Restriction map for
pFN21AE2049
(Linear) (Six-base) MAPSORT of: KIEE2049.seq Check: 9252 from: 1 to: 2013
KIEE2049.orf
With 182 enzymes: SgfI *
June 3, 2018 14:24 ..
AatII G_ACGT'C
Cuts at: 0 602 2013
Size: 602 1411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 2013
Size: 436 1577
AflIII A'CryG_T
Cuts at: 0 11 2013
Size: 11 2002
AhdI GACnn_n'nnGTC
Cuts at: 0 1240 2013
Size: 1240 773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 316 2013
Size: 316 1697
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1437 1469 2013
Size: 1437 32 544
Fragments arranged by size:
1437 544 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1516 2013
Size: 1516 497
ApaI G_GGCC'C
Cuts at: 0 152 2013
Size: 152 1861
ApaLI G'TGCA_C
Cuts at: 0 1345 2013
Size: 1345 668
ApoI r'AATT_y
Cuts at: 0 850 999 1281 2013
Size: 850 149 282 732
Fragments arranged by size:
850 732 282 149
AvaI C'yCGr_G
Cuts at: 0 583 2013
Size: 583 1430
BamHI G'GATC_C
Cuts at: 0 1437 2013
Size: 1437 576
BanI G'GyrC_C
Cuts at: 0 541 562 579 2013
Size: 541 21 17 1434
Fragments arranged by size:
1434 541 21 17
BanII G_rGCy'C
Cuts at: 0 152 1115 1364 2013
Size: 152 963 249 649
Fragments arranged by size:
963 649 249 152
BbeI G_GCGC'C
Cuts at: 0 583 2013
Size: 583 1430
BbvCI CC'TCA_GC
Cuts at: 0 526 2013
Size: 526 1487
BclI T'GATC_A
Cuts at: 0 125 2013
Size: 125 1888
BglI GCCn_nnn'nGGC
Cuts at: 0 1359 2013
Size: 1359 654
Bme1580I G_kGCm'C
Cuts at: 0 152 1349 1806 2013
Size: 152 1197 457 207
Fragments arranged by size:
1197 457 207 152
BmgBI CAC'GTC
Cuts at: 0 900 2013
Size: 900 1113
BmrI ACTGGGnnnn_n'
Cuts at: 0 1504 1822 2013
Size: 1504 318 191
Fragments arranged by size:
1504 318 191
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 1108 2013
Size: 73 1035 905
Fragments arranged by size:
1035 905 73
Bpu10I CC'TnA_GC
Cuts at: 0 526 2013
Size: 526 1487
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1361 2013
Size: 1361 652
BsaI GGTCTCn'nnnn_
Cuts at: 0 1248 1513 2013
Size: 1248 265 500
Fragments arranged by size:
1248 500 265
BsaAI yAC'GTr
Cuts at: 0 306 2013
Size: 306 1707
BsaHI Gr'CG_yC
Cuts at: 0 239 580 599 2013
Size: 239 341 19 1414
Fragments arranged by size:
1414 341 239 19
BsaWI w'CCGG_w
Cuts at: 0 813 2013
Size: 813 1200
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1057 1087 1919 1949 2013
Size: 1057 30 832 30 64
Fragments arranged by size:
1057 832 64 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 1078 1237 2013
Size: 199 879 159 776
Fragments arranged by size:
879 776 199 159
BseYI C'CCAG_C
Cuts at: 0 800 1114 2013
Size: 800 314 899
Fragments arranged by size:
899 800 314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 1012 1343 2013
Size: 80 932 331 670
Fragments arranged by size:
932 670 331 80
BsiEI CG_ry'CG
Cuts at: 0 826 2013
Size: 826 1187
BsiHKAI G_wGCw'C
Cuts at: 0 167 518 590 1094 1349 2013
Size: 167 351 72 504 255 664
Fragments arranged by size:
664 504 351 255 167 72
BsmI GAATG_Cn'
Cuts at: 0 956 1613 2013
Size: 956 657 400
Fragments arranged by size:
956 657 400
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1227 2013
Size: 1227 786
Bsp1286I G_dGCh'C
Cuts at: 0 152 167 518 590 1094 1115 1349 1364
Size: 152 15 351 72 504 21 234 15
Cuts at: 1364 1806 2013
Size: 442 207
Fragments arranged by size:
504 442 351 234 207 152 72 21
15 15
BspEI T'CCGG_A
Cuts at: 0 813 2013
Size: 813 1200
BspHI T'CATG_A
Cuts at: 0 122 128 788 2013
Size: 122 6 660 1225
Fragments arranged by size:
1225 660 122 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1441 2013
Size: 1441 572
BsrFI r'CCGG_y
Cuts at: 0 992 2013
Size: 992 1021
BsrGI T'GTAC_A
Cuts at: 0 140 632 2013
Size: 140 492 1381
Fragments arranged by size:
1381 492 140
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1045 1159 2013
Size: 1045 114 854
Fragments arranged by size:
1045 854 114
BstYI r'GATC_y
Cuts at: 0 1437 2013
Size: 1437 576
Bsu36I CC'TnA_GG
Cuts at: 0 1356 2013
Size: 1356 657
BtgI C'CryG_G
Cuts at: 0 1931 2013
Size: 1931 82
BtsI GCAGTG_nn'
Cuts at: 0 1024 1307 1349 2013
Size: 1024 283 42 664
Fragments arranged by size:
1024 664 283 42
DraI TTT'AAA
Cuts at: 0 911 2013
Size: 911 1102
EaeI y'GGCC_r
Cuts at: 0 823 2013
Size: 823 1190
EagI C'GGCC_G
Cuts at: 0 823 2013
Size: 823 1190
EarI CTCTTCn'nnn_
Cuts at: 0 506 887 898 940 965 1097 1691 2013
Size: 506 381 11 42 25 132 594 322
Fragments arranged by size:
594 506 381 322 132 42 25 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 436 1108 2013
Size: 73 363 672 905
Fragments arranged by size:
905 672 363 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 181 2013
Size: 181 1832
EcoO109I rG'GnC_Cy
Cuts at: 0 148 174 411 1054 1453 2013
Size: 148 26 237 643 399 560
Fragments arranged by size:
643 560 399 237 148 26
HaeII r_GCGC'y
Cuts at: 0 583 1957 2013
Size: 583 1374 56
Fragments arranged by size:
1374 583 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 312 344 764 796 1226 1258
Size: 110 32 170 32 420 32 430 32
Cuts at: 1258 1512 1544 2013
Size: 254 32 469
Fragments arranged by size:
469 430 420 254 170 110 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 603 2013
Size: 603 1410
KasI G'GCGC_C
Cuts at: 0 579 2013
Size: 579 1434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 2013
Size: 605 1408
MslI CAynn'nnrTG
Cuts at: 0 31 127 593 1157 1337 1853 2013
Size: 31 96 466 564 180 516 160
Fragments arranged by size:
564 516 466 180 160 96 31
MspA1I CmG'CkG
Cuts at: 0 74 1705 2013
Size: 74 1631 308
Fragments arranged by size:
1631 308 74
NarI GG'CG_CC
Cuts at: 0 580 2013
Size: 580 1433
NdeI CA'TA_TG
Cuts at: 0 681 1487 2013
Size: 681 806 526
Fragments arranged by size:
806 681 526
NspI r_CATG'y
Cuts at: 0 15 2013
Size: 15 1998
PasI CC'CwG_GG
Cuts at: 0 114 1038 2013
Size: 114 924 975
Fragments arranged by size:
975 924 114
PciI A'CATG_T
Cuts at: 0 11 2013
Size: 11 2002
PflMI CCAn_nnn'nTGG
Cuts at: 0 1463 2013
Size: 1463 550
PfoI T'CCnGG_A
Cuts at: 0 50 1440 1969 2013
Size: 50 1390 529 44
Fragments arranged by size:
1390 529 50 44
PmlI CAC'GTG
Cuts at: 0 306 2013
Size: 306 1707
PpuMI rG'GwC_Cy
Cuts at: 0 411 1054 1453 2013
Size: 411 643 399 560
Fragments arranged by size:
643 560 411 399
PspOMI G'GGCC_C
Cuts at: 0 148 2013
Size: 148 1865
PstI C_TGCA'G
Cuts at: 0 1124 2013
Size: 1124 889
PvuII CAG'CTG
Cuts at: 0 1705 2013
Size: 1705 308
SapI GCTCTTCn'nnn_
Cuts at: 0 506 1691 2013
Size: 506 1185 322
Fragments arranged by size:
1185 506 322
ScaI AGT'ACT
Cuts at: 0 262 327 650 763 1382 1608 2013
Size: 262 65 323 113 619 226 405
Fragments arranged by size:
619 405 323 262 226 113 65
SfcI C'TryA_G
Cuts at: 0 644 1120 2013
Size: 644 476 893
Fragments arranged by size:
893 644 476
SfoI GGC'GCC
Cuts at: 0 581 2013
Size: 581 1432
SmlI C'TyrA_G
Cuts at: 0 1376 2013
Size: 1376 637
SpeI A'CTAG_T
Cuts at: 0 1738 2013
Size: 1738 275
StuI AGG'CCT
Cuts at: 0 858 2013
Size: 858 1155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1109 1557 1893 2013
Size: 1109 448 336 120
Fragments arranged by size:
1109 448 336 120
TatI w'GTAC_w
Cuts at: 0 140 260 278 325 632 648 761 1380
Size: 140 120 18 47 307 16 113 619
Cuts at: 1380 1606 2013
Size: 226 407
Fragments arranged by size:
619 407 307 226 140 120 113 47
18 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 383 415 1785 1817 1919 1951 1983 2013
Size: 383 32 1370 32 102 32 32 30
Fragments arranged by size:
1370 383 102 32 32 32 32 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 48 1203 2013
Size: 48 1155 810
Fragments arranged by size:
1155 810 48
ZraI GAC'GTC
Cuts at: 0 600 2013
Size: 600 1413
Enzymes that do cut:
AatII AcuI AflIII AhdI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbvCI
BclI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrFI BsrGI BstXI BstYI Bsu36I BtgI BtsI DraI
EaeI EagI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII KasI MmeI MslI MspA1I NarI NdeI NspI
PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI
PvuII SapI ScaI SfcI SfoI SmlI SpeI StuI
TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AleI
AscI AseI AsiSI AvrII BaeI BbsI BcgI BciVI
BfrBI BglII BlpI BmtI BplI BsaBI BsiWI BsrBI
BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraIII DrdI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MscI NaeI NcoI NgoMIV NheI NotI NruI
NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI SexAI
SfiI SgfI SgrAI SmaI SnaBI SphI SrfI SspI
StyI SwaI Tth111I XbaI XhoI XmaI XmnI