(Circular) (Six-base) MAPSORT of: pFN21AE1709.seq Check: 8127 from: 1 to: 5350 pFN21AE1709 5350 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3160 278 Size: 53 83 186 936 1624 2468 Fragments arranged by size: 2468 1624 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2693 1937 Size: 756 4594 Acc65I G'GTAC_C Cuts at: 2671 2671 Size: 5350 AclI AA'CG_TT Cuts at: 1563 3477 3850 1563 Size: 1914 373 3063 Fragments arranged by size: 3063 1914 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3413 4797 5065 1632 Size: 1781 1384 268 1917 Fragments arranged by size: 1917 1781 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5153 AflIII A'CryG_T Cuts at: 1203 4249 5226 1203 Size: 3046 977 1327 Fragments arranged by size: 3046 1327 977 AgeI A'CCGG_T Cuts at: 4181 4181 Size: 5350 AhdI GACnn_n'nnGTC Cuts at: 4079 4079 Size: 5350 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5350 AlwNI CAG_nnn'CTG Cuts at: 3093 4665 3093 Size: 1572 3778 ApaLI G'TGCA_C Cuts at: 3407 4563 3407 Size: 1156 4194 ApoI r'AATT_y Cuts at: 1493 2063 2102 2659 2885 3037 5146 1493 Size: 570 39 557 226 152 2109 1697 Fragments arranged by size: 2109 1697 570 557 226 152 39 AseI AT'TA_AT Cuts at: 160 1032 3904 160 Size: 872 2872 1606 Fragments arranged by size: 2872 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5350 AvaI C'yCGr_G Cuts at: 1870 1943 2519 2675 1870 Size: 73 576 156 4545 Fragments arranged by size: 4545 576 156 73 BamHI G'GATC_C Cuts at: 2680 3047 2680 Size: 367 4983 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2471 2671 4126 618 Size: 325 217 627 684 200 1455 1842 Fragments arranged by size: 1842 1455 684 627 325 217 200 BanII G_rGCy'C Cuts at: 1391 2052 2633 2669 1391 Size: 661 581 36 4072 Fragments arranged by size: 4072 661 581 36 BbeI G_GCGC'C Cuts at: 2475 2475 Size: 5350 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2091 2199 928 Size: 971 192 108 4079 Fragments arranged by size: 4079 971 192 108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3541 3575 1903 Size: 34 1604 34 3678 Fragments arranged by size: 3678 1604 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3242 4452 3242 Size: 1210 4140 BclI T'GATC_A Cuts at: 1548 1548 Size: 5350 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3961 136 Size: 107 122 71 631 605 2289 1525 Fragments arranged by size: 2289 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5345 5345 Size: 5350 BlpI GC'TnA_GC Cuts at: 2767 2767 Size: 5350 Bme1580I G_kGCm'C Cuts at: 1790 2559 3411 4567 1790 Size: 769 852 1156 2573 Fragments arranged by size: 2573 1156 852 769 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5350 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2627 3081 4039 Size: 416 53 83 399 24 1210 454 958 Cuts at: 4039 442 Size: 1753 Fragments arranged by size: 1753 1210 958 454 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5350 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 4010 1239 Size: 953 1818 2579 Fragments arranged by size: 2579 1818 953 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3475 4340 4638 3475 Size: 865 298 4187 Fragments arranged by size: 4187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2530 4013 882 Size: 617 1031 1483 2219 Fragments arranged by size: 2219 1483 1031 617 BsaAI yAC'GTr Cuts at: 493 1204 3141 493 Size: 711 1937 2702 Fragments arranged by size: 2702 1937 711 BsaBI GATnn'nnATC Cuts at: 5344 5344 Size: 5350 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2472 3157 3539 4174 Size: 53 83 186 936 939 685 382 635 Cuts at: 4174 275 Size: 1451 Fragments arranged by size: 1451 939 936 685 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3783 4181 4455 4602 5013 3783 Size: 398 274 147 411 4120 Fragments arranged by size: 4120 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5125 Fragments arranged by size: 5125 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 1186 Size: 847 4503 BseYI C'CCAG_C Cuts at: 4553 5049 4553 Size: 496 4854 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4777 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2800 3561 4589 1540 Size: 275 186 799 761 1028 2301 Fragments arranged by size: 2301 1028 799 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3411 3496 4567 3411 Size: 85 1071 4194 Fragments arranged by size: 4194 1071 85 BsmI GAATG_Cn' Cuts at: 2861 2954 2861 Size: 93 5257 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2559 2633 2669 3411 3496 4567 Size: 399 262 507 74 36 742 85 1071 Cuts at: 4567 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 507 399 262 85 74 36 BspEI T'CCGG_A Cuts at: 5013 5013 Size: 5350 BspHI T'CATG_A Cuts at: 3239 4969 3239 Size: 1730 3620 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2119 2548 2705 844 Size: 1275 429 157 3489 Fragments arranged by size: 3489 1275 429 157 BsrBI CCG'CTC Cuts at: 1394 1823 3237 1394 Size: 429 1414 3507 Fragments arranged by size: 3507 1414 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2065 3845 4019 65 Size: 1183 817 1780 174 1396 Fragments arranged by size: 1780 1396 1183 817 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2526 3994 4181 1700 Size: 110 716 1468 187 2869 Fragments arranged by size: 2869 1468 716 187 110 BsrGI T'GTAC_A Cuts at: 96 2433 96 Size: 2337 3013 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5350 BssSI C'ACGA_G Cuts at: 2334 3410 4422 2334 Size: 1076 1012 3262 Fragments arranged by size: 3262 1076 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5350 BstBI TT'CG_AA Cuts at: 4154 4154 Size: 5350 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5350 BstXI CCAn_nnnn'nTGG Cuts at: 2425 2425 Size: 5350 BstYI r'GATC_y Cuts at: 1972 2577 2680 3047 3432 3449 4901 4987 4999 Size: 605 103 367 385 17 1452 86 12 Cuts at: 4999 5345 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 605 385 367 346 103 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2053 2278 2397 513 Size: 552 988 225 119 3466 Fragments arranged by size: 3466 988 552 225 119 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2269 487 Size: 683 56 1043 3568 Fragments arranged by size: 3568 1043 683 56 BtsI GCAGTG_nn' Cuts at: 2866 3660 3680 2866 Size: 794 20 4536 Fragments arranged by size: 4536 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5350 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5315 DraI TTT'AAA Cuts at: 2654 2654 Size: 5350 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4357 809 Size: 507 3041 1802 Fragments arranged by size: 3041 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2797 3686 5315 Size: 54 1425 50 275 18 967 889 1629 Cuts at: 5315 8 Size: 43 Fragments arranged by size: 1629 1425 967 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2797 1537 Size: 275 985 4090 Fragments arranged by size: 4090 985 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2510 3280 1353 Size: 1157 770 3423 Fragments arranged by size: 3423 1157 770 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3937 4311 4457 387 Size: 1064 138 2348 374 146 1280 Fragments arranged by size: 2348 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 3413 4010 4797 5065 1239 Size: 393 560 1221 597 787 268 1524 Fragments arranged by size: 1524 1221 787 597 560 393 268 EcoO109I rG'GnC_Cy Cuts at: 2131 2131 Size: 5350 EcoRI G'AATT_C Cuts at: 2063 2659 3037 2063 Size: 596 378 4376 Fragments arranged by size: 4376 596 378 FspI TGC'GCA Cuts at: 3856 3856 Size: 5350 HaeII r_GCGC'y Cuts at: 1678 2475 4497 1678 Size: 797 2022 2531 Fragments arranged by size: 2531 2022 797 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 3991 4023 4065 Size: 32 135 32 437 32 1950 32 42 Cuts at: 4065 4097 1373 Size: 32 2626 Fragments arranged by size: 2626 1950 437 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2694 2940 669 Size: 988 281 756 246 3079 Fragments arranged by size: 3079 988 756 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5350 HpaI GTT'AAC Cuts at: 2940 2940 Size: 5350 KasI G'GCGC_C Cuts at: 2471 2471 Size: 5350 KpnI G_GTAC'C Cuts at: 2675 2675 Size: 5350 MfeI C'AATT_G Cuts at: 2949 2949 Size: 5350 MluI A'CGCG_T Cuts at: 5226 5226 Size: 5350 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2277 4464 4648 1305 Size: 972 2187 184 2007 Fragments arranged by size: 2187 2007 972 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3528 Fragments arranged by size: 3528 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2429 2466 3308 3667 3826 518 Size: 648 1263 37 842 359 159 2042 Fragments arranged by size: 2042 1263 842 648 359 159 37 MspA1I CmG'CkG Cuts at: 1606 2599 2766 3443 4591 4836 1606 Size: 993 167 677 1148 245 2120 Fragments arranged by size: 2120 1148 993 677 245 167 NarI GG'CG_CC Cuts at: 2472 2472 Size: 5350 NcoI C'CATG_G Cuts at: 513 1065 2397 513 Size: 552 1332 3466 Fragments arranged by size: 3466 1332 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5350 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5350 NotI GC'GGCC_GC Cuts at: 2797 2797 Size: 5350 NspI r_CATG'y Cuts at: 1132 1229 2503 2708 4253 1132 Size: 97 1274 205 1545 2229 Fragments arranged by size: 2229 1545 1274 205 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5350 PciI A'CATG_T Cuts at: 4249 4249 Size: 5350 PflMI CCAn_nnn'nTGG Cuts at: 1108 2296 2587 1108 Size: 1188 291 3871 Fragments arranged by size: 3871 1188 291 PfoI T'CCnGG_A Cuts at: 2027 2094 2027 Size: 67 5283 PmeI GTTT'AAAC Cuts at: 2654 2654 Size: 5350 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3419 3451 4932 4964 3419 Size: 32 1481 32 3805 Fragments arranged by size: 3805 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2131 2131 Size: 5350 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5350 PsiI TTA'TAA Cuts at: 2920 2920 Size: 5350 PstI C_TGCA'G Cuts at: 830 2604 2702 830 Size: 1774 98 3478 Fragments arranged by size: 3478 1774 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5350 PvuII CAG'CTG Cuts at: 2599 2599 Size: 5350 SalI G'TCGA_C Cuts at: 1936 2692 1936 Size: 756 4594 SapI GCTCTTCn'nnn_ Cuts at: 2510 2510 Size: 5350 SbfI CC_TGCA'GG Cuts at: 2702 2702 Size: 5350 ScaI AGT'ACT Cuts at: 3598 5310 3598 Size: 1712 3638 SfcI C'TryA_G Cuts at: 826 1045 2600 2698 3833 4514 4705 826 Size: 219 1555 98 1135 681 191 1471 Fragments arranged by size: 1555 1471 1135 681 219 191 98 SfoI GGC'GCC Cuts at: 2473 2473 Size: 5350 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5350 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5350 SmaI CCC'GGG Cuts at: 1872 2677 1872 Size: 805 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3454 4355 4617 820 Size: 197 926 1511 901 262 1553 Fragments arranged by size: 1553 1511 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3141 493 Size: 2648 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5350 SphI G_CATG'C Cuts at: 1132 2708 1132 Size: 1576 3774 SspI AAT'ATT Cuts at: 5 52 3274 5 Size: 47 3222 2081 Fragments arranged by size: 3222 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5350 StyI C'CwwG_G Cuts at: 513 1065 2397 2789 513 Size: 552 1332 392 3074 Fragments arranged by size: 3074 1332 552 392 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2455 3377 3394 3547 3732 4177 325 Size: 1255 875 922 17 153 185 445 1498 Fragments arranged by size: 1498 1255 922 875 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2340 2433 3596 Size: 274 80 33 51 1443 363 93 1163 Cuts at: 3596 5308 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1163 363 274 138 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5310 5342 5310 Size: 32 5318 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5350 XbaI T'CTAG_A Cuts at: 2152 2686 2152 Size: 534 4816 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2465 2465 Size: 5350 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5350 XmaI C'CCGG_G Cuts at: 1870 2675 1870 Size: 805 4545 XmnI GAAnn'nnTTC Cuts at: 3479 3479 Size: 5350 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3158 276 Size: 53 83 186 936 1624 2468 Fragments arranged by size: 2468 1624 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI