################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## Acc65I AccII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaAI BsaBI BsiEI BsiWI BsmBI BsmI BspDI BspHI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI KpnI KspI MamI MfeI MluI MluNI MscI MspA1I MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 1333 GACGTC GACGT^C AccI 1118 GTCTAC GT^[AC][GT]AC AccIII 148 TCCGGA T^CCGGA AfaI 3 GTAC GT^AC Asp700I 510 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 893 CTCGAG C^[CT]CG[GA]G AvrII 846 CCTAGG C^CTAGG BanI 1150 GGCGCC G^G[CT][GA]CC BbeI 1150 GGCGCC GGCGC^C BfaI 847 CTAG C^TAG BlnI 846 CCTAGG C^CTAGG BsaI 37 GAGACC GGTCTC(1/5) BsaWI 148 TCCGGA [AT]^CCGG[AT] BseAI 148 TCCGGA T^CCGGA BseRI 1204 GAGGAG GAGGAG(10/8) BsoBI 893 CTCGAG C^[CT]CG[GA]G Bsp1407I 2 TGTACA T^GTACA BspEI 148 TCCGGA T^CCGGA BsrBI 733 GAGCGG CCGCTC(-3/-3) BsrGI 2 TGTACA T^GTACA BssSI 836 CTCGTG CACGAG(-5/-1) CfoI 1151 GCGC GCG^C DraI 178 TTTAAA TTT^AAA DrdI 253 GACTATAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HaeII 1150 GGCGCC [GA]GCGC^[CT] HhaI 1151 GCGC GCG^C HinP1I 1151 GCGC G^CGC HindIII 555 AAGCTT A^AGCTT HpaI 994 GTTAAC GTT^AAC KasI 1150 GGCGCC G^GCGCC MaeI 847 CTAG C^TAG MroI 148 TCCGGA T^CCGGA NarI 1150 GGCGCC GG^CGCC PaeR7I 893 CTCGAG C^TCGAG PpuMI 183 AGGTCCC [GA]G^G[AT]CC[CT] RsaI 3 GTAC GT^AC SacI 882 GAGCTC GAGCT^C SfoI 1150 GGCGCC GGC^GCC SspBI 2 TGTACA T^GTACA Tsp45I 108 GTCAC ^GT[GC]AC XhoI 893 CTCGAG C^TCGAG XmnI 510 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 513 GAATTT [GA]^AATT[CT] 700 AAATTC AcyI 1150 GGCGCC G[GA]^CG[CT]C 1333 GACGTC AflIII 535 ACATGT A^C[GA][CT]GT 1292 ACATGT ApaI 398 GGGCCC GGGCC^C 1107 GGGCCC ApoI 513 GAATTT [GA]^AATT[CT] 700 AAATTC AspHI 862 GTGCTC G[AT]GC[AT]^C 882 GAGCTC BcnI 446 CCCGG CC^[GC]GG 719 CCCGG BpmI 730 CTGGAG CTGGAG(16/14) 1070 CTGGAG BsaHI 1150 GGCGCC G[GA]^CG[CT]C 1333 GACGTC BsiHKAI 862 GTGCTC G[AT]GC[AT]^C 882 GAGCTC BsmFI 185 GTCCC GGGAC(10/14) 1197 GTCCC BspLU11I 535 ACATGT A^CATGT 1292 ACATGT BsrDI 234 CATTGC GCAATG(2/0) 282 GCAATG BstYI 440 GGATCT [GA]^GATC[CT] 800 GGATCT DraII 183 AGGTCCC [GA]G^G[ACGT]CC[CT] 397 GGGGCCC EcoO109I 183 AGGTCCC [GA]G^G[ACGT]CC[CT] 397 GGGGCCC FauI 296 CCCGC CCCGC(4/6) 1256 CCCGC Hin1I 1150 GGCGCC G[GA]^CG[CT]C 1333 GACGTC HincII 262 GTCAAC GT[CT]^[GA]AC 994 GTTAAC HindII 262 GTCAAC GT[CT]^[GA]AC 994 GTTAAC MaeII 197 ACGT A^CGT 1334 ACGT MflI 440 GGATCT [GA]^GATC[CT] 800 GGATCT NciI 446 CCCGG CC^[GC]GG 719 CCCGG NspI 535 ACATGT [GA]CATG^[CT] 1292 ACATGT SmlI 666 CTTGAG C^T[CT][GA]AG 893 CTCGAG XhoII 440 GGATCT [GA]^GATC[CT] 800 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BmyI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I