(Circular) (Six-base) MAPSORT of: pFN21AE2528.seq Check: 8989 from: 1 to: 6052 pFN21AE2528 6052 bp With 182 enzymes: SgfI * June 11, 2014 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2720 2845 2720 Size: 125 5927 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3342 3862 278 Size: 53 83 186 936 1806 520 2468 Fragments arranged by size: 2468 1806 936 520 186 83 53 AccI GT'mk_AC Cuts at: 1937 3124 3395 1937 Size: 1187 271 4594 Fragments arranged by size: 4594 1187 271 Acc65I G'GTAC_C Cuts at: 3373 3373 Size: 6052 AclI AA'CG_TT Cuts at: 1563 4179 4552 1563 Size: 2616 373 3063 Fragments arranged by size: 3063 2616 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2041 2103 4115 5499 5767 1632 Size: 409 62 2012 1384 268 1917 Fragments arranged by size: 2012 1917 1384 409 268 62 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5855 AflIII A'CryG_T Cuts at: 1203 2540 3297 4951 5928 1203 Size: 1337 757 1654 977 1327 Fragments arranged by size: 1654 1337 1327 977 757 AgeI A'CCGG_T Cuts at: 4883 4883 Size: 6052 AhdI GACnn_n'nnGTC Cuts at: 4781 4781 Size: 6052 AleI CACnn'nnGTG Cuts at: 1166 2864 1166 Size: 1698 4354 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6052 AlwNI CAG_nnn'CTG Cuts at: 3795 5367 3795 Size: 1572 4480 ApaI G_GGCC'C Cuts at: 2407 3116 2407 Size: 709 5343 ApaLI G'TGCA_C Cuts at: 4109 5265 4109 Size: 1156 4896 ApoI r'AATT_y Cuts at: 1493 2518 2705 3361 3587 3739 5848 1493 Size: 1025 187 656 226 152 2109 1697 Fragments arranged by size: 2109 1697 1025 656 226 187 152 AseI AT'TA_AT Cuts at: 160 1032 4606 160 Size: 872 3574 1606 Fragments arranged by size: 3574 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 AvaI C'yCGr_G Cuts at: 1870 1943 2898 3377 1870 Size: 73 955 479 4545 Fragments arranged by size: 4545 955 479 73 AvrII C'CTAG_G Cuts at: 2851 2851 Size: 6052 BamHI G'GATC_C Cuts at: 3382 3749 3382 Size: 367 5685 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3155 3373 4828 618 Size: 325 217 627 1368 218 1455 1842 Fragments arranged by size: 1842 1455 1368 627 325 218 217 BanII G_rGCy'C Cuts at: 1391 2407 2891 3116 3371 1391 Size: 1016 484 225 255 4072 Fragments arranged by size: 4072 1016 484 255 225 BbeI G_GCGC'C Cuts at: 3159 3159 Size: 6052 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5081 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4243 4277 1903 Size: 34 2306 34 3678 Fragments arranged by size: 3678 2306 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3944 5154 3944 Size: 1210 4842 BclI T'GATC_A Cuts at: 1548 1548 Size: 6052 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4663 136 Size: 107 122 71 631 605 2991 1525 Fragments arranged by size: 2991 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6047 6047 Size: 6052 BlpI GC'TnA_GC Cuts at: 3469 3469 Size: 6052 Bme1580I G_kGCm'C Cuts at: 1790 2407 3116 4113 5269 1790 Size: 617 709 997 1156 2573 Fragments arranged by size: 2573 1156 997 709 617 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6052 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2189 2638 3783 Size: 416 53 83 399 24 772 449 1145 Cuts at: 3783 4741 442 Size: 958 1753 Fragments arranged by size: 1753 1145 958 772 449 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6052 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2756 3096 4712 1239 Size: 1517 340 1616 2579 Fragments arranged by size: 2579 1616 1517 340 Bpu10I CC'TnA_GC Cuts at: 2441 2441 Size: 6052 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2692 4177 5042 5340 2692 Size: 1485 865 298 3404 Fragments arranged by size: 3404 1485 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2036 4715 882 Size: 617 537 2679 2219 Fragments arranged by size: 2679 2219 617 537 BsaAI yAC'GTr Cuts at: 493 1204 3843 493 Size: 711 2639 2702 Fragments arranged by size: 2702 2639 711 BsaBI GATnn'nnATC Cuts at: 6046 6046 Size: 6052 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3156 3339 3859 4241 Size: 53 83 186 936 1623 183 520 382 Cuts at: 4241 4876 275 Size: 635 1451 Fragments arranged by size: 1623 1451 936 635 520 382 186 183 83 53 BsaWI w'CCGG_w Cuts at: 2153 4485 4883 5157 5304 5715 2153 Size: 2332 398 274 147 411 2490 Fragments arranged by size: 2490 2332 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5827 Fragments arranged by size: 5827 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3224 1186 Size: 2038 4014 BseYI C'CCAG_C Cuts at: 2777 3115 5255 5751 2777 Size: 338 2140 496 3078 Fragments arranged by size: 3078 2140 496 338 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2636 2665 2817 3174 1163 Size: 573 900 29 152 357 4041 Fragments arranged by size: 4041 900 573 357 152 29 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3502 4263 5291 1540 Size: 275 186 1501 761 1028 2301 Fragments arranged by size: 2301 1501 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2871 2891 4113 4198 5269 2871 Size: 20 1222 85 1071 3654 Fragments arranged by size: 3654 1222 1071 85 20 BsmI GAATG_Cn' Cuts at: 3563 3656 3563 Size: 93 5959 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2407 2871 2891 3116 3371 4113 4198 Size: 399 617 464 20 225 255 742 85 Cuts at: 4198 5269 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 617 464 399 255 225 85 20 BspEI T'CCGG_A Cuts at: 2153 5715 2153 Size: 3562 2490 BspHI T'CATG_A Cuts at: 3941 5671 3941 Size: 1730 4322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2608 2720 2845 3407 844 Size: 1764 112 125 562 3489 Fragments arranged by size: 3489 1764 562 125 112 BsrBI CCG'CTC Cuts at: 1394 1823 2740 3939 1394 Size: 429 917 1199 3507 Fragments arranged by size: 3507 1199 917 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2238 2294 4547 4721 65 Size: 1183 990 56 2253 174 1396 Fragments arranged by size: 2253 1396 1183 990 174 56 BsrFI r'CCGG_y Cuts at: 1700 1810 4696 4883 1700 Size: 110 2886 187 2869 Fragments arranged by size: 2886 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 96 Size: 1911 4141 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6052 BssSI C'ACGA_G Cuts at: 2841 4112 5124 2841 Size: 1271 1012 3769 Fragments arranged by size: 3769 1271 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6052 BstBI TT'CG_AA Cuts at: 4856 4856 Size: 6052 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6052 BstYI r'GATC_y Cuts at: 1972 2445 2805 3382 3749 4134 4151 5603 5689 Size: 473 360 577 367 385 17 1452 86 Cuts at: 5689 5701 6047 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 577 473 385 367 360 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2253 2505 2526 3276 513 Size: 552 1188 252 21 750 3289 Fragments arranged by size: 3289 1188 750 552 252 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5313 Fragments arranged by size: 5313 683 56 BtsI GCAGTG_nn' Cuts at: 3568 4362 4382 3568 Size: 794 20 5238 Fragments arranged by size: 5238 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6052 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6017 DraI TTT'AAA Cuts at: 2185 3356 2185 Size: 1171 4881 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2264 5059 809 Size: 507 948 2795 1802 Fragments arranged by size: 2795 1802 948 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3499 4388 6017 Size: 54 1425 50 275 18 1669 889 1629 Cuts at: 6017 8 Size: 43 Fragments arranged by size: 1669 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3499 1537 Size: 275 1687 4090 Fragments arranged by size: 4090 1687 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2028 2655 3230 3982 1353 Size: 675 627 575 752 3423 Fragments arranged by size: 3423 752 675 627 575 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4639 5013 5159 387 Size: 1064 138 3050 374 146 1280 Fragments arranged by size: 3050 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2889 2889 Size: 6052 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 2103 2756 3096 4115 4712 5499 Size: 393 409 62 653 340 1019 597 787 Cuts at: 5499 5767 1239 Size: 268 1524 Fragments arranged by size: 1524 1019 787 653 597 409 393 340 268 62 EcoO109I rG'GnC_Cy Cuts at: 2189 2403 2189 Size: 214 5838 EcoRI G'AATT_C Cuts at: 3361 3739 3361 Size: 378 5674 FspI TGC'GCA Cuts at: 4558 4558 Size: 6052 HaeII r_GCGC'y Cuts at: 1678 3159 5199 1678 Size: 1481 2040 2531 Fragments arranged by size: 2531 2040 1481 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3128 3160 3242 3274 3324 Size: 32 135 32 1556 32 82 32 50 Cuts at: 3324 3356 4693 4725 4767 4799 1373 Size: 32 1337 32 42 32 2626 Fragments arranged by size: 2626 1556 1337 135 82 50 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2269 3001 3396 3642 669 Size: 988 281 331 732 395 246 3079 Fragments arranged by size: 3079 988 732 395 331 281 246 HindIII A'AGCT_T Cuts at: 748 2560 748 Size: 1812 4240 HpaI GTT'AAC Cuts at: 3001 3642 3001 Size: 641 5411 KasI G'GCGC_C Cuts at: 3155 3155 Size: 6052 KpnI G_GTAC'C Cuts at: 3377 3377 Size: 6052 MfeI C'AATT_G Cuts at: 3651 3651 Size: 6052 MluI A'CGCG_T Cuts at: 5928 5928 Size: 6052 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5166 5350 1305 Size: 3861 184 2007 Fragments arranged by size: 3861 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4230 Fragments arranged by size: 4230 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2412 2864 2945 4010 4369 4528 518 Size: 648 1246 452 81 1065 359 159 2042 Fragments arranged by size: 2042 1246 1065 648 452 359 159 81 MspA1I CmG'CkG Cuts at: 1606 3468 4145 5293 5538 1606 Size: 1862 677 1148 245 2120 Fragments arranged by size: 2120 1862 1148 677 245 NarI GG'CG_CC Cuts at: 3156 3156 Size: 6052 NcoI C'CATG_G Cuts at: 513 1065 2253 2505 2526 3276 513 Size: 552 1188 252 21 750 3289 Fragments arranged by size: 3289 1188 750 552 252 21 NdeI CA'TA_TG Cuts at: 387 387 Size: 6052 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6052 NotI GC'GGCC_GC Cuts at: 3499 3499 Size: 6052 NspI r_CATG'y Cuts at: 1132 1229 2544 3301 3410 4955 1132 Size: 97 1315 757 109 1545 2229 Fragments arranged by size: 2229 1545 1315 757 109 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6052 PciI A'CATG_T Cuts at: 2540 3297 4951 2540 Size: 757 1654 3641 Fragments arranged by size: 3641 1654 757 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6052 PfoI T'CCnGG_A Cuts at: 2127 2450 2127 Size: 323 5729 PmeI GTTT'AAAC Cuts at: 3356 3356 Size: 6052 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2711 2743 4121 4153 5634 5666 2711 Size: 32 1378 32 1481 32 3097 Fragments arranged by size: 3097 1481 1378 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2189 2189 Size: 6052 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6052 PsiI TTA'TAA Cuts at: 3622 3622 Size: 6052 PspOMI G'GGCC_C Cuts at: 2403 3112 2403 Size: 709 5343 PstI C_TGCA'G Cuts at: 830 3404 830 Size: 2574 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6052 SacI G_AGCT'C Cuts at: 2891 2891 Size: 6052 SalI G'TCGA_C Cuts at: 1936 3394 1936 Size: 1458 4594 SbfI CC_TGCA'GG Cuts at: 3404 3404 Size: 6052 ScaI AGT'ACT Cuts at: 4300 6012 4300 Size: 1712 4340 SexAI A'CCwGG_T Cuts at: 3348 3348 Size: 6052 SfcI C'TryA_G Cuts at: 826 1045 2260 2470 3125 3400 4535 5216 5407 Size: 219 1215 210 655 275 1135 681 191 Cuts at: 5407 826 Size: 1471 Fragments arranged by size: 1471 1215 1135 681 655 275 219 210 191 SfoI GGC'GCC Cuts at: 3157 3157 Size: 6052 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6052 SmaI CCC'GGG Cuts at: 1872 3379 1872 Size: 1507 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2671 2898 4156 5057 5319 820 Size: 197 926 728 227 1258 901 262 1553 Fragments arranged by size: 1553 1258 926 901 728 262 227 197 SnaBI TAC'GTA Cuts at: 493 3843 493 Size: 3350 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6052 SphI G_CATG'C Cuts at: 1132 3410 1132 Size: 2278 3774 SspI AAT'ATT Cuts at: 5 52 3976 5 Size: 47 3924 2081 Fragments arranged by size: 3924 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6052 StyI C'CwwG_G Cuts at: 513 1065 2253 2505 2526 2851 2913 3132 3276 Size: 552 1188 252 21 325 62 219 144 Cuts at: 3276 3491 513 Size: 215 3074 Fragments arranged by size: 3074 1188 552 325 252 219 215 144 62 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2116 2897 4079 4096 4249 4434 4879 Size: 1255 536 781 1182 17 153 185 445 Cuts at: 4879 325 Size: 1498 Fragments arranged by size: 1498 1255 1182 781 536 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 4298 6010 Size: 274 80 33 51 1443 30 2291 1712 Cuts at: 6010 96 Size: 138 Fragments arranged by size: 2291 1712 1443 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6012 6044 6012 Size: 32 6020 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6052 XbaI T'CTAG_A Cuts at: 3388 3388 Size: 6052 XhoI C'TCGA_G Cuts at: 1943 2898 1943 Size: 955 5097 XmaI C'CCGG_G Cuts at: 1870 3377 1870 Size: 1507 4545 XmnI GAAnn'nnTTC Cuts at: 2519 4181 2519 Size: 1662 4390 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3340 3860 276 Size: 53 83 186 936 1806 520 2468 Fragments arranged by size: 2468 1806 936 520 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI