(Circular) (Six-base) MAPSORT of: pFN21AE1617.seq Check: 4579 from: 1 to: 5086 pFN21AE1617 5086 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2896 278 Size: 53 83 186 936 1360 2468 Fragments arranged by size: 2468 1360 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2429 1937 Size: 492 4594 Acc65I G'GTAC_C Cuts at: 2407 2407 Size: 5086 AclI AA'CG_TT Cuts at: 1563 3213 3586 1563 Size: 1650 373 3063 Fragments arranged by size: 3063 1650 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3149 4533 4801 1632 Size: 1517 1384 268 1917 Fragments arranged by size: 1917 1517 1384 268 AfeI AGC'GCT Cuts at: 2267 2267 Size: 5086 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4889 AflIII A'CryG_T Cuts at: 1203 3985 4962 1203 Size: 2782 977 1327 Fragments arranged by size: 2782 1327 977 AgeI A'CCGG_T Cuts at: 3917 3917 Size: 5086 AhdI GACnn_n'nnGTC Cuts at: 3815 3815 Size: 5086 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5086 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5086 AlwNI CAG_nnn'CTG Cuts at: 2829 4401 2829 Size: 1572 3514 ApaLI G'TGCA_C Cuts at: 3143 4299 3143 Size: 1156 3930 ApoI r'AATT_y Cuts at: 1493 2197 2395 2621 2773 4882 1493 Size: 704 198 226 152 2109 1697 Fragments arranged by size: 2109 1697 704 226 198 152 AseI AT'TA_AT Cuts at: 160 1032 3640 160 Size: 872 2608 1606 Fragments arranged by size: 2608 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5086 AvaI C'yCGr_G Cuts at: 1870 1943 2411 1870 Size: 73 468 4545 Fragments arranged by size: 4545 468 73 BamHI G'GATC_C Cuts at: 2416 2783 2416 Size: 367 4719 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2351 2407 3862 618 Size: 325 217 627 564 56 1455 1842 Fragments arranged by size: 1842 1455 627 564 325 217 56 BanII G_rGCy'C Cuts at: 1391 2405 1391 Size: 1014 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3277 3311 1903 Size: 34 50 34 1256 34 3678 Fragments arranged by size: 3678 1256 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2978 4188 2978 Size: 1210 3876 BclI T'GATC_A Cuts at: 1548 1548 Size: 5086 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3697 136 Size: 107 122 71 631 605 2025 1525 Fragments arranged by size: 2025 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5081 5081 Size: 5086 BlpI GC'TnA_GC Cuts at: 2503 2503 Size: 5086 Bme1580I G_kGCm'C Cuts at: 1790 2354 3147 4303 1790 Size: 564 793 1156 2573 Fragments arranged by size: 2573 1156 793 564 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5086 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2817 3775 442 Size: 416 53 83 399 24 1400 958 1753 Fragments arranged by size: 1753 1400 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5086 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3746 1239 Size: 2507 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3211 4076 4374 3211 Size: 865 298 3923 Fragments arranged by size: 3923 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3749 882 Size: 617 2250 2219 Fragments arranged by size: 2250 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2877 493 Size: 711 1673 2702 Fragments arranged by size: 2702 1673 711 BsaBI GATnn'nnATC Cuts at: 5080 5080 Size: 5086 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2893 3275 3910 275 Size: 53 83 186 936 1360 382 635 1451 Fragments arranged by size: 1451 1360 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2158 3519 3917 4191 4338 4749 2158 Size: 1361 398 274 147 411 2495 Fragments arranged by size: 2495 1361 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2363 2393 1178 Size: 30 165 30 960 30 3871 Fragments arranged by size: 3871 960 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5086 BseYI C'CCAG_C Cuts at: 4289 4785 4289 Size: 496 4590 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4513 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2536 3297 4325 1540 Size: 275 186 535 761 1028 2301 Fragments arranged by size: 2301 1028 761 535 275 186 BsiHKAI G_wGCw'C Cuts at: 3147 3232 4303 3147 Size: 85 1071 3930 Fragments arranged by size: 3930 1071 85 BsmI GAATG_Cn' Cuts at: 2597 2690 2597 Size: 93 4993 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2354 2405 3147 3232 4303 1391 Size: 399 564 51 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 564 399 85 51 BspEI T'CCGG_A Cuts at: 2158 4749 2158 Size: 2591 2495 BspHI T'CATG_A Cuts at: 2975 4705 2975 Size: 1730 3356 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2140 2441 844 Size: 1296 301 3489 Fragments arranged by size: 3489 1296 301 BsrBI CCG'CTC Cuts at: 1394 1823 2973 1394 Size: 429 1150 3507 Fragments arranged by size: 3507 1150 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3581 3755 65 Size: 1183 2333 174 1396 Fragments arranged by size: 2333 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3730 3917 1700 Size: 110 1920 187 2869 Fragments arranged by size: 2869 1920 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5086 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5086 BssSI C'ACGA_G Cuts at: 3146 4158 3146 Size: 1012 4074 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5086 BstBI TT'CG_AA Cuts at: 2177 3890 2177 Size: 1713 3373 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5086 BstYI r'GATC_y Cuts at: 1972 2416 2783 3168 3185 4637 4723 4735 5081 Size: 444 367 385 17 1452 86 12 346 Cuts at: 5081 1972 Size: 1977 Fragments arranged by size: 1977 1452 444 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4534 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4347 Fragments arranged by size: 4347 683 56 BtsI GCAGTG_nn' Cuts at: 2307 2602 3396 3416 2307 Size: 295 794 20 3977 Fragments arranged by size: 3977 794 295 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5086 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5051 DraI TTT'AAA Cuts at: 2390 2390 Size: 5086 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4093 809 Size: 507 2777 1802 Fragments arranged by size: 2777 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2533 3422 5051 Size: 54 1425 50 275 18 703 889 1629 Cuts at: 5051 8 Size: 43 Fragments arranged by size: 1629 1425 889 703 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2533 1537 Size: 275 721 4090 Fragments arranged by size: 4090 721 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2179 3016 1353 Size: 826 837 3423 Fragments arranged by size: 3423 837 826 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3673 4047 4193 387 Size: 1064 138 2084 374 146 1280 Fragments arranged by size: 2084 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3149 3746 4533 4801 1239 Size: 393 1517 597 787 268 1524 Fragments arranged by size: 1524 1517 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2103 2103 Size: 5086 EcoRI G'AATT_C Cuts at: 2197 2395 2773 2197 Size: 198 378 4510 Fragments arranged by size: 4510 378 198 EcoRV GAT'ATC Cuts at: 2215 2215 Size: 5086 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2065 2065 Size: 5086 FspI TGC'GCA Cuts at: 3592 3592 Size: 5086 HaeII r_GCGC'y Cuts at: 1678 2269 4233 1678 Size: 591 1964 2531 Fragments arranged by size: 2531 1964 591 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 3727 3759 3801 Size: 32 135 32 431 32 1692 32 42 Cuts at: 3801 3833 1373 Size: 32 2626 Fragments arranged by size: 2626 1692 431 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2430 2676 669 Size: 988 281 492 246 3079 Fragments arranged by size: 3079 988 492 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5086 HpaI GTT'AAC Cuts at: 2676 2676 Size: 5086 KpnI G_GTAC'C Cuts at: 2411 2411 Size: 5086 MfeI C'AATT_G Cuts at: 2685 2685 Size: 5086 MluI A'CGCG_T Cuts at: 4962 4962 Size: 5086 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4200 4384 1305 Size: 2895 184 2007 Fragments arranged by size: 2895 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3264 Fragments arranged by size: 3264 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3044 3403 3562 518 Size: 648 1878 359 159 2042 Fragments arranged by size: 2042 1878 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2502 3179 4327 4572 1606 Size: 896 677 1148 245 2120 Fragments arranged by size: 2120 1148 896 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4534 NdeI CA'TA_TG Cuts at: 387 387 Size: 5086 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5086 NotI GC'GGCC_GC Cuts at: 2533 2533 Size: 5086 NspI r_CATG'y Cuts at: 1132 1229 2339 2444 3989 1132 Size: 97 1110 105 1545 2229 Fragments arranged by size: 2229 1545 1110 105 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5086 PciI A'CATG_T Cuts at: 3985 3985 Size: 5086 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5086 PmeI GTTT'AAAC Cuts at: 2390 2390 Size: 5086 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3155 3187 4668 4700 3155 Size: 32 1481 32 3541 Fragments arranged by size: 3541 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2103 2103 Size: 5086 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5086 PsiI TTA'TAA Cuts at: 2656 2656 Size: 5086 PstI C_TGCA'G Cuts at: 830 2438 830 Size: 1608 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5086 SalI G'TCGA_C Cuts at: 1936 2428 1936 Size: 492 4594 SbfI CC_TGCA'GG Cuts at: 2438 2438 Size: 5086 ScaI AGT'ACT Cuts at: 3334 5046 3334 Size: 1712 3374 SfcI C'TryA_G Cuts at: 826 1045 2257 2434 3569 4250 4441 826 Size: 219 1212 177 1135 681 191 1471 Fragments arranged by size: 1471 1212 1135 681 219 191 177 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5086 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5086 SmaI CCC'GGG Cuts at: 1872 2413 1872 Size: 541 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3190 4091 4353 820 Size: 197 926 1247 901 262 1553 Fragments arranged by size: 1553 1247 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2877 493 Size: 2384 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5086 SphI G_CATG'C Cuts at: 1132 2444 1132 Size: 1312 3774 SspI AAT'ATT Cuts at: 5 52 3010 5 Size: 47 2958 2081 Fragments arranged by size: 2958 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5086 StyI C'CwwG_G Cuts at: 513 1065 2355 2382 2525 513 Size: 552 1290 27 143 3074 Fragments arranged by size: 3074 1290 552 143 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3113 3130 3283 3468 3913 325 Size: 1255 1533 17 153 185 445 1498 Fragments arranged by size: 1533 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2370 3332 5044 Size: 274 80 33 51 1443 393 962 1712 Cuts at: 5044 96 Size: 138 Fragments arranged by size: 1712 1443 962 393 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5046 5078 5046 Size: 32 5054 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5086 XbaI T'CTAG_A Cuts at: 2422 2422 Size: 5086 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2162 2162 Size: 5086 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5086 XmaI C'CCGG_G Cuts at: 1870 2411 1870 Size: 541 4545 XmnI GAAnn'nnTTC Cuts at: 2201 3215 2201 Size: 1014 4072 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2894 276 Size: 53 83 186 936 1360 2468 Fragments arranged by size: 2468 1360 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI