at:   2671    2671 
   Size:       5350
 
AclI AA'CG_TT

Cuts at:   1563    3477    3850    1563 
   Size:       1914     373    3063

  Fragments arranged by size: 

                3063    1914     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3413    4797    5065    1632 
   Size:       1781    1384     268    1917

  Fragments arranged by size: 

                1917    1781    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5153
 
AflIII A'CryG_T

Cuts at:   1203    4249    5226    1203 
   Size:       3046     977    1327

  Fragments arranged by size: 

                3046    1327     977
 
AgeI A'CCGG_T

Cuts at:   4181    4181 
   Size:       5350
 
AhdI GACnn_n'nnGTC

Cuts at:   4079    4079 
   Size:       5350
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5350
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5350
 
AlwNI CAG_nnn'CTG

Cuts at:   3093    4665    3093 
   Size:       1572    3778
 
ApaLI G'TGCA_C

Cuts at:   3407    4563    3407 
   Size:       1156    4194
 
ApoI r'AATT_y

Cuts at:   1493    2063    2102    2659    2885    3037    5146    1493 
   Size:        570      39     557     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     570     557     226     152      39
 
AseI AT'TA_AT

Cuts at:    160    1032    3904     160 
   Size:        872    2872    1606

  Fragments arranged by size: 

                2872    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5350
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2519    2675    1870 
   Size:         73     576     156    4545

  Fragments arranged by size: 

                4545     576     156      73
 
BamHI G'GATC_C

Cuts at:   2680    3047    2680 
   Size:        367    4983
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2471    2671    4126     618 
   Size:        325     217     627     684     200    1455    1842

  Fragments arranged by size: 

                1842    1455     684     627     325     217     200
 
BanII G_rGCy'C

Cuts at:   1391    2052    2633    2669    1391 
   Size:        661     581      36    4072

  Fragments arranged by size: 

                4072     661     581      36
 
BbeI G_GCGC'C

Cuts at:   2475    2475 
   Size:       5350
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2091    2199     928 
   Size:        971     192     108    4079

  Fragments arranged by size: 

                4079     971     192     108
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3541    3575    1903 
   Size:         34    1604      34    3678

  Fragments arranged by size: 

                3678    1604      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3242    4452    3242 
   Size:       1210    4140
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5350
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3961     136 
   Size:        107     122      71     631     605    2289    1525

  Fragments arranged by size: 

                2289    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5345    5345 
   Size:       5350
 
BlpI GC'TnA_GC

Cuts at:   2767    2767 
   Size:       5350
 
Bme1580I G_kGCm'C

Cuts at:   1790    2559    3411    4567    1790 
   Size:        769     852    1156    2573

  Fragments arranged by size: 

                2573    1156     852     769
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5350
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2627    3081    4039 
   Size:        416      53      83     399      24    1210     454     958

Cuts at:   4039     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1210     958     454     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5350
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2192    4010    1239 
   Size:        953    1818    2579

  Fragments arranged by size: 

                2579    1818     953
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3475    4340    4638    3475 
   Size:        865     298    4187

  Fragments arranged by size: 

                4187     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2530    4013     882 
   Size:        617    1031    1483    2219

  Fragments arranged by size: 

                2219    1483    1031     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3141     493 
   Size:        711    1937    2702

  Fragments arranged by size: 

                2702    1937     711
 
BsaBI GATnn'nnATC

Cuts at:   5344    5344 
   Size:       5350
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2472    3157    3539    4174 
   Size:         53      83     186     936     939     685     382     635

Cuts at:   4174     275 
   Size:       1451

  Fragments arranged by size: 

                1451     939     936     685     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3783    4181    4455    4602    5013    3783 
   Size:        398     274     147     411    4120

  Fragments arranged by size: 

                4120     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5125

  Fragments arranged by size: 

                5125     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2033    1186 
   Size:        847    4503
 
BseYI C'CCAG_C

Cuts at:   4553    5049    4553 
   Size:        496    4854
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4777
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2800    3561    4589    1540 
   Size:        275     186     799     761    1028    2301

  Fragments arranged by size: 

                2301    1028     799     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3411    3496    4567    3411 
   Size:         85    1071    4194

  Fragments arranged by size: 

                4194    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2861    2954    2861 
   Size:         93    5257
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2052    2559    2633    2669    3411    3496    4567 
   Size:        399     262     507      74      36     742      85    1071

Cuts at:   4567    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     507     399     262      85      74
                  36
 
BspEI T'CCGG_A

Cuts at:   5013    5013 
   Size:       5350
 
BspHI T'CATG_A

Cuts at:   3239    4969    3239 
   Size:       1730    3620
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2119    2548    2705     844 
   Size:       1275     429     157    3489

  Fragments arranged by size: 

                3489    1275     429     157
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3237    1394 
   Size:        429    1414    3507

  Fragments arranged by size: 

                3507    1414     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2065    3845    4019      65 
   Size:       1183     817    1780     174    1396

  Fragments arranged by size: 

                1780    1396    1183     817     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2526    3994    4181    1700 
   Size:        110     716    1468     187    2869

  Fragments arranged by size: 

                2869    1468     716     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2433      96 
   Size:       2337    3013
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5350
 
BssSI C'ACGA_G

Cuts at:   2334    3410    4422    2334 
   Size:       1076    1012    3262

  Fragments arranged by size: 

                3262    1076    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5350
 
BstBI TT'CG_AA

Cuts at:   4154    4154 
   Size:       5350
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5350
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2425    2425 
   Size:       5350
 
BstYI r'GATC_y

Cuts at:   1972    2577    2680    3047    3432    3449    4901    4987    4999 
   Size:        605     103     367     385      17    1452      86      12

Cuts at:   4999    5345    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     605     385     367     346     103      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2053    2278    2397     513 
   Size:        552     988     225     119    3466

  Fragments arranged by size: 

                3466     988     552     225     119
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2269     487 
   Size:        683      56    1043    3568

  Fragments arranged by size: 

                3568    1043     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2866    3660    3680    2866 
   Size:        794      20    4536

  Fragments arranged by size: 

                4536     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5350
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5315
 
DraI TTT'AAA

Cuts at:   2654    2654 
   Size:       5350
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4357     809 
   Size:        507    3041    1802

  Fragments arranged by size: 

                3041    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2797    3686    5315 
   Size:         54    1425      50     275      18     967     889    1629

Cuts at:   5315       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     967     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2797    1537 
   Size:        275     985    4090

  Fragments arranged by size: 

                4090     985     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2510    3280    1353 
   Size:       1157     770    3423

  Fragments arranged by size: 

                3423    1157     770
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3937    4311    4457     387 
   Size:       1064     138    2348     374     146    1280

  Fragments arranged by size: 

                2348    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2192    3413    4010    4797    5065    1239 
   Size:        393     560    1221     597     787     268    1524

  Fragments arranged by size: 

                1524    1221     787     597     560     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2131    2131 
   Size:       5350
 
EcoRI G'AATT_C

Cuts at:   2063    2659    3037    2063 
   Size:        596     378    4376

  Fragments arranged by size: 

                4376     596     378
 
FspI TGC'GCA

Cuts at:   3856    3856 
   Size:       5350
 
HaeII r_GCGC'y

Cuts at:   1678    2475    4497    1678 
   Size:        797    2022    2531

  Fragments arranged by size: 

                2531    2022     797
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2009    2041    3991    4023    4065 
   Size:         32     135      32     437      32    1950      32      42

Cuts at:   4065    4097    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1950     437     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2694    2940     669 
   Size:        988     281     756     246    3079

  Fragments arranged by size: 

                3079     988     756     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5350
 
HpaI GTT'AAC

Cuts at:   2940    2940 
   Size:       5350
 
KasI G'GCGC_C

Cuts at:   2471    2471 
   Size:       5350
 
KpnI G_GTAC'C

Cuts at:   2675    2675 
   Size:       5350
 
MfeI C'AATT_G

Cuts at:   2949    2949 
   Size:       5350
 
MluI A'CGCG_T

Cuts at:   5226    5226 
   Size:       5350
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2277    4464    4648    1305 
   Size:        972    2187     184    2007

  Fragments arranged by size: 

                2187    2007     972     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3528

  Fragments arranged by size: 

                3528    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2429    2466    3308    3667    3826     518 
   Size:        648    1263      37     842     359     159    2042

  Fragments arranged by size: 

                2042    1263     842     648     359     159      37
 
MspA1I CmG'CkG

Cuts at:   1606    2599    2766    3443    4591    4836    1606 
   Size:        993     167     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     993     677     245     167
 
NarI GG'CG_CC

Cuts at:   2472    2472 
   Size:       5350
 
NcoI C'CATG_G

Cuts at:    513    1065    2397     513 
   Size:        552    1332    3466

  Fragments arranged by size: 

                3466    1332     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5350
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5350
 
NotI GC'GGCC_GC

Cuts at:   2797    2797 
   Size:       5350
 
NspI r_CATG'y

Cuts at:   1132    1229    2503    2708    4253    1132 
   Size:         97    1274     205    1545    2229

  Fragments arranged by size: 

                2229    1545    1274     205      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5350
 
PciI A'CATG_T

Cuts at:   4249    4249 
   Size:       5350
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2296    2587    1108 
   Size:       1188     291    3871

  Fragments arranged by size: 

                3871    1188     291
 
PfoI T'CCnGG_A

Cuts at:   2027    2094    2027 
   Size:         67    5283
 
PmeI GTTT'AAAC

Cuts at:   2654    2654 
   Size:       5350
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3419    3451    4932    4964    3419 
   Size:         32    1481      32    3805

  Fragments arranged by size: 

                3805    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2131    2131 
   Size:       5350
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5350
 
PsiI TTA'TAA

Cuts at:   2920    2920 
   Size:       5350
 
PstI C_TGCA'G

Cuts at:    830    2604    2702     830 
   Size:       1774      98    3478

  Fragments arranged by size: 

                3478    1774      98
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5350
 
PvuII CAG'CTG

Cuts at:   2599    2599 
   Size:       5350
 
SalI G'TCGA_C

Cuts at:   1936    2692    1936 
   Size:        756    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2510    2510 
   Size:       5350
 
SbfI CC_TGCA'GG

Cuts at:   2702    2702 
   Size:       5350
 
ScaI AGT'ACT

Cuts at:   3598    5310    3598 
   Size:       1712    3638
 
SfcI C'TryA_G

Cuts at:    826    1045    2600    2698    3833    4514    4705     826 
   Size:        219    1555      98    1135     681     191    1471

  Fragments arranged by size: 

                1555    1471    1135     681     219     191      98
 
SfoI GGC'GCC

Cuts at:   2473    2473 
   Size:       5350
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5350
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5350
 
SmaI CCC'GGG

Cuts at:   1872    2677    1872 
   Size:        805    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3454    4355    4617     820 
   Size:        197     926    1511     901     262    1553

  Fragments arranged by size: 

                1553    1511     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3141     493 
   Size:       2648    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5350
 
SphI G_CATG'C

Cuts at:   1132    2708    1132 
   Size:       1576    3774
 
SspI AAT'ATT

Cuts at:      5      52    3274       5 
   Size:         47    3222    2081

  Fragments arranged by size: 

                3222    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5350
 
StyI C'CwwG_G

Cuts at:    513    1065    2397    2789     513 
   Size:        552    1332     392    3074

  Fragments arranged by size: 

                3074    1332     552     392
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2455    3377    3394    3547    3732    4177     325 
   Size:       1255     875     922      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255     922     875     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2340    2433    3596 
   Size:        274      80      33      51    1443     363      93    1163

Cuts at:   3596    5308      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1163     363     274     138      93      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5310    5342    5310 
   Size:         32    5318
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5350
 
XbaI T'CTAG_A

Cuts at:   2152    2686    2152 
   Size:        534    4816
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2465    2465 
   Size:       5350
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5350
 
XmaI C'CCGG_G

Cuts at:   1870    2675    1870 
   Size:        805    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3479    3479 
   Size:       5350
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3158     276 
   Size:         53      83     186     936    1624    2468

  Fragments arranged by size: 

                2468    1624     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
     PstI     PvuI    PvuII     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     NaeI   NgoMIV
     NruI     NsiI     PacI     PmlI   PspOMI    PspXI     PsrI    RsrII
     SacI    SacII    SanDI    SexAI     SfiI     SrfI     SwaI
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