Restriction map for
pFN21AE1214
(Circular) (Six-base) MAPSORT of: pFN21AE1214.seq Check: 6337 from: 1 to: 5113
pFN21AE1214 5113 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2923 278
Size: 53 83 186 936 1387 2468
Fragments arranged by size:
2468 1387 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2177 2456 1937
Size: 240 279 4594
Fragments arranged by size:
4594 279 240
Acc65I G'GTAC_C
Cuts at: 2434 2434
Size: 5113
AclI AA'CG_TT
Cuts at: 1563 3240 3613 1563
Size: 1677 373 3063
Fragments arranged by size:
3063 1677 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3176 4560 4828 1632
Size: 1544 1384 268 1917
Fragments arranged by size:
1917 1544 1384 268
AfeI AGC'GCT
Cuts at: 2092 2092
Size: 5113
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4916
AflIII A'CryG_T
Cuts at: 1203 2353 4012 4989 1203
Size: 1150 1659 977 1327
Fragments arranged by size:
1659 1327 1150 977
AgeI A'CCGG_T
Cuts at: 3944 3944
Size: 5113
AhdI GACnn_n'nnGTC
Cuts at: 3842 3842
Size: 5113
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5113
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5113
AlwNI CAG_nnn'CTG
Cuts at: 2215 2856 4428 2215
Size: 641 1572 2900
Fragments arranged by size:
2900 1572 641
ApaLI G'TGCA_C
Cuts at: 3170 4326 3170
Size: 1156 3957
ApoI r'AATT_y
Cuts at: 1493 2422 2648 2800 4909 1493
Size: 929 226 152 2109 1697
Fragments arranged by size:
2109 1697 929 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3667 160
Size: 872 2635 1606
Fragments arranged by size:
2635 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5113
AvaI C'yCGr_G
Cuts at: 1870 1943 2438 1870
Size: 73 495 4545
Fragments arranged by size:
4545 495 73
BamHI G'GATC_C
Cuts at: 2443 2810 2443
Size: 367 4746
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2434 3889 618
Size: 325 217 627 351 296 1455 1842
Fragments arranged by size:
1842 1455 627 351 325 296 217
BanII G_rGCy'C
Cuts at: 1391 2432 1391
Size: 1041 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2009 2043 3304 3338 1903
Size: 34 72 34 1261 34 3678
Fragments arranged by size:
3678 1261 72 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3005 4215 3005
Size: 1210 3903
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5113
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2075 2297 3724
Size: 107 122 71 631 605 403 222 1427
Cuts at: 3724 136
Size: 1525
Fragments arranged by size:
1525 1427 631 605 403 222 122 107
71
BglII A'GATC_T
Cuts at: 5108 5108
Size: 5113
BlpI GC'TnA_GC
Cuts at: 2530 2530
Size: 5113
Bme1580I G_kGCm'C
Cuts at: 1790 3174 4330 1790
Size: 1384 1156 2573
Fragments arranged by size:
2573 1384 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5113
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2844 3802 442
Size: 416 53 83 399 24 1427 958 1753
Fragments arranged by size:
1753 1427 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3773 1239
Size: 2534 2579
Bpu10I CC'TnA_GC
Cuts at: 2232 2232
Size: 5113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3238 4103 4401 3238
Size: 865 298 3950
Fragments arranged by size:
3950 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3776 882
Size: 617 2277 2219
Fragments arranged by size:
2277 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2904 493
Size: 711 1700 2702
Fragments arranged by size:
2702 1700 711
BsaBI GATnn'nnATC
Cuts at: 5107 5107
Size: 5113
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2920 3302 3937 275
Size: 53 83 186 936 1387 382 635 1451
Fragments arranged by size:
1451 1387 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2193 3546 3944 4218 4365 4776 2193
Size: 1353 398 274 147 411 2530
Fragments arranged by size:
2530 1353 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4888
Fragments arranged by size:
4888 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5113
BseYI C'CCAG_C
Cuts at: 4316 4812 4316
Size: 496 4617
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4540
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2563 3324 4352 1540
Size: 275 186 562 761 1028 2301
Fragments arranged by size:
2301 1028 761 562 275 186
BsiHKAI G_wGCw'C
Cuts at: 3174 3259 4330 3174
Size: 85 1071 3957
Fragments arranged by size:
3957 1071 85
BsmI GAATG_Cn'
Cuts at: 2394 2624 2717 2394
Size: 230 93 4790
Fragments arranged by size:
4790 230 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2432 3174 3259 4330 1391
Size: 399 642 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 642 399 85
BspEI T'CCGG_A
Cuts at: 2193 4776 2193
Size: 2583 2530
BspHI T'CATG_A
Cuts at: 3002 4732 3002
Size: 1730 3383
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2259 2468 844
Size: 1415 209 3489
Fragments arranged by size:
3489 1415 209
BsrBI CCG'CTC
Cuts at: 1394 1823 3000 1394
Size: 429 1177 3507
Fragments arranged by size:
3507 1177 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3608 3782 65
Size: 1183 2360 174 1396
Fragments arranged by size:
2360 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3757 3944 1700
Size: 110 1947 187 2869
Fragments arranged by size:
2869 1947 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5113
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5113
BssSI C'ACGA_G
Cuts at: 3173 4185 3173
Size: 1012 4101
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5113
BstBI TT'CG_AA
Cuts at: 3917 3917
Size: 5113
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5113
BstYI r'GATC_y
Cuts at: 1972 2157 2443 2810 3195 3212 4664 4750 4762
Size: 185 286 367 385 17 1452 86 12
Cuts at: 4762 5108 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 286 185 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2100 2142 2268 513
Size: 552 939 96 42 126 3358
Fragments arranged by size:
3358 939 552 126 96 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2108 487
Size: 683 56 882 3492
Fragments arranged by size:
3492 882 683 56
BtsI GCAGTG_nn'
Cuts at: 2629 3423 3443 2629
Size: 794 20 4299
Fragments arranged by size:
4299 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5113
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5078
DraI TTT'AAA
Cuts at: 2417 2417
Size: 5113
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4120 809
Size: 507 2804 1802
Fragments arranged by size:
2804 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2097 2560
Size: 54 1425 50 275 18 237 30 463
Cuts at: 2560 3449 5078 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 463 275 237 54 50
43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2560 1537
Size: 275 748 4090
Fragments arranged by size:
4090 748 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3043 1353
Size: 1690 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3700 4074 4220 387
Size: 1064 138 2111 374 146 1280
Fragments arranged by size:
2111 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3176 3773 4560 4828 1239
Size: 393 1544 597 787 268 1524
Fragments arranged by size:
1544 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2422 2800 2422
Size: 378 4735
FspI TGC'GCA
Cuts at: 3619 3619
Size: 5113
HaeII r_GCGC'y
Cuts at: 1678 2094 2215 2282 4260 1678
Size: 416 121 67 1978 2531
Fragments arranged by size:
2531 1978 416 121 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3754 3786 3828 3860 1373
Size: 32 135 32 2182 32 42 32 2626
Fragments arranged by size:
2626 2182 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2457 2703 669
Size: 988 281 519 246 3079
Fragments arranged by size:
3079 988 519 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5113
HpaI GTT'AAC
Cuts at: 2703 2703
Size: 5113
KpnI G_GTAC'C
Cuts at: 2438 2438
Size: 5113
MfeI C'AATT_G
Cuts at: 2712 2712
Size: 5113
MluI A'CGCG_T
Cuts at: 2353 4989 2353
Size: 2636 2477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4227 4411 1305
Size: 2922 184 2007
Fragments arranged by size:
2922 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2069 10
Size: 54 1425 343 237 3054
Fragments arranged by size:
3054 1425 343 237 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3071 3430 3589 518
Size: 648 1905 359 159 2042
Fragments arranged by size:
2042 1905 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2065 2270 2529 3206 4354 4599 1606
Size: 459 205 259 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 459 259 245 205
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3622
Fragments arranged by size:
3622 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5113
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5113
NotI GC'GGCC_GC
Cuts at: 2560 2560
Size: 5113
NspI r_CATG'y
Cuts at: 1132 1229 2471 4016 1132
Size: 97 1242 1545 2229
Fragments arranged by size:
2229 1545 1242 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5113
PciI A'CATG_T
Cuts at: 4012 4012
Size: 5113
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5113
PmeI GTTT'AAAC
Cuts at: 2417 2417
Size: 5113
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3182 3214 4695 4727 3182
Size: 32 1481 32 3568
Fragments arranged by size:
3568 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5113
PsiI TTA'TAA
Cuts at: 2683 2683
Size: 5113
PstI C_TGCA'G
Cuts at: 830 2286 2465 830
Size: 1456 179 3478
Fragments arranged by size:
3478 1456 179
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5113
PvuII CAG'CTG
Cuts at: 2065 2065
Size: 5113
SacII CC_GC'GG
Cuts at: 2271 2271
Size: 5113
SalI G'TCGA_C
Cuts at: 1936 2455 1936
Size: 519 4594
SbfI CC_TGCA'GG
Cuts at: 2465 2465
Size: 5113
ScaI AGT'ACT
Cuts at: 3361 5073 3361
Size: 1712 3401
SfcI C'TryA_G
Cuts at: 826 1045 2282 2461 3596 4277 4468 826
Size: 219 1237 179 1135 681 191 1471
Fragments arranged by size:
1471 1237 1135 681 219 191 179
SfiI GGCCn_nnn'nGGCC
Cuts at: 2297 2297
Size: 5113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5113
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5113
SmaI CCC'GGG
Cuts at: 1872 2440 1872
Size: 568 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3217 4118 4380 820
Size: 197 926 1274 901 262 1553
Fragments arranged by size:
1553 1274 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2904 493
Size: 2411 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5113
SphI G_CATG'C
Cuts at: 1132 2471 1132
Size: 1339 3774
SspI AAT'ATT
Cuts at: 5 52 3037 5
Size: 47 2985 2081
Fragments arranged by size:
2985 2081 47
StuI AGG'CCT
Cuts at: 1517 2291 2300 1517
Size: 774 9 4330
Fragments arranged by size:
4330 774 9
StyI C'CwwG_G
Cuts at: 513 1065 2004 2552 513
Size: 552 939 548 3074
Fragments arranged by size:
3074 939 552 548
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2261 3140 3157 3310 3495 3940 325
Size: 1255 681 879 17 153 185 445 1498
Fragments arranged by size:
1498 1255 879 681 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3359 5071 96
Size: 274 80 33 51 1443 1382 1712 138
Fragments arranged by size:
1712 1443 1382 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5073 5105 5073
Size: 32 5081
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5113
XbaI T'CTAG_A
Cuts at: 2449 2449
Size: 5113
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5113
XmaI C'CCGG_G
Cuts at: 1870 2438 1870
Size: 568 4545
XmnI GAAnn'nnTTC
Cuts at: 3242 3242
Size: 5113
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2921 276
Size: 53 83 186 936 1387 2468
Fragments arranged by size:
2468 1387 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfoI SrfI SwaI XcmI