################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrDI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DrdI Eam1105I EarI Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FokI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MroI MseI MspA1I MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 189 GAATTC [GA]^AATT[CT] AluI 109 AGCT AG^CT AlwI 282 GATCC GGATC(4/5) ApoI 189 GAATTC [GA]^AATT[CT] AvaII 144 GGTCC G^G[AT]CC BalI 320 TGGCCA TGG^CCA BanII 39 GGGCTC G[GA]GC[CT]^C BmyI 39 GGGCTC G[AGT]GC[ACT]^C BpmI 273 CTCCAG CTGGAG(16/14) BsiEI 290 CGGCCG CG[GA][CT]^CG Bsp1286I 39 GGGCTC G[AGT]GC[ACT]^C Bsp1407I 125 TGTACA T^GTACA BspHI 185 TCATGA T^CATGA BsrFI 157 ACCGGC [GA]^CCGG[CT] BsrGI 125 TGTACA T^GTACA BstXI 170 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 281 AGATCC [GA]^GATC[CT] Cfr10I 157 ACCGGC [GA]^CCGG[CT] Cfr13I 144 GGTCC G^G[ACGT]CC DpnII 282 GATC ^GATC DraIII 328 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 176 CCATGG C^C[GA][CT]GG EagI 290 CGGCCG C^GGCCG EclXI 290 CGGCCG C^GGCCG Eco52I 290 CGGCCG C^GGCCG EcoRI 189 GAATTC G^AATTC HapII 158 CCGG C^CGG HpaII 158 CCGG C^CGG MboI 282 GATC ^GATC MboII 69 GAAGA GAAGA(8/7) MflI 281 AGATCC [GA]^GATC[CT] MluNI 320 TGGCCA TGG^CCA MscI 320 TGGCCA TGG^CCA MslI 117 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 158 CCGG C^CGG NcoI 176 CCATGG C^CATGG NdeII 282 GATC ^GATC NlaIV 40 GGCTCC GG[ACGT]^[ACGT]CC RcaI 185 TCATGA T^CATGA Sau3AI 282 GATC ^GATC Sau96I 144 GGTCC G^G[ACGT]CC SspBI 125 TGTACA T^GTACA Tsp509I 190 AATT ^AATT XhoII 281 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### Aor51HI 257 AGCGCT AGC^GCT 370 AGCGCT BspMI 141 GCAGGT ACCTGC(4/8) 223 GCAGGT EaeI 290 CGGCCG [CT]^GGCC[GA] 320 TGGCCA Eco47III 257 AGCGCT AGC^GCT 370 AGCGCT EcoT14I 170 CCAAGG C^C[AT][AT]GG 176 CCATGG FauI 25 CCCGC CCCGC(4/6) 236 CCCGC HaeII 257 AGCGCT [GA]GCGC^[CT] 370 AGCGCT MaeIII 55 GTGAC ^GT[ACGT]AC 355 GTCAC NlaIII 177 CATG CATG^ 186 CATG SfaNI 182 GCATC GCATC(5/9) 218 GCATC StyI 170 CCAAGG C^C[AT][AT]GG 176 CCATGG TaqI 212 TCGA T^CGA 262 TCGA Tsp45I 55 GTGAC ^GT[GC]AC 355 GTCAC TthHB8I 212 TCGA T^CGA 262 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AfaI BbvI BsaJI BsiYI BslI BssKI BstNI Cac8I CfoI DdeI EcoRII Fnu4HI HaeIII HhaI HinP1I HphI ItaI MnlI MvaI MwoI RsaI ScrFI TseI