Restriction map for pFN21AE1602
 (Circular) (Six-base) MAPSORT of: pFN21AE1602.seq  Check: 4698  from: 1  to: 5014



pFN21AE1602  5014 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:52  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2824     278 
   Size:         53      83     186     936    1288    2468

  Fragments arranged by size: 

                2468    1288     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2268    2357    1937 
   Size:        331      89    4594

  Fragments arranged by size: 

                4594     331      89
 
Acc65I G'GTAC_C

Cuts at:   2335    2335 
   Size:       5014
 
AclI AA'CG_TT

Cuts at:   1563    3141    3514    1563 
   Size:       1578     373    3063

  Fragments arranged by size: 

                3063    1578     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3077    4461    4729    1632 
   Size:       1445    1384     268    1917

  Fragments arranged by size: 

                1917    1445    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4817
 
AflIII A'CryG_T

Cuts at:   1203    3913    4890    1203 
   Size:       2710     977    1327

  Fragments arranged by size: 

                2710    1327     977
 
AgeI A'CCGG_T

Cuts at:   3845    3845 
   Size:       5014
 
AhdI GACnn_n'nnGTC

Cuts at:   3743    3743 
   Size:       5014
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5014
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2087    1184 
   Size:        903    4111
 
AlwNI CAG_nnn'CTG

Cuts at:   2757    4329    2757 
   Size:       1572    3442
 
ApaLI G'TGCA_C

Cuts at:   3071    4227    3071 
   Size:       1156    3858
 
ApoI r'AATT_y

Cuts at:   1493    2323    2549    2701    4810    1493 
   Size:        830     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     830     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3568     160 
   Size:        872    2536    1606

  Fragments arranged by size: 

                2536    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5014
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2339    1870 
   Size:         73     396    4545

  Fragments arranged by size: 

                4545     396      73
 
BamHI G'GATC_C

Cuts at:   2344    2711    2344 
   Size:        367    4647
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2048    2112    2335    3790     618 
   Size:        325     217     627     261      64     223    1455    1842

  Fragments arranged by size: 

                1842    1455     627     325     261     223     217      64
            
 
BanII G_rGCy'C

Cuts at:   1391    2333    1391 
   Size:        942    4072
 
BbeI G_GCGC'C

Cuts at:   2116    2116 
   Size:       5014
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4043
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3205    3239    1903 
   Size:         34    1268      34    3678

  Fragments arranged by size: 

                3678    1268      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2906    4116    2906 
   Size:       1210    3804
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5014
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2130    3625     136 
   Size:        107     122      71     631     605     458    1495    1525

  Fragments arranged by size: 

                1525    1495     631     605     458     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5009    5009 
   Size:       5014
 
BlpI GC'TnA_GC

Cuts at:   2431    2431 
   Size:       5014
 
Bme1580I G_kGCm'C

Cuts at:   1790    3075    4231    1790 
   Size:       1285    1156    2573

  Fragments arranged by size: 

                2573    1285    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2263    1318 
   Size:        945    4069
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2745    3703     442 
   Size:        416      53      83     399      24    1328     958    1753

  Fragments arranged by size: 

                1753    1328     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5014
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2213    3674    1239 
   Size:        974    1461    2579

  Fragments arranged by size: 

                2579    1461     974
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2261    3139    4004    4302    2261 
   Size:        878     865     298    2973

  Fragments arranged by size: 

                2973     878     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2159    3677     882 
   Size:        617     660    1518    2219

  Fragments arranged by size: 

                2219    1518     660     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2805     493 
   Size:        711    1601    2702

  Fragments arranged by size: 

                2702    1601     711
 
BsaBI GATnn'nnATC

Cuts at:   5008    5008 
   Size:       5014
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2113    2211    2821    3203 
   Size:         53      83     186     936     580      98     610     382

Cuts at:   3203    3838     275 
   Size:        635    1451

  Fragments arranged by size: 

                1451     936     635     610     580     382     186      98
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   3447    3845    4119    4266    4677    3447 
   Size:        398     274     147     411    3784

  Fragments arranged by size: 

                3784     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4789

  Fragments arranged by size: 

                4789     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2177    1186 
   Size:        991    4023
 
BseYI C'CCAG_C

Cuts at:   4217    4713    4217 
   Size:        496    4518
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4441
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2464    3225    4253    1540 
   Size:        275     186     463     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     463     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2232    3075    3160    4231    2232 
   Size:        843      85    1071    3015

  Fragments arranged by size: 

                3015    1071     843      85
 
BsmI GAATG_Cn'

Cuts at:   2525    2618    2525 
   Size:         93    4921
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2232    2333    3075    3160    4231    1391 
   Size:        399     442     101     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     442     399     101      85
 
BspEI T'CCGG_A

Cuts at:   4677    4677 
   Size:       5014
 
BspHI T'CATG_A

Cuts at:   2903    4633    2903 
   Size:       1730    3284
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2369     844 
   Size:       1525    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2901    1394 
   Size:        429    1078    3507

  Fragments arranged by size: 

                3507    1078     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3509    3683      65 
   Size:       1183    2261     174    1396

  Fragments arranged by size: 

                2261    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3658    3845    1700 
   Size:        110    1848     187    2869

  Fragments arranged by size: 

                2869    1848     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5014
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5014
 
BssSI C'ACGA_G

Cuts at:   3074    4086    3074 
   Size:       1012    4002
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5014
 
BstBI TT'CG_AA

Cuts at:   3818    3818 
   Size:       5014
 
BstEII G'GTnAC_C

Cuts at:   1184    2248    1184 
   Size:       1064    3950
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2263    2263 
   Size:       5014
 
BstYI r'GATC_y

Cuts at:   1972    2344    2711    3096    3113    4565    4651    4663    5009 
   Size:        372     367     385      17    1452      86      12     346

Cuts at:   5009    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     385     372     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2125    2170    2256     513 
   Size:        552    1060      45      86    3271

  Fragments arranged by size: 

                3271    1060     552      86      45
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4275

  Fragments arranged by size: 

                4275     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2530    3324    3344    2530 
   Size:        794      20    4200

  Fragments arranged by size: 

                4200     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5014
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    4979
 
DraI TTT'AAA

Cuts at:   2318    2318 
   Size:       5014
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4021     809 
   Size:        507    2705    1802

  Fragments arranged by size: 

                2705    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2461    3350    4979 
   Size:         54    1425      50     275      18     631     889    1629

Cuts at:   4979       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     631     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2461    1537 
   Size:        275     649    4090

  Fragments arranged by size: 

                4090     649     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2944    1353 
   Size:       1591    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3601    3975    4121     387 
   Size:       1064     138    2012     374     146    1280

  Fragments arranged by size: 

                2012    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2213    3077    3674    4461    4729    1239 
   Size:        393     581     864     597     787     268    1524

  Fragments arranged by size: 

                1524     864     787     597     581     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2032    2032 
   Size:       5014
 
EcoRI G'AATT_C

Cuts at:   2323    2701    2323 
   Size:        378    4636
 
FspI TGC'GCA

Cuts at:   3520    3520 
   Size:       5014
 
HaeII r_GCGC'y

Cuts at:   1678    2059    2116    2284    4161    1678 
   Size:        381      57     168    1877    2531

  Fragments arranged by size: 

                2531    1877     381     168      57
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3655    3687    3729    3761    1373 
   Size:         32     135      32    2083      32      42      32    2626

  Fragments arranged by size: 

                2626    2083     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2358    2604     669 
   Size:        988     281     420     246    3079

  Fragments arranged by size: 

                3079     988     420     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5014
 
HpaI GTT'AAC

Cuts at:   2604    2604 
   Size:       5014
 
KasI G'GCGC_C

Cuts at:   2112    2112 
   Size:       5014
 
KpnI G_GTAC'C

Cuts at:   2339    2339 
   Size:       5014
 
MfeI C'AATT_G

Cuts at:   2613    2613 
   Size:       5014
 
MluI A'CGCG_T

Cuts at:   4890    4890 
   Size:       5014
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4128    4312    1305 
   Size:       2823     184    2007

  Fragments arranged by size: 

                2823    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3192

  Fragments arranged by size: 

                3192    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2255    2261    2972    3331    3490     518 
   Size:        648    1089       6     711     359     159    2042

  Fragments arranged by size: 

                2042    1089     711     648     359     159       6
 
MspA1I CmG'CkG

Cuts at:   1606    2127    2172    2430    3107    4255    4500    1606 
   Size:        521      45     258     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     521     258     245      45
 
NarI GG'CG_CC

Cuts at:   2113    2113 
   Size:       5014
 
NcoI C'CATG_G

Cuts at:    513    1065    2256     513 
   Size:        552    1191    3271

  Fragments arranged by size: 

                3271    1191     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5014
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5014
 
NotI GC'GGCC_GC

Cuts at:   2461    2461 
   Size:       5014
 
NspI r_CATG'y

Cuts at:   1132    1229    2372    3917    1132 
   Size:         97    1143    1545    2229

  Fragments arranged by size: 

                2229    1545    1143      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5014
 
PciI A'CATG_T

Cuts at:   3913    3913 
   Size:       5014
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5014
 
PfoI T'CCnGG_A

Cuts at:   2190    2205    2190 
   Size:         15    4999
 
PmeI GTTT'AAAC

Cuts at:   2318    2318 
   Size:       5014
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3083    3115    4596    4628    3083 
   Size:         32    1481      32    3469

  Fragments arranged by size: 

                3469    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5014
 
PsiI TTA'TAA

Cuts at:   2584    2584 
   Size:       5014
 
PstI C_TGCA'G

Cuts at:    830    2366     830 
   Size:       1536    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5014
 
SacII CC_GC'GG

Cuts at:   2128    2173    2128 
   Size:         45    4969
 
SalI G'TCGA_C

Cuts at:   1936    2356    1936 
   Size:        420    4594
 
SbfI CC_TGCA'GG

Cuts at:   2366    2366 
   Size:       5014
 
ScaI AGT'ACT

Cuts at:   3262    4974    3262 
   Size:       1712    3302
 
SfcI C'TryA_G

Cuts at:    826    1045    2362    3497    4178    4369     826 
   Size:        219    1317    1135     681     191    1471

  Fragments arranged by size: 

                1471    1317    1135     681     219     191
 
SfoI GGC'GCC

Cuts at:   2114    2114 
   Size:       5014
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5014
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5014
 
SmaI CCC'GGG

Cuts at:   1872    2341    1872 
   Size:        469    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2276    3118    4019    4281     820 
   Size:        197     926     333     842     901     262    1553

  Fragments arranged by size: 

                1553     926     901     842     333     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2805     493 
   Size:       2312    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5014
 
SphI G_CATG'C

Cuts at:   1132    2372    1132 
   Size:       1240    3774
 
SspI AAT'ATT

Cuts at:      5      52    2938       5 
   Size:         47    2886    2081

  Fragments arranged by size: 

                2886    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5014
 
StyI C'CwwG_G

Cuts at:    513    1065    2256    2453     513 
   Size:        552    1191     197    3074

  Fragments arranged by size: 

                3074    1191     552     197
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3041    3058    3211    3396    3841     325 
   Size:       1255    1461      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1461    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3260    4972      96 
   Size:        274      80      33      51    1443    1283    1712     138

  Fragments arranged by size: 

                1712    1443    1283     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2032    2064    4974    5006    2032 
   Size:         32    2910      32    2040

  Fragments arranged by size: 

                2910    2040      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2264    1316 
   Size:        948    4066
 
XbaI T'CTAG_A

Cuts at:   2350    2350 
   Size:       5014
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5014
 
XmaI C'CCGG_G

Cuts at:   1870    2339    1870 
   Size:        469    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2186    3143    2186 
   Size:        957    4057
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2822     276 
   Size:         53      83     186     936    1288    2468

  Fragments arranged by size: 

                2468    1288     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI    SacII     SalI     SbfI     ScaI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     NaeI   NgoMIV
     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    PvuII    RsrII     SacI    SanDI     SapI    SexAI     SfiI     SrfI
     SwaI     XcmI
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