Restriction map for pFN21AB7036
 (Linear) (Six-base) MAPSORT of: KIBB7036.seq  Check: 8601  from: 1  to: 1191



KIBB7036.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:31  ..

AccI GT'mk_AC

Cuts at:      0     542    1191 
   Size:        542     649
 
Acc65I G'GTAC_C

Cuts at:      0     374     893    1191 
   Size:        374     519     298

  Fragments arranged by size: 

                 519     374     298
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1039    1191 
   Size:       1039     152
 
AgeI A'CCGG_T

Cuts at:      0     344    1191 
   Size:        344     847
 
AvaI C'yCGr_G

Cuts at:      0     626    1191 
   Size:        626     565
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     358     391    1191 
   Size:        358      33     800

  Fragments arranged by size: 

                 800     358      33
 
BanI G'GyrC_C

Cuts at:      0       3     374     893    1191 
   Size:          3     371     519     298

  Fragments arranged by size: 

                 519     371     298       3
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     258    1191 
   Size:        258     933
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      93     127    1191 
   Size:         93      34    1064

  Fragments arranged by size: 

                1064      93      34
 
BclI T'GATC_A

Cuts at:      0     332     587    1191 
   Size:        332     255     604

  Fragments arranged by size: 

                 604     332     255
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     388    1191 
   Size:        388     803
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1009    1191 
   Size:       1009     182
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     481    1191 
   Size:        481     710
 
BsaBI GATnn'nnATC

Cuts at:      0      58    1191 
   Size:         58    1133
 
BsaWI w'CCGG_w

Cuts at:      0     344    1191 
   Size:        344     847
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     643     673     916     946    1191 
   Size:        643      30     243      30     245

  Fragments arranged by size: 

                 643     245     243      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     187    1191 
   Size:        187    1004
 
BseYI C'CCAG_C

Cuts at:      0     825    1191 
   Size:        825     366
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     288    1191 
   Size:        288     903
 
BsiEI CG_ry'CG

Cuts at:      0     345    1191 
   Size:        345     846
 
BsiHKAI G_wGCw'C

Cuts at:      0     689    1191 
   Size:        689     502
 
BsmI GAATG_Cn'

Cuts at:      0    1102    1191 
   Size:       1102      89
 
Bsp1286I G_dGCh'C

Cuts at:      0     689    1191 
   Size:        689     502
 
BspHI T'CATG_A

Cuts at:      0     329    1191 
   Size:        329     862
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     200    1191 
   Size:        200     991
 
BsrDI GCAATG_nn'

Cuts at:      0     111    1191 
   Size:        111    1080
 
BsrFI r'CCGG_y

Cuts at:      0     140     344    1191 
   Size:        140     204     847

  Fragments arranged by size: 

                 847     204     140
 
BstEII G'GTnAC_C

Cuts at:      0     387     564    1191 
   Size:        387     177     627

  Fragments arranged by size: 

                 627     387     177
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      66     414    1100    1191 
   Size:         66     348     686      91

  Fragments arranged by size: 

                 686     348      91      66
 
BstZ17I GTA'TAC

Cuts at:      0     543    1191 
   Size:        543     648
 
BtgI C'CryG_G

Cuts at:      0      65     125     768    1078    1191 
   Size:         65      60     643     310     113

  Fragments arranged by size: 

                 643     310     113      65      60
 
BtsI GCAGTG_nn'

Cuts at:      0     367    1191 
   Size:        367     824
 
EaeI y'GGCC_r

Cuts at:      0     122     707     920    1191 
   Size:        122     585     213     271

  Fragments arranged by size: 

                 585     271     213     122
 
EarI CTCTTCn'nnn_

Cuts at:      0      27     331     910    1001    1191 
   Size:         27     304     579      91     190

  Fragments arranged by size: 

                 579     304     190      91      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1009    1039    1191 
   Size:       1009      30     152

  Fragments arranged by size: 

                1009     152      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0     722     977    1191 
   Size:        722     255     214

  Fragments arranged by size: 

                 722     255     214
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     270    1191 
   Size:        270     921
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     633     665    1191 
   Size:        633      32     526

  Fragments arranged by size: 

                 633     526      32
 
KpnI G_GTAC'C

Cuts at:      0     378     897    1191 
   Size:        378     519     294

  Fragments arranged by size: 

                 519     378     294
 
MfeI C'AATT_G

Cuts at:      0     159    1191 
   Size:        159    1032
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     980    1191 
   Size:        980     211
 
MscI TGG'CCA

Cuts at:      0     922    1191 
   Size:        922     269
 
MslI CAynn'nnrTG

Cuts at:      0      64    1191 
   Size:         64    1127
 
MspA1I CmG'CkG

Cuts at:      0     237     969    1191 
   Size:        237     732     222

  Fragments arranged by size: 

                 732     237     222
 
NaeI GCC'GGC

Cuts at:      0     142    1191 
   Size:        142    1049
 
NcoI C'CATG_G

Cuts at:      0    1078    1191 
   Size:       1078     113
 
NgoMIV G'CCGG_C

Cuts at:      0     140    1191 
   Size:        140    1051
 
NspI r_CATG'y

Cuts at:      0      50    1181    1191 
   Size:         50    1131      10

  Fragments arranged by size: 

                1131      50      10
 
PasI CC'CwG_GG

Cuts at:      0    1059    1191 
   Size:       1059     132
 
PfoI T'CCnGG_A

Cuts at:      0     661     973    1191 
   Size:        661     312     218

  Fragments arranged by size: 

                 661     312     218
 
PpuMI rG'GwC_Cy

Cuts at:      0     977    1191 
   Size:        977     214
 
PshAI GACnn'nnGTC

Cuts at:      0    1045    1191 
   Size:       1045     146
 
PstI C_TGCA'G

Cuts at:      0     916    1191 
   Size:        916     275
 
PvuII CAG'CTG

Cuts at:      0     237     969    1191 
   Size:        237     732     222

  Fragments arranged by size: 

                 732     237     222
 
SapI GCTCTTCn'nnn_

Cuts at:      0     910    1191 
   Size:        910     281
 
SfcI C'TryA_G

Cuts at:      0     912    1191 
   Size:        912     279
 
SmaI CCC'GGG

Cuts at:      0     628    1191 
   Size:        628     563
 
SphI G_CATG'C

Cuts at:      0      50    1191 
   Size:         50    1141
 
StyI C'CwwG_G

Cuts at:      0     176    1078    1191 
   Size:        176     902     113

  Fragments arranged by size: 

                 902     176     113
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      21    1191 
   Size:         21    1170
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     643     675    1191 
   Size:        643      32     516

  Fragments arranged by size: 

                 643     516      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     702    1033    1191 
   Size:        702     331     158

  Fragments arranged by size: 

                 702     331     158
 
XmaI C'CCGG_G

Cuts at:      0     626    1191 
   Size:        626     565
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AgeI     AvaI     BaeI     BanI     BbsI
     BcgI     BclI     BmrI     BpmI     BsaI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI    BsrDI    BsrFI   BstEII    BstXI  BstZ17I     BtgI     BtsI
     EaeI     EarI  Eco57MI EcoO109I     FalI    Hin4I     KpnI     MfeI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PasI     PfoI    PpuMI    PshAI     PstI    PvuII     SapI     SfcI
     SmaI     SphI     StyI    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII    BamHI    BanII     BbeI    BbvCI    BciVI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaHI    BsiWI    BsmBI    BspEI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstYI   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     MluI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PmeI     PmlI     PpiI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TatI
  Tth111I     XbaI     XhoI     XmnI     ZraI
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