################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HpaI HphI KasI KpnI Ksp632I KspI MamI MfeI MluNI MroI MscI MseI MslI MunI NarI NciI NcoI NdeI NlaIII NotI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AfaI 150 GTAC GT^AC AflIII 49 ACGCGT A^C[GA][CT]GT Alw44I 91 GTGCAC G^TGCAC ApaLI 91 GTGCAC G^TGCAC AspHI 91 GTGCAC G[AT]GC[AT]^C AvaI 32 CTCGGG C^[CT]CG[GA]G BglII 185 AGATCT A^GATCT BmyI 91 GTGCAC G[AGT]GC[ACT]^C BpmI 143 CTCCAG CTGGAG(16/14) BsiEI 179 CGGCCG CG[GA][CT]^CG BsiHKAI 91 GTGCAC G[AT]GC[AT]^C BsoBI 32 CTCGGG C^[CT]CG[GA]G Bsp1286I 91 GTGCAC G[AGT]GC[ACT]^C BspMI 248 ACCTGC ACCTGC(4/8) BstYI 185 AGATCT [GA]^GATC[CT] DpnII 186 GATC ^GATC EagI 179 CGGCCG C^GGCCG EclXI 179 CGGCCG C^GGCCG Eco52I 179 CGGCCG C^GGCCG EcoT14I 380 CCAAGG C^C[AT][AT]GG HaeII 139 GGCGCT [GA]GCGC^[CT] HindIII 286 AAGCTT A^AGCTT HinfI 128 GAGTC G^A[ACGT]TC MaeII 9 ACGT A^CGT MaeIII 301 GTGAC ^GT[ACGT]AC MboI 186 GATC ^GATC MflI 185 AGATCT [GA]^GATC[CT] MluI 49 ACGCGT A^CGCGT MspA1I 158 CAGCGG C[AC]G^C[GT]G NaeI 136 GCCGGC GCC^GGC NdeII 186 GATC ^GATC NgoMIV 136 GCCGGC G^CCGGC NlaIV 133 GGGGCC GG[ACGT]^[ACGT]CC NruI 213 TCGCGA TCG^CGA PleI 128 GAGTC GAGTC(4/5) PstI 250 CTGCAG CTGCA^G RsaI 150 GTAC GT^AC Sau3AI 186 GATC ^GATC SfaNI 233 GCATC GCATC(5/9) SfcI 250 CTGCAG C^T[GA][CT]AG StyI 380 CCAAGG C^C[AT][AT]GG Tsp45I 301 GTGAC ^GT[GC]AC TspRI 146 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 185 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BsaJI 59 CCAGGG C^C[ACGT][ACGT]GG 380 CCAAGG BseRI 100 CTCCTC GAGGAG(10/8) 274 GAGGAG BsiYI 153 CCTGGCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 369 CCACCATAAGG BslI 153 CCTGGCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 369 CCACCATAAGG BsrFI 64 GCCGGT [GA]^CCGG[CT] 136 GCCGGC BsrI 79 CCAGT ACTGG(1/-1) 145 CCAGT Cfr10I 64 GCCGGT [GA]^CCGG[CT] 136 GCCGGC Cfr13I 62 GGGCC G^G[ACGT]CC 134 GGGCC DdeI 189 CTTAG C^T[ACGT]AG 353 CTAAG EaeI 84 TGGCCG [CT]^GGCC[GA] 179 CGGCCG FauI 230 CCCGC CCCGC(4/6) 240 CCCGC HapII 65 CCGG C^CGG 137 CCGG HpaII 65 CCGG C^CGG 137 CCGG MspI 65 CCGG C^CGG 137 CCGG NheI 195 GCTAGC G^CTAGC 255 GCTAGC Sau96I 62 GGGCC G^G[ACGT]CC 134 GGGCC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AccII AciI AluI BbsI BbvI BfaI BpuAI BssKI BstF5I BstNI BstUI Cac8I CfoI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I ItaI MaeI MboII MnlI MvaI MvnI MwoI ScrFI TseI