################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvaI AvaII AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI Ksp632I MaeI MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 262 GTCTAC GT^[AC][GT]AC AlwI 198 GATCC GGATC(4/5) Asp700I 177 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 223 GAGCAC G[AT]GC[AT]^C AspI 256 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 107 GGCGCC GGCGC^C BbvI 66 GCTGC GCAGC(8/12) BcnI 201 CCGGG CC^[GC]GG BglI 119 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 223 GAGCAC G[AGT]GC[ACT]^C BpmI 187 CTGGAG CTGGAG(16/14) BsaI 160 GAGACC GGTCTC(1/5) BseRI 157 GAGGAG GAGGAG(10/8) BsiHKAI 223 GAGCAC G[AT]GC[AT]^C BsiYI 54 CCACCGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 54 CCACCGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 160 GAGAC GTCTC(1/5) BsmFI 203 GGGAC GGGAC(10/14) Bsp1286I 223 GAGCAC G[AGT]GC[ACT]^C BstEII 243 GGTCACC G^GT[ACGT]ACC BstF5I 78 CATCC GGATG(2/0) BstPI 243 GGTCACC G^GT[ACGT]ACC BstXI 251 CCATGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 198 GATC ^GATC DraII 26 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO109I 26 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 243 GGTCACC G^GT[ACGT]ACC EcoT14I 251 CCATGG C^C[AT][AT]GG FauI 164 CCCGC CCCGC(4/6) FokI 78 CATCC GGATG(9/13) HapII 201 CCGG C^CGG HpaII 201 CCGG C^CGG KasI 107 GGCGCC G^GCGCC MboI 198 GATC ^GATC MspI 201 CCGG C^CGG NarI 107 GGCGCC GG^CGCC NciI 201 CCGGG CC^[GC]GG NcoI 251 CCATGG C^CATGG NdeII 198 GATC ^GATC NlaIII 252 CATG CATG^ PflFI 256 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Sau3AI 198 GATC ^GATC SfoI 107 GGCGCC GGC^GCC SmlI 271 CTCAAG C^T[CT][GA]AG StyI 251 CCATGG C^C[AT][AT]GG TseI 66 GCTGC G^C[AT]GC Tth111I 256 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 177 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcyI 107 GGCGCC G[GA]^CG[CT]C 205 GACGCC BanI 43 GGTGCC G^G[CT][GA]CC 107 GGCGCC BsaHI 107 GGCGCC G[GA]^CG[CT]C 205 GACGCC Cfr13I 27 GGGCC G^G[ACGT]CC 282 GGGCC HgaI 8 GACGC GACGC(5/10) 205 GACGC Hin1I 107 GGCGCC G[GA]^CG[CT]C 205 GACGCC HphI 89 TCACC GGTGA(8/7) 245 TCACC KspI 120 CCGCGG CCGC^GG 165 CCGCGG MaeII 194 ACGT A^CGT 257 ACGT MaeIII 211 GTGAC ^GT[ACGT]AC 244 GTCAC MslI 246 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 252 CATGGACGTG MspA1I 120 CCGCGG C[AC]G^C[GT]G 165 CCGCGG NlaIV 43 GGTGCC GG[ACGT]^[ACGT]CC 107 GGCGCC SacII 120 CCGCGG CCGC^GG 165 CCGCGG Sau96I 27 GGGCC G^G[ACGT]CC 282 GGGCC Tsp45I 211 GTGAC ^GT[GC]AC 244 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AccII AciI BsaJI BssKI BstNI BstUI Cac8I CfoI DsaI EcoRII Fnu4HI HaeII HaeIII HhaI HinP1I ItaI MnlI MvaI MvnI MwoI ScrFI