################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I MaeI MaeII MamI MfeI MseI MslI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 171 GTCTAC GT^[AC][GT]AC AccIII 188 TCCGGA T^CCGGA AfaI 8 GTAC GT^AC AflIII 348 ACGCGT A^C[GA][CT]GT AlwI 153 GATCC GGATC(4/5) AlwNI 205 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 85 AGCGCT AGC^GCT BalI 62 TGGCCA TGG^CCA BanI 133 GGCACC G^G[CT][GA]CC BcgI 15 GCAGACGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaWI 188 TCCGGA [AT]^CCGG[AT] BseAI 188 TCCGGA T^CCGGA BsiYI 91 CCGGCCACGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 91 CCGGCCACGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 389 GCATTC GAATGC(1/-1) BspEI 188 TCCGGA T^CCGGA BspMI 245 ACCTGC ACCTGC(4/8) BstF5I 372 CATCC GGATG(2/0) BstYI 152 AGATCC [GA]^GATC[CT] DpnII 153 GATC ^GATC Eco47III 85 AGCGCT AGC^GCT FauI 383 CCCGC CCCGC(4/6) FokI 372 CATCC GGATG(9/13) HphI 267 GGTGA GGTGA(8/7) KspI 263 CCGCGG CCGC^GG MaeIII 352 GTGAC ^GT[ACGT]AC MboI 153 GATC ^GATC MboII 108 GAAGA GAAGA(8/7) MflI 152 AGATCC [GA]^GATC[CT] MluI 348 ACGCGT A^CGCGT MluNI 62 TGGCCA TGG^CCA MroI 188 TCCGGA T^CCGGA MscI 62 TGGCCA TGG^CCA NdeII 153 GATC ^GATC NlaIV 133 GGCACC GG[ACGT]^[ACGT]CC PstI 277 CTGCAG CTGCA^G PvuII 58 CAGCTG CAG^CTG RsaI 8 GTAC GT^AC SacII 263 CCGCGG CCGC^GG Sau3AI 153 GATC ^GATC SfcI 277 CTGCAG C^T[GA][CT]AG SfiI 285 GGCCTGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TaqI 314 TCGA T^CGA Tsp45I 352 GTGAC ^GT[GC]AC TthHB8I 314 TCGA T^CGA XhoII 152 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BglI 64 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 286 GCCTGCGAGGC BssKI 130 CCTGG ^CC[ACGT]GG 210 CCTGG BstNI 130 CCTGG CC^[AT]GG 210 CCTGG EaeI 62 TGGCCA [CT]^GGCC[GA] 92 CGGCCA EcoRII 130 CCTGG ^CC[AT]GG 210 CCTGG HapII 91 CCGG C^CGG 189 CCGG HpaII 91 CCGG C^CGG 189 CCGG MspA1I 58 CAGCTG C[AC]G^C[GT]G 263 CCGCGG MspI 91 CCGG C^CGG 189 CCGG MvaI 130 CCTGG CC^[AT]GG 210 CCTGG NlaIII 340 CATG CATG^ 360 CATG ScrFI 130 CCTGG CC^[ACGT]GG 210 CCTGG StuI 284 AGGCCT AGG^CCT 293 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AccII AciI AluI BbvI BsaJI BstUI Cac8I CfoI DdeI DsaI Fnu4HI HaeII HaeIII HhaI HinP1I ItaI MnlI MvnI MwoI TseI