(Circular) (Six-base) MAPSORT of: pFN21AE1887.seq Check: 292 from: 1 to: 5944 pFN21AE1887 5944 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2076 3754 278 Size: 53 83 186 936 540 1678 2468 Fragments arranged by size: 2468 1678 936 540 186 83 53 AccI GT'mk_AC Cuts at: 1937 2658 3287 1937 Size: 721 629 4594 Fragments arranged by size: 4594 721 629 Acc65I G'GTAC_C Cuts at: 3265 3265 Size: 5944 AclI AA'CG_TT Cuts at: 1563 4071 4444 1563 Size: 2508 373 3063 Fragments arranged by size: 3063 2508 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4007 5391 5659 1632 Size: 2375 1384 268 1917 Fragments arranged by size: 2375 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5747 AflIII A'CryG_T Cuts at: 1203 4843 5820 1203 Size: 3640 977 1327 Fragments arranged by size: 3640 1327 977 AgeI A'CCGG_T Cuts at: 4775 4775 Size: 5944 AhdI GACnn_n'nnGTC Cuts at: 4673 4673 Size: 5944 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5944 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5944 AlwNI CAG_nnn'CTG Cuts at: 2434 3687 5259 2434 Size: 1253 1572 3119 Fragments arranged by size: 3119 1572 1253 ApaLI G'TGCA_C Cuts at: 4001 5157 4001 Size: 1156 4788 ApoI r'AATT_y Cuts at: 1493 3253 3479 3631 5740 1493 Size: 1760 226 152 2109 1697 Fragments arranged by size: 2109 1760 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4498 160 Size: 872 3466 1606 Fragments arranged by size: 3466 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2454 3269 1870 Size: 73 72 439 815 4545 Fragments arranged by size: 4545 815 439 73 72 BamHI G'GATC_C Cuts at: 3274 3641 3274 Size: 367 5577 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 2263 3265 4720 618 Size: 325 217 627 254 222 1002 1455 1842 Fragments arranged by size: 1842 1455 1002 627 325 254 222 217 BanII G_rGCy'C Cuts at: 1391 2993 3263 1391 Size: 1602 270 4072 Fragments arranged by size: 4072 1602 270 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2644 3030 3147 3165 928 Size: 971 745 386 117 18 3707 Fragments arranged by size: 3707 971 745 386 117 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2098 2132 4135 4169 1903 Size: 34 161 34 2003 34 3678 Fragments arranged by size: 3678 2003 161 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3836 5046 3836 Size: 1210 4734 BclI T'GATC_A Cuts at: 1548 2460 2643 1548 Size: 912 183 4849 Fragments arranged by size: 4849 912 183 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4555 136 Size: 107 122 71 631 605 2883 1525 Fragments arranged by size: 2883 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2783 5939 2783 Size: 3156 2788 BlpI GC'TnA_GC Cuts at: 3361 3361 Size: 5944 Bme1580I G_kGCm'C Cuts at: 1790 2268 2846 4005 5161 1790 Size: 478 578 1159 1156 2573 Fragments arranged by size: 2573 1159 1156 578 478 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5944 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3675 4633 442 Size: 416 53 83 399 24 2258 958 1753 Fragments arranged by size: 2258 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5944 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2647 2678 3052 4604 1239 Size: 1408 31 374 1552 2579 Fragments arranged by size: 2579 1552 1408 374 31 Bpu10I CC'TnA_GC Cuts at: 2738 2738 Size: 5944 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2804 4069 4934 5232 2804 Size: 1265 865 298 3516 Fragments arranged by size: 3516 1265 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2363 2561 4607 882 Size: 617 864 198 2046 2219 Fragments arranged by size: 2219 2046 864 617 198 BsaAI yAC'GTr Cuts at: 493 1204 3106 3735 493 Size: 711 1902 629 2702 Fragments arranged by size: 2702 1902 711 629 BsaBI GATnn'nnATC Cuts at: 5938 5938 Size: 5944 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 3751 4133 4768 Size: 53 83 186 936 540 1678 382 635 Cuts at: 4768 275 Size: 1451 Fragments arranged by size: 1678 1451 936 635 540 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4377 4775 5049 5196 5607 4377 Size: 398 274 147 411 4714 Fragments arranged by size: 4714 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3008 3038 3205 3235 1178 Size: 30 165 30 1605 30 167 30 3887 Fragments arranged by size: 3887 1605 167 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5944 BseYI C'CCAG_C Cuts at: 5147 5643 5147 Size: 496 5448 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5371 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3394 4155 5183 1540 Size: 275 186 1393 761 1028 2301 Fragments arranged by size: 2301 1393 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2811 4005 4090 5161 2811 Size: 1194 85 1071 3594 Fragments arranged by size: 3594 1194 1071 85 BsmI GAATG_Cn' Cuts at: 3455 3548 3455 Size: 93 5851 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2268 2811 2846 2993 3263 4005 4090 Size: 399 478 543 35 147 270 742 85 Cuts at: 4090 5161 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 543 478 399 270 147 85 35 BspEI T'CCGG_A Cuts at: 5607 5607 Size: 5944 BspHI T'CATG_A Cuts at: 3833 5563 3833 Size: 1730 4214 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2579 3024 3299 844 Size: 1735 445 275 3489 Fragments arranged by size: 3489 1735 445 275 BsrBI CCG'CTC Cuts at: 1394 1823 3831 1394 Size: 429 2008 3507 Fragments arranged by size: 3507 2008 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2161 2175 2604 3064 4439 4613 65 Size: 1183 913 14 429 460 1375 174 1396 Fragments arranged by size: 1396 1375 1183 913 460 429 174 14 BsrFI r'CCGG_y Cuts at: 1700 1810 4588 4775 1700 Size: 110 2778 187 2869 Fragments arranged by size: 2869 2778 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5944 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5944 BssSI C'ACGA_G Cuts at: 4004 5016 4004 Size: 1012 4932 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5944 BstBI TT'CG_AA Cuts at: 4748 4748 Size: 5944 BstEII G'GTnAC_C Cuts at: 1184 2203 1184 Size: 1019 4925 BstYI r'GATC_y Cuts at: 1972 2783 3274 3641 4026 4043 5495 5581 5593 Size: 811 491 367 385 17 1452 86 12 Cuts at: 5593 5939 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 811 491 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4453 Fragments arranged by size: 4453 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5205 Fragments arranged by size: 5205 683 56 BtsI GCAGTG_nn' Cuts at: 2265 2394 3236 3460 4254 4274 2265 Size: 129 842 224 794 20 3935 Fragments arranged by size: 3935 842 794 224 129 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5944 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5909 DraI TTT'AAA Cuts at: 3248 3248 Size: 5944 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2072 4951 809 Size: 507 756 2879 1802 Fragments arranged by size: 2879 1802 756 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 2358 3391 Size: 54 1425 50 275 18 309 219 1033 Cuts at: 3391 4280 5909 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1033 889 309 275 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3391 1537 Size: 275 1579 4090 Fragments arranged by size: 4090 1579 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2617 3874 1353 Size: 1264 1257 3423 Fragments arranged by size: 3423 1264 1257 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2776 4531 4905 5051 387 Size: 1064 138 1187 1755 374 146 1280 Fragments arranged by size: 1755 1280 1187 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2647 2678 3052 4007 4604 5391 5659 Size: 393 1015 31 374 955 597 787 268 Cuts at: 5659 1239 Size: 1524 Fragments arranged by size: 1524 1015 955 787 597 393 374 268 31 EcoNI CCTnn'n_nnAGG Cuts at: 2741 2741 Size: 5944 EcoRI G'AATT_C Cuts at: 3253 3631 3253 Size: 378 5566 EcoRV GAT'ATC Cuts at: 3101 3101 Size: 5944 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2815 2815 Size: 5944 FspI TGC'GCA Cuts at: 3032 4450 3032 Size: 1418 4526 HaeII r_GCGC'y Cuts at: 1678 2031 5091 1678 Size: 353 3060 2531 Fragments arranged by size: 3060 2531 353 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3085 3117 4585 4617 4659 Size: 32 135 32 1513 32 1468 32 42 Cuts at: 4659 4691 1373 Size: 32 2626 Fragments arranged by size: 2626 1513 1468 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2881 3288 3534 669 Size: 988 281 943 407 246 3079 Fragments arranged by size: 3079 988 943 407 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5944 HpaI GTT'AAC Cuts at: 2881 3534 2881 Size: 653 5291 KpnI G_GTAC'C Cuts at: 3269 3269 Size: 5944 MfeI C'AATT_G Cuts at: 3543 3543 Size: 5944 MluI A'CGCG_T Cuts at: 5820 5820 Size: 5944 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5058 5242 1305 Size: 3753 184 2007 Fragments arranged by size: 3753 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2141 10 Size: 54 1425 343 309 3813 Fragments arranged by size: 3813 1425 343 309 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 2342 2468 2513 2837 3902 4261 Size: 648 954 222 126 45 324 1065 359 Cuts at: 4261 4420 518 Size: 159 2042 Fragments arranged by size: 2042 1065 954 648 359 324 222 159 126 45 MspA1I CmG'CkG Cuts at: 1606 3360 4037 5185 5430 1606 Size: 1754 677 1148 245 2120 Fragments arranged by size: 2120 1754 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4453 Fragments arranged by size: 4453 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5944 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5944 NotI GC'GGCC_GC Cuts at: 3391 3391 Size: 5944 NspI r_CATG'y Cuts at: 1132 1229 2894 3302 4847 1132 Size: 97 1665 408 1545 2229 Fragments arranged by size: 2229 1665 1545 408 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5944 PciI A'CATG_T Cuts at: 4843 4843 Size: 5944 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5944 PfoI T'CCnGG_A Cuts at: 2898 3012 2898 Size: 114 5830 PmeI GTTT'AAAC Cuts at: 3248 3248 Size: 5944 PmlI CAC'GTG Cuts at: 3106 3106 Size: 5944 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4013 4045 5526 5558 4013 Size: 32 1481 32 4399 Fragments arranged by size: 4399 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5944 PsiI TTA'TAA Cuts at: 3514 3514 Size: 5944 PstI C_TGCA'G Cuts at: 830 2199 3296 830 Size: 1369 1097 3478 Fragments arranged by size: 3478 1369 1097 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5944 SalI G'TCGA_C Cuts at: 1936 3286 1936 Size: 1350 4594 SbfI CC_TGCA'GG Cuts at: 3296 3296 Size: 5944 ScaI AGT'ACT Cuts at: 4192 5904 4192 Size: 1712 4232 SfcI C'TryA_G Cuts at: 826 1045 2195 3292 4427 5108 5299 826 Size: 219 1150 1097 1135 681 191 1471 Fragments arranged by size: 1471 1150 1135 1097 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5944 SmaI CCC'GGG Cuts at: 1872 2017 2456 3271 1872 Size: 145 439 815 4545 Fragments arranged by size: 4545 815 439 145 SmlI C'TyrA_G Cuts at: 820 1017 1943 2819 4048 4949 5211 820 Size: 197 926 876 1229 901 262 1553 Fragments arranged by size: 1553 1229 926 901 876 262 197 SnaBI TAC'GTA Cuts at: 493 3735 493 Size: 3242 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5944 SphI G_CATG'C Cuts at: 1132 3302 1132 Size: 2170 3774 SspI AAT'ATT Cuts at: 5 52 3868 5 Size: 47 3816 2081 Fragments arranged by size: 3816 2081 47 StuI AGG'CCT Cuts at: 1517 2490 3050 1517 Size: 973 560 4411 Fragments arranged by size: 4411 973 560 StyI C'CwwG_G Cuts at: 513 1065 2004 3383 513 Size: 552 939 1379 3074 Fragments arranged by size: 3074 1379 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2059 2089 2549 3971 3988 4141 4326 Size: 1255 479 30 460 1422 17 153 185 Cuts at: 4326 4771 325 Size: 445 1498 Fragments arranged by size: 1498 1422 1255 479 460 445 185 153 30 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3224 4190 5902 Size: 274 80 33 51 1443 1247 966 1712 Cuts at: 5902 96 Size: 138 Fragments arranged by size: 1712 1443 1247 966 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2081 2113 3008 3040 3090 3122 5904 5936 2081 Size: 32 895 32 50 32 2782 32 2089 Fragments arranged by size: 2782 2089 895 50 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5944 XbaI T'CTAG_A Cuts at: 3280 3280 Size: 5944 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5944 XmaI C'CCGG_G Cuts at: 1870 2015 2454 3269 1870 Size: 145 439 815 4545 Fragments arranged by size: 4545 815 439 145 XmnI GAAnn'nnTTC Cuts at: 4073 4073 Size: 5944 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2074 3752 276 Size: 53 83 186 936 540 1678 2468 Fragments arranged by size: 2468 1678 936 540 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI