(Circular) (Six-base) MAPSORT of: pFN21AE2359.seq Check: 3367 from: 1 to: 5866 pFN21AE2359 5866 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2597 2869 2597 Size: 272 5594 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2307 3676 278 Size: 53 83 186 936 771 1369 2468 Fragments arranged by size: 2468 1369 936 771 186 83 53 AccI GT'mk_AC Cuts at: 1937 3209 1937 Size: 1272 4594 Acc65I G'GTAC_C Cuts at: 3187 3187 Size: 5866 AclI AA'CG_TT Cuts at: 1563 3993 4366 1563 Size: 2430 373 3063 Fragments arranged by size: 3063 2430 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3929 5313 5581 1632 Size: 2297 1384 268 1917 Fragments arranged by size: 2297 1917 1384 268 AfeI AGC'GCT Cuts at: 3002 3002 Size: 5866 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5669 AflIII A'CryG_T Cuts at: 1203 3070 4765 5742 1203 Size: 1867 1695 977 1327 Fragments arranged by size: 1867 1695 1327 977 AgeI A'CCGG_T Cuts at: 4697 4697 Size: 5866 AhdI GACnn_n'nnGTC Cuts at: 4595 4595 Size: 5866 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5866 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5866 AlwNI CAG_nnn'CTG Cuts at: 3609 5181 3609 Size: 1572 4294 ApaLI G'TGCA_C Cuts at: 3923 5079 3923 Size: 1156 4710 ApoI r'AATT_y Cuts at: 1493 3175 3401 3553 5662 1493 Size: 1682 226 152 2109 1697 Fragments arranged by size: 2109 1697 1682 226 152 AseI AT'TA_AT Cuts at: 160 1032 2423 3057 4420 160 Size: 872 1391 634 1363 1606 Fragments arranged by size: 1606 1391 1363 872 634 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 3116 3191 1870 Size: 73 1173 75 4545 Fragments arranged by size: 4545 1173 75 73 BamHI G'GATC_C Cuts at: 2848 3062 3196 3563 2848 Size: 214 134 367 5151 Fragments arranged by size: 5151 367 214 134 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2234 2821 3187 4642 618 Size: 325 217 627 447 587 366 1455 1842 Fragments arranged by size: 1842 1455 627 587 447 366 325 217 BanII G_rGCy'C Cuts at: 1391 3185 1391 Size: 1794 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2551 3016 928 Size: 971 652 465 3778 Fragments arranged by size: 3778 971 652 465 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 1903 Size: 34 2120 34 3678 Fragments arranged by size: 3678 2120 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3758 4968 3758 Size: 1210 4656 BclI T'GATC_A Cuts at: 1548 1548 Size: 5866 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4477 136 Size: 107 122 71 631 605 2805 1525 Fragments arranged by size: 2805 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5861 5861 Size: 5866 BlpI GC'TnA_GC Cuts at: 3283 3283 Size: 5866 Bme1580I G_kGCm'C Cuts at: 1790 2824 3927 5083 1790 Size: 1034 1103 1156 2573 Fragments arranged by size: 2573 1156 1103 1034 BmgBI CAC'GTC Cuts at: 1318 2966 1318 Size: 1648 4218 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3597 4555 442 Size: 416 53 83 399 24 2180 958 1753 Fragments arranged by size: 2180 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2739 2739 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4526 1239 Size: 3287 2579 Bpu10I CC'TnA_GC Cuts at: 2153 2661 2153 Size: 508 5358 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3991 4856 5154 3991 Size: 865 298 4703 Fragments arranged by size: 4703 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2759 4529 882 Size: 617 1260 1770 2219 Fragments arranged by size: 2219 1770 1260 617 BsaAI yAC'GTr Cuts at: 493 1204 2550 3657 493 Size: 711 1346 1107 2702 Fragments arranged by size: 2702 1346 1107 711 BsaBI GATnn'nnATC Cuts at: 2102 5860 2102 Size: 3758 2108 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2304 3673 4055 4690 Size: 53 83 186 936 771 1369 382 635 Cuts at: 4690 275 Size: 1451 Fragments arranged by size: 1451 1369 936 771 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4299 4697 4971 5118 5529 4299 Size: 398 274 147 411 4636 Fragments arranged by size: 4636 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5641 Fragments arranged by size: 5641 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2612 2698 1186 Size: 865 561 86 4354 Fragments arranged by size: 4354 865 561 86 BseYI C'CCAG_C Cuts at: 2139 2428 2439 2817 5069 5565 2139 Size: 289 11 378 2252 496 2440 Fragments arranged by size: 2440 2252 496 378 289 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3077 1163 Size: 573 1341 3952 Fragments arranged by size: 3952 1341 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3316 4077 5105 1540 Size: 275 186 1315 761 1028 2301 Fragments arranged by size: 2301 1315 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3927 4012 5083 3927 Size: 85 1071 4710 Fragments arranged by size: 4710 1071 85 BsmI GAATG_Cn' Cuts at: 3377 3470 3377 Size: 93 5773 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2824 3185 3927 4012 5083 1391 Size: 399 1034 361 742 85 1071 2174 Fragments arranged by size: 2174 1071 1034 742 399 361 85 BspEI T'CCGG_A Cuts at: 5529 5529 Size: 5866 BspHI T'CATG_A Cuts at: 3755 5485 3755 Size: 1730 4136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2124 2597 2869 3221 844 Size: 1280 473 272 352 3489 Fragments arranged by size: 3489 1280 473 352 272 BsrBI CCG'CTC Cuts at: 1394 1823 3753 1394 Size: 429 1930 3507 Fragments arranged by size: 3507 1930 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4361 4535 65 Size: 1183 3113 174 1396 Fragments arranged by size: 3113 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4510 4697 1700 Size: 110 2700 187 2869 Fragments arranged by size: 2869 2700 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5866 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5866 BssSI C'ACGA_G Cuts at: 3926 4938 3926 Size: 1012 4854 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5866 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5866 BstXI CCAn_nnnn'nTGG Cuts at: 2245 2845 2245 Size: 600 5266 BstYI r'GATC_y Cuts at: 1972 2200 2679 2848 3062 3196 3563 3948 3965 Size: 228 479 169 214 134 367 385 17 Cuts at: 3965 5417 5503 5515 5861 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 479 385 367 346 228 214 169 134 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4375 Fragments arranged by size: 4375 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2401 2735 487 Size: 683 56 1175 334 3618 Fragments arranged by size: 3618 1175 683 334 56 BtsI GCAGTG_nn' Cuts at: 3382 4176 4196 3382 Size: 794 20 5052 Fragments arranged by size: 5052 794 20 ClaI AT'CG_AT Cuts at: 1554 2324 1554 Size: 770 5096 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5831 DraI TTT'AAA Cuts at: 3170 3170 Size: 5866 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2867 4873 809 Size: 507 1551 2006 1802 Fragments arranged by size: 2006 1802 1551 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 3313 4202 Size: 54 1425 50 275 18 270 1213 889 Cuts at: 4202 5831 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1213 889 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3313 1537 Size: 275 1501 4090 Fragments arranged by size: 4090 1501 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3796 1353 Size: 2443 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4453 4827 4973 387 Size: 1064 138 2864 374 146 1280 Fragments arranged by size: 2864 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3929 4526 5313 5581 1239 Size: 393 2297 597 787 268 1524 Fragments arranged by size: 2297 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3175 3553 3175 Size: 378 5488 FspI TGC'GCA Cuts at: 2414 4372 2414 Size: 1958 3908 HaeII r_GCGC'y Cuts at: 1678 3004 5013 1678 Size: 1326 2009 2531 Fragments arranged by size: 2531 2009 1326 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2392 2424 3131 3163 4507 Size: 32 135 32 820 32 707 32 1344 Cuts at: 4507 4539 4581 4613 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1344 820 707 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2569 3210 3456 669 Size: 988 281 631 641 246 3079 Fragments arranged by size: 3079 988 641 631 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5866 HpaI GTT'AAC Cuts at: 3456 3456 Size: 5866 KpnI G_GTAC'C Cuts at: 3191 3191 Size: 5866 MfeI C'AATT_G Cuts at: 3465 3465 Size: 5866 MluI A'CGCG_T Cuts at: 5742 5742 Size: 5866 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4980 5164 1305 Size: 3675 184 2007 Fragments arranged by size: 3675 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2102 10 Size: 54 1425 343 270 3774 Fragments arranged by size: 3774 1425 343 270 54 MslI CAynn'nnrTG Cuts at: 518 1166 2795 3824 4183 4342 518 Size: 648 1629 1029 359 159 2042 Fragments arranged by size: 2042 1629 1029 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3282 3959 5107 5352 1606 Size: 1676 677 1148 245 2120 Fragments arranged by size: 2120 1676 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4375 Fragments arranged by size: 4375 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5866 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NspI r_CATG'y Cuts at: 1132 1229 2672 3074 3224 4769 1132 Size: 97 1443 402 150 1545 2229 Fragments arranged by size: 2229 1545 1443 402 150 97 PasI CC'CwG_GG Cuts at: 1348 2873 1348 Size: 1525 4341 PciI A'CATG_T Cuts at: 3070 4765 3070 Size: 1695 4171 PflMI CCAn_nnn'nTGG Cuts at: 1108 2383 1108 Size: 1275 4591 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2528 2560 3935 3967 5448 5480 2528 Size: 32 1375 32 1481 32 2914 Fragments arranged by size: 2914 1481 1375 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5866 PsiI TTA'TAA Cuts at: 3436 3436 Size: 5866 PstI C_TGCA'G Cuts at: 830 3218 830 Size: 2388 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5866 SalI G'TCGA_C Cuts at: 1936 3208 1936 Size: 1272 4594 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 4114 5826 4114 Size: 1712 4154 SfcI C'TryA_G Cuts at: 826 1045 2389 3214 4349 5030 5221 826 Size: 219 1344 825 1135 681 191 1471 Fragments arranged by size: 1471 1344 1135 825 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5866 SmaI CCC'GGG Cuts at: 1872 3193 1872 Size: 1321 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3116 3970 4871 5133 820 Size: 197 926 1173 854 901 262 1553 Fragments arranged by size: 1553 1173 926 901 854 262 197 SnaBI TAC'GTA Cuts at: 493 2550 3657 493 Size: 2057 1107 2702 Fragments arranged by size: 2702 2057 1107 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 2672 3224 1132 Size: 1540 552 3774 Fragments arranged by size: 3774 1540 552 SspI AAT'ATT Cuts at: 5 52 2842 3790 5 Size: 47 2790 948 2081 Fragments arranged by size: 2790 2081 948 47 StuI AGG'CCT Cuts at: 1517 2722 1517 Size: 1205 4661 StyI C'CwwG_G Cuts at: 513 1065 2004 3305 513 Size: 552 939 1301 3074 Fragments arranged by size: 3074 1301 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3893 3910 4063 4248 4693 325 Size: 1255 2313 17 153 185 445 1498 Fragments arranged by size: 2313 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3073 4112 5824 Size: 274 80 33 51 1443 1096 1039 1712 Cuts at: 5824 96 Size: 138 Fragments arranged by size: 1712 1443 1096 1039 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5826 5858 5826 Size: 32 5834 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5866 XbaI T'CTAG_A Cuts at: 3202 3202 Size: 5866 XhoI C'TCGA_G Cuts at: 1943 3116 1943 Size: 1173 4693 XmaI C'CCGG_G Cuts at: 1870 3191 1870 Size: 1321 4545 XmnI GAAnn'nnTTC Cuts at: 3013 3995 3013 Size: 982 4884 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2305 3674 276 Size: 53 83 186 936 771 1369 2468 Fragments arranged by size: 2468 1369 936 771 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI