################################## # NO CUT ENZYMES ( 212 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstNI BstUI BstXI BstYI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoRI EcoRII EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MboII MflI MluI MluNI MroI MscI MseI MspA1I MvaI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AciI 196 CCGC CCGC(-3/-1) AocI 85 CCTAAGG CC^T[ACGT]AGG BanI 69 GGTGCC G^G[CT][GA]CC BbvI 140 GCTGC GCAGC(8/12) BcnI 99 CCGGG CC^[GC]GG BssKI 99 CCGGG ^CC[ACGT]GG BstEII 123 GGTGACC G^GT[ACGT]ACC BstF5I 81 CATCC GGATG(2/0) BstPI 123 GGTGACC G^GT[ACGT]ACC Bsu36I 85 CCTAAGG CC^T[ACGT]AGG DdeI 86 CTAAG C^T[ACGT]AG DraII 90 GGGTCCT [GA]G^G[ACGT]CC[CT] Eco81I 85 CCTAAGG CC^T[ACGT]AGG EcoO109I 90 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoO65I 123 GGTGACC G^GT[ACGT]ACC EcoT14I 73 CCTTGG C^C[AT][AT]GG Fnu4HI 140 GCTGC GC^[ACGT]GC FokI 81 CATCC GGATG(9/13) HapII 99 CCGG C^CGG HpaII 99 CCGG C^CGG HphI 123 GGTGA GGTGA(8/7) ItaI 140 GCTGC GC^[ACGT]GC MaeIII 124 GTGAC ^GT[ACGT]AC MfeI 199 CAATTG C^AATTG MspI 99 CCGG C^CGG MunI 199 CAATTG C^AATTG NciI 99 CCGGG CC^[GC]GG NlaIII 117 CATG CATG^ PpuMI 90 GGGTCCT [GA]G^G[AT]CC[CT] ScrFI 99 CCGGG CC^[ACGT]GG StyI 73 CCTTGG C^C[AT][AT]GG TseI 140 GCTGC G^C[AT]GC Tsp45I 124 GTGAC ^GT[GC]AC TspRI 18 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AvaII 91 GGTCC G^G[AT]CC 193 GGACC BsaJI 73 CCTTGG C^C[ACGT][ACGT]GG 99 CCGGGG BsiYI 94 CCTAACCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 184 CCTCTCTTTGG BslI 94 CCTAACCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 184 CCTCTCTTTGG MwoI 53 GCTTCGTCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 163 GCAATTCTGGC NlaIV 69 GGTGCC GG[ACGT]^[ACGT]CC 90 GGGTCC Tsp509I 165 AATT ^AATT 200 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### Cfr13I HaeIII MnlI MslI Sau96I