Restriction map for
pFN21AE1887
(Linear) (Six-base) MAPSORT of: KIEE1887.seq Check: 1792 from: 1 to: 1239
KIEE1887.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AatII G_ACGT'C
Cuts at: 0 71 1239
Size: 71 1168
AccI GT'mk_AC
Cuts at: 0 653 1239
Size: 653 586
AlwNI CAG_nnn'CTG
Cuts at: 0 429 1239
Size: 429 810
AvaI C'yCGr_G
Cuts at: 0 10 449 1239
Size: 10 439 790
Fragments arranged by size:
790 439 10
BanI G'GyrC_C
Cuts at: 0 36 258 1239
Size: 36 222 981
Fragments arranged by size:
981 222 36
BanII G_rGCy'C
Cuts at: 0 988 1239
Size: 988 251
BbsI GAAGACnn'nnnn_
Cuts at: 0 639 1025 1142 1160 1239
Size: 639 386 117 18 79
Fragments arranged by size:
639 386 117 79 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 93 127 1239
Size: 93 34 1112
Fragments arranged by size:
1112 93 34
BclI T'GATC_A
Cuts at: 0 455 638 1239
Size: 455 183 601
Fragments arranged by size:
601 455 183
BglII A'GATC_T
Cuts at: 0 778 1239
Size: 778 461
Bme1580I G_kGCm'C
Cuts at: 0 263 841 1239
Size: 263 578 398
Fragments arranged by size:
578 398 263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 673 1047 1239
Size: 642 31 374 192
Fragments arranged by size:
642 374 192 31
Bpu10I CC'TnA_GC
Cuts at: 0 733 1239
Size: 733 506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1239
Size: 799 440
BsaI GGTCTCn'nnnn_
Cuts at: 0 358 556 1239
Size: 358 198 683
Fragments arranged by size:
683 358 198
BsaAI yAC'GTr
Cuts at: 0 1101 1239
Size: 1101 138
BsaHI Gr'CG_yC
Cuts at: 0 68 1239
Size: 68 1171
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1003 1033 1200 1230 1239
Size: 1003 30 167 30 9
Fragments arranged by size:
1003 167 30 30 9
BsiHKAI G_wGCw'C
Cuts at: 0 806 1239
Size: 806 433
Bsp1286I G_dGCh'C
Cuts at: 0 263 806 841 988 1239
Size: 263 543 35 147 251
Fragments arranged by size:
543 263 251 147 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 574 1019 1239
Size: 574 445 220
Fragments arranged by size:
574 445 220
BsrDI GCAATG_nn'
Cuts at: 0 156 170 599 1059 1239
Size: 156 14 429 460 180
Fragments arranged by size:
460 429 180 156 14
BstEII G'GTnAC_C
Cuts at: 0 198 1239
Size: 198 1041
BstYI r'GATC_y
Cuts at: 0 778 1239
Size: 778 461
BtsI GCAGTG_nn'
Cuts at: 0 260 389 1231 1239
Size: 260 129 842 8
Fragments arranged by size:
842 260 129 8
DrdI GACnn_nn'nnGTC
Cuts at: 0 67 1239
Size: 67 1172
EaeI y'GGCC_r
Cuts at: 0 134 353 1239
Size: 134 219 886
Fragments arranged by size:
886 219 134
EarI CTCTTCn'nnn_
Cuts at: 0 612 1239
Size: 612 627
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 771 1239
Size: 771 468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 673 1047 1239
Size: 642 31 374 192
Fragments arranged by size:
642 374 192 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 736 1239
Size: 736 503
EcoRV GAT'ATC
Cuts at: 0 1096 1239
Size: 1096 143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 810 1239
Size: 810 429
FspI TGC'GCA
Cuts at: 0 1027 1239
Size: 1027 212
HaeII r_GCGC'y
Cuts at: 0 26 1239
Size: 26 1213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1080 1112 1239
Size: 1080 32 127
Fragments arranged by size:
1080 127 32
HincII GTy'rAC
Cuts at: 0 876 1239
Size: 876 363
HpaI GTT'AAC
Cuts at: 0 876 1239
Size: 876 363
MscI TGG'CCA
Cuts at: 0 136 1239
Size: 136 1103
MslI CAynn'nnrTG
Cuts at: 0 115 337 463 508 832 1239
Size: 115 222 126 45 324 407
Fragments arranged by size:
407 324 222 126 115 45
NspI r_CATG'y
Cuts at: 0 889 1239
Size: 889 350
PfoI T'CCnGG_A
Cuts at: 0 893 1007 1239
Size: 893 114 232
Fragments arranged by size:
893 232 114
PmlI CAC'GTG
Cuts at: 0 1101 1239
Size: 1101 138
PstI C_TGCA'G
Cuts at: 0 194 1239
Size: 194 1045
SfcI C'TryA_G
Cuts at: 0 190 1239
Size: 190 1049
SmaI CCC'GGG
Cuts at: 0 12 451 1239
Size: 12 439 788
Fragments arranged by size:
788 439 12
SmlI C'TyrA_G
Cuts at: 0 814 1239
Size: 814 425
StuI AGG'CCT
Cuts at: 0 485 1045 1239
Size: 485 560 194
Fragments arranged by size:
560 485 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 54 84 544 1239
Size: 54 30 460 695
Fragments arranged by size:
695 460 54 30
TatI w'GTAC_w
Cuts at: 0 1219 1239
Size: 1219 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 76 108 1003 1035 1085 1117 1239
Size: 76 32 895 32 50 32 122
Fragments arranged by size:
895 122 76 50 32 32 32
XmaI C'CCGG_G
Cuts at: 0 10 449 1239
Size: 10 439 790
Fragments arranged by size:
790 439 10
ZraI GAC'GTC
Cuts at: 0 69 1239
Size: 69 1170
Enzymes that do cut:
AatII AccI AlwNI AvaI BanI BanII BbsI BcgI
BclI BglII Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BsiHKAI Bsp1286I BspMI BsrDI BstEII BstYI
BtsI DrdI EaeI EarI EciI Eco57MI EcoNI EcoRV
FalI FspI HaeII Hin4I HincII HpaI MscI MslI
NspI PfoI PmlI PstI SfcI SmaI SmlI StuI
TaqII TatI TstI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI BsaBI
BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII EagI EcoICRI EcoO109I EcoRI FseI FspAI
HindIII KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StyI SwaI Tth111I XbaI XcmI XhoI XmnI