(Circular) (Six-base) MAPSORT of: pFN21AB7036.seq Check: 9932 from: 1 to: 5896 pFN21AB7036 5896 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3706 278 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2547 3239 1937 Size: 610 692 4594 Fragments arranged by size: 4594 692 610 Acc65I G'GTAC_C Cuts at: 2379 2898 3217 2379 Size: 519 319 5058 Fragments arranged by size: 5058 519 319 AclI AA'CG_TT Cuts at: 1563 4023 4396 1563 Size: 2460 373 3063 Fragments arranged by size: 3063 2460 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3044 3959 5343 5611 1632 Size: 1412 915 1384 268 1917 Fragments arranged by size: 1917 1412 1384 915 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5699 AflIII A'CryG_T Cuts at: 1203 4795 5772 1203 Size: 3592 977 1327 Fragments arranged by size: 3592 1327 977 AgeI A'CCGG_T Cuts at: 2349 4727 2349 Size: 2378 3518 AhdI GACnn_n'nnGTC Cuts at: 4625 4625 Size: 5896 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5896 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5896 AlwNI CAG_nnn'CTG Cuts at: 3639 5211 3639 Size: 1572 4324 ApaLI G'TGCA_C Cuts at: 3953 5109 3953 Size: 1156 4740 ApoI r'AATT_y Cuts at: 1493 3205 3431 3583 5692 1493 Size: 1712 226 152 2109 1697 Fragments arranged by size: 2109 1712 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4450 160 Size: 872 3418 1606 Fragments arranged by size: 3418 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 AvaI C'yCGr_G Cuts at: 1870 1943 2631 3221 1870 Size: 73 688 590 4545 Fragments arranged by size: 4545 688 590 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2363 2396 2363 Size: 33 5863 BamHI G'GATC_C Cuts at: 3226 3593 3226 Size: 367 5529 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2379 2898 3217 4672 Size: 325 217 627 221 371 519 319 1455 Cuts at: 4672 618 Size: 1842 Fragments arranged by size: 1842 1455 627 519 371 325 319 221 217 BanII G_rGCy'C Cuts at: 1391 3215 1391 Size: 1824 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2263 928 Size: 971 364 4561 Fragments arranged by size: 4561 971 364 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2098 2132 4087 4121 1903 Size: 34 161 34 1955 34 3678 Fragments arranged by size: 3678 1955 161 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3788 4998 3788 Size: 1210 4686 BclI T'GATC_A Cuts at: 1548 2337 2592 1548 Size: 789 255 4852 Fragments arranged by size: 4852 789 255 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4507 136 Size: 107 122 71 631 605 2835 1525 Fragments arranged by size: 2835 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5891 5891 Size: 5896 BlpI GC'TnA_GC Cuts at: 3313 3313 Size: 5896 Bme1580I G_kGCm'C Cuts at: 1790 3957 5113 1790 Size: 2167 1156 2573 Fragments arranged by size: 2573 2167 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5896 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2393 3627 4585 Size: 416 53 83 399 24 976 1234 958 Cuts at: 4585 442 Size: 1753 Fragments arranged by size: 1753 1234 976 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5896 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3014 4556 1239 Size: 1775 1542 2579 Fragments arranged by size: 2579 1775 1542 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4021 4886 5184 4021 Size: 865 298 4733 Fragments arranged by size: 4733 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2486 4559 882 Size: 617 987 2073 2219 Fragments arranged by size: 2219 2073 987 617 BsaAI yAC'GTr Cuts at: 493 1204 3687 493 Size: 711 2483 2702 Fragments arranged by size: 2702 2483 711 BsaBI GATnn'nnATC Cuts at: 2063 5890 2063 Size: 3827 2069 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3703 4085 4720 275 Size: 53 83 186 936 2170 382 635 1451 Fragments arranged by size: 2170 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2349 4329 4727 5001 5148 5559 2349 Size: 1980 398 274 147 411 2686 Fragments arranged by size: 2686 1980 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2648 2678 2921 2951 1178 Size: 30 165 30 1245 30 243 30 4123 Fragments arranged by size: 4123 1245 243 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 1186 Size: 1006 4890 BseYI C'CCAG_C Cuts at: 2830 5099 5595 2830 Size: 2269 496 3131 Fragments arranged by size: 3131 2269 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2293 1163 Size: 573 557 4766 Fragments arranged by size: 4766 573 557 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2350 3346 4107 5135 1540 Size: 275 186 349 996 761 1028 2301 Fragments arranged by size: 2301 1028 996 761 349 275 186 BsiHKAI G_wGCw'C Cuts at: 2694 3957 4042 5113 2694 Size: 1263 85 1071 3477 Fragments arranged by size: 3477 1263 1071 85 BsmI GAATG_Cn' Cuts at: 3107 3407 3500 3107 Size: 300 93 5503 Fragments arranged by size: 5503 300 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2694 3215 3957 4042 5113 1391 Size: 399 904 521 742 85 1071 2174 Fragments arranged by size: 2174 1071 904 742 521 399 85 BspEI T'CCGG_A Cuts at: 5559 5559 Size: 5896 BspHI T'CATG_A Cuts at: 2334 3785 5515 2334 Size: 1451 1730 2715 Fragments arranged by size: 2715 1730 1451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2205 3251 844 Size: 1361 1046 3489 Fragments arranged by size: 3489 1361 1046 BsrBI CCG'CTC Cuts at: 1394 1823 3783 1394 Size: 429 1960 3507 Fragments arranged by size: 3507 1960 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2116 4391 4565 65 Size: 1183 868 2275 174 1396 Fragments arranged by size: 2275 1396 1183 868 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2145 2349 4540 4727 1700 Size: 110 335 204 2191 187 2869 Fragments arranged by size: 2869 2191 335 204 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5896 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5896 BssSI C'ACGA_G Cuts at: 3956 4968 3956 Size: 1012 4884 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5896 BstBI TT'CG_AA Cuts at: 4700 4700 Size: 5896 BstEII G'GTnAC_C Cuts at: 1184 2392 2569 1184 Size: 1208 177 4511 Fragments arranged by size: 4511 1208 177 BstXI CCAn_nnnn'nTGG Cuts at: 2071 2419 3105 2071 Size: 348 686 4862 Fragments arranged by size: 4862 686 348 BstYI r'GATC_y Cuts at: 1972 3226 3593 3978 3995 5447 5533 5545 5891 Size: 1254 367 385 17 1452 86 12 346 Cuts at: 5891 1972 Size: 1977 Fragments arranged by size: 1977 1452 1254 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2548 2548 Size: 5896 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 2130 2773 3083 513 Size: 552 939 66 60 643 310 3326 Fragments arranged by size: 3326 939 643 552 310 66 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5157 Fragments arranged by size: 5157 683 56 BtsI GCAGTG_nn' Cuts at: 2372 3412 4206 4226 2372 Size: 1040 794 20 4042 Fragments arranged by size: 4042 1040 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5896 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5861 DraI TTT'AAA Cuts at: 3200 3200 Size: 5896 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4903 809 Size: 507 3587 1802 Fragments arranged by size: 3587 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2712 2925 Size: 54 1425 50 275 18 297 585 213 Cuts at: 2925 3343 4232 5861 8 Size: 418 889 1629 43 Fragments arranged by size: 1629 1425 889 585 418 297 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3343 1537 Size: 275 1531 4090 Fragments arranged by size: 4090 1531 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2032 2336 2915 3006 3826 1353 Size: 679 304 579 91 820 3423 Fragments arranged by size: 3423 820 679 579 304 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4483 4857 5003 387 Size: 1064 138 2894 374 146 1280 Fragments arranged by size: 2894 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3014 3044 3959 4556 5343 5611 1239 Size: 393 1382 30 915 597 787 268 1524 Fragments arranged by size: 1524 1382 915 787 597 393 268 30 EcoO109I rG'GnC_Cy Cuts at: 2727 2982 2727 Size: 255 5641 EcoRI G'AATT_C Cuts at: 3205 3583 3205 Size: 378 5518 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2275 2275 Size: 5896 FspI TGC'GCA Cuts at: 4402 4402 Size: 5896 HaeII r_GCGC'y Cuts at: 1678 5043 1678 Size: 3365 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2638 2670 4537 4569 4611 Size: 32 135 32 1066 32 1867 32 42 Cuts at: 4611 4643 1373 Size: 32 2626 Fragments arranged by size: 2626 1867 1066 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3240 3486 669 Size: 988 281 1302 246 3079 Fragments arranged by size: 3079 1302 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5896 HpaI GTT'AAC Cuts at: 3486 3486 Size: 5896 KpnI G_GTAC'C Cuts at: 2383 2902 3221 2383 Size: 519 319 5058 Fragments arranged by size: 5058 519 319 MfeI C'AATT_G Cuts at: 2164 3495 2164 Size: 1331 4565 MluI A'CGCG_T Cuts at: 5772 5772 Size: 5896 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2985 5010 5194 1305 Size: 1680 2025 184 2007 Fragments arranged by size: 2025 2007 1680 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2927 10 Size: 54 1425 343 1095 2979 Fragments arranged by size: 2979 1425 1095 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2069 3854 4213 4372 518 Size: 648 903 1785 359 159 2042 Fragments arranged by size: 2042 1785 903 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2242 2974 3312 3989 5137 5382 1606 Size: 636 732 338 677 1148 245 2120 Fragments arranged by size: 2120 1148 732 677 636 338 245 NaeI GCC'GGC Cuts at: 2147 2147 Size: 5896 NcoI C'CATG_G Cuts at: 513 1065 2004 3083 513 Size: 552 939 1079 3326 Fragments arranged by size: 3326 1079 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5896 NgoMIV G'CCGG_C Cuts at: 2145 2145 Size: 5896 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5896 NotI GC'GGCC_GC Cuts at: 3343 3343 Size: 5896 NspI r_CATG'y Cuts at: 1132 1229 2055 3186 3254 4799 1132 Size: 97 826 1131 68 1545 2229 Fragments arranged by size: 2229 1545 1131 826 97 68 PasI CC'CwG_GG Cuts at: 1348 3064 1348 Size: 1716 4180 PciI A'CATG_T Cuts at: 4795 4795 Size: 5896 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5896 PfoI T'CCnGG_A Cuts at: 2666 2978 2666 Size: 312 5584 PmeI GTTT'AAAC Cuts at: 3200 3200 Size: 5896 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3965 3997 5478 5510 3965 Size: 32 1481 32 4351 Fragments arranged by size: 4351 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2982 2982 Size: 5896 PshAI GACnn'nnGTC Cuts at: 1613 3050 1613 Size: 1437 4459 PsiI TTA'TAA Cuts at: 3466 3466 Size: 5896 PstI C_TGCA'G Cuts at: 830 2921 3248 830 Size: 2091 327 3478 Fragments arranged by size: 3478 2091 327 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5896 PvuII CAG'CTG Cuts at: 2242 2974 2242 Size: 732 5164 SalI G'TCGA_C Cuts at: 1936 3238 1936 Size: 1302 4594 SapI GCTCTTCn'nnn_ Cuts at: 2915 2915 Size: 5896 SbfI CC_TGCA'GG Cuts at: 3248 3248 Size: 5896 ScaI AGT'ACT Cuts at: 4144 5856 4144 Size: 1712 4184 SfcI C'TryA_G Cuts at: 826 1045 2917 3244 4379 5060 5251 826 Size: 219 1872 327 1135 681 191 1471 Fragments arranged by size: 1872 1471 1135 681 327 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5896 SmaI CCC'GGG Cuts at: 1872 2633 3223 1872 Size: 761 590 4545 Fragments arranged by size: 4545 761 590 SmlI C'TyrA_G Cuts at: 820 1017 1943 4000 4901 5163 820 Size: 197 926 2057 901 262 1553 Fragments arranged by size: 2057 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3687 493 Size: 3194 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5896 SphI G_CATG'C Cuts at: 1132 2055 3254 1132 Size: 923 1199 3774 Fragments arranged by size: 3774 1199 923 SspI AAT'ATT Cuts at: 5 52 3820 5 Size: 47 3768 2081 Fragments arranged by size: 3768 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5896 StyI C'CwwG_G Cuts at: 513 1065 2004 2181 3083 3335 513 Size: 552 939 177 902 252 3074 Fragments arranged by size: 3074 939 902 552 252 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 3923 3940 4093 4278 4723 325 Size: 1255 446 1897 17 153 185 445 1498 Fragments arranged by size: 1897 1498 1255 446 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4142 5854 96 Size: 274 80 33 51 1443 2165 1712 138 Fragments arranged by size: 2165 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2648 2680 5856 5888 2648 Size: 32 3176 32 2656 Fragments arranged by size: 3176 2656 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5896 XbaI T'CTAG_A Cuts at: 3232 3232 Size: 5896 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2707 3038 2707 Size: 331 5565 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5896 XmaI C'CCGG_G Cuts at: 1870 2631 3221 1870 Size: 761 590 4545 Fragments arranged by size: 4545 761 590 XmnI GAAnn'nnTTC Cuts at: 4025 4025 Size: 5896 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3704 276 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI