################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflII AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI BstAPI BstBI BstYI Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MaeI MaeII MflI MluI MroI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 541 GTATAC GT^[AC][GT]AC AccII 756 CGCG CG^CG AgeI 344 ACCGGT A^CCGGT AspHI 685 GAGCAC G[AT]GC[AT]^C AvaI 626 CCCGGG C^[CT]CG[GA]G BalI 920 TGGCCA TGG^CCA BbsI 265 GTCTTC GAAGAC(2/6) BcgI 104 GCAATGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 685 GAGCAC G[AGT]GC[ACT]^C BpmI 1024 CTCCAG CTGGAG(16/14) BpuAI 265 GTCTTC GAAGAC(2/6) BsaBI 54 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 487 GAGACC GGTCTC(1/5) BsaWI 344 ACCGGT [AT]^CCGG[AT] BseRI 196 CTCCTC GAGGAG(10/8) BsgI 303 CTGCAC GTGCAG(16/14) BsiEI 342 CGACCG CG[GA][CT]^CG BsiHKAI 685 GAGCAC G[AT]GC[AT]^C BsmFI 1039 GGGAC GGGAC(10/14) BsmI 1096 GAATGC GAATGC(1/-1) BsoBI 626 CCCGGG C^[CT]CG[GA]G Bsp1286I 685 GAGCAC G[AGT]GC[ACT]^C BspHI 329 TCATGA T^CATGA BspMI 191 ACCTGC ACCTGC(4/8) BsrDI 104 GCAATG GCAATG(2/0) Bst1107I 541 GTATAC GTA^TAC BstUI 756 CGCG CG^CG BstZ17I 541 GTATAC GTA^TAC FauI 579 CCCGC CCCGC(4/6) HinfI 37 GACTC G^A[ACGT]TC MamI 54 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 159 CAATTG C^AATTG MluNI 920 TGGCCA TGG^CCA MscI 920 TGGCCA TGG^CCA MslI 60 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 159 CAATTG C^AATTG MvnI 756 CGCG CG^CG NaeI 140 GCCGGC GCC^GGC NcoI 1078 CCATGG C^CATGG NgoMIV 140 GCCGGC G^CCGGC PinAI 344 ACCGGT A^CCGGT PleI 37 GACTC GAGTC(4/5) PpuMI 976 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1041 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 912 CTGCAG CTGCA^G RcaI 329 TCATGA T^CATGA SapI 903 GCTCTTC GCTCTTC(1/4) SfcI 912 CTGCAG C^T[GA][CT]AG SmaI 626 CCCGGG CCC^GGG SphI 46 GCATGC GCATG^C TaqI 341 TCGA T^CGA TthHB8I 341 TCGA T^CGA XmaCI 626 CCCGGG C^CCGGG XmaI 626 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 374 GGTACC G^GTACC 893 GGTACC AlwI 630 GGATC GGATC(4/5) 644 GGATC Asp718I 374 GGTACC G^GTACC 893 GGTACC BclI 332 TGATCA T^GATCA 587 TGATCA BsmAI 487 GAGAC GTCTC(1/5) 878 GAGAC BsrFI 140 GCCGGC [GA]^CCGG[CT] 344 ACCGGT BsrI 705 ACTGG ACTGG(1/-1) 394 CCAGT BstEII 387 GGTCACC G^GT[ACGT]ACC 564 GGTCACC BstPI 387 GGTCACC G^GT[ACGT]ACC 564 GGTCACC CfoI 353 GCGC GCG^C 868 GCGC Cfr10I 140 GCCGGC [GA]^CCGG[CT] 344 ACCGGT DraII 721 GGGGCCT [GA]G^G[ACGT]CC[CT] 976 AGGACCC EcoO109I 721 GGGGCCT [GA]G^G[ACGT]CC[CT] 976 AGGACCC EcoO65I 387 GGTCACC G^GT[ACGT]ACC 564 GGTCACC EcoT14I 176 CCTTGG C^C[AT][AT]GG 1078 CCATGG FbaI 332 TGATCA T^GATCA 587 TGATCA HhaI 353 GCGC GCG^C 868 GCGC HinP1I 353 GCGC G^CGC 868 GCGC KpnI 374 GGTACC GGTAC^C 893 GGTACC MseI 320 TTAA T^TAA 421 TTAA MspA1I 235 CAGCTG C[AC]G^C[GT]G 967 CAGCTG NspI 46 GCATGC [GA]CATG^[CT] 1177 ACATGC PvuII 235 CAGCTG CAG^CTG 967 CAGCTG StyI 176 CCTTGG C^C[AT][AT]GG 1078 CCATGG Tru9I 320 TTAA T^TAA 421 TTAA XcmI 695 CCACAGTGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1026 CCAGGTGTGGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AfaI AluI AvaII BanI BbvI BcnI BsaJI BsiYI BslI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI