Restriction map for pFN21AE6155
 (Circular) (Six-base) MAPSORT of: pFN21AE6155.seq  Check: 496  from: 1  to: 5095



pFN21AE6155  5095 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:53  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2262    2262 
   Size:       5095
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2905     278 
   Size:         53      83     186     936    1369    2468

  Fragments arranged by size: 

                2468    1369     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2438    1937 
   Size:        501    4594
 
Acc65I G'GTAC_C

Cuts at:   2416    2416 
   Size:       5095
 
AclI AA'CG_TT

Cuts at:   1563    3222    3595    1563 
   Size:       1659     373    3063

  Fragments arranged by size: 

                3063    1659     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2060    3158    4542    4810    1632 
   Size:        428    1098    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1098     428     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4898
 
AflIII A'CryG_T

Cuts at:   1203    2054    3994    4971    1203 
   Size:        851    1940     977    1327

  Fragments arranged by size: 

                1940    1327     977     851
 
AgeI A'CCGG_T

Cuts at:   3926    3926 
   Size:       5095
 
AhdI GACnn_n'nnGTC

Cuts at:   3824    3824 
   Size:       5095
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5095
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5095
 
AlwNI CAG_nnn'CTG

Cuts at:   2838    4410    2838 
   Size:       1572    3523
 
ApaLI G'TGCA_C

Cuts at:   2096    3152    4308    2096 
   Size:       1056    1156    2883

  Fragments arranged by size: 

                2883    1156    1056
 
ApoI r'AATT_y

Cuts at:   1493    2404    2630    2782    4891    1493 
   Size:        911     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     911     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3649     160 
   Size:        872    2617    1606

  Fragments arranged by size: 

                2617    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5095
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2037    2420    1870 
   Size:         73      94     383    4545

  Fragments arranged by size: 

                4545     383      94      73
 
BamHI G'GATC_C

Cuts at:   2425    2792    2425 
   Size:        367    4728
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2416    3871     618 
   Size:        325     217     627     629    1455    1842

  Fragments arranged by size: 

                1842    1455     629     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2414    1391 
   Size:       1023    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2066    2237    2369     928 
   Size:        971     167     171     132    3654

  Fragments arranged by size: 

                3654     971     171     167     132
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3286    3320    1903 
   Size:         34    1349      34    3678

  Fragments arranged by size: 

                3678    1349      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2987    4197    2987 
   Size:       1210    3885
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5095
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3706     136 
   Size:        107     122      71     631     605    2034    1525

  Fragments arranged by size: 

                2034    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2190    5090    2190 
   Size:       2900    2195
 
BlpI GC'TnA_GC

Cuts at:   2512    2512 
   Size:       5095
 
Bme1580I G_kGCm'C

Cuts at:   1790    2100    3156    4312    1790 
   Size:        310    1056    1156    2573

  Fragments arranged by size: 

                2573    1156    1056     310
 
BmgBI CAC'GTC

Cuts at:   1318    2015    1318 
   Size:        697    4398
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2826    3784     442 
   Size:        416      53      83     399      24    1409     958    1753

  Fragments arranged by size: 

                1753    1409     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    2204    2264    1056 
   Size:       1148      60    3887

  Fragments arranged by size: 

                3887    1148      60
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2133    3755    1239 
   Size:        894    1622    2579

  Fragments arranged by size: 

                2579    1622     894
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3220    4085    4383    3220 
   Size:        865     298    3932

  Fragments arranged by size: 

                3932     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3758     882 
   Size:        617    2259    2219

  Fragments arranged by size: 

                2259    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2886     493 
   Size:        711    1682    2702

  Fragments arranged by size: 

                2702    1682     711
 
BsaBI GATnn'nnATC

Cuts at:   5089    5089 
   Size:       5095
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2902    3284    3919     275 
   Size:         53      83     186     936    1369     382     635    1451

  Fragments arranged by size: 

                1451    1369     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3528    3926    4200    4347    4758    3528 
   Size:        398     274     147     411    3865

  Fragments arranged by size: 

                3865     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4870

  Fragments arranged by size: 

                4870     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2096    2294    1186 
   Size:        910     198    3987

  Fragments arranged by size: 

                3987     910     198
 
BseYI C'CCAG_C

Cuts at:   4298    4794    4298 
   Size:        496    4599
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4522
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2187    2545    3306    4334    1540 
   Size:        275     186     186     358     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     358     275     186     186
 
BsiHKAI G_wGCw'C

Cuts at:   2100    3156    3241    4312    2100 
   Size:       1056      85    1071    2883

  Fragments arranged by size: 

                2883    1071    1056      85
 
BsmI GAATG_Cn'

Cuts at:   2606    2699    2606 
   Size:         93    5002
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2100    2414    3156    3241    4312    1391 
   Size:        399     310     314     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     314     310      85
 
BspEI T'CCGG_A

Cuts at:   4758    4758 
   Size:       5095
 
BspHI T'CATG_A

Cuts at:   2984    4714    2984 
   Size:       1730    3365
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2262    2450     844 
   Size:       1418     188    3489

  Fragments arranged by size: 

                3489    1418     188
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2982    1394 
   Size:        429    1159    3507

  Fragments arranged by size: 

                3507    1159     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3590    3764      65 
   Size:       1183    2342     174    1396

  Fragments arranged by size: 

                2342    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2069    2141    3739    3926    1700 
   Size:        110     259      72    1598     187    2869

  Fragments arranged by size: 

                2869    1598     259     187     110      72
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5095
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5095
 
BssSI C'ACGA_G

Cuts at:   3155    4167    3155 
   Size:       1012    4083
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5095
 
BstBI TT'CG_AA

Cuts at:   3899    3899 
   Size:       5095
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5095
 
BstYI r'GATC_y

Cuts at:   1972    2190    2425    2792    3177    3194    4646    4732    4744 
   Size:        218     235     367     385      17    1452      86      12

Cuts at:   4744    5090    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     385     367     346     235     218      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4543
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2296     487 
   Size:        683      56    1070    3286

  Fragments arranged by size: 

                3286    1070     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2611    3405    3425    2611 
   Size:        794      20    4281

  Fragments arranged by size: 

                4281     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5095
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5060
 
DraI TTT'AAA

Cuts at:   2399    2399 
   Size:       5095
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4102     809 
   Size:        507    2786    1802

  Fragments arranged by size: 

                2786    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2089    2184    2542 
   Size:         54    1425      50     275      18     259      95     358

Cuts at:   2542    3431    5060       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     358     275     259      95      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2184    2542    1537 
   Size:        275     372     358    4090

  Fragments arranged by size: 

                4090     372     358     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3025    1353 
   Size:       1672    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3682    4056    4202     387 
   Size:       1064     138    2093     374     146    1280

  Fragments arranged by size: 

                2093    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2060    2133    3158    3755    4542    4810    1239 
   Size:        393     428      73    1025     597     787     268    1524

  Fragments arranged by size: 

                1524    1025     787     597     428     393     268      73
            
 
EcoRI G'AATT_C

Cuts at:   2404    2782    2404 
   Size:        378    4717
 
FspI TGC'GCA

Cuts at:   3601    3601 
   Size:       5095
 
HaeII r_GCGC'y

Cuts at:   1678    2148    4242    1678 
   Size:        470    2094    2531

  Fragments arranged by size: 

                2531    2094     470
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3736    3768    3810    3842    1373 
   Size:         32     135      32    2164      32      42      32    2626

  Fragments arranged by size: 

                2626    2164     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2439    2685     669 
   Size:        988     281     501     246    3079

  Fragments arranged by size: 

                3079     988     501     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2291     748 
   Size:       1543    3552
 
HpaI GTT'AAC

Cuts at:   2685    2685 
   Size:       5095
 
KpnI G_GTAC'C

Cuts at:   2420    2420 
   Size:       5095
 
MfeI C'AATT_G

Cuts at:   2694    2694 
   Size:       5095
 
MluI A'CGCG_T

Cuts at:   2054    4971    2054 
   Size:       2917    2178
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4209    4393    1305 
   Size:       2904     184    2007

  Fragments arranged by size: 

                2904    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3273

  Fragments arranged by size: 

                3273    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3053    3412    3571     518 
   Size:        648    1887     359     159    2042

  Fragments arranged by size: 

                2042    1887     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2165    2511    3188    4336    4581    1606 
   Size:        559     346     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     559     346     245
 
NaeI GCC'GGC

Cuts at:   2143    2143 
   Size:       5095
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4543
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5095
 
NgoMIV G'CCGG_C

Cuts at:   2141    2141 
   Size:       5095
 
NheI G'CTAG_C

Cuts at:   1052    2200    2260    1052 
   Size:       1148      60    3887

  Fragments arranged by size: 

                3887    1148      60
 
NotI GC'GGCC_GC

Cuts at:   2542    2542 
   Size:       5095
 
NruI TCG'CGA

Cuts at:   2220    2220 
   Size:       5095
 
NspI r_CATG'y

Cuts at:   1132    1229    2453    3998    1132 
   Size:         97    1224    1545    2229

  Fragments arranged by size: 

                2229    1545    1224      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5095
 
PciI A'CATG_T

Cuts at:   3994    3994 
   Size:       5095
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5095
 
PmeI GTTT'AAAC

Cuts at:   2399    2399 
   Size:       5095
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3164    3196    4677    4709    3164 
   Size:         32    1481      32    3550

  Fragments arranged by size: 

                3550    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5095
 
PsiI TTA'TAA

Cuts at:   2665    2665 
   Size:       5095
 
PstI C_TGCA'G

Cuts at:    830    2259    2447     830 
   Size:       1429     188    3478

  Fragments arranged by size: 

                3478    1429     188
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5095
 
SalI G'TCGA_C

Cuts at:   1936    2437    1936 
   Size:        501    4594
 
SbfI CC_TGCA'GG

Cuts at:   2447    2447 
   Size:       5095
 
ScaI AGT'ACT

Cuts at:   3343    5055    3343 
   Size:       1712    3383
 
SfcI C'TryA_G

Cuts at:    826    1045    2255    2443    3578    4259    4450     826 
   Size:        219    1210     188    1135     681     191    1471

  Fragments arranged by size: 

                1471    1210    1135     681     219     191     188
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5095
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5095
 
SmaI CCC'GGG

Cuts at:   1872    2422    1872 
   Size:        550    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3199    4100    4362     820 
   Size:        197     926    1256     901     262    1553

  Fragments arranged by size: 

                1553    1256     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2886     493 
   Size:       2393    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5095
 
SphI G_CATG'C

Cuts at:   1132    2453    1132 
   Size:       1321    3774
 
SspI AAT'ATT

Cuts at:      5      52    3019       5 
   Size:         47    2967    2081

  Fragments arranged by size: 

                2967    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5095
 
StyI C'CwwG_G

Cuts at:    513    1065    2385    2534     513 
   Size:        552    1320     149    3074

  Fragments arranged by size: 

                3074    1320     552     149
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3122    3139    3292    3477    3922     325 
   Size:       1255    1542      17     153     185     445    1498

  Fragments arranged by size: 

                1542    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3341    5053      96 
   Size:        274      80      33      51    1443    1364    1712     138

  Fragments arranged by size: 

                1712    1443    1364     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5055    5087    5055 
   Size:         32    5063
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5095
 
XbaI T'CTAG_A

Cuts at:   2431    2431 
   Size:       5095
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5095
 
XmaI C'CCGG_G

Cuts at:   1870    2420    1870 
   Size:        550    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3224    3224 
   Size:       5095
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2903     276 
   Size:         53      83     186     936    1369    2468

  Fragments arranged by size: 

                2468    1369     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI     SalI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NarI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI
    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SapI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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